LemaA.00063.a

histidyl-tRNA synthetase, putative

CENTER ID: LemaA.00063.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00063.a.B1.GE42535 full length 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.00063.a.B1.PW38545 full length 1 473

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.30.0630
UniProt: Q4Q7P3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTASPNAA LLAEINDLKV KLAEKEALLQ PEGVVSKKSK KKSQANMIEK EPVQGCRDFP PEDMRVRRHL FGVFHETARK FGFEEYDAPV LESEELYIRK AGEEITDQMF NFVTKGGHRV TLRPEMTPSL VRLQLAKGRS LLLPAKWYSI PQCWRYEAIS RGRRREHYQW NMDIVGVKSV AAEVELVCAA CAAMESLGLT AQDVGVKVNS RKLLQCALAG AGVPDSLFAP VCVIVDKMEK LPEEQIRAEL EDLALDGQQA DDIIRTLSLR SVDDFDKNLF DKNGALSELA EFFSQMQMYG YGDWIQFDAS VVRGLAYYTG IVFEAFDRSG EFRAIYGGGR YDNLFTLYGS PQPISCVGFG FGDCVIIELL KKKKLINSLP HKVDDVVIPF NESQRGTALK VLKQLRDKGR SADIIMDSKK VAQTFSYADR IGADRAVLIA PDETEKGMAR VKMLREGQRG EDDRGDLVPF EDL
NT Sequence
atgtcctcga cggcctcacc caatgccgcg cttctcgcgg aaatcaacga cctgaaggta aaactcgccg agaaggaggc gctgctgcag ccggaggggg tagtgtcgaa gaagtccaag aaaaagagcc aggcgaacat gatcgagaaa gagcccgtgc agggctgccg agacttccct cctgaggaca tgcgcgtgcg caggcacctc ttcggcgtct tccacgagac ggcccgcaaa ttcggcttcg aggagtacga cgccccggtt ctggagtcgg aggagcttta catccgcaaa gcaggggagg agattacaga ccaaatgttc aactttgtga cgaagggtgg ccaccgtgtc acactgcggc cggagatgac gccgtcattg gtgcgtctgc agctcgccaa gggccgctct ctgcttctgc ccgccaagtg gtactccatt ccgcagtgct ggcgctacga ggccatctcc cgcggccgtc gccgtgagca ctaccagtgg aatatggata tcgtcggcgt gaagagcgtg gccgcagagg tggagctcgt gtgcgccgcg tgtgcggcga tggagtcgct cggcctcacc gcgcaggacg ttggcgtgaa ggtgaactcg cgcaagttgc tgcagtgtgc actcgccggc gctggcgtgc cggattctct ctttgctcca gtgtgcgtga tcgtcgacaa gatggagaag ctgcctgagg aacaaatccg cgcggagtta gaggacctgg ccctggatgg ccagcaggcg gatgacatca tcaggacact tagtctcagg tctgtggacg acttcgacaa gaacctcttt gacaagaacg gcgccctctc ggagctcgcg gagttcttct cgcagatgca gatgtacggg tacggcgact ggatccagtt cgatgcgagt gttgtgcgtg gactcgccta ctacaccggg atcgttttcg aagcgtttga ccgcagtggt gagttccgcg cgatctacgg aggcggccgc tacgacaacc tcttcactct gtacggcagc ccgcagccta tctcgtgcgt cgggttcggc tttggcgact gtgtcatcat tgaactgctg aagaagaaga agctgattaa ctcgctgccg cataaggtgg atgatgtggt gattccgttc aacgagtctc agcgaggcac cgccttgaag gtattgaagc agctgcgtga caagggccgt agtgccgaca tcattatgga ctccaagaag gttgctcaga ccttcagcta cgcggatcgt atcggcgctg accgtgccgt gctgattgcg ccggacgaaa cggagaaggg catggcgcgc gtgaagatgc tgcgcgaggg gcagaggggc gaggatgacc gtggtgacct tgtgcccttc gaagacttgt ag
Details for LemaA.00063.a.B1.GE42535
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHETARKFG FEEYDAPVLE SEELYIRKAG EEITDQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELED LALDGQQADD IIRTLSLRSV DDFDKNLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAIYGGGRYD NLFTLYGSPQ PISCVGFGFG DCVIIELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DRG
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc tcgacggcct cacccaatgc cgcgcttctc gcggaaatca acgacctgaa ggtaaaactc gccgagaagg aggcgctgct gcagccggag ggggtagtgt cgaagaagtc caagaaaaag agccaggcga acatgatcga gaaagagccc gtgcagggct gccgagactt ccctcctgag gacatgcgcg tgcgcaggca cctcttcggc gtcttccacg agacggcccg caaattcggc ttcgaggagt acgacgcccc ggttctggag tcggaggagc tttacatccg caaagcaggg gaggagatta cagaccaaat gttcaacttt gtgacgaagg gtggccaccg tgtcacactg cggccggaga tgacgccgtc attggtgcgt ctgcagctcg ccaagggccg ctctctgctt ctgcccgcca agtggtactc cattccgcag tgctggcgct acgaggccat ctcccgcggc cgtcgccgtg agcactacca gtggaatatg gatatcgtcg gcgtgaagag cgtggccgca gaggtggagc tcgtgtgcgc cgcgtgtgcg gcgatggagt cgctcggcct caccgcgcag gacgttggcg tgaaggtgaa ctcgcgcaag ttgctgcagt gtgcactcgc cggcgctggc gtgccggatt ctctctttgc tccagtgtgc gtgatcgtcg acaagatgga gaagctgcct gaggaacaaa tccgcgcgga gttagaggac ctggccctgg atggccagca ggcggatgac atcatcagga cacttagtct caggtctgtg gacgacttcg acaagaacct ctttgacaag aacggcgccc tctcggagct cgcggagttc ttctcgcaga tgcagatgta cgggtacggc gactggatcc agttcgatgc gagtgttgtg cgtggactcg cctactacac cgggatcgtt ttcgaagcgt ttgaccgcag tggtgagttc cgcgcgatct acggaggcgg ccgctacgac aacctcttca ctctgtacgg cagcccgcag cctatctcgt gcgtcgggtt cggctttggc gactgtgtca tcattgaact gctgaagaag aagaagctga ttaactcgct gccgcataag gtggatgatg tggtgattcc gttcaacgag tctcagcgag gcaccgcctt gaaggtattg aagcagctgc gtgacaaggg ccgtagtgcc gacatcatta tggactccaa gaaggttgct cagaccttca gctacgcgga tcgtatcggc gctgaccgtg ccgtgctgat tgcgccggac gaaacggaga agggcatggc gcgcgtgaag atgctgcgcg aggggcagag gggcgaggat gaccgtggtg a
Expected Protein Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHETARKFG FEEYDAPVLE SEELYIRKAG EEITDQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELED LALDGQQADD IIRTLSLRSV DDFDKNLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAIYGGGRYD NLFTLYGSPQ PISCVGFGFG DCVIIELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DRGDLVPFED L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct cgacggcctc acccaatgcc gcgcttctcg cggaaatcaa cgacctgaag gtaaaactcg ccgagaagga ggcgctgctg cagccggagg gggtagtgtc gaagaagtcc aagaaaaaga gccaggcgaa catgatcgag aaagagcccg tgcagggctg ccgagacttc cctcctgagg acatgcgcgt gcgcaggcac ctcttcggcg tcttccacga gacggcccgc aaattcggct tcgaggagta cgacgccccg gttctggagt cggaggagct ttacatccgc aaagcagggg aggagattac agaccaaatg ttcaactttg tgacgaaggg tggccaccgt gtcacactgc ggccggagat gacgccgtca