LemaA.00429.a

ATP-dependent 6-phosphofructokinase (ATP-PFK) (Phosphofructokinase) (EC 2.7.1.11) (Phosphohexokinase) (Phosphofructokinase)

CENTER ID: LemaA.00429.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00429.a.B1.GE42077 full length 1 486
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.29_2510
UniProt: E9AEB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
METRHHLNTK MVPSYQAPLS KVTAADLTVE RLPGCKYMNP SKKHILRKEY RDKVEHIMYD PRPQEDLDAE YPVSCNKLVC ELAAARKHLH FNPSETSIGI VTCGGICPGL NDVIRSITLT GIISYRVKRV VGFRYGYWGL SKEGSKTAIE LSRTDVRQIH RFGGTILGSS RGPQSPKEMV DTLVRMKINI LFTVGGDGTQ RGALTIYEEA KRRGENIAVF GVPKTIDNDL AFSHRTFGFQ TAVEQAVNAV RAAYAEAVSL NYGVGIVKLM GRESGFIAAQ TTVASAQANI CLIPENPLPK ETVMRLIERR LQQSRNCVIV VAEGFGQDWE TGTGGHDASG NKKLVDIGFI LKKEVEGWLR ANKEKFPQGT VKYIDPSYMI RACPPSSNDA LFCTNLATLA VHEAMAGATG CIISMRYNNY ILVPIKAATS VRRVVSLRGA LWRQVREITV GLSDDVQQWN EQDLRRHLES LNVERERIIA RLASKV
NT Sequence
atggagactc gccaccatct gaacactaag atggtgccgt cgtaccaggc accgctgtcc aaggtgaccg ccgccgacct gacggtggag cgcctgcccg gctgcaagta catgaacccc tcaaagaagc acattctccg taaggagtac cgcgacaagg tggagcacat catgtacgac cctcgcccgc aggaggacct ggacgccgag tacccggtgt cgtgcaacaa gctggtgtgc gagttggctg ccgcccgcaa gcacctccac ttcaaccctt ccgagacctc catcggtatc gtgacctgcg gcggcatctg ccctggtctg aacgacgtca tccgctccat cacgctgacg ggcatcatat cctaccgcgt caagcgcgtt gtcggtttcc gttacggcta ctggggcctc tcgaaggagg gcagcaagac tgccatagag ctgtcccgca cggatgtgcg tcagatccac cgcttcggcg gcacgatcct gggtagctct cgcggtccgc agagcccgaa ggagatggtc gacacgcttg tgcgcatgaa gatcaacatt cttttcaccg ttggtggtga tggcacgcag cgcggtgctc taacgatcta cgaagaggcc aagcgccggg gcgagaacat cgccgtcttt ggtgtgccca agacgatcga taacgatctt gctttctccc accgcacctt tggctttcag actgctgtcg agcaggctgt gaacgccgtg cgtgccgcct acgccgaggc tgtgtctctc aactacggtg tcggcatcgt gaagttgatg ggccgtgaga gcggtttcat tgctgcccag acgactgtgg ccagcgcgca ggcgaacatc tgcctcattc ctgagaaccc actgccaaag gagacggtga tgcgcctgat cgagcgccgc cttcagcagt cgcgcaactg cgtcatcgtg gttgccgagg gcttcggcca ggactgggag accggcacgg gcggccacga cgcctccggt aacaagaagc tcgtcgacat tggcttcatc ctgaagaagg aggtggaggg ctggctgcgc gcgaacaagg agaagttccc gcagggtact gtcaagtata tagacccctc gtacatgatc cgcgcctgcc cgccgtcttc caacgacgcg ctcttctgca ccaacctggc aactcttgct gtgcatgagg ccatggccgg tgccactggc tgcattatct caatgcgcta caacaactat attctcgtgc ccatcaaggc ggccacgtcg gtgcgccggg tcgttagtct ccgtggcgcc ctctggcgcc aggtgcgtga gatcaccgtg ggtctgagcg acgatgtgca gcagtggaac gagcaggacc tgcgccgcca cctggagtcg ctgaatgttg agcgcgagcg cattattgcg cgcctggcgt ctaaggtgta a
Details for LemaA.00429.a.B1.GE42077
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 55kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME TRHHLNTKMV PSYQAPLSKV TAADLTVERL PGCKYMNPSK KHILRKEYRD KVEHIMYDPR PQEDLDAEYP VSCNKLVCEL AAARKHLHFN PSETSIGIVT CGGICPGLND VIRSITLTGI ISYRVKRVVG FRYGYWGLSK EGSKTAIELS RTDVRQIHRF GGTILGSSRG PQSPKEMVDT LVRMKINILF TVGGDGTQRG ALTIYXXAKR RGENIAVFGV PKTIDNDLAF SHRTFGFQTA VEQAVNAVRA AYAEAVSLNY GVGIVKLMGR ESGFIAAQTT VASAQANICL IPENPLPKET VMRLIERRLQ QSRNCVIVVA EGFGQDWETG TGGHDASGNK KLVDIGFILK KEVEGWLRAN KEKFPQGTVK YIDPSYMIRA CPPSSNDALF CTNLATLAVH EAMAGATGCI ISMRYNNYIL VPIKAATSVR RVVSLRGALW RQVREITVGL SDDVQQWNEQ DLRRHLESLN VERERII
Validated NT Sequence
caataatgcg ctcgcgctca acattcagcg actccaggtg gcggcgcagg tcctgctcgt tccactgctg cacatcgtcg ctcagaccca cggtgatctc acgcacctgg cgccagaggg cgccacggag actaacgacc cggcgcaccg acgtggccgc cttgatgggc acgagaatat agttgttgta gcgcattgag ataatgcagc cagtggcacc ggccatggcc tcatgcacag caagagttgc caggttggtg cagaagagcg cgtcgttgga agacggcggg caggcgcgga tcatgtacga ggggtctata tacttgacag taccctgcgg gaacttctcc ttgttcgcgc gcagccagcc ctccacctcc ttcttcagga tgaagccaat gtcgacgagc ttcttgttac cggaggcgtc gtggccgccc gtgccggtct cccagtcctg gccgaagccc tcggcaacca cgatgacgca gttgcgcgac tgctgaaggc ggcgctcgat caggcgcatc accgtctcct ttggcagtgg gttctcagga atgaggcaga tgttcgcctg cgcgctggcc acagtcgtct gggcagcaat gaaaccgctc tcacggccca tcaacttcac gatgccgaca ccgtagttga gagacacagc ctcggcgtag gcggcacgca cggcgttcac agcctgctcg acagcagtct gaaagccaaa ggtgcggtgg gagaaagcaa gatcgttatc gatcgtcttg ggcacaccaa agacggcgat gttctcgccc cggcgcttgg cnnnnnngta gatcgttaga gcaccgcgct gcgtgccatc accaccaacg gtgaaaagaa tgttgatctt catgcgcaca agcgtgtcga ccatctcctt cgggctctgc ggaccgcgag agctacccag gatcgtgccg ccgaagcggt ggatctgacg cacatccgtg cgggacagct ctatggcagt cttgctgccc tccttcgaga ggccccagta gccgtaacgg aaaccgacaa cgcgcttgac gcggtaggat atgatgcccg tcagcgtgat ggagcggatg acgtcgttca gaccagggca gatgccgccg caggtcacga taccgatgga ggtctcggaa gggttgaagt ggaggtgctt gcgggcggca gccaactcgc acaccagctt gttgcacgac accgggtact cggcgtccag gtcctcctgc gggcgagggt cgtacatgat gtgctccacc ttgtcgcggt actccttacg gagaatgtgc ttctttgagg ggttcatgta cttgcagccg ggcaggcgct ccaccgtcag gtcggcggcg gtcaccttgg acagcggtgc ctggtacgac ggcaccatct tagtgttcag atggtggcga gtctccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHME TRHHLNTKMV PSYQAPLSKV TAADLTVERL PGCKYMNPSK KHILRKEYRD KVEHIMYDPR PQEDLDAEYP VSCNKLVCEL AAARKHLHFN PSETSIGIVT CGGICPGLND VIRSITLTGI ISYRVKRVVG FRYGYWGLSK EGSKTAIELS RTDVRQIHRF GGTILGSSRG PQSPKEMVDT LVRMKINILF TVGGDGTQRG ALTIYEEAKR RGENIAVFGV PKTIDNDLAF SHRTFGFQTA VEQAVNAVRA AYAEAVSLNY GVGIVKLMGR ESGFIAAQTT VASAQANICL IPENPLPKET VMRLIERRLQ QSRNCVIVVA EGFGQDWETG TGGHDASGNK KLVDIGFILK KEVEGWLRAN KEKFPQGTVK YIDPSYMIRA CPPSSNDALF CTNLATLAVH EAMAGATGCI ISMRYNNYIL VPIKAATSVR RVVSLRGALW RQVREITVGL SDDVQQWNEQ DLRRHLESLN VERERIIARL ASKV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaga ctcgccacca tctgaacact aagatggtgc cgtcgtacca ggcaccgctg tccaaggtga ccgccgccga cctgacggtg gagcgcctgc ccggctgcaa gtacatgaac ccctcaaaga agcacattct ccgtaaggag taccgcgaca aggtggagca catcatgtac gaccctcgcc cgcaggagga cctggacgcc gagtacccgg tgtcgtgcaa caagctggtg tgcgagttgg ctgccgcccg caagcacctc cacttcaacc cttccgagac ctccatcggt atcgtgacct gcggcggcat ctgccctggt ctgaacgacg tcatccgctc catcacgctg acgggcatca tatcctaccg cgtcaagcgc gttgtcggtt tccgttacgg ctactggggc ctctcgaagg agggcagcaa gactgccata gagctgtccc gcacggatgt gcgtcagatc caccgcttcg gcggcacgat cctgggtagc tctcgcggtc cgcagagccc gaaggagatg gtcgacacgc ttgtgcgcat gaagatcaac attcttttca ccgttggtgg tgatggcacg cagcgcggtg ctctaacgat ctacgaagag gccaagcgcc ggggcgagaa catcgccgtc tttggtgtgc ccaagacgat cgataacgat cttgctttct cccaccgcac ctttggcttt cagactgctg tcgagcaggc tgtgaacgcc gtgcgtgccg cctacgccga ggctgtgtct ctcaactacg gtgtcggcat cgtgaagttg atgggccgtg agagcggttt cattgctgcc cagacgactg tggccagcgc gcaggcgaac atctgcctca ttcctgagaa cccactgcca aaggagacgg tgatgcgcct gatcgagcgc cgccttcagc agtcgcgcaa ctgcgtcatc gtggttgccg agggcttcgg ccaggactgg gagaccggca cgggcggcca cgacgcctcc ggtaacaaga agctcgtcga cattggcttc atcctgaaga aggaggtgga gggctggctg cgcgcgaaca aggagaagtt cccgcagggt actgtcaagt atatagaccc ctcgtacatg atccgcgcct gcccgccgtc ttccaacgac gcgctcttct gcaccaacct ggcaactctt gctgtgcatg aggccatggc cggtgccact ggctgcatta tctcaatgcg ctacaacaac tatattctcg tgcccatcaa ggcggccacg tcggtgcgcc gggtcgttag tctccgtggc gccctctggc gccaggtgcg tgagatcacc gtgggtctga gcgacgatgt gcagcagtgg aacgagcagg acctgcgccg ccacctggag tcgctgaatg ttgagcgcga gcgcattatt gcgcgcctgg cgtctaaggt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat