LemaA.00019.a
prostaglandin f synthase
| CENTER ID: | LemaA.00019.a |
| ORGANISM: | Leishmania major Friedlin |
| ASSOCIATED DISEASE: | Cutaneous leishmaniasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.00019.a.B1.GE34411 | full length | 1 | 284 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.00019.a.B1.PW35021 | full length | 1 | 284 |
Structures
4F40
DEPOSITED: 5/9/2012
DETERMINATION: XRay
CLONE: LemaA.00019.a.B1.GE34411
PROTEIN: LemaA.00019.a.B1.PW35021
4G5D
DEPOSITED: 7/17/2012
DETERMINATION: XRay
CLONE: LemaA.00019.a.B1.GE34411
PROTEIN: LemaA.00019.a.B1.PW35021
Publications by SSGCID
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
Barnes SR, Edwards TE, Fairman JW, Lorimer DD, Moen SO, Myler PJ, Nakazawa-Hewitt S, Staker BL, Sullivan A, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 609-14
PMID: 25945716; PMCID: PMC4361283
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:LmjF31.2150 |
| OrthoMCL: | OG5_126583 |
| RefSeq: | XP_001685202.1 |
| UniProt: | P22045 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGVDKAMVT LSNGVKMPQF GLGVWQSPAG EVTENAVKWA LCAGYRHIDT AAIYKNEESV GAGLRASGVP REDVFITTKL WNTEQGYEST LAAFEESRQK LGVDYIDLYL IHWPRGKDIL SKEGKKYLDS WRAFEQLYKE KKVRAIGVSN FHIHHLEDVL AMCTVTPMVN QVELHPLNNQ ADLRAFCDAK QIKVEAWSPL GQGKLLSNPI LSAIGAKYNK TAAQVILRWN IQKNLITIPK SVHRERIEEN ADIFDFELGA EDVMSIDALN TNSRYGPDPD EAQF
NT Sequence
atggctggcg ttgataaggc aatggtcacg ctgagcaacg gcgtcaagat gccgcagttc ggtctcggcg tgtggcagtc ccccgccggc gaggtaaccg agaacgctgt caagtgggcg ctgtgtgccg gctaccgcca catcgacacg gcagccatct acaagaacga ggagagcgtc ggggctgggc tgcgtgcctc tggtgtaccg cgcgaggacg tgttcatcac gacgaagctc tggaacacgg agcagggcta cgagagcacg cttgcggcct tcgaggagag tcgacagaag ctcggtgtcg actacattga tctctacctc atccactggc cgcgcggcaa agatatcctg tcgaaagagg gcaagaagta cctggattcg tggcgcgcct tcgagcagct ctacaaggag aagaaggtgc gggcgattgg agtgtcgaac tttcacatcc accatctgga ggacgtgctt gcaatgtgca ctgtgacgcc aatggtgaac caggtggagc ttcacccgtt gaacaatcag gccgatctgc gggccttctg cgacgccaag caaatcaaag ttgaggcttg gtcgccgcta gggcagggca agctgctctc caacccgatc ctctccgcaa tcggtgcaaa gtacaataag acggccgcgc aggtgatcct ccgctggaac atccagaaga atctcatcac gatccccaag tcagtccaca gggagcgtat cgaggagaac gcggacatct tcgactttga gctcggcgcc gaggacgtga tgagtatcga cgctctcaac acgaactcgc gctacggccc cgaccctgat gaggcgcagt tctaa
Details for LemaA.00019.a.B1.GE34411
| HARVESTED ON: | 2/6/2012 |
| SEQUENCED ON: | 2/21/2012 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MVTXSXGVKM PQFGLGVWQS PAGEVTENAV KWALCAGYRH IDTAAIYKNE ESVGAGLRAS GVPREDVFIT TKLWNTEQGY ESTLAAFEES RQKLGVDYID LYLIHWPRGK DILSKEGKKY LDSWRAFEQL YKEKKVRAIG VSNFHIHHLE DVLAMCTVTP MVNQVELHPL NNQADLRAFC DAKQIKVEAW SPLGQGKLLS NPILSAIGAK YNKTAAQVIL RWNIQKNLIT IPKSVHRERI EENADIFDFE LGAEDVMSID ALNTNSRYGP DPDEAQF
Validated NT Sequence
atcttactca cttagaactg cgcctcatca gggtcggggc cgtagcgcga gttcgtgttg agagcgtcga tactcatcac gtcctcggcg ccgagctcaa agtcgaagat gtccgcgttc tcctcgatac gctccctgtg gactgacttg gggatcgtga tgagattctt ctggatgttc cagcggagga tcacctgcgc ggccgtctta ttgtactttg caccgattgc ggagaggatc gggttggaga gcagcttgcc ctgccctagc ggcgaccaag cctcaacttt gatttgcttg gcgtcgcaga aggcccgcag atcggcctga ttgttcaacg ggtgaagctc cacctggttc accattggcg tcacagtgca cattgcaagc acgtcctcca gatggtggat gtgaaagttc gacactccaa tcgcccgcac cttcttctcc ttgtagagct gctcgaaggc gcgccacgaa tccaggtact tcttgccctc tttcgacagg atatctttgc cgcgcggcca gtggatgagg tagagatcaa tgtagtcgac accgagcttc tgtcgactct cctcgaaggc cgcaagcgtg ctctcgtagc cctgctccgt gttccagagc ttcgtcgtga tgaacacgtc ctcgcgcggt acaccagagg cacgcagccc agccccgacg ctctcctcgt tcttgtagat ggctgccgtg tcgatgtggc ggtagccggc acacagcgcc cacttgacag cgttctcggt tacctcgccg gcgggggact gccacacgcc gagaccgaac tgcggcatct tgacgccntt gctcancgtg accattgnct tatcaacgcc
Expected Protein Sequence
MAHHHHHHMA GVDKAMVTLS NGVKMPQFGL GVWQSPAGEV TENAVKWALC AGYRHIDTAA IYKNEESVGA GLRASGVPRE DVFITTKLWN TEQGYESTLA AFEESRQKLG VDYIDLYLIH WPRGKDILSK EGKKYLDSWR AFEQLYKEKK VRAIGVSNFH IHHLEDVLAM CTVTPMVNQV ELHPLNNQAD LRAFCDAKQI KVEAWSPLGQ GKLLSNPILS AIGAKYNKTA AQVILRWNIQ KNLITIPKSV HRERIEENAD IFDFELGAED VMSIDALNTN SRYGPDPDEA QF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg gcgttgataa ggcaatggtc acgctgagca acggcgtcaa gatgccgcag ttcggtctcg gcgtgtggca gtcccccgcc ggcgaggtaa ccgagaacgc tgtcaagtgg gcgctgtgtg ccggctaccg ccacatcgac acggcagcca tctacaagaa cgaggagagc gtcggggctg ggctgcgtgc ctctggtgta ccgcgcgagg acgtgttcat cacgacgaag ctctggaaca cggagcaggg ctacgagagc acgcttgcgg ccttcgagga gagtcgacag aagctcggtg tcgactacat tgatctctac ctcatccact ggccgcgcgg caaagatatc ctgtcgaaag agggcaagaa gtacctggat tcgtggcgcg ccttcgagca gctctacaag gagaagaagg tgcgggcgat tggagtgtcg aactttcaca tccaccatct ggaggacgtg cttgcaatgt gcactgtgac gccaatggtg aaccaggtgg agcttcaccc gttgaacaat caggccgatc tgcgggcctt ctgcgacgcc aagcaaatca aagttgaggc ttggtcgccg ctagggcagg gcaagctgct ctccaacccg atcctctccg caatcggtgc aaagtacaat aagacggccg cgcaggtgat cctccgctgg aacatccaga agaatctcat cacgatcccc aagtcagtcc acagggagcg tatcgaggag aacgcggaca tcttcgactt tgagctcggc gccgaggacg tgatgagtat cgacgctctc aacacgaact cgcgctacgg ccccgaccct gatgaggcgc agttctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LemaA.00019.a.B1.PW35021
| PURIFICATION DATe: | 3/21/2012 |
| CONCENTRATION: | 50.75mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVTXSXGVKM PQFGLGVWQS PAGEVTENAV KWALCAGYRH IDTAAIYKNE ESVGAGLRAS GVPREDVFIT TKLWNTEQGY ESTLAAFEES RQKLGVDYID LYLIHWPRGK DILSKEGKKY LDSWRAFEQL YKEKKVRAIG VSNFHIHHLE DVLAMCTVTP MVNQVELHPL NNQADLRAFC DAKQIKVEAW SPLGQGKLLS NPILSAIGAK YNKTAAQVIL RWNIQKNLIT IPKSVHRERI EENADIFDFE LGAEDVMSID ALNTNSRYGP DPDEAQF
Validated NT Sequence
atcttactca cttagaactg cgcctcatca gggtcggggc cgtagcgcga gttcgtgttg agagcgtcga tactcatcac gtcctcggcg ccgagctcaa agtcgaagat gtccgcgttc tcctcgatac gctccctgtg gactgacttg gggatcgtga tgagattctt ctggatgttc cagcggagga tcacctgcgc ggccgtctta ttgtactttg caccgattgc ggagaggatc gggttggaga gcagcttgcc ctgccctagc ggcgaccaag cctcaacttt gatttgcttg gcgtcgcaga aggcccgcag atcggcctga ttgttcaacg ggtgaagctc cacctggttc accattggcg tcacagtgca cattgcaagc acgtcctcca gatggtggat gtgaaagttc gacactccaa tcgcccgcac cttcttctcc ttgtagagct gctcgaaggc gcgccacgaa tccaggtact tcttgccctc tttcgacagg atatctttgc cgcgcggcca gtggatgagg tagagatcaa tgtagtcgac accgagcttc tgtcgactct cctcgaaggc cgcaagcgtg ctctcgtagc cctgctccgt gttccagagc ttcgtcgtga tgaacacgtc ctcgcgcggt acaccagagg cacgcagccc agccccgacg ctctcctcgt tcttgtagat ggctgccgtg tcgatgtggc ggtagccggc acacagcgcc cacttgacag cgttctcggt tacctcgccg gcgggggact gccacacgcc gagaccgaac tgcggcatct tgacgccntt gctcancgtg accattgnct tatcaacgcc
Expressed Protein Sequence
MAHHHHHHMA GVDKAMVTLS NGVKMPQFGL GVWQSPAGEV TENAVKWALC AGYRHIDTAA IYKNEESVGA GLRASGVPRE DVFITTKLWN TEQGYESTLA AFEESRQKLG VDYIDLYLIH WPRGKDILSK EGKKYLDSWR AFEQLYKEKK VRAIGVSNFH IHHLEDVLAM CTVTPMVNQV ELHPLNNQAD LRAFCDAKQI KVEAWSPLGQ GKLLSNPILS AIGAKYNKTA AQVILRWNIQ KNLITIPKSV HRERIEENAD IFDFELGAED VMSIDALNTN SRYGPDPDEA QF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG GCGTTGATAA GGCAATGGTC ACGCTGAGCA ACGGCGTCAA GATGCCGCAG TTCGGTCTCG GCGTGTGGCA GTCCCCCGCC GGCGAGGTAA CCGAGAACGC TGTCAAGTGG GCGCTGTGTG CCGGCTACCG CCACATCGAC ACGGCAGCCA TCTACAAGAA CGAGGAGAGC GTCGGGGCTG GGCTGCGTGC CTCTGGTGTA CCGCGCGAGG ACGTGTTCAT CACGACGAAG CTCTGGAACA CGGAGCAGGG CTACGAGAGC ACGCTTGCGG CCTTCGAGGA GAGTCGACAG AAGCTCGGTG TCGACTACAT TGATCTCTAC CTCATCCACT GGCCGCGCGG CAAAGATATC CTGTCGAAAG AGGGCAAGAA GTACCTGGAT TCGTGGCGCG CCTTCGAGCA GCTCTACAAG GAGAAGAAGG TGCGGGCGAT TGGAGTGTCG AACTTTCACA TCCACCATCT GGAGGACGTG CTTGCAATGT GCACTGTGAC GCCAATGGTG AACCAGGTGG AGCTTCACCC GTTGAACAAT CAGGCCGATC TGCGGGCCTT CTGCGACGCC AAGCAAATCA AAGTTGAGGC TTGGTCGCCG CTAGGGCAGG GCAAGCTGCT CTCCAACCCG ATCCTCTCCG CAATCGGTGC AAAGTACAAT AAGACGGCCG CGCAGGTGAT CCTCCGCTGG AACATCCAGA AGAATCTCAT CACGATCCCC AAGTCAGTCC ACAGGGAGCG TATCGAGGAG AACGCGGACA TCTTCGACTT TGAGCTCGGC GCCGAGGACG TGATGAGTAT CGACGCTCTC AACACGAACT CGCGCTACGG CCCCGACCCT GATGAGGCGC AGTTCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT