LemaA.00174.a

protein phosphatase 1 catalytic subunit, putative (PP1C)

CENTER ID: LemaA.00174.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00174.a.B1.GE42327 full length 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.15.0220
UniProt: Q4QFG0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHTKRGRFT VDLSVCALSS LPTDTLSVEK ENDVLYRLLE LLPERYTSKV LEEQRIPEGL IVQILIKARA IIASEPMLVE LDSPVYACGD LHGQYYDLVN IFKRCPPLGG TVFGTDAKKA RKETSSSIDD KTYSFLFLGD YVDRGARSVE VVVTLLALKI ISPRHMTMLR GNHEDEQIML LYGFYDECKR RYDIKLFKMF TDLFRILPVA ALVNGSIFCV HGGLSAELRF VRDIPDTRPC NVPHSGIICD LLWADPETDL PAGVDWAPSS RRISSVFSER ALERFLVEND IDLVCRAHQV VEEGFQFFPD HKKRHLLTIF SATNYCNEFG NRGGILRVDE KGVCSIITLE PPDFVQQRDI MLFRDPLPNP LSHD
NT Sequence
atggcgcaca cgaagcgagg gagattcacc gtggatctgt ccgtgtgtgc gctcagtagc cttcctacag atactctctc tgtggaaaaa gagaatgatg tgctttaccg ccttttagaa cttcttcctg agcgctacac gagcaaagtt ttggaagagc agcgaatccc cgagggtctc atcgtgcaaa ttctcatcaa ggcacgcgct ataatagcca gtgagccgat gcttgtcgaa ttagattccc ctgtttacgc ctgcggtgat ctacacgggc agtactacga cttggttaac attttcaagc ggtgcccacc actgggaggc accgttttcg gaactgatgc gaagaaggca cgtaaagaga ccagctcatc gatcgatgat aaaacataca gttttctctt ccttggcgat tatgtcgacc gcggcgctcg ctccgttgag gtcgttgtga cactgctcgc tctcaagatt atttctccgc ggcacatgac tatgcttcgc ggaaaccacg aggacgaaca aatcatgctc ttgtatgggt tttacgatga atgcaagagg cgctatgaca tcaaactttt caaaatgttc accgatttgt tccgtattct acctgtggcg gccctggtca acggctccat tttctgcgta cacggcggtc tctccgcgga gctccgcttc gtgcgagaca tcccggacac gcgtccgtgc aacgtcccgc actccggcat catttgtgac cttctgtggg cagaccccga aacggatctg ccagctggtg tggactgggc cccgagctcg cgcagaattt catctgtgtt ttccgaacgg gcactggagc ggtttttagt tgagaatgac attgatttgg tgtgccgcgc acatcaggtg gtcgaagaag gattccagtt tttcccggat cacaaaaaac ggcatttgct caccatcttc tcggcaacta actactgcaa cgagttcggg aatagaggcg gcattttacg tgtggatgag aagggtgtat gcagcatcat cacgttggag ccaccagact ttgtccagca gcgggatatc atgctttttc gggatccgct tccaaaccct ctgagccacg actga
Details for LemaA.00174.a.B1.GE42327
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 43kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA HTKRGRFTVD LSVCALSSLP TDTLSVEKEN DVLYRLLXLL PERYTSKVLE EQRIPEGLIV QILIKARAII ASEPMLVELD SPVYACGDLH GQYYDLVNIF KRCPPLGGTV FGTDAKKARK ETSSSIDDKT YSFLFLGDYV DRGARSVEVV VTLLALKIIS PRHMTMLRGN HEDEQIMLLY GFYDECKRRY DIKLFKMFTD LFRILPVAAL VNGSIFCVHG GLSAELRFVR DIPDTRPCNV PHSGIICDLL WADPETDLPA GVDWAPSSRR ISSVFSERAL ERFLVENDID LVCRAHQVVE EGFQFFPDHK KRHLLTIFSA TNYCNEFGNR GGILRVDEKG VCSIITLEPP DFVQQRDIML FRD
Validated NT Sequence
ggatcccgaa aaagcatgat atcccgctgc tggacaaagt ctggtggctc caacgtgatg atgctgcata cacccttctc atccacacgt aaaatgccgc ctctattccc gaactcgttg cagtagttag ttgccgagaa gatggtgagc aaatgccgtt ttttgtgatc cgggaaaaac tggaatcctt cttcgaccac ctgatgtgcg cggcacacca aatcaatgtc attctcaact aaaaaccgct ccagtgcccg ttcggaaaac acagatgaaa ttctgcgcga gctcggggcc cagtccacac cagctggcag atccgtttcg gggtctgccc acagaaggtc acaaatgatg ccggagtgcg ggacgttgca cggacgcgtg tccgggatgt ctcgcacgaa gcggagctcc gcggagagac cgccgtgtac gcagaaaatg gagccgttga ccagggccgc cacaggtaga atacggaaca aatcggtgaa cattttgaaa agtttgatgt catagcgcct cttgcattca tcgtaaaacc catacaagag catgatttgt tcgtcctcgt ggtttccgcg aagcatagtc atgtgccgcg gagaaataat cttgagagcg agcagtgtca caacgacctc aacggagcga gcgccgcggt cgacataatc gccaaggaag agaaaactgt atgttttatc atcgatcgat gagctggtct ctttacgtgc cttcttcgca tcagttccga aaacggtgcc tcccagtggt gggcaccgct tgaaaatgtt aaccaagtcg tagtactgcc cgtgtagatc accgcaggcg taaacagggg aatctaattc gacaagcatc ggctcactgg ctattatagc gcgtgccttg atgagaattt gcacgatgag accctcgggg attcgctgct cttccaaaac tttgctcgtg tagcgctcag gaagaagtnn taaaaggcgg taaagcacat cattctcttt ttccacagag agagtatctg taggaaggct actgagcgca cacacggaca gatccacggt gaatctccct cgcttcgtgt gcgccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMA HTKRGRFTVD LSVCALSSLP TDTLSVEKEN DVLYRLLELL PERYTSKVLE EQRIPEGLIV QILIKARAII ASEPMLVELD SPVYACGDLH GQYYDLVNIF KRCPPLGGTV FGTDAKKARK ETSSSIDDKT YSFLFLGDYV DRGARSVEVV VTLLALKIIS PRHMTMLRGN HEDEQIMLLY GFYDECKRRY DIKLFKMFTD LFRILPVAAL VNGSIFCVHG GLSAELRFVR DIPDTRPCNV PHSGIICDLL WADPETDLPA GVDWAPSSRR ISSVFSERAL ERFLVENDID LVCRAHQVVE EGFQFFPDHK KRHLLTIFSA TNYCNEFGNR GGILRVDEKG VCSIITLEPP DFVQQRDIML FRDPLPNPLS HD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc acacgaagcg agggagattc accgtggatc tgtccgtgtg tgcgctcagt agccttccta cagatactct ctctgtggaa aaagagaatg atgtgcttta ccgcctttta gaacttcttc ctgagcgcta cacgagcaaa gttttggaag agcagcgaat ccccgagggt ctcatcgtgc aaattctcat caaggcacgc gctataatag ccagtgagcc gatgcttgtc gaattagatt cccctgttta cgcctgcggt gatctacacg ggcagtacta cgacttggtt aacattttca agcggtgccc accactggga ggcaccgttt tcggaactga tgcgaagaag gcacgtaaag agaccagctc atcgatcgat gataaaacat acagttttct cttccttggc gattatgtcg accgcggcgc tcgctccgtt gaggtcgttg tgacactgct cgctctcaag attatttctc cgcggcacat gactatgctt cgcggaaacc acgaggacga acaaatcatg ctcttgtatg ggttttacga tgaatgcaag aggcgctatg acatcaaact tttcaaaatg ttcaccgatt tgttccgtat tctacctgtg gcggccctgg tcaacggctc cattttctgc gtacacggcg gtctctccgc ggagctccgc ttcgtgcgag acatcccgga cacgcgtccg tgcaacgtcc cgcactccgg catcatttgt gaccttctgt gggcagaccc cgaaacggat ctgccagctg gtgtggactg ggccccgagc tcgcgcagaa tttcatctgt gttttccgaa cgggcactgg agcggttttt agttgagaat gacattgatt tggtgtgccg cgcacatcag gtggtcgaag aaggattcca gtttttcccg gatcacaaaa aacggcattt gctcaccatc ttctcggcaa ctaactactg caacgagttc gggaatagag gcggcatttt acgtgtggat gagaagggtg tatgcagcat catcacgttg gagccaccag actttgtcca gcagcgggat atcatgcttt ttcgggatcc gcttccaaac cctctgagcc acgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat