LeinA.20232.a

kinetoplastid kinetochore protein 3, putative (kkt3)

CENTER ID: LeinA.20232.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.20232.a.B2.GE42800 PHoranyi kinase domain truncation 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.35.4110
UniProt: A4IBU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGTVDAIDY DGDRLHKVVL RFPAVRSGES EIVKEVWPCE RIGQGSFGTV YRAVSSDYPR LALKISTGKS TRLRQELDVL SRVCTKGRLL LPRFEFGALN KTADLIVIGM ELCVPSTLHD LLLSTRITSE AEMLFMAHQA VQAVSYVHAE GCIHRDIKLQ NFVFDLDGNL KLIDFGLACN SLKPPAGDVV AGTVSFMSPE MAHNALHKDR RVSVGVAADV WSLGIVLFSI FTQRNPYPAP ