SSGCID: Target Page

LemaA.00010.b

quinonoid dihydropteridine reductase

CENTER ID:	LemaA.00010.b
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.00010.b.B2.GE40684	mature protein		22	229

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF34.4360

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNVLLIGAR GALGRAVANA FANGKWSIIS VDQAAAVQQG DECCAVNPAS SIEELQQAYK SAVTGLKVDA VINVAGGWAG GSVADASTAA STELMLRQSL FSSVAAAHVF STQGEKNGLL LLTGAAAAVS PTPGMIGYGT AKSAVHFLCQ SIAEDPSVLP TDASVLAILP TILDTPGNRS AMPHADRSTW TSLEDVAQQI VEWSNGSRRP ASGSLVKIVT ENSKTRFIV

NT Sequence

atgaaaaatg tactcctcat cggtgctcgc ggcgcccttg gtcgtgccgt cgcgaacgcc tttgccaacg gcaaatggtc catcatcagt gtagatcagg cagctgctgt ccagcagggc gatgagtgct gcgctgtcaa cccggcaagc tccatcgagg agctgcagca ggcctacaag tctgcggtca ccggactcaa ggttgacgca gtgatcaacg tggctggcgg ttgggccggc ggctctgtcg cggacgccag caccgctgcc tcgacagagc tgatgctgag acaatcgctc ttctcctccg tagcggcggc acatgtgttc agtacgcagg gtgaaaaaaa tggactgctt cttctcaccg gcgcagcggc tgctgtgagc cccacacccg gtatgattgg atacggcaca gcaaagtctg cagtgcactt tctctgccag tctatcgcgg aagacccatc cgtgttgccg acagatgcga gtgtcctggc tatcctcccc actatccttg acactcccgg aaatagaagt gcgatgcctc atgctgaccg ctccacgtgg acgtcgctcg aggacgtggc gcagcaaatc gtggagtgga gcaatggaag tcgtcgtcct gctagcggat cactcgttaa gatcgttaca gaaaactcga agacgcgttt tattgtgtag

Details for LemaA.00010.b.B2.GE40684

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA NGKWSIISVD QAAAVQQGDE CCAVNPASSI EELQQAYKSA VTGLKVDAVI NVAGGWAGGS VADASTAAST ELMLRQSLFS SVAAAHVFST QGEKNGLLLL TGAAAAVSPT PGMIGYGTAK SAVHFLCQSI AEDPSVLPTD ASVLAILPTI LDTPGNRSAM PHADRSTWTS LEDVAQQIVE WSNGSRRPAS GSLVKIVTEN SKTRFIV

Validated NT Sequence

atggctcacc accaccacca ccatatggcc aacggcaaat ggtccatcat cagtgtagat caggcagctg ctgtccagca gggcgatgag tgctgcgctg tcaacccggc aagctccatc gaggagctgc agcaggccta caagtctgcg gtcaccggac tcaaggttga cgcagtgatc aacgtggctg gcggttgggc cggcggctct gtcgcggacg ccagcaccgc tgcctcgaca gagctgatgc tgagacaatc gctcttctcc tccgtagcgg cggcacatgt gttcagtacg cagggtgaaa aaaatggact gcttcttctc accggcgcag cggctgctgt gagccccaca cccggtatga ttggatacgg cacagcaaag tctgcagtgc actttctctg ccagtctatc gcggaagacc catccgtgtt gccgacagat gcgagtgtcc tggctatcct ccccactatc cttgacactc ccggaaatag aagtgcgatg cctcatgctg accgctccac gtggacgtcg ctcgaggacg tggcgcagca aatcgtggag tggagcaatg gaagtcgtcg tcctgctagc ggatcactcg ttaagatcgt tacagaaaac tcgaagacgc gttttattgt gtaagtgagt aagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca acggcaaatg gtccatcatc agtgtagatc aggcagctgc tgtccagcag ggcgatgagt gctgcgctgt caacccggca agctccatcg aggagctgca gcaggcctac aagtctgcgg tcaccggact caaggttgac gcagtgatca acgtggctgg cggttgggcc ggcggctctg tcgcggacgc cagcaccgct gcctcgacag agctgatgct gagacaatcg ctcttctcct ccgtagcggc ggcacatgtg ttcagtacgc agggtgaaaa aaatggactg cttcttctca ccggcgcagc ggctgctgtg agccccacac ccggtatgat tggatacggc acagcaaagt ctgcagtgca ctttctctgc cagtctatcg cggaagaccc atccgtgttg ccgacagatg cgagtgtcct ggctatcctc cccactatcc ttgacactcc cggaaataga agtgcgatgc ctcatgctga ccgctccacg tggacgtcgc tcgaggacgt ggcgcagcaa atcgtggagt ggagcaatgg aagtcgtcgt cctgctagcg gatcactcgt taagatcgtt acagaaaact cgaagacgcg ttttattgtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat