LemaA.00424.a

serine/threonine-protein kinase, putative

CENTER ID: LemaA.00424.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00424.a.B2.GE40014 PK-like domain 45 343
LemaA.00424.a.B3.GE40685 putative kinase domain 41 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LmjF25.2340
RefSeq: XP_001683996.1
UniProt: Q4Q9K2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFMKLFKKKE KKDKEEDKKS ESTPTLENKR VETPEAPEKL TKDDFETLDV LGKGSFAYVV LCRRLSTNKY YAMKVINKQG LLDHKRVQDV FTERNVLTRL NHPYLLKLYW TFQSEHKLFF VMDYMPGGDL DKYMNSVPKK QLDPATAQLY GAEILLAIMC LHEHSVIYRD LKPENILLDG EGHCALADFG LSKDFHKDGQ DVSDKELRAN SFVGSPFYVA PDVLRQREYT NAVDFWSFGI LLYRMLCGRT PFNGRSMTEV FDNILYSDLS FPSGVAVSPE AKDLISRLLM KDPNRRIKGP EVKQHPYWTG ISFEEVAQRK IAPSRWTPLP SVEEMLAARK NVSAGPSTSL KNPHHVVNTP AQSSQLNDGQ QRLFGGFSCT ADSHLNNS
NT Sequence
atgttcatga aacttttcaa gaagaaggaa aagaaggaca aagaggagga caagaagtcc gagtcgacgc ctaccttgga gaacaagcgt gtggaaaccc ctgaggcgcc ggagaagctg accaaagacg acttcgagac cctcgatgtc ctcggcaagg gctcctttgc ctacgtggtg ctgtgccgtc gcctctccac gaacaagtac tacgccatga aggtgatcaa caagcagggc ctgctggacc acaagcgagt gcaggacgtc tttacggagc gcaacgtgct gactcgcctg aaccacccct acttgctgaa actgtactgg accttccagt cggagcacaa gctgttcttc gtcatggact acatgcctgg cggtgacctc gacaagtaca tgaactccgt cccgaagaag cagctcgacc cggcaacagc gcagctgtac ggtgccgaga tcctcttggc cattatgtgc ctgcacgagc acagcgtcat atatcgagat ctaaagccag agaacattct gctggatggt gagggccact gcgctcttgc agacttcggc ctgtccaagg atttccacaa ggacggccag gacgtctccg acaaggaact gcgcgccaac tcgttcgtcg ggtcgccgtt ctacgtggct cctgacgtgc tgcgccaacg tgagtacacc aacgcagtgg acttctggtc cttcggcatt ctgctgtacc gcatgctgtg cggccgcact cccttcaatg gccgcagcat gacggaggtg ttcgacaaca tcttgtactc tgacctctct ttccccagtg gcgtggcggt gtcgccggaa gccaaggatc tgatcagccg cttgctcatg aaggacccca accgtcgcat caagggccct gaggtgaagc agcaccccta ctggaccggg atcagctttg aggaggtggc gcagcgcaag attgccccat cgcggtggac cccactgccg tcggtggagg agatgctggc tgcgcgcaaa aacgtcagcg ctggcccctc gacctctttg aagaacccgc accacgtcgt gaatacgccg gcacagagct cgcagctcaa cgatggccag cagaggttgt ttggcggctt ctcctgcacg gcggacagcc acctgaacaa cagctga
Details for LemaA.00424.a.B2.GE40014
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF ETLDVLGKGS FAYVVLCRRL STNKYYAMKV INKQGLLDHK RVQDVFTERN VLTRLNHPYL LKLYWTFQSE HKLFFVMDYM PGGDLDKYMN SVPKKQLDPA TAQLYGAEIL LAIMCLHEHS VIYRDLKPEN ILLDGEGHCA LADFGLSKDF HKDGQDVSDK ELRANSFVGS PFYVAPDVLR QREYTNAVDF WSFGILLYRM LCGRTPFNGR SMTEVFDNIL YSDLSFPSGV AVSPEAKDLI SRLLMKDPNR RIKGPEVKQH PYWTGISFEE VAQRKIAPSR WTPLPSVEEX XXARKNVS
Validated NT Sequence
ttactcactt agctgacgtt tttgcgcgca nnnnnnatct cctccaccga cggcagtggg gtccaccgcg atggggcaat cttgcgctgc gccacctcct caaagctgat cccggtccag taggggtgct gcttcacctc agggcccttg atgcgacggt tggggtcctt catgagcaag cggctgatca gatccttggc ttccggcgac accgccacgc cactggggaa agagaggtca gagtacaaga tgttgtcgaa cacctccgtc atgctgcggc cattgaaggg agtgcggccg cacagcatgc ggtacagcag aatgccgaag gaccagaagt ccactgcgtt ggtgtactca cgttggcgca gcacgtcagg agccacgtag aacggcgacc cgacgaacga gttggcgcgc agttccttgt cggagacgtc ctggccgtcc ttgtggaaat ccttggacag gccgaagtct gcaagagcgc agtggccctc accatccagc agaatgttct ctggctttag atctcgatat atgacgctgt gctcgtgcag gcacataatg gccaagagga tctcggcacc gtacagctgc gctgttgccg ggtcgagctg cttcttcggg acggagttca tgtacttgtc gaggtcaccg ccaggcatgt agtccatgac gaagaacagc ttgtgctccg actggaaggt ccagtacagt ttcagcaagt aggggtggtt caggcgagtc agcacgttgc gctccgtaaa gacgtcctgc actcgcttgt ggtccagcag gccctgcttg ttgatcacct tcatggcgta gtacttgttc gtggagaggc gacggcacag caccacgtag gcaaaggagc ccttgccgag gacatcgagg gtctcgaaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHFE TLDVLGKGSF AYVVLCRRLS TNKYYAMKVI NKQGLLDHKR VQDVFTERNV LTRLNHPYLL KLYWTFQSEH KLFFVMDYMP GGDLDKYMNS VPKKQLDPAT AQLYGAEILL AIMCLHEHSV IYRDLKPENI LLDGEGHCAL ADFGLSKDFH KDGQDVSDKE LRANSFVGSP FYVAPDVLRQ REYTNAVDFW SFGILLYRML CGRTPFNGRS MTEVFDNILY SDLSFPSGVA VSPEAKDLIS RLLMKDPNRR IKGPEVKQHP YWTGISFEEV AQRKIAPSRW TPLPSVEEML AARKNVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttcgaga ccctcgatgt cctcggcaag ggctcctttg cctacgtggt gctgtgccgt cgcctctcca cgaacaagta ctacgccatg aaggtgatca acaagcaggg cctgctggac cacaagcgag tgcaggacgt ctttacggag cgcaacgtgc tgactcgcct gaaccacccc tacttgctga aactgtactg gaccttccag tcggagcaca agctgttctt cgtcatggac tacatgcctg gcggtgacct cgacaagtac atgaactccg tcccgaagaa gcagctcgac ccggcaacag cgcagctgta cggtgccgag atcctcttgg ccattatgtg cctgcacgag cacagcgtca tatatcgaga tctaaagcca gagaacattc tgctggatgg tgagggccac tgcgctcttg cagacttcgg cctgtccaag gatttccaca aggacggcca ggacgtctcc gacaaggaac tgcgcgccaa ctcgttcgtc gggtcgccgt tctacgtggc tcctgacgtg ctgcgccaac gtgagtacac caacgcagtg gacttctggt ccttcggcat tctgctgtac cgcatgctgt gcggccgcac tcccttcaat ggccgcagca tgacggaggt gttcgacaac atcttgtact ctgacctctc tttccccagt ggcgtggcgg tgtcgccgga agccaaggat ctgatcagcc gcttgctcat gaaggacccc aaccgtcgca tcaagggccc tgaggtgaag cagcacccct actggaccgg gatcagcttt gaggaggtgg cgcagcgcaa gattgcccca tcgcggtgga ccccactgcc gtcggtggag gagatgctgg ctgcgcgcaa aaacgtcagc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LemaA.00424.a.B3.GE40685
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT KDDFETLDVL GKGSFAYVVL CRRLSTNKYY AMKVINKQGL LDHKRVQDVF TERNVLTRLN HPYLLKLYWT FQSEHKLFFV MDYMPGGDLD KYMNSVPKKQ LDPATAQLYG AEILLAIMCL HEHSVIYRDL KPENILLDGE GHCALADFGL SKDFHKDGQD VSDKELRANS FVGSPFYVAP DVLRQREYTN AVDFWSFGIL LYRMLCGRTP FNGRSMTEVF DNILYSDLSF PSGVAVSPEA KDLISRLLMK DPNRRIKGPE VKQHPYWTGI XXXEVAQ
Validated NT Sequence
atggctcacc accaccacca ccatatgacc aaagacgact tcgagaccct cgatgtcctc ggcaagggct cctttgccta cgtggtgctg tgccgtcgcc tctccacgaa caagtactac gccatgaagg tgatcaacaa gcagggcctg ctggaccaca agcgagtgca ggacgtcttt acggagcgca acgtgctgac tcgcctgaac cacccctact tgctgaaact gtactggacc ttccagtcgg agcacaagct gttcttcgtc atggactaca tgcctggcgg tgacctcgac aagtacatga actccgtccc gaagaagcag ctcgacccgg caacagcgca gctgtacggt gccgagatcc tcttggccat tatgtgcctg cacgagcaca gcgtcatata tcgagatcta aagccagaga acattctgct ggatggtgag ggccactgcg ctcttgcaga cttcggcctg tccaaggatt tccacaagga cggccaggac gtctccgaca aggaactgcg cgccaactcg ttcgtcgggt cgccgttcta cgtggctcct gacgtgctgc gccaacgtga gtacaccaac gcagtggact tctggtcctt cggcattctg ctgtaccgca tgctgtgcgg ccgcactccc ttcaatggcc gcagcatgac ggaggtgttc gacaacatct tgtactctga cctctctttc cccagtggcg tggcggtgtc gccggaagcc aaggatctga tcagccgctt gctcatgaag gaccccaacc gtcgcatcaa gggccctgag gtgaagcagc acccctactg gaccgggatc annnnnnagg aggtggcgca gtaagtgagt aagat
Expected Protein Sequence
MAHHHHHHTK DDFETLDVLG KGSFAYVVLC RRLSTNKYYA MKVINKQGLL DHKRVQDVFT ERNVLTRLNH PYLLKLYWTF QSEHKLFFVM DYMPGGDLDK YMNSVPKKQL DPATAQLYGA EILLAIMCLH EHSVIYRDLK PENILLDGEG HCALADFGLS KDFHKDGQDV SDKELRANSF VGSPFYVAPD VLRQREYTNA VDFWSFGILL YRMLCGRTPF NGRSMTEVFD NILYSDLSFP SGVAVSPEAK DLISRLLMKD PNRRIKGPEV KQHPYWTGIS FEEVAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccaaag acgacttcga gaccctcgat gtcctcggca agggctcctt tgcctacgtg gtgctgtgcc gtcgcctctc cacgaacaag tactacgcca tgaaggtgat caacaagcag ggcctgctgg accacaagcg agtgcaggac gtctttacgg agcgcaacgt gctgactcgc ctgaaccacc cctacttgct gaaactgtac tggaccttcc agtcggagca caagctgttc ttcgtcatgg actacatgcc tggcggtgac ctcgacaagt acatgaactc cgtcccgaag aagcagctcg acccggcaac agcgcagctg tacggtgccg agatcctctt ggccattatg tgcctgcacg agcacagcgt catatatcga gatctaaagc cagagaacat tctgctggat ggtgagggcc actgcgctct tgcagacttc ggcctgtcca aggatttcca caaggacggc caggacgtct ccgacaagga actgcgcgcc aactcgttcg tcgggtcgcc gttctacgtg gctcctgacg tgctgcgcca acgtgagtac accaacgcag tggacttctg gtccttcggc attctgctgt accgcatgct gtgcggccgc actcccttca atggccgcag catgacggag gtgttcgaca acatcttgta ctctgacctc tctttcccca gtggcgtggc ggtgtcgccg gaagccaagg atctgatcag ccgcttgctc atgaaggacc ccaaccgtcg catcaagggc cctgaggtga agcagcaccc ctactggacc gggatcagct ttgaggaggt ggcgcagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at