LemaA.00612.a
aspartyl-tRNA synthetase, putative
| CENTER ID: | LemaA.00612.a |
| ORGANISM: | Leishmania major Friedlin |
| ASSOCIATED DISEASE: | Cutaneous leishmaniasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.00612.a.B1.GE42538 | full length | 1 | 550 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.00612.a.B1.PW38547 | full length | 1 | 550 |
Structures
6OD8
DEPOSITED: 3/26/2019
DETERMINATION: XRay
CLONE: LemaA.00612.a.B1.GE42538
PROTEIN: LemaA.00612.a.B1.PW38547
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TriTrypDB:LmjF.30.0460 |
| UniProt: | Q4Q7R2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSANHADAGA PAVAKKMSDK EARKAARLAE EKARADEKAA LVEKYKAVFG AAPMVQSTTY KSRTHIPVSE LSRPELVDKT VLIRARVSTT RKKGKMAFMV LRDGSDSVQA MAAVEGDVPK EMIDFMGQIA TESIVDVEAT VCKVEQPITS TSHSDIELKV KKIHTVTESL RTLPFTLEDA SRKESAEGAK VNLDTRLNSR WMDLRTLASG AIFRLQSRVC QYFRQFLIDK DFCEIHSPKI INAPSEGGAN VFKLEYFNRF AYLAQSPQLY KQMVLQGDVP RVFEVGPVFR SENSNTHRHL TEFVGLDVEM RIDEHYYEVL DVAESLFNYI FERLATHTKE LKNVCQQYPF EPLVWKLTPE RIKELGVGVI SEGVVPTDKF QARVHNMDSR MLRINYMHCI ELLNTVLDEK MAPTDDINTT NEKLLGKLVK ERYGTDFFIS DRFPSSARPF YTMECKDDVR FTNSYDMFIR GEEISSGAQR IHDPDLLLAR AKMLNVDLTP IKEYVDSFRL GAWPHGGFGI GLERVVMLYL GLSNVRLASL FPRDPQRTTP
NT Sequence
atgagcgcaa accacgccga tgccggcgca ccagccgtgg cgaagaagat gagcgacaaa gaggcgcgta aggcggcgcg cctggcggag gagaaggccc gcgcggatga aaaggcggcc cttgtggaga agtacaaggc cgtgtttggt gccgcaccaa tggtgcagtc gacgacgtac aagtcgcgca cgcacatccc ggtctcggag ctgtcgcggc cggagttggt ggacaagacg gtgctgatcc gtgcccgcgt gtcgacgacg cgcaagaagg gcaagatggc gttcatggtg ctgcgcgacg ggagcgattc ggtgcaggcg atggctgccg tggaaggcga tgtgccgaag gagatgatcg acttcatggg gcagatcgcg acagagtcga ttgttgatgt ggaggcgaca gtttgcaagg tggagcagcc catcacgtcg acgtcgcact cggacatcga gctgaaggtg aagaagatcc acacggtgac ggagtcgctg cgcacgctgc cgttcacgct agaggacgcg agccgcaagg agtcggccga gggtgcgaag gtgaacctcg acacgcgcct gaatagccgc tggatggacc tgcgcacact agcgtccggc gcgatcttcc gccttcagtc gcgcgtgtgc cagtacttcc gccagtttct tatcgacaag gacttctgtg agatccactc gcccaagatc atcaacgcgc cgagcgaggg tggcgccaac gtgttcaagc tggagtactt caaccgcttc gcgtaccttg cccagtcgcc acaactgtac aagcagatgg tgctgcaggg cgatgtgccg cgcgtgttcg aggtgggacc ggtgttccgc tcagagaaca gcaacacaca ccgccacctg acggagtttg ttgggttgga cgtggagatg cgcatcgatg agcactacta cgaggtgctg gatgtggcgg agagcctatt caactacatt ttcgagcgcc ttgccactca cacaaaggag ctgaagaacg tgtgccagca gtaccccttc gagcctctcg tgtggaagct cacaccggag aggataaagg agctcggcgt tggcgtcatc tcggagggcg tggtgccgac agacaagttt caggcacgcg tgcacaacat ggatagccgt atgctgcgta tcaactacat gcactgcatt gagctgttga acactgtgct ggacgagaag atggcgccga cggatgacat caacacgacg aacgagaagc tgctcggcaa gcttgtgaag gagcgctacg gcacagactt cttcatctcg gaccgctttc cgtcctcggc gcgcccgttc tacacgatgg agtgcaagga cgacgtgcgc ttcacgaact cgtacgatat gttcatccgc ggtgaggaga tctccagcgg agcgcagcgc atccacgacc ccgatctgct gctggcacgc gccaagatgc tgaacgtgga tctcacaccg atcaaggagt acgtcgactc cttccgtctc ggtgcgtggc cgcacggcgg cttcggcatt gggctggagc gcgtggtgat gctgtacctt ggactgagca acgtgcgcct tgcttcgctc tttccgcgtg acccgcagcg cacgacgcca tag
Details for LemaA.00612.a.B1.GE42538
| HARVESTED ON: | 7/3/2018 |
| SEQUENCED ON: | 7/10/2018 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 63kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS ANHADAGAPA VAKKMSDKEA RKAARLAEEK ARADEKAALV EKYKAVFGAA PMVQSTTYKS RTHIPVSELS RPELVDKTVL IRARVSTTRK KGKMAFMVLR DGSDSVQAMA AVEGDVPKEM IDFMGQIATE SIVDVEATVC KVEQPITSTS HSDIELKVKK IHTVTESLRT LPFTLEDASR KESAEGAKVN LDTRLNSRWM DLRTLASGAI FRLQSRVCQY FRQFLIDKDF CXXHSPKIIN APSEGGANVF KLEYFNRXXX XXQXXXXXKX MVLQGDVPRV FEVGPVXXSE NSNTHRHLTE FVXXDVEMRI DEHYYEVLDV AESLFNYIFE RLATHTKELK NVCQQYPFEP LVWKLTPERI KELGVGVISE GVVPTDKFQA RVHNMDSRML RINYMHCIEL LNTVLDEKMA PTDDINTTNE KLLGKLVKER YGTDFFISDR FPSSARPFYT MECKDDVRFT NSYDMFIRGE EISSGAQRIH DPDLLLARAK MLNVDLTPIK EYVDSFRLGA WPHGGFGIGL ERVVMLYLGL SNVRLASLF
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gcaaaccacg ccgatgccgg cgcaccagcc gtggcgaaga agatgagcga caaagaggcg cgtaaggcgg cgcgcctggc ggaggagaag gcccgcgcgg atgaaaaggc ggcccttgtg gagaagtaca aggccgtgtt tggtgccgca ccaatggtgc agtcgacgac gtacaagtcg cgcacgcaca tcccggtctc ggagctgtcg cggccggagt tggtggacaa gacggtgctg atccgtgccc gcgtgtcgac gacgcgcaag aagggcaaga tggcgttcat ggtgctgcgc gacgggagcg attcggtgca ggcgatggct gccgtggaag gcgatgtgcc gaaggagatg atcgacttca tggggcagat cgcgacagag tcgattgttg atgtggaggc gacagtttgc aaggtggagc agcccatcac gtcgacgtcg cactcggaca tcgagctgaa ggtgaagaag atccacacgg tgacggagtc gctgcgcacg ctgccgttca cgctagagga cgcgagccgc aaggagtcgg ccgagggtgc gaaggtgaac ctcgacacgc gcctgaatag ccgctggatg gacctgcgca cactagcgtc cggcgcgatc ttccgccttc agtcgcgcgt gtgccagtac ttccgccagt ttcttatcga caaggacttc tgtganntcc actcgcccaa gatcatcaac gcgccgagcg agggtggcgc caacgtgttc aagctggagt acttcaaccg nnnnnnnnac cnnncncagt nnnnacnnnn gnnnaagnag atggtgctgc agggcgatgt gccgcgcgtg ttcgaggtgg gaccggtnnn nnnntcagag aacagcaaca cacaccgcca cctgacggag tttgttnnnn tggacgtgga gatgcgcatc gatgagcact actacgaggt gctggatgtg gcggagagcc tattcaacta cattttcgag cgccttgcca ctcacacaaa ggagctgaag aacgtgtgcc agcagtaccc cttcgagcct ctcgtgtgga agctcacacc ggagaggata aaggagctcg gcgttggcgt catctcggag ggcgtggtgc cgacagacaa gtttcaggca cgcgtgcaca acatggatag ccgtatgctg cgtatcaact acatgcactg cattgagctg ttgaacactg tgctggacga gaagatggcg ccgacggatg acatcaacac gacgaacgag aagctgctcg gcaagcttgt gaaggagcgc tacggcacag acttcttcat ctcggaccgc tttccgtcct cggcgcgccc gttctacacg atggagtgca aggacgacgt gcgcttcacg aactcgtacg atatgttcat ccgcggtgag gagatctcca gcggagcgca gcgcatccac gaccccgatc tgctgctggc acgcgccaag atgctgaacg tggatctcac accgatcaag gagtacgtcg actccttccg tctcggtgcg tggccgcacg gcggcttcgg cattgggctg gagcgcgtgg tgatgctgta ccttggactg agcaacgtgc gccttgcttc gctctttcc
Expected Protein Sequence
MAHHHHHHMS ANHADAGAPA VAKKMSDKEA RKAARLAEEK ARADEKAALV EKYKAVFGAA PMVQSTTYKS RTHIPVSELS RPELVDKTVL IRARVSTTRK KGKMAFMVLR DGSDSVQAMA AVEGDVPKEM IDFMGQIATE SIVDVEATVC KVEQPITSTS HSDIELKVKK IHTVTESLRT LPFTLEDASR KESAEGAKVN LDTRLNSRWM DLRTLASGAI FRLQSRVCQY FRQFLIDKDF CEIHSPKIIN APSEGGANVF KLEYFNRFAY LAQSPQLYKQ MVLQGDVPRV FEVGPVFRSE NSNTHRHLTE FVGLDVEMRI DEHYYEVLDV AESLFNYIFE RLATHTKELK NVCQQYPFEP LVWKLTPERI KELGVGVISE GVVPTDKFQA RVHNMDSRML RINYMHCIEL LNTVLDEKMA PTDDINTTNE KLLGKLVKER YGTDFFISDR FPSSARPFYT MECKDDVRFT NSYDMFIRGE EISSGAQRIH DPDLLLARAK MLNVDLTPIK EYVDSFRLGA WPHGGFGIGL ERVVMLYLGL SNVRLASLFP RDPQRTTP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg caaaccacgc cgatgccggc gcaccagccg tggcgaagaa gatgagcgac aaagaggcgc gtaaggcggc gcgcctggcg gaggagaagg cccgcgcgga tgaaaaggcg gcccttgtgg agaagtacaa ggccgtgttt ggtgccgcac caatggtgca gtcgacgacg tacaagtcgc gcacgcacat cccggtctcg gagctgtcgc ggccggagtt ggtggacaag acggtgctga tccgtgcccg cgtgtcgacg acgcgcaaga agggcaagat ggcgttcatg gtgctgcgcg acgggagcga ttcggtgcag gcgatggctg ccgtggaagg cgatgtgccg aaggagatga tcgacttcat ggggcagatc gcgacagagt cgattgttga tgtggaggcg acagtttgca aggtggagca gcccatcacg tcgacgtcgc actcggacat cgagctgaag gtgaagaaga tccacacggt gacggagtcg ctgcgcacgc tgccgttcac gctagaggac gcgagccgca aggagtcggc cgagggtgcg aaggtgaacc tcgacacgcg cctgaatagc cgctggatgg acctgcgcac actagcgtcc ggcgcgatct tccgccttca gtcgcgcgtg tgccagtact tccgccagtt tcttatcgac aaggacttct gtgagatcca ctcgcccaag atcatcaacg cgccgagcga gggtggcgcc aacgtgttca agctggagta cttcaaccgc ttcgcgtacc ttgcccagtc gccacaactg tacaagcaga tggtgctgca gggcgatgtg ccgcgcgtgt tcgaggtggg accggtgttc cgctcagaga acagcaacac acaccgccac ctgacggagt ttgttgggtt ggacgtggag atgcgcatcg atgagcacta ctacgaggtg ctggatgtgg cggagagcct attcaactac attttcgagc gccttgccac tcacacaaag gagctgaaga acgtgtgcca gcagtacccc ttcgagcctc tcgtgtggaa gctcacaccg gagaggataa aggagctcgg cgttggcgtc atctcggagg gcgtggtgcc gacagacaag tttcaggcac gcgtgcacaa catggatagc cgtatgctgc gtatcaacta catgcactgc attgagctgt tgaacactgt gctggacgag aagatggcgc cgacggatga catcaacacg acgaacgaga agctgctcgg caagcttgtg aaggagcgct acggcacaga cttcttcatc tcggaccgct ttccgtcctc ggcgcgcccg ttctacacga tggagtgcaa ggacgacgtg cgcttcacga actcgtacga tatgttcatc cgcggtgagg agatctccag cggagcgcag cgcatccacg accccgatct gctgctggca cgcgccaaga tgctgaacgt ggatctcaca ccgatcaagg agtacgtcga ctccttccgt ctcggtgcgt ggccgcacgg cggcttcggc attgggctgg agcgcgtggt gatgctgtac cttggactga gcaacgtgcg ccttgcttcg ctctttccgc gtgacccgca gcgcacgacg ccatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LemaA.00612.a.B1.PW38547
| PURIFICATION DATe: | 10/15/2018 |
| CONCENTRATION: | 43.59mg/ml |
| OBSERVED MW: | 63kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 8, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 210µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ANHADAGAPA VAKKMSDKEA RKAARLAEEK ARADEKAALV EKYKAVFGAA PMVQSTTYKS RTHIPVSELS RPELVDKTVL IRARVSTTRK KGKMAFMVLR DGSDSVQAMA AVEGDVPKEM IDFMGQIATE SIVDVEATVC KVEQPITSTS HSDIELKVKK IHTVTESLRT LPFTLEDASR KESAEGAKVN LDTRLNSRWM DLRTLASGAI FRLQSRVCQY FRQFLIDKDF CXXHSPKIIN APSEGGANVF KLEYFNRXXX XXQXXXXXKX MVLQGDVPRV FEVGPVXXSE NSNTHRHLTE FVXXDVEMRI DEHYYEVLDV AESLFNYIFE RLATHTKELK NVCQQYPFEP LVWKLTPERI KELGVGVISE GVVPTDKFQA RVHNMDSRML RINYMHCIEL LNTVLDEKMA PTDDINTTNE KLLGKLVKER YGTDFFISDR FPSSARPFYT MECKDDVRFT NSYDMFIRGE EISSGAQRIH DPDLLLARAK MLNVDLTPIK EYVDSFRLGA WPHGGFGIGL ERVVMLYLGL SNVRLASLF
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gcaaaccacg ccgatgccgg cgcaccagcc gtggcgaaga agatgagcga caaagaggcg cgtaaggcgg cgcgcctggc ggaggagaag gcccgcgcgg atgaaaaggc ggcccttgtg gagaagtaca aggccgtgtt tggtgccgca ccaatggtgc agtcgacgac gtacaagtcg cgcacgcaca tcccggtctc ggagctgtcg cggccggagt tggtggacaa gacggtgctg atccgtgccc gcgtgtcgac gacgcgcaag aagggcaaga tggcgttcat ggtgctgcgc gacgggagcg attcggtgca ggcgatggct gccgtggaag gcgatgtgcc gaaggagatg atcgacttca tggggcagat cgcgacagag tcgattgttg atgtggaggc gacagtttgc aaggtggagc agcccatcac gtcgacgtcg cactcggaca tcgagctgaa ggtgaagaag atccacacgg tgacggagtc gctgcgcacg ctgccgttca cgctagagga cgcgagccgc aaggagtcgg ccgagggtgc gaaggtgaac ctcgacacgc gcctgaatag ccgctggatg gacctgcgca cactagcgtc cggcgcgatc ttccgccttc agtcgcgcgt gtgccagtac ttccgccagt ttcttatcga caaggacttc tgtganntcc actcgcccaa gatcatcaac gcgccgagcg agggtggcgc caacgtgttc aagctggagt acttcaaccg nnnnnnnnac cnnncncagt nnnnacnnnn gnnnaagnag atggtgctgc agggcgatgt gccgcgcgtg ttcgaggtgg gaccggtnnn nnnntcagag aacagcaaca cacaccgcca cctgacggag tttgttnnnn tggacgtgga gatgcgcatc gatgagcact actacgaggt gctggatgtg gcggagagcc tattcaacta cattttcgag cgccttgcca ctcacacaaa ggagctgaag aacgtgtgcc agcagtaccc cttcgagcct ctcgtgtgga agctcacacc ggagaggata aaggagctcg gcgttggcgt catctcggag ggcgtggtgc cgacagacaa gtttcaggca cgcgtgcaca acatggatag ccgtatgctg cgtatcaact acatgcactg cattgagctg ttgaacactg tgctggacga gaagatggcg ccgacggatg acatcaacac gacgaacgag aagctgctcg gcaagcttgt gaaggagcgc tacggcacag acttcttcat ctcggaccgc tttccgtcct cggcgcgccc gttctacacg atggagtgca aggacgacgt gcgcttcacg aactcgtacg atatgttcat ccgcggtgag gagatctcca gcggagcgca gcgcatccac gaccccgatc tgctgctggc acgcgccaag atgctgaacg tggatctcac accgatcaag gagtacgtcg actccttccg tctcggtgcg tggccgcacg gcggcttcgg cattgggctg gagcgcgtgg tgatgctgta ccttggactg agcaacgtgc gccttgcttc gctctttcc
Expressed Protein Sequence
MAHHHHHHMS ANHADAGAPA VAKKMSDKEA RKAARLAEEK ARADEKAALV EKYKAVFGAA PMVQSTTYKS RTHIPVSELS RPELVDKTVL IRARVSTTRK KGKMAFMVLR DGSDSVQAMA AVEGDVPKEM IDFMGQIATE SIVDVEATVC KVEQPITSTS HSDIELKVKK IHTVTESLRT LPFTLEDASR KESAEGAKVN LDTRLNSRWM DLRTLASGAI FRLQSRVCQY FRQFLIDKDF CEIHSPKIIN APSEGGANVF KLEYFNRFAY LAQSPQLYKQ MVLQGDVPRV FEVGPVFRSE NSNTHRHLTE FVGLDVEMRI DEHYYEVLDV AESLFNYIFE RLATHTKELK NVCQQYPFEP LVWKLTPERI KELGVGVISE GVVPTDKFQA RVHNMDSRML RINYMHCIEL LNTVLDEKMA PTDDINTTNE KLLGKLVKER YGTDFFISDR FPSSARPFYT MECKDDVRFT NSYDMFIRGE EISSGAQRIH DPDLLLARAK MLNVDLTPIK EYVDSFRLGA WPHGGFGIGL ERVVMLYLGL SNVRLASLFP RDPQRTTP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CAAACCACGC CGATGCCGGC GCACCAGCCG TGGCGAAGAA GATGAGCGAC AAAGAGGCGC GTAAGGCGGC GCGCCTGGCG GAGGAGAAGG CCCGCGCGGA TGAAAAGGCG GCCCTTGTGG AGAAGTACAA GGCCGTGTTT GGTGCCGCAC CAATGGTGCA GTCGACGACG TACAAGTCGC GCACGCACAT CCCGGTCTCG GAGCTGTCGC GGCCGGAGTT GGTGGACAAG ACGGTGCTGA TCCGTGCCCG CGTGTCGACG ACGCGCAAGA AGGGCAAGAT GGCGTTCATG GTGCTGCGCG ACGGGAGCGA TTCGGTGCAG GCGATGGCTG CCGTGGAAGG CGATGTGCCG AAGGAGATGA TCGACTTCAT GGGGCAGATC GCGACAGAGT CGATTGTTGA TGTGGAGGCG ACAGTTTGCA AGGTGGAGCA GCCCATCACG TCGACGTCGC ACTCGGACAT CGAGCTGAAG GTGAAGAAGA TCCACACGGT GACGGAGTCG CTGCGCACGC TGCCGTTCAC GCTAGAGGAC GCGAGCCGCA AGGAGTCGGC CGAGGGTGCG AAGGTGAACC TCGACACGCG CCTGAATAGC CGCTGGATGG ACCTGCGCAC ACTAGCGTCC GGCGCGATCT TCCGCCTTCA GTCGCGCGTG TGCCAGTACT TCCGCCAGTT TCTTATCGAC AAGGACTTCT GTGAGATCCA CTCGCCCAAG ATCATCAACG CGCCGAGCGA GGGTGGCGCC AACGTGTTCA AGCTGGAGTA CTTCAACCGC TTCGCGTACC TTGCCCAGTC GCCACAACTG TACAAGCAGA TGGTGCTGCA GGGCGATGTG CCGCGCGTGT TCGAGGTGGG ACCGGTGTTC CGCTCAGAGA ACAGCAACAC ACACCGCCAC CTGACGGAGT TTGTTGGGTT GGACGTGGAG ATGCGCATCG ATGAGCACTA CTACGAGGTG CTGGATGTGG CGGAGAGCCT ATTCAACTAC ATTTTCGAGC GCCTTGCCAC TCACACAAAG GAGCTGAAGA ACGTGTGCCA GCAGTACCCC TTCGAGCCTC TCGTGTGGAA GCTCACACCG GAGAGGATAA AGGAGCTCGG CGTTGGCGTC ATCTCGGAGG GCGTGGTGCC GACAGACAAG TTTCAGGCAC GCGTGCACAA CATGGATAGC CGTATGCTGC GTATCAACTA CATGCACTGC ATTGAGCTGT TGAACACTGT GCTGGACGAG AAGATGGCGC CGACGGATGA CATCAACACG ACGAACGAGA AGCTGCTCGG CAAGCTTGTG AAGGAGCGCT ACGGCACAGA CTTCTTCATC TCGGACCGCT TTCCGTCCTC GGCGCGCCCG TTCTACACGA TGGAGTGCAA GGACGACGTG CGCTTCACGA ACTCGTACGA TATGTTCATC CGCGGTGAGG AGATCTCCAG CGGAGCGCAG CGCATCCACG ACCCCGATCT GCTGCTGGCA CGCGCCAAGA TGCTGAACGT GGATCTCACA CCGATCAAGG AGTACGTCGA CTCCTTCCGT CTCGGTGCGT GGCCGCACGG CGGCTTCGGC ATTGGGCTGG AGCGCGTGGT GATGCTGTAC CTTGGACTGA GCAACGTGCG CCTTGCTTCG CTCTTTCCGC GTGACCCGCA GCGCACGACG CCATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT