LemaA.01010.a

Putative mitogen-activated protein kinase kinase 3

CENTER ID: LemaA.01010.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.01010.a.HZ2.GE43717 kinase domain (Jensen) 22 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.17.0060
UniProt: Q4QEJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFYALQDSL LRNAKSGGSS DTGDVESRYV LMEKIGSGSY GDVWSATRRT GAKDIYAVKR IDKRQAGTKG LRSVMGEVET MSLLSHPNIV KLEETYQDEA CLSIVMEYLP GGSLQHRIDR QSVSELETRR FITQLLMAVE YIHEKGIVHR DLKPSNCLLS QNDLVVKISD FGLSVFAGSK QCLTTCCGTL HFMAPEILLE KNYGKPVDMW AMGVMAHVMF LGCYPFQART EAALTKEICR GYRPREEGAG GLRCPPLLLD FISQLLLYDP HRRMSAKDAL KHPWIKEGLE ANRRTSLYPS QAPSATAMKP SARWRAAVLA VMGVQRLLYL QKIQRLVRLG YDGFPILRDY RYLVTGKYVP ASTSVECSHM FHARPMALLE LISMIDTCPF LKHVDLSWNN IHSLSVIQSL LKVVTRHPSL QSIDLSHNPI PAVAGRSIVR LIRNPLSQVT CINVGDTGIS ADTIGQINSF LKEKLTPAAL SYSVSTGDMQ SRLTQGSGDD ALMTSSLTMV SSLQQKRSIG SGPPKQPAKK ESRGVRLPPI SRPPVARRHG KMP
NT Sequence
atggacttct acgctttgca agacagtctc ttgcgcaatg ccaagagcgg aggcagctct gacaccggcg acgtcgagtc gaggtacgtc ttgatggaga aaatcggctc tggctcttat ggcgatgtct ggagcgcgac tcggcgcaca ggggcgaagg acatctacgc ggtgaagcgc atcgacaagc gtcaggcggg caccaaggga ctgcggagcg tcatggggga agtggagacg atgagccttc tttctcatcc caatatcgtc aagctggagg agacgtatca agacgaagct tgcctatcaa ttgtgatgga gtacctccct ggcggttccc ttcagcaccg gatagaccgg caaagcgtgt ctgagctgga gacgcggcgc ttcatcacgc agctgctgat ggctgtcgag tacattcatg aaaaaggtat cgtccaccgc gatttaaagc cgagcaactg cctactgtct caaaacgacc tcgtcgtaaa gatatcagac ttcgggctct ccgtgtttgc cggcagtaag caatgcttga cgacgtgctg tggcacgctg cactttatgg cacccgagat tctgctcgag aaaaactacg gcaagccggt ggacatgtgg gccatgggcg tcatggcaca cgtgatgttt ttaggttgtt acccatttca ggcccgtaca gaggcggcgc tgacaaagga gatctgccgc gggtatcgtc cgcgggaaga gggtgctggt gggctgcgat gtccaccact gctactggac tttatcagtc agcttctcct ttacgaccca caccgtcgaa tgagcgccaa agacgctctc aagcacccat ggatcaagga aggcttagaa gccaaccggc ggacatccct ctacccgtca caggcaccct cggcgacagc gatgaagcca tctgcgcggt ggcgagcggc ggtacttgca gtcatgggcg tgcagcggct gctctacttg cagaaaattc agcggctggt gcggctcggt tatgacggct ttccgatttt gcgtgactac cgctacctcg taaccggtaa gtatgtacct gcgagcacct ctgtagagtg tagccacatg tttcacgctc gaccgatggc gctgctcgag ctgatctcca tgatagatac gtgcccgttc ctgaaacatg tagatcttag ctggaataac attcacagct tatctgtaat ccagtcgctg ctcaaggtgg tgacacgaca cccttcattg caatctattg acctctctca caatcccatc ccggccgtgg cagggcgttc catcgtgcga ctgatccgca acccgctttc acaggtgaca tgcatcaacg tcggcgacac cggaatcagt gccgacacca ttgggcagat caactccttt ttaaaggaga agctaacccc tgccgcgctg tcgtacagcg tctccaccgg agacatgcaa agccgactca ctcagggtag cggtgatgac gcgctcatga cgtcatcgct cactatggtg agctccttgc agcaaaagcg tagcatcggt agtgggccac ccaagcagcc cgcgaagaaa gaatcgcgcg gcgtgcggct acccccgatt tcacgtccac cagtggctag gcgccatggg aagatgcctt ag
Details for LemaA.01010.a.HZ2.GE43717
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 61
Validated AA Sequence
MGEVETMSLL SHPNIVKLEE TYQDEACLSI VMEYLPGGSX QHRIDRQSVS ELETRRFITQ LLMAVEYIHE KGIVHRDLKP SNCLLSQNDL VVKISDFGLS VFAGSKQCLT TCCGTLHFMA PEILLEKNYG KPVDMWAMGV MAHVMFLGCY PFQARTEAAL TKEICRGYRP REEGAGGLRC PPLLLDFISQ LLLYDPHRRM SAKDALKHPW IKEGLEANRR TSLYPSQAPS ATAMKPSARW RAAVLAVMGV QRLLYLQKIQ RLVRLGYDGF P
Validated NT Sequence
tnggggntnn nntagcagcc ggatcctcga gaagcttggc tgcagaactt gttcgtgctg tttattacgg aaagccgtca taaccgagcc gcaccagccg ctgaattttc tgcaagtaga gcagccgctg cacgcccatg actgcaagta ccgccgctcg ccaccgcgca gatggcttca tcgctgtcgc cgagggtgcc tgtgacgggt agagggatgt ccgccggttg gcttctaagc cttccttgat ccatgggtgc ttgagagcgt ctttggcgct cattcgacgg tgtgggtcgt aaaggagaag ctgactgata aagtccagta gcagtggtgg acatcgcagc ccaccagcac cctcttcccg cggacgatac ccgcggcaga tctcctttgt cagcgccgcc tctgtacggg cctgaaatgg gtaacaacct aaaaacatca cgtgtgccat gacgcccatg gcccacatgt ccaccggctt gccgtagttt ttctcgagca gaatctcggg tgccataaag tgcagcgtgc cacagcacgt cgtcaagcat tgcttactgc cggcaaacac ggagagcccg aagtctgata tctttacgac gaggtcgttt tgagacagta ggcagttgct cggctttaaa tcgcggtgga cgataccttt ttcatgaatg tactcgacag ccatcagcag ctgcgtgatg aagcgccgcg tctccagctc agacacgctt tgccggtcta tccggtgctg aanggaaccg ccagggaggt actccatcac aattgatagg caagcttcgt cttgatacgt ctcctccagc ttgacgatat tgggatgaga aagaaggctc atcgtctcca cttcccccat gacgctccgc agtcccttgg tgccccgccn tganncttgn cgatgccttc accnncgtag atgtccttcg ccctgtgcgc cgagtcncgc tccaganatc gccatnanag cnnnnccgat tttctccatc aagacgtacc tcnactcgac gtncgccggt catcnancnn gnacctgggt ctgagcttnc cagccaccna t
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSTG DVESRYVLME KIGSGSYGDV WSATRRTGAK DIYAVKRIDK RQAGTKGLRS VMGEVETMSL LSHPNIVKLE ETYQDEACLS IVMEYLPGGS LQHRIDRQSV SELETRRFIT QLLMAVEYIH EKGIVHRDLK PSNCLLSQND LVVKISDFGL SVFAGSKQCL TTCCGTLHFM APEILLEKNY GKPVDMWAMG VMAHVMFLGC YPFQARTEAA LTKEICRGYR PREEGAGGLR CPPLLLDFIS QLLLYDPHRR MSAKDALKHP WIKEGLEANR RTSLYPSQAP SATAMKPSAR WRAAVLAVMG VQRLLYLQKI QRLVRLGYDG FP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga ccggcgacgt cgagtcgagg tacgtcttga tggagaaaat cggctctggc tcttatggcg atgtctggag cgcgactcgg cgcacagggg cgaaggacat ctacgcggtg aagcgcatcg acaagcgtca ggcgggcacc aagggactgc ggagcgtcat gggggaagtg gagacgatga gccttctttc tcatcccaat atcgtcaagc tggaggagac gtatcaagac gaagcttgcc tatcaattgt gatggagtac ctccctggcg gttcccttca gcaccggata gaccggcaaa gcgtgtctga gctggagacg cggcgcttca tcacgcagct gctgatggct gtcgagtaca ttcatgaaaa aggtatcgtc caccgcgatt taaagccgag caactgccta ctgtctcaaa acgacctcgt cgtaaagata tcagacttcg ggctctccgt gtttgccggc agtaagcaat gcttgacgac gtgctgtggc acgctgcact ttatggcacc cgagattctg ctcgagaaaa actacggcaa gccggtggac atgtgggcca tgggcgtcat ggcacacgtg atgtttttag gttgttaccc atttcaggcc cgtacagagg cggcgctgac aaaggagatc tgccgcgggt atcgtccgcg ggaagagggt gctggtgggc tgcgatgtcc accactgcta ctggacttta tcagtcagct tctcctttac gacccacacc gtcgaatgag cgccaaagac gctctcaagc acccatggat caaggaaggc ttagaagcca accggcggac atccctctac ccgtcacagg caccctcggc gacagcgatg aagccatctg cgcggtggcg agcggcggta cttgcagtca tgggcgtgca gcggctgctc tacttgcaga aaattcagcg gctggtgcgg ctcggttatg acggctttcc gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa