SSGCID: Target Page

LemaA.00612.b

lysyl-tRNA synthetase, putative

CENTER ID:	LemaA.00612.b
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.00612.b.B1.GE42539	full length		1	586

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.15.0230
UniProt:	Q4QFF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSEELRKQ IEAIASQIAD IKKTKGTDSD EYKSLVKQMG ALRSQLPQDE KKTKKDKTVE PSYFESRLAM VKEMGLLGAA YPHKYHRQYT IPQYRRKYAP LLTEPDTSLD ETVTIAGRII NKRSSGSKLH FITIQGDMEI VQVISALTEY IDQSKFAEIH SKLKRGDIIG IAGKPSLSKS NEFSLKATEI TLLSTCYHML PDDYFGLSSF EQRFRQRYLD FIVNRDNIKT FIQRANIIKY IRKFFDERDF VEVETPVLNQ IAGGAAARPF VTHHNDLNQT MFLRIAPELY LKELVVGGMD RVYEIGKQFR NEGIDLTHNP EFTSCEAYWA YMDYHDWMTA TEDLLYGLAV ELHGSPIVRY APKDSEGKPL PEATFNFSKP FKRLHIIPEL EKRLKVSFDN VDFESDAGIQ FLMDLCKKHK ADCPPPYTAP RLLDALIAEF LEPECHDPCF ICDHPRVMSP LAKWHRNDPR LTERFELFVN KKELANAYTE LNNPIVQREE FLKQVRNRDK GDDESMEIDE GFVAALEHAL PPTGGWGLGI DRLVMFLTSQ SNIKEVLLFP AMKPEGKNAI SYPPGTMLNG QGVPLL

NT Sequence

atgtcgtcct ccgaagagct ccgtaagcag atagaggcta ttgcctccca gatcgcagac atcaaaaaga cgaagggtac tgatagtgat gagtacaagt cgctggtgaa gcagatgggt gcgctgcgca gccagcttcc gcaggatgaa aagaagacga agaaggacaa gacggtagag ccgtcgtact tcgagtcgcg cctggcgatg gtcaaggaga tgggtcttct tggcgctgcc tacccccaca agtaccaccg tcaatacaca attcctcagt atcgcaggaa gtacgcgcct ttgcttacag agcccgacac atccctcgac gaaacggtga caatcgcggg ccgcatcatc aataagcgta gctccggttc gaagctgcac ttcattacta tccagggcga tatggagatt gtgcaggtga tttctgccct cacggaatac attgaccagt ccaagttcgc tgagatccac tccaagctga agcgcggcga tatcatcggt attgcgggta agccgtccct atccaagagc aacgagtttt ccctcaaggc aaccgagatt acgcttcttt caacttgcta ccacatgctc cccgacgact actttggcct ttcttccttt gagcagcgct tccgtcagcg ctacctcgac tttattgtca accgcgacaa tatcaagacc ttcattcagc gtgcaaacat catcaagtac attcgcaagt ttttcgatga gcgggacttc gtggaggtcg agacgccggt gctcaaccaa atcgctggtg gcgccgcggc gcgtcctttt gtgacgcacc acaacgacct caaccagacc atgttcctgc gtatcgcgcc ggagctctac ctgaaggagc tcgtcgtcgg tggcatggat cgcgtgtacg aaataggcaa gcaattccgt aacgagggca tcgatctgac ccacaacccc gaattcacaa gctgcgaggc gtactgggcg tacatggact accacgattg gatgaccgca acggaggacc ttctgtacgg cctggccgtg gagcttcacg gctcgccaat cgtccgctat gcgccgaagg actcagaggg caagccgctg ccagaggcca ccttcaactt cagcaagccg tttaagcgtc tgcacattat tccggagctg gagaagcgct tgaaggtatc ctttgacaac gttgacttcg agtccgatgc gggcattcag tttctgatgg acctgtgcaa aaagcataag gctgactgcc ctccaccgta cacggcgcct cgtcttcttg atgccctgat cgctgagttc ctggagcccg agtgccacga cccctgcttc atctgcgatc accctcgcgt gatgtcacct ctcgccaagt ggcaccgcaa cgaccctcgc ctgacggagc gctttgagct gttcgtcaat aaaaaggagc ttgctaatgc gtacacggag ctgaacaacc ctatcgtgca acgtgaggaa ttccttaaac aggtgcggaa ccgtgacaag ggtgacgacg aatctatgga gatcgatgag ggcttcgtcg cagcactgga gcacgcgctg ccgcccactg gtggatgggg tcttggcatc gaccgccttg tgatgttcct cacaagtcag tccaacatca aggaagtgct tctgttccct gccatgaagc ctgaaggcaa gaacgcgatt agctaccctc cgggaacgat gctcaatggt cagggcgttc ctctgttgta g

Details for LemaA.00612.b.B1.GE42539

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	86
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS SSEELRKQIE AIASQIADIK KTKGTDSDEY KSLVKQMGAL RSQLPQDEKK TKKDKTVEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLL TEPDTSLDET VTIAGHIINK RSSGSKLHFI TIQGDMEIVQ VISALTEYID QSKFAEIHSK LKRGDIIGIA GKPSLSKSNE FSLKATEITL LSTCYHMLPD DYFGLSSFXX RFRQRYLDFI VXRXNIXXFI QRANIXXYIR XXXXXXXXXX XETXXXXXXX XXAAARPFXX HXXXXXXXXX XXXXXXXXXX XXXXXGXXXX XXXGXXXXNX XXXXTHNXXX TXXEAXXAYM DYHDWMTATX XXXYGLAVEL HGSPIVRYAP KDSEGKPLPE ATFNFSKPFK RLHIIPELEK RLKVSFDNVD FESDAGIQFL MDLCKKHKAD CPPPYTAPRL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFL KQVRNRDKGD DESMEIDEGF VAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTML

Validated NT Sequence

accatggctc accaccacca ccaccatatg tcgtcctccg aagagctccg taagcagata gaggctattg cctcccagat cgcagacatc aaaaagacga agggtactga tagtgatgag tacaagtcgc tggtgaagca gatgggtgcg ctgcgcagcc agcttccgca ggatgaaaag aagacgaaga aggacaagac ggtagagccg tcgtacttcg agtcgcgcct ggcgatggtc aaggagatgg gtcttcttgg cgctgcctac ccccacaagt accaccgtca atacacaatt cctcagtatc gcaggaagta cgcgcctttg cttacagagc ccgacacatc cctcgacgaa acggtgacaa tcgcgggcca catcatcaat aagcgtagct ccggttcgaa gctgcacttc attactatcc agggcgatat ggagattgtg caggtgattt ctgccctcac ggaatacatt gaccagtcca agttcgctga gatccactcc aagctgaagc gcggcgatat catcggtatt gcgggtaagc cgtccctatc caagagcaac gagttttccc tcaaggcaac cgagattacg cttctttcaa cttgctacca catgctcccc gacgactact ttggcctttc ttcctttgnn nagcgcttcc gtcagcgcta cctcgacttt attgtnnnnc gcgncaatat cnnnnccttc attcagcgtg caaacatcnn nnngtacatt cgcannnnnn nnnntnnnnn ngncnnnnnn nnnnnngaga cnnnnnnnnn nnnnnaaatn nnnnntngcg ccgcggcgcg tccttttnnn ncgcaccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg catnnnnnnn nnnnnnnnnn nnggnnnnnn nnnnngtaac gannnnnnnn nnnngaccca caaccnnnnn nncacaannn nngaggcntn nngggcgtac atggactacc acgattggat gaccgcaacg gnnnnnnntn nntacggcct ggccgtggag cttcacggct cgccaatcgt ccgctatgcg ccgaaggact cagagggcaa gccgctgcca gaggccacct tcaacttcag caagccgttt aagcgtctgc acattattcc ggagctggag aagcgcttga aggtatcctt tgacaacgtt gacttcgagt ccgatgcggg cattcagttt ctgatggacc tgtgcaaaaa gcataaggct gactgccctc caccgtacac ggcgcctcgt cttcttgatg ccctgatcgc tgagttcctg gagcccgagt gccacgaccc ctgcttcatc tgcgatcacc ctcgcgtgat gtcacctctc gccaagtggc accgcaacga ccctcgcctg acggagcgct ttgagctgtt cgtcaataaa aaggagcttg ctaatgcgta cacggagctg aacaacccta tcgtgcaacg tgaggaattc cttaaacagg tgcggaaccg tgacaagggt gacgacgaat ctatggagat cgatgagggc ttcgtcgcag cactggagca cgcgctgccg cccactggtg gatggggtct tggcatcgac cgccttgtga tgttcctcac aagtcagtcc aacatcaagg aagtgcttct gttccctgcc atgaagcctg aaggcaagaa cgcgattagc taccctccgg gaacgatgct ca

Expected Protein Sequence

MAHHHHHHMS SSEELRKQIE AIASQIADIK KTKGTDSDEY KSLVKQMGAL RSQLPQDEKK TKKDKTVEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLL TEPDTSLDET VTIAGRIINK RSSGSKLHFI TIQGDMEIVQ VISALTEYID QSKFAEIHSK LKRGDIIGIA GKPSLSKSNE FSLKATEITL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDFI VNRDNIKTFI QRANIIKYIR KFFDERDFVE VETPVLNQIA GGAAARPFVT HHNDLNQTMF LRIAPELYLK ELVVGGMDRV YEIGKQFRNE GIDLTHNPEF TSCEAYWAYM DYHDWMTATE DLLYGLAVEL HGSPIVRYAP KDSEGKPLPE ATFNFSKPFK RLHIIPELEK RLKVSFDNVD FESDAGIQFL MDLCKKHKAD CPPPYTAPRL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFL KQVRNRDKGD DESMEIDEGF VAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTMLNGQG VPLL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt cctccgaaga gctccgtaag cagatagagg ctattgcctc ccagatcgca gacatcaaaa agacgaaggg tactgatagt gatgagtaca agtcgctggt gaagcagatg ggtgcgctgc gcagccagct tccgcaggat gaaaagaaga cgaagaagga caagacggta gagccgtcgt acttcgagtc gcgcctggcg atggtcaagg agatgggtct tcttggcgct gcctaccccc acaagtacca ccgtcaatac acaattcctc agtatcgcag gaagtacgcg cctttgctta cagagcccga cacatccctc gacgaaacgg tgacaatcgc gggccgcatc atcaataagc gtagctccgg ttcgaagctg cacttcatta ctatccaggg cgatatggag attgtgcagg tgatttctgc cctcacggaa tacattgacc agtccaagtt cgctgagatc cactccaagc tgaagcgcgg cgatatcatc ggtattgcgg gtaagccgtc cctatccaag agcaacgagt tttccctcaa ggcaaccgag attacgcttc tttcaacttg ctaccacatg ctccccgacg actactttgg cctttcttcc tttgagcagc gcttccgtca gcgctacctc gactttattg tcaaccgcga caatatcaag accttcattc agcgtgcaaa catcatcaag tacattcgca agtttttcga tgagcgggac ttcgtggagg tcgagacgcc ggtgctcaac caaatcgctg gtggcgccgc ggcgcgtcct tttgtgacgc accacaacga cctcaaccag accatgttcc tgcgtatcgc gccggagctc tacctgaagg agctcgtcgt cggtggcatg gatcgcgtgt acgaaatagg caagcaattc cgtaacgagg gcatcgatct gacccacaac cccgaattca caagctgcga ggcgtactgg gcgtacatgg actaccacga ttggatgacc gcaacggagg accttctgta cggcctggcc gtggagcttc acggctcgcc aatcgtccgc tatgcgccga aggactcaga gggcaagccg ctgccagagg ccaccttcaa cttcagcaag ccgtttaagc gtctgcacat tattccggag ctggagaagc gcttgaaggt atcctttgac aacgttgact tcgagtccga tgcgggcatt cagtttctga tggacctgtg caaaaagcat aaggctgact gccctccacc gtacacggcg cctcgtcttc ttgatgccct gatcgctgag ttcctggagc ccgagtgcca cgacccctgc ttcatctgcg atcaccctcg cgtgatgtca cctctcgcca agtggcaccg caacgaccct cgcctgacgg agcgctttga gctgttcgtc aataaaaagg agcttgctaa tgcgtacacg gagctgaaca accctatcgt gcaacgtgag gaattcctta aacaggtgcg gaaccgtgac aagggtgacg acgaatctat ggagatcgat gagggcttcg tcgcagcact ggagcacgcg ctgccgccca ctggtggatg gggtcttggc atcgaccgcc ttgtgatgtt cctcacaagt cagtccaaca tcaaggaagt gcttctgttc cctgccatga agcctgaagg caagaacgcg attagctacc ctccgggaac gatgctcaat ggtcagggcg ttcctctgtt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat