LemaA.00436.b

serine/threonine kinase, putative

CENTER ID: LemaA.00436.b
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00436.b.B1.GE34424 full length 1 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00436.b.B1.PS01495 full length 1 271

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LmjF18.0640
RefSeq: XP_001682471.1
UniProt: Q4QDX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPSVMVGQY TLGKQIGSGN FSKVRLGTDP QGRTWAIKIV DKRRLKKENM EDQMLREVAI MRSLRQQNVV KLQEVLQSSN HYYLVLELVT GGELFDKIVA AKRFDEPTAR RYFHQLIAGM YYCHSKGFAH RDLKPENLLL DANGTLKISD FGLSNLQQDV LLQTICGTPN YVAPEVLMER GYNGLSADIW SCGVVLYVML AGRLPFEDRN MNVLLGKIER GDYQMIRHIS DPAKDLVARM LTVDPRKRIS MEDVINHPWF QIDWNPRLLS T
NT Sequence
atggacccat ctgtcatggt gggccagtac acgctgggca agcagatcgg ctccggcaac ttctcgaagg tgcgactggg caccgatccg caaggccgca catgggccat caagatcgtg gacaagcgcc gcctaaagaa ggagaacatg gaagaccaga tgctccgcga agtcgccatc atgcgcagtc tgcgccagca gaacgtggtg aaactccagg aggtgctcca gtcctctaac cattactacc tagtcctcga gctggtcaca ggcggcgagc tgtttgacaa aattgtagcc gccaagcgct tcgacgagcc cacagcgcgt cggtacttcc accagctcat cgcgggcatg tactactgtc actcgaaggg cttcgcccac cgcgacctga agccggagaa cctgttgctc gacgcgaacg gcacactgaa gatttccgac tttggactca gcaacctcca gcaggatgtg ctgctgcaga ccatctgcgg cacgccaaac tacgttgcgc cggaggtgct catggagcgc ggctacaacg gactctcggc ggacatctgg agctgcggtg tggtgctgta cgtgatgctg gcaggacgtc tgcccttcga ggatcggaac atgaacgttc ttcttggcaa gatcgagcgt ggcgactatc aaatgatccg tcacatcagc gaccctgcca aggacctcgt cgcgcgcatg ctgaccgtcg acccgcgcaa gcgcatctca atggaggatg tcatcaacca cccttggttc cagattgact ggaatccgag gctactctcc acgtaa
Details for LemaA.00436.b.B1.GE34424
HARVESTED ON: 2/6/2012
SEQUENCED ON: 2/21/2012
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD PSVMVGQYTL GKQIGSGNFS KVRLGTDPQG RTWAIKIVDK RRLKKENMED QMLREVAIMR SLRQQNVVKL QEVLQSSNHY YLVLELVTGG ELFDKIVAAK RFDEPTARRY FHQLIAGMYY CHSKGFAHRD LKPENLLLDA NGTLKISDFG LSNLQQDVLL QTICGTPNYV APEVLMERGY NGLSADIWSC GVVLYVMLAG RLPFEDRNMN VLLGKIERGD YQMIRHISDP AKDLVARMLT VDPRKRISME DVINHPWFQI DWNPRLLST
Validated NT Sequence
tacgtggaga gtagcctcgg attccagtca atctggaacc aagggtggtt gatgacatcc tccattgaga tgcgcttgcg cgggtcgacg gtcagcatgc gcgcgacgag gtccttggca gggtcgctga tgtgacggat catttgatag tcgccacgct cgatcttgcc aagaagaacg ttcatgttcc gatcctcgaa gggcagacgt cctgccagca tcacgtacag caccacaccg cagctccaga tgtccgccga gagtccgttg tagccgcgct ccatgagcac ctccggcgca acgtagtttg gcgtgccgca gatggtctgc agcagcacat cctgctggag gttgctgagt ccaaagtcgg aaatcttcag tgtgccgttc gcgtcgagca acaggttctc cggcttcagg tcgcggtggg cgaagccctt cgagtgacag tagtacatgc ccgcgatgag ctggtggaag taccgacgcg ctgtgggctc gtcgaagcgc ttggcggcta caattttgtc aaacagctcg ccgcctgtga ccagctcgag gactaggtag taatggttag aggactggag cacctcctgg agtttcacca cgttctgctg gcgcagactg cgcatgatgg cgacttcgcg gagcatctgg tcttccatgt tctccttctt taggcggcgc ttgtccacga tcttgatggc ccatgtgcgg ccttgcggat cggtgcccag tcgcaccttc gagaagttgc cggagccgat ctgcttgccc agcgtgtact ggcccaccat gacagatggg tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMD PSVMVGQYTL GKQIGSGNFS KVRLGTDPQG RTWAIKIVDK RRLKKENMED QMLREVAIMR SLRQQNVVKL QEVLQSSNHY YLVLELVTGG ELFDKIVAAK RFDEPTARRY FHQLIAGMYY CHSKGFAHRD LKPENLLLDA NGTLKISDFG LSNLQQDVLL QTICGTPNYV APEVLMERGY NGLSADIWSC GVVLYVMLAG RLPFEDRNMN VLLGKIERGD YQMIRHISDP AKDLVARMLT VDPRKRISME DVINHPWFQI DWNPRLLST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacc catctgtcat ggtgggccag tacacgctgg gcaagcagat cggctccggc aacttctcga aggtgcgact gggcaccgat ccgcaaggcc gcacatgggc catcaagatc gtggacaagc gccgcctaaa gaaggagaac atggaagacc agatgctccg cgaagtcgcc atcatgcgca gtctgcgcca gcagaacgtg gtgaaactcc aggaggtgct ccagtcctct aaccattact acctagtcct cgagctggtc acaggcggcg agctgtttga caaaattgta gccgccaagc gcttcgacga gcccacagcg cgtcggtact tccaccagct catcgcgggc atgtactact gtcactcgaa gggcttcgcc caccgcgacc tgaagccgga gaacctgttg ctcgacgcga acggcacact gaagatttcc gactttggac tcagcaacct ccagcaggat gtgctgctgc agaccatctg cggcacgcca aactacgttg cgccggaggt gctcatggag cgcggctaca acggactctc ggcggacatc tggagctgcg gtgtggtgct gtacgtgatg ctggcaggac gtctgccctt cgaggatcgg aacatgaacg ttcttcttgg caagatcgag cgtggcgact atcaaatgat ccgtcacatc agcgaccctg ccaaggacct cgtcgcgcgc atgctgaccg tcgacccgcg caagcgcatc tcaatggagg atgtcatcaa ccacccttgg ttccagattg actggaatcc gaggctactc tccacgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for LemaA.00436.b.B1.PS01495
PURIFICATION DATe: 5/17/2012
CONCENTRATION: 18.87mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 150µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD PSVMVGQYTL GKQIGSGNFS KVRLGTDPQG RTWAIKIVDK RRLKKENMED QMLREVAIMR SLRQQNVVKL QEVLQSSNHY YLVLELVTGG ELFDKIVAAK RFDEPTARRY FHQLIAGMYY CHSKGFAHRD LKPENLLLDA NGTLKISDFG LSNLQQDVLL QTICGTPNYV APEVLMERGY NGLSADIWSC GVVLYVMLAG RLPFEDRNMN VLLGKIERGD YQMIRHISDP AKDLVARMLT VDPRKRISME DVINHPWFQI DWNPRLLST
Validated NT Sequence
tacgtggaga gtagcctcgg attccagtca atctggaacc aagggtggtt gatgacatcc tccattgaga tgcgcttgcg cgggtcgacg gtcagcatgc gcgcgacgag gtccttggca gggtcgctga tgtgacggat catttgatag tcgccacgct cgatcttgcc aagaagaacg ttcatgttcc gatcctcgaa gggcagacgt cctgccagca tcacgtacag caccacaccg cagctccaga tgtccgccga gagtccgttg tagccgcgct ccatgagcac ctccggcgca acgtagtttg gcgtgccgca gatggtctgc agcagcacat cctgctggag gttgctgagt ccaaagtcgg aaatcttcag tgtgccgttc gcgtcgagca acaggttctc cggcttcagg tcgcggtggg cgaagccctt cgagtgacag tagtacatgc ccgcgatgag ctggtggaag taccgacgcg ctgtgggctc gtcgaagcgc ttggcggcta caattttgtc aaacagctcg ccgcctgtga ccagctcgag gactaggtag taatggttag aggactggag cacctcctgg agtttcacca cgttctgctg gcgcagactg cgcatgatgg cgacttcgcg gagcatctgg tcttccatgt tctccttctt taggcggcgc ttgtccacga tcttgatggc ccatgtgcgg ccttgcggat cggtgcccag tcgcaccttc gagaagttgc cggagccgat ctgcttgccc agcgtgtact ggcccaccat gacagatggg tccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMD PSVMVGQYTL GKQIGSGNFS KVRLGTDPQG RTWAIKIVDK RRLKKENMED QMLREVAIMR SLRQQNVVKL QEVLQSSNHY YLVLELVTGG ELFDKIVAAK RFDEPTARRY FHQLIAGMYY CHSKGFAHRD LKPENLLLDA NGTLKISDFG LSNLQQDVLL QTICGTPNYV APEVLMERGY NGLSADIWSC GVVLYVMLAG RLPFEDRNMN VLLGKIERGD YQMIRHISDP AKDLVARMLT VDPRKRISME DVINHPWFQI DWNPRLLST
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACC CATCTGTCAT GGTGGGCCAG TACACGCTGG GCAAGCAGAT CGGCTCCGGC AACTTCTCGA AGGTGCGACT GGGCACCGAT CCGCAAGGCC GCACATGGGC CATCAAGATC GTGGACAAGC GCCGCCTAAA GAAGGAGAAC ATGGAAGACC AGATGCTCCG CGAAGTCGCC ATCATGCGCA GTCTGCGCCA GCAGAACGTG GTGAAACTCC AGGAGGTGCT CCAGTCCTCT AACCATTACT ACCTAGTCCT CGAGCTGGTC ACAGGCGGCG AGCTGTTTGA CAAAATTGTA GCCGCCAAGC GCTTCGACGA GCCCACAGCG CGTCGGTACT TCCACCAGCT CATCGCGGGC ATGTACTACT GTCACTCGAA GGGCTTCGCC CACCGCGACC TGAAGCCGGA GAACCTGTTG CTCGACGCGA ACGGCACACT GAAGATTTCC GACTTTGGAC TCAGCAACCT CCAGCAGGAT GTGCTGCTGC AGACCATCTG CGGCACGCCA AACTACGTTG CGCCGGAGGT GCTCATGGAG CGCGGCTACA ACGGACTCTC GGCGGACATC TGGAGCTGCG GTGTGGTGCT GTACGTGATG CTGGCAGGAC GTCTGCCCTT CGAGGATCGG AACATGAACG TTCTTCTTGG CAAGATCGAG CGTGGCGACT ATCAAATGAT CCGTCACATC AGCGACCCTG CCAAGGACCT CGTCGCGCGC ATGCTGACCG TCGACCCGCG CAAGCGCATC TCAATGGAGG ATGTCATCAA CCACCCTTGG TTCCAGATTG ACTGGAATCC GAGGCTACTC TCCACGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T