ttggtgcgtc tgcagctcgc caagggccgc tctctgcttc tgcccgccaa gtggtactcc attccgcagt gctggcgcta cgaggccatc tcccgcggcc gtcgccgtga gcactaccag tggaatatgg atatcgtcgg cgtgaagagc gtggccgcag aggtggagct cgtgtgcgcc gcgtgtgcgg cgatggagtc gctcggcctc accgcgcagg acgttggcgt gaaggtgaac tcgcgcaagt tgctgcagtg tgcactcgcc ggcgctggcg tgccggattc tctctttgct ccagtgtgcg tgatcgtcga caagatggag aagctgcctg aggaacaaat ccgcgcggag ttagaggacc tggccctgga tggccagcag gcggatgaca tcatcaggac acttagtctc aggtctgtgg acgacttcga caagaacctc tttgacaaga acggcgccct ctcggagctc gcggagttct tctcgcagat gcagatgtac gggtacggcg actggatcca gttcgatgcg agtgttgtgc gtggactcgc ctactacacc gggatcgttt tcgaagcgtt tgaccgcagt ggtgagttcc gcgcgatcta cggaggcggc cgctacgaca acctcttcac tctgtacggc agcccgcagc ctatctcgtg cgtcgggttc ggctttggcg actgtgtcat cattgaactg ctgaagaaga agaagctgat taactcgctg ccgcataagg tggatgatgt ggtgattccg ttcaacgagt ctcagcgagg caccgccttg aaggtattga agcagctgcg tgacaagggc cgtagtgccg acatcattat ggactccaag aaggttgctc agaccttcag ctacgcggat cgtatcggcg ctgaccgtgc cgtgctgatt gcgccggacg aaacggagaa gggcatggcg cgcgtgaaga tgctgcgcga ggggcagagg ggcgaggatg accgtggtga ccttgtgccc ttcgaagact tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LemaA.00063.a.B1.PW38545
PURIFICATION DATe: 10/15/2018
CONCENTRATION: 13.48mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 210µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHETARKFG FEEYDAPVLE SEELYIRKAG EEITDQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELED LALDGQQADD IIRTLSLRSV DDFDKNLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAIYGGGRYD NLFTLYGSPQ PISCVGFGFG DCVIIELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DRG
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc tcgacggcct cacccaatgc cgcgcttctc gcggaaatca acgacctgaa ggtaaaactc gccgagaagg aggcgctgct gcagccggag ggggtagtgt cgaagaagtc caagaaaaag agccaggcga acatgatcga gaaagagccc gtgcagggct gccgagactt ccctcctgag gacatgcgcg tgcgcaggca cctcttcggc gtcttccacg agacggcccg caaattcggc ttcgaggagt acgacgcccc ggttctggag tcggaggagc tttacatccg caaagcaggg gaggagatta cagaccaaat gttcaacttt gtgacgaagg gtggccaccg tgtcacactg cggccggaga tgacgccgtc attggtgcgt ctgcagctcg ccaagggccg ctctctgctt ctgcccgcca agtggtactc cattccgcag tgctggcgct acgaggccat ctcccgcggc cgtcgccgtg agcactacca gtggaatatg gatatcgtcg gcgtgaagag cgtggccgca gaggtggagc tcgtgtgcgc cgcgtgtgcg gcgatggagt cgctcggcct caccgcgcag gacgttggcg tgaaggtgaa ctcgcgcaag ttgctgcagt gtgcactcgc cggcgctggc gtgccggatt ctctctttgc tccagtgtgc gtgatcgtcg acaagatgga gaagctgcct gaggaacaaa tccgcgcgga gttagaggac ctggccctgg atggccagca ggcggatgac atcatcagga cacttagtct caggtctgtg gacgacttcg acaagaacct ctttgacaag aacggcgccc tctcggagct cgcggagttc ttctcgcaga tgcagatgta cgggtacggc gactggatcc agttcgatgc gagtgttgtg cgtggactcg cctactacac cgggatcgtt ttcgaagcgt ttgaccgcag tggtgagttc cgcgcgatct acggaggcgg ccgctacgac aacctcttca ctctgtacgg cagcccgcag cctatctcgt gcgtcgggtt cggctttggc gactgtgtca tcattgaact gctgaagaag aagaagctga ttaactcgct gccgcataag gtggatgatg tggtgattcc gttcaacgag tctcagcgag gcaccgcctt gaaggtattg aagcagctgc gtgacaaggg ccgtagtgcc gacatcatta tggactccaa gaaggttgct cagaccttca gctacgcgga tcgtatcggc gctgaccgtg ccgtgctgat tgcgccggac gaaacggaga agggcatggc gcgcgtgaag atgctgcgcg aggggcagag gggcgaggat gaccgtggtg a
Expressed Protein Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHETARKFG FEEYDAPVLE SEELYIRKAG EEITDQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELED LALDGQQADD IIRTLSLRSV DDFDKNLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAIYGGGRYD NLFTLYGSPQ PISCVGFGFG DCVIIELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DRGDLVPFED L
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCT CGACGGCCTC ACCCAATGCC GCGCTTCTCG CGGAAATCAA CGACCTGAAG GTAAAACTCG CCGAGAAGGA GGCGCTGCTG CAGCCGGAGG GGGTAGTGTC GAAGAAGTCC AAGAAAAAGA GCCAGGCGAA CATGATCGAG AAAGAGCCCG TGCAGGGCTG CCGAGACTTC CCTCCTGAGG ACATGCGCGT GCGCAGGCAC CTCTTCGGCG TCTTCCACGA GACGGCCCGC AAATTCGGCT TCGAGGAGTA CGACGCCCCG GTTCTGGAGT CGGAGGAGCT TTACATCCGC AAAGCAGGGG AGGAGATTAC AGACCAAATG TTCAACTTTG TGACGAAGGG TGGCCACCGT GTCACACTGC GGCCGGAGAT GACGCCGTCA TTGGTGCGTC TGCAGCTCGC CAAGGGCCGC TCTCTGCTTC TGCCCGCCAA GTGGTACTCC ATTCCGCAGT GCTGGCGCTA CGAGGCCATC TCCCGCGGCC GTCGCCGTGA GCACTACCAG TGGAATATGG ATATCGTCGG CGTGAAGAGC GTGGCCGCAG AGGTGGAGCT CGTGTGCGCC GCGTGTGCGG CGATGGAGTC GCTCGGCCTC ACCGCGCAGG ACGTTGGCGT GAAGGTGAAC TCGCGCAAGT TGCTGCAGTG TGCACTCGCC GGCGCTGGCG TGCCGGATTC TCTCTTTGCT CCAGTGTGCG TGATCGTCGA CAAGATGGAG AAGCTGCCTG AGGAACAAAT CCGCGCGGAG TTAGAGGACC TGGCCCTGGA TGGCCAGCAG GCGGATGACA TCATCAGGAC ACTTAGTCTC AGGTCTGTGG ACGACTTCGA CAAGAACCTC TTTGACAAGA ACGGCGCCCT CTCGGAGCTC GCGGAGTTCT TCTCGCAGAT GCAGATGTAC GGGTACGGCG ACTGGATCCA GTTCGATGCG AGTGTTGTGC GTGGACTCGC CTACTACACC GGGATCGTTT TCGAAGCGTT TGACCGCAGT GGTGAGTTCC GCGCGATCTA CGGAGGCGGC CGCTACGACA ACCTCTTCAC TCTGTACGGC AGCCCGCAGC CTATCTCGTG CGTCGGGTTC GGCTTTGGCG ACTGTGTCAT CATTGAACTG CTGAAGAAGA AGAAGCTGAT TAACTCGCTG CCGCATAAGG TGGATGATGT GGTGATTCCG TTCAACGAGT CTCAGCGAGG CACCGCCTTG AAGGTATTGA AGCAGCTGCG TGACAAGGGC CGTAGTGCCG ACATCATTAT GGACTCCAAG AAGGTTGCTC AGACCTTCAG CTACGCGGAT CGTATCGGCG CTGACCGTGC CGTGCTGATT GCGCCGGACG AAACGGAGAA GGGCATGGCG CGCGTGAAGA TGCTGCGCGA GGGGCAGAGG GGCGAGGATG ACCGTGGTGA CCTTGTGCCC TTCGAAGACT TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT