LemaA.00437.b

cell division protein kinase 2

CENTER ID: LemaA.00437.b
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00437.b.B1.GE40686 full length 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LmjF36.0550
RefSeq: XP_001686627.1
UniProt: O96526

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSFGRVTAR SGDAGTRDSL DRYNRLDVLG EGTYGVVYRA VDKITGQYVA LKKVRLDRTE EGIPQTALRE VSILQEFDHP NIVNLLDVIC SDGKLYLVFE YVEADLKKAI EKQEGGYSGM DLKRLIYQLL DGLYFCHRHR IIHRDLKPAN ILLTSGNVLK LADFGLARAF QVPMHTYTHE VVTLWYRAPE ILLGEKHYTP AVDMWSVGCI FAELTRRKVL FRGDSEIGQL FEIFQVLGTP TDTEGSWPGV SRLPDYRDVF PKWTAKRLGQ VLPELHPDAI DLLSKMLKYD PRERISAKEA LQHPWFSDLR W
NT Sequence
atgtcttcgt ttggccgtgt taccgcccgt agcggtgacg ctgggacgcg tgacagtctt gaccggtaca atcgcttgga tgttttggga gagggaacgt atggtgttgt gtatcgcgcg gtcgacaaaa tcactgggca gtacgttgct ctcaagaaag tgcgactcga tcgcactgag gagggcattc cgcagactgc gctgcgcgag gtgtcgattc tacaagagtt cgaccacccc aacattgtga acttgcttga tgtcatttgc tcggacggga agctctacct tgtcttcgag tatgtggagg cggacctgaa aaaggcgatt gaaaagcaag agggtggata ctctggaatg gatctgaagc ggcttattta tcagctttta gacggccttt acttttgcca ccgccatcgc atcatccacc gtgatctgaa gccagccaac atcctcctga catcagggaa cgtccttaaa ttggctgatt tcggtctcgc ccgtgcgttc caagtgccca tgcacaccta cacgcacgag gtggttacac tgtggtaccg tgcccctgag atcctcctcg gtgagaagca ctacactcct gccgtggata tgtggagtgt cggctgtatt ttcgctgagc taacgcgccg aaaggttctt ttccgcggcg atagcgaaat cgggcagttg ttcgagattt ttcaagtgct ggggacgccg acggacaccg aggggtcctg gcctggtgtg tcgcggctgc ccgactaccg cgacgtattt cccaagtgga ctgcaaagcg cctggggcag gtactaccag aacttcatcc ggacgctatt gatctgctct ccaagatgct caagtacgat ccgcgggagc gcatatcagc caaggaggcc cttcagcacc cgtggttcag tgaccttcgc tggtag
Details for LemaA.00437.b.B1.GE40686
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHXX SFGRVTARSG DAGTRDSLDR YNRLDVLGEG TYGVVYRAVD KITGQYVALK KVRLDRTEEG IPQTALREVS ILQEFDHPNI VNLLDVICSD GKLYLVFEYV EADLKKAIEK QEGGYSGMDL KRLIYQLLDG LYFCHRHRII HRDLKPANIL LTSGNVLKLA DFGLARAFQV PMHTYTHEVV TLWYRAPEIL LGEKHYTPAV DMWSVGCIFA ELTRRKVLFR GDSEIGQLFE IFQVLGTPTD TEGSWPGVSR LPDYRDVFPK WTAKRLGQVL PELHPDAIDL LSKMLKYDPR ERISAKEALQ HPXXSDLRW
Validated NT Sequence
atggctcacc accaccacca ccatatnnnt tcgtttggcc gtgttaccgc ccgtagcggt gacgctggga cgcgtgacag tcttgaccgg tacaatcgct tggatgtttt gggagaggga acgtatggtg ttgtgtatcg cgcggtcgac aaaatcactg ggcagtacgt tgctctcaag aaagtgcgac tcgatcgcac tgaggagggc attccgcaga ctgcgctgcg cgaggtgtcg attctacaag agttcgacca ccccaacatt gtgaacttgc ttgatgtcat ttgctcggac gggaagctct accttgtctt cgagtatgtg gaggcggacc tgaaaaaggc gattgaaaag caagagggtg gatactctgg aatggatctg aagcggctta tttatcagct tttagacggc ctttactttt gccaccgcca tcgcatcatc caccgtgatc tgaagccagc caacatcctc ctgacatcag ggaacgtcct taaattggct gatttcggtc tcgcccgtgc gttccaagtg cccatgcaca cctacacgca cgaggtggtt acactgtggt accgtgcccc tgagatcctc ctcggtgaga agcactacac tcctgccgtg gatatgtgga gtgtcggctg tattttcgct gagctaacgc gccgaaaggt tcttttccgc ggcgatagcg aaatcgggca gttgttcgag atttttcaag tgctggggac gccgacggac accgaggggt cctggcctgg tgtgtcgcgg ctgcccgact accgcgacgt atttcccaag tggactgcaa agcgcctggg gcaggtacta ccagaacttc atccggacgc tattgatctg ctctccaaga tgctcaagta cgatccgcgg gagcgcatat cagccaagga ggcccttcag cacccnnnnn nnagtgacct tcgctggtaa gtgagtaa
Expected Protein Sequence
MAHHHHHHMS SFGRVTARSG DAGTRDSLDR YNRLDVLGEG TYGVVYRAVD KITGQYVALK KVRLDRTEEG IPQTALREVS ILQEFDHPNI VNLLDVICSD GKLYLVFEYV EADLKKAIEK QEGGYSGMDL KRLIYQLLDG LYFCHRHRII HRDLKPANIL LTSGNVLKLA DFGLARAFQV PMHTYTHEVV TLWYRAPEIL LGEKHYTPAV DMWSVGCIFA ELTRRKVLFR GDSEIGQLFE IFQVLGTPTD TEGSWPGVSR LPDYRDVFPK WTAKRLGQVL PELHPDAIDL LSKMLKYDPR ERISAKEALQ HPWFSDLRW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt cgtttggccg tgttaccgcc cgtagcggtg acgctgggac gcgtgacagt cttgaccggt acaatcgctt ggatgttttg ggagagggaa cgtatggtgt tgtgtatcgc gcggtcgaca aaatcactgg gcagtacgtt gctctcaaga aagtgcgact cgatcgcact gaggagggca ttccgcagac tgcgctgcgc gaggtgtcga ttctacaaga gttcgaccac cccaacattg tgaacttgct tgatgtcatt tgctcggacg ggaagctcta ccttgtcttc gagtatgtgg aggcggacct gaaaaaggcg attgaaaagc aagagggtgg atactctgga atggatctga agcggcttat ttatcagctt ttagacggcc tttacttttg ccaccgccat cgcatcatcc accgtgatct gaagccagcc aacatcctcc tgacatcagg gaacgtcctt aaattggctg atttcggtct cgcccgtgcg ttccaagtgc ccatgcacac ctacacgcac gaggtggtta cactgtggta ccgtgcccct gagatcctcc tcggtgagaa gcactacact cctgccgtgg atatgtggag tgtcggctgt attttcgctg agctaacgcg ccgaaaggtt cttttccgcg gcgatagcga aatcgggcag ttgttcgaga tttttcaagt gctggggacg ccgacggaca ccgaggggtc ctggcctggt gtgtcgcggc tgcccgacta ccgcgacgta tttcccaagt ggactgcaaa gcgcctgggg caggtactac cagaacttca tccggacgct attgatctgc tctccaagat gctcaagtac gatccgcggg agcgcatatc agccaaggag gcccttcagc acccgtggtt cagtgacctt cgctggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t