SSGCID: Target Page

LemaA.00600.a

tryptophanyl-tRNA synthetase, putative

CENTER ID:	LemaA.00600.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.00600.a.B1.GE42537	full length		1	480

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.29.0060
UniProt:	E9ADK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMSPFPPLPV YLSGLLTLHV PTRCLHFLVL TPMKTRVGLH RCCASYLRLL SQDRLQLHNP IEFIGALHFF TSLCSPLGVA DFLRMEDPAA QEVVVTPWTV EGDVNYDKLI KNFGCQAIDD KLLERMERLT GKKPHHFLRR GIFFSHRDLN LILDAYEKGQ PFYLYTGRGP SSESMHVGHL IPFMFTKWLQ DTFHVPLVIQ LTDDEKFFFK DLTMEEIDKM TTENLKDIIA FGFDPKLTFI FRDFEYVGQM YRIVARIEKA YTASQVRGCF GFKMEDNCGR WMFPAIQAAP SFSAAFPHIF PLQKGNVFCM IPQAIDQDPY FRLTRDVAPR MGYLKPAVIH SKFFPGLSGS KGKMSSSTGA AVFLTDTPKM IKDKINKHAF SGGGADKKEQ LLLGGNTNVD VPVQWLRFFL EDDEELKRLQ KDYMLGRIMT GDVKKVLIRQ ITSIVTAHQE SRKKVTEADV ELFTAVRVMG PAKEEAESRK

NT Sequence

atgatgtccc cctttccccc gctgcctgtc tatctctctg gcctgctcac ccttcatgtt cctactcgct gccttcactt ccttgtgctt acaccaatga aaacccgggt tggactgcat cgctgctgcg catcttacct gcgcttgctc agccaagatc gactgcagct ccacaaccca atcgaattca ttggcgcact ccattttttc acctcgctgt gctctccgtt gggtgttgcc gacttcttaa gaatggagga ccccgccgcg caggaggttg tcgtgacccc gtggacggtc gagggtgacg tgaactatga caagctcatc aaaaatttcg ggtgccaggc gatcgacgac aagctgctgg agcggatgga gcggctgacc gggaagaaac cccatcactt tctgcgccgc ggcatcttct tctctcatcg tgatttgaac cttatcctcg atgcgtacga gaaggggcag cctttctacc tgtacactgg ccgtggtccc agcagcgagt cgatgcacgt tggccacctg atacctttta tgttcaccaa gtggctgcaa gacacgttcc atgtgccgct ggtcattcag cttaccgatg acgaaaagtt cttcttcaag gacttgacga tggaggagat cgataaaatg accacggaaa acttgaagga catcattgcc tttggttttg atccgaagct gacgttcatt ttcagggact ttgagtacgt cggacagatg tatcgcattg tcgcacgcat cgagaaggcg tacacggcta gccaggtgcg gggctgcttc ggcttcaaga tggaggacaa ctgcggccgc tggatgttcc ctgcgattca ggccgcaccc tctttctcgg cggcgtttcc acacattttt ccgctgcaga agggcaacgt cttctgcatg ataccgcaag cgatcgacca agacccgtac ttccgcctta cgcgcgacgt ggcgccgcgc atgggctatt tgaaaccagc cgtcattcac tccaagttct tcccgggtct tagtgggtcg aaaggcaaga tgagctcatc caccggcgct gccgtctttc tgacggacac cccaaagatg atcaaagaca agatcaacaa gcacgccttc agcggcggtg gcgcagataa gaaggagcag ctgcttcttg gtggtaacac caacgtcgac gtacctgtgc agtggctccg ctttttcctc gaggatgacg aggagctgaa gcgcctccag aaggactaca tgcttggccg catcatgacc ggtgacgtga agaaagtcct catccgacaa atcaccagca tcgtgacggc acaccaggag tcgcggaaaa aggtaacgga ggccgatgtg gagctcttca ctgccgtccg cgtgatggga cctgcgaaag aggaggcgga gtcaaggaag taa

Details for LemaA.00600.a.B1.GE42537

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMM SPFPPLPVYL SGLLTLHVPT RCLHFLVLTP MKTRVGLHRC CASYLRLLSQ DRLQLHNPIE FIGALHFFTS LCSPLGVADF LRMEDPAAQE VVVTPWTVEG DVNYDKLIKN FGCQAIDDKL LERMERLTGK KPHHFLRRGI FFSHRDLNLI LDAYEKGQPF YLYTGRGPSS ESMHVGHLIP FMFTKWLQDT FHVPLVIQLT DDEKFFFKDL TMEEIDKMTT ENLKDIIAFG FDPKLTFIFR DFEYVGQMYR IVARIEKAYT ASQVRGCFGF KMEDNCGRWM FPAIQAAPSF SAAFPHIFPL QKGNVFCMIP QAIDQDPYFR LTRDVAPRMG YLKPAVIHSK FFPGLSGSKG KMSSSTGAAV FLTDTPKMIK DKINKHAFSG GGADKKEQLL LGGNTNVDVP VQWLRFFLED DEELKRLQKD YMLGRIMTGD VKKVLIRQIT SIVTAHQESR KKVTEADVEL FTAVRVMGPA

Validated NT Sequence

accatggctc accaccacca ccaccatatg atgtccccct ttcccccgct gcctgtctat ctctctggcc tgctcaccct tcatgttcct actcgctgcc ttcacttcct tgtgcttaca ccaatgaaaa cccgggttgg actgcatcgc tgctgcgcat cttacctgcg cttgctcagc caagatcgac tgcagctcca caacccaatc gaattcattg gcgcactcca ttttttcacc tcgctgtgct ctccgttggg tgttgccgac ttcttaagaa tggaggaccc cgccgcgcag gaggttgtcg tgaccccgtg gacggtcgag ggtgacgtga actatgacaa gctcatcaaa aatttcgggt gccaggcgat cgacgacaag ctgctggagc ggatggagcg gctgaccggg aagaaacccc atcactttct gcgccgcggc atcttcttct ctcatcgtga tttgaacctt atcctcgatg cgtacgagaa ggggcagcct ttctacctgt acactggccg tggtcccagc agcgagtcga tgcacgttgg ccacctgata ccttttatgt tcaccaagtg gctgcaagac acgttccatg tgccgctggt cattcagctt accgatgacg aaaagttctt cttcaaggac ttgacgatgg aggagatcga taaaatgacc acggaaaact tgaaggacat cattgccttt ggttttgatc cgaagctgac gttcattttc agggactttg agtacgtcgg acagatgtat cgcattgtcg cacgcatcga gaaggcgtac acggctagcc aggtgcgggg ctgcttcggc ttcaagatgg aggacaactg cggccgctgg atgttccctg cgattcaggc cgcaccctct ttctcggcgg cgtttccaca catttttccg ctgcagaagg gcaacgtctt ctgcatgata ccgcaagcga tcgaccaaga cccgtacttc cgccttacgc gcgacgtggc gccgcgcatg ggctatttga aaccagccgt cattcactcc aagttcttcc cgggtcttag tgggtcgaaa ggcaagatga gctcatccac cggcgctgcc gtctttctga cggacacccc aaagatgatc aaagacaaga tcaacaagca cgccttcagc ggcggtggcg cagataagaa ggagcagctg cttcttggtg gtaacaccaa cgtcgacgta cctgtgcagt ggctccgctt tttcctcgag gatgacgagg agctgaagcg cctccagaag gactacatgc ttggccgcat catgaccggt gacgtgaaga aagtcctcat ccgacaaatc accagcatcg tgacggcaca ccaggagtcg cggaaaaagg taacggaggc cgatgtggag ctcttcactg ccgtccgcgt gatgggacct gcgaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgt ccccctttcc cccgctgcct gtctatctct ctggcctgct cacccttcat gttcctactc gctgccttca cttccttgtg cttacaccaa tgaaaacccg ggttggactg catcgctgct gcgcatctta cctgcgcttg ctcagccaag atcgactgca gctccacaac ccaatcgaat tcattggcgc actccatttt ttcacctcgc tgtgctctcc gttgggtgtt gccgacttct taagaatgga ggaccccgcc gcgcaggagg ttgtcgtgac cccgtggacg gtcgagggtg acgtgaacta tgacaagctc atcaaaaatt tcgggtgcca ggcgatcgac gacaagctgc tggagcggat ggagcggctg accgggaaga aaccccatca ctttctgcgc cgcggcatct tcttctctca tcgtgatttg aaccttatcc tcgatgcgta cgagaagggg cagcctttct acctgtacac tggccgtggt cccagcagcg agtcgatgca cgttggccac ctgatacctt ttatgttcac caagtggctg caagacacgt tccatgtgcc gctggtcatt cagcttaccg atgacgaaaa gttcttcttc aaggacttga cgatggagga gatcgataaa atgaccacgg aaaacttgaa ggacatcatt gcctttggtt ttgatccgaa gctgacgttc attttcaggg actttgagta cgtcggacag atgtatcgca ttgtcgcacg catcgagaag gcgtacacgg ctagccaggt gcggggctgc ttcggcttca agatggagga caactgcggc cgctggatgt tccctgcgat tcaggccgca ccctctttct cggcggcgtt tccacacatt tttccgctgc agaagggcaa cgtcttctgc atgataccgc aagcgatcga ccaagacccg tacttccgcc ttacgcgcga cgtggcgccg cgcatgggct atttgaaacc agccgtcatt cactccaagt tcttcccggg tcttagtggg tcgaaaggca agatgagctc atccaccggc gctgccgtct ttctgacgga caccccaaag atgatcaaag acaagatcaa caagcacgcc ttcagcggcg gtggcgcaga taagaaggag cagctgcttc ttggtggtaa caccaacgtc gacgtacctg tgcagtggct ccgctttttc ctcgaggatg acgaggagct gaagcgcctc cagaaggact acatgcttgg ccgcatcatg accggtgacg tgaagaaagt cctcatccga caaatcacca gcatcgtgac ggcacaccag gagtcgcgga aaaaggtaac ggaggccgat gtggagctct tcactgccgt ccgcgtgatg ggacctgcga aagaggaggc ggagtcaagg aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat