SSGCID: Target Page

LemaA.00825.a

aspartyl-tRNA synthetase, putative

CENTER ID:	LemaA.00825.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.00825.a.B2.GE42540	soluble domain		27	641

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.21.0895
UniProt:	Q4QCA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRVSLSSCV SCRLRGVAGP TPGVVAAVVV AASSSAVPKR WTSLPNSQVS TQRLCGTSSS ATQGASSIRS PHCTSLSPPS TPSAAPTETA TLTNGAISGG GCGRACLAAH GASIASLRAA FNGSCGESGC VREGDEVTVR ARLERTRGRG KLAFLHLRQP PLESVQAVCE GKELTKQARS ITLESIVDVT GIVRRADTPV QSTTCQGWEL QVTALHVVSQ AATPLPFPYR DTNARLDTRL NHRVMDLRTE KMIAASRLVS ALGQSFRNEL LARDFIEVHT PKLLGAASEG GSNVFRVDYF ERKAYLAQSP QLHKQMMVMG DAMRVFEVGP VFRAENSLTH RHLTEFVGLD GEMVIKDSHT EVLDVLEPVM CAVLAHLTQQ HGNLIDALWK QQQQQGEGLQ TSGGDPQPAT AAEPPRCGSR GHAPRDVCCE VALERMTPLG ITTDASPTTR TPFKIPAQVD PYHARVGGDG SGCRVLRMSF ADASQLLAGC GGPEDMADCT LPVEDFTLPQ ERRLGQLIKE RYGVDLYVID SFPSSARPFY TMPVDPSMPD GPTRSYDMYL RGEEICSGAQ RVHQIELLEQ RLSAKKVDKV SVKDYVDSFR YGAWPHGGFG LGLERIALFF LGLDDIRQVS LFPRDPKRIS P

NT Sequence

atgctgcggg tgtcgctctc gtcgtgcgtg tcatgtcgcc tgcgtggtgt tgcagggccc acgcccggtg tggtggccgc ggtggtcgtc gccgcgtctt cgtcagcggt gcccaagaga tggacatcgc tccctaactc acaggtctcc acgcagcgcc tctgcggtac gagctccagc gctacccaag gtgcttccag catcagatcg cctcattgca cctcattgtc tccaccctct accccatctg ctgccccaac ggagacagcc acgttgacta acggcgccat ctcgggaggc ggctgcggca gagcgtgcct ggctgcacac ggggcctcca ttgcctccct ccgcgctgcc tttaacggca gctgcggtga gagtgggtgc gttcgcgaag gtgacgaggt caccgtgcgg gcccgcttag agcggacgcg cggccgcggc aagcttgcct ttctccacct ccggcagccg ccgctggagt ccgtgcaggc agtgtgcgag ggcaaggagc tgacaaagca ggcgaggagc attacgctag agtctattgt cgatgtcacg ggcatcgtgc gccgcgcgga cacccctgtg cagtcgacta cgtgccaggg gtgggagctg caggtgacgg cgctgcatgt cgtcagtcag gcggccaccc cgctaccgtt tccgtatcgc gacaccaatg caaggctgga cacccgcctc aaccaccgcg ttatggacct ccgcacagaa aaaatgatcg cggcgtcgcg gctcgtgtcg gctctcgggc agagcttccg caacgagctt ctcgcacgcg atttcatcga ggtgcacacg ccgaagctgc ttggggccgc gtcggagggc ggcagcaacg tgttccgggt ggactacttt gagcgcaagg cgtacctggc gcagtcaccg cagctgcaca agcagatgat ggtcatgggc gacgcgatgc gggtgtttga ggtcgggccc gtgtttcgcg ccgagaatag cctgacacat cggcacttga cggagtttgt tggtctcgat ggtgagatgg tgatcaagga cagtcacacg gaggtgctcg acgtgctgga gccagtcatg tgcgccgttc tggcgcacct cacacagcag cacggcaacc ttatcgatgc tctctggaag cagcagcagc agcaagggga agggctgcaa accagcggcg gcgatccgca acctgccacc gctgcggagc cgcctcgctg tggcagtcgt ggtcacgcgc cgcgtgatgt gtgctgcgag gtagcactgg agcgcatgac ccccttgggg atcaccacag acgcctctcc gacgacgcgg acaccgttca aaatccccgc gcaagttgat ccttaccacg ctcgcgtggg cggagatggt agcgggtgcc gcgtgcttcg catgagcttc gcagatgcgt cgcagctgct ggcgggctgc ggcggcccag aggacatggc cgactgcaca ctgcccgttg aggactttac gctgccgcag gagcgccgcc ttggccagct cataaaagag cgctacgggg ttgacctcta cgtgatcgac agctttcctt cctctgccag gcccttttac acgatgccgg tggacccatc aatgccggac ggtccaacgc ggagctacga catgtaccta cgtggggagg agatctgcag cggtgcacag cgtgtccatc agatagagct gctggaacaa cggctgtcgg cgaagaaggt ggacaaggtg agtgtgaagg actacgtcga ctccttccgc tacggcgcgt ggccacacgg cggcttcggc ctggggctcg agcgcattgc cctcttcttc ctcggcctcg acgacatccg gcaggtgtca ttgttcccac gcgacccgaa gcggatcagc ccgtga

Details for LemaA.00825.a.B2.GE42540

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHAV VVAASSSAVP KRWTSLPNSQ VSTQRLCGTS SSATQGASSI RSPHCTSLSP PSTPSAAPTE TATLTNGAIS GGGCGRACLA AHGASIASLR AAFNGSCGES GCVREGDEVT VRARLERTRG RGKLAFLHLR QPPLESVQAV CEGKELTKQA RSITLESIVD VTGIVRRADT PVQSTTCQGW ELQVTALHVV SQAATPLPFP YRDTNARLDT RLNHRVMDLR TEKMIAASRL VSALGQSFRN ELLARDFIEV HTPKLLGAAS EGGSNVFRVD YFERKAYLAQ SPQLHKQMMV MGDAMRVFEV GPVFRAENSL THRHLTEFVG LDGEMVIKDS HTEVLDVLEP VMCAVLAHLT QQHGNLIDAL WKQQQQQGEG LQTSGGDPQP ATAAEPPRCG SRGHAPRDVC CEVALERMTP LGITTDASPT TRTPFKIPAQ VDPYHARVGG DGSGCRVLRM SFADASQLLA GCGGPEDMAD CTLPVEDFTL PQERRLGQLI KERYGVDLYV IDSFPSSARP FYTMPVDPSM PDGPTRSYDM YLRGEEICSG AQRVHQIELL EQRLSAKKVD KVSVKDYVDS FRYGAWPHGG FGLGLERIAL FFLGLDDIRQ VSLFPRDPKR ISP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcggtgg tcgtcgccgc gtcttcgtca gcggtgccca agagatggac atcgctccct aactcacagg tctccacgca gcgcctctgc ggtacgagct ccagcgctac ccaaggtgct tccagcatca gatcgcctca ttgcacctca ttgtctccac cctctacccc atctgctgcc ccaacggaga cagccacgtt gactaacggc gccatctcgg gaggcggctg cggcagagcg tgcctggctg cacacggggc ctccattgcc tccctccgcg ctgcctttaa cggcagctgc ggtgagagtg ggtgcgttcg cgaaggtgac gaggtcaccg tgcgggcccg cttagagcgg acgcgcggcc gcggcaagct tgcctttctc cacctccggc agccgccgct ggagtccgtg caggcagtgt gcgagggcaa ggagctgaca aagcaggcga ggagcattac gctagagtct attgtcgatg tcacgggcat cgtgcgccgc gcggacaccc ctgtgcagtc gactacgtgc caggggtggg agctgcaggt gacggcgctg catgtcgtca gtcaggcggc caccccgcta ccgtttccgt atcgcgacac caatgcaagg ctggacaccc gcctcaacca ccgcgttatg gacctccgca cagaaaaaat gatcgcggcg tcgcggctcg tgtcggctct cgggcagagc ttccgcaacg agcttctcgc acgcgatttc atcgaggtgc acacgccgaa gctgcttggg gccgcgtcgg agggcggcag caacgtgttc cgggtggact actttgagcg caaggcgtac ctggcgcagt caccgcagct gcacaagcag atgatggtca tgggcgacgc gatgcgggtg tttgaggtcg ggcccgtgtt tcgcgccgag aatagcctga cacatcggca cttgacggag tttgttggtc tcgatggtga gatggtgatc aaggacagtc acacggaggt gctcgacgtg ctggagccag tcatgtgcgc cgttctggcg cacctcacac agcagcacgg caaccttatc gatgctctct ggaagcagca gcagcagcaa ggggaagggc tgcaaaccag cggcggcgat ccgcaacctg ccaccgctgc ggagccgcct cgctgtggca gtcgtggtca cgcgccgcgt gatgtgtgct gcgaggtagc actggagcgc atgaccccct tggggatcac cacagacgcc tctccgacga cgcggacacc gttcaaaatc cccgcgcaag ttgatcctta ccacgctcgc gtgggcggag atggtagcgg gtgccgcgtg cttcgcatga gcttcgcaga tgcgtcgcag ctgctggcgg gctgcggcgg cccagaggac atggccgact gcacactgcc cgttgaggac tttacgctgc cgcaggagcg ccgccttggc cagctcataa aagagcgcta cggggttgac ctctacgtga tcgacagctt tccttcctct gccaggccct tttacacgat gccggtggac ccatcaatgc cggacggtcc aacgcggagc tacgacatgt acctacgtgg ggaggagatc tgcagcggtg cacagcgtgt ccatcagata gagctgctgg aacaacggct gtcggcgaag aaggtggaca aggtgagtgt gaaggactac gtcgactcct tccgctacgg cgcgtggcca cacggcggct tcggcctggg gctcgagcgc attgccctct tcttcctcgg cctcgacgac atccggcagg tgtcattgtt cccacgcgac ccgaagcgga tcagcccgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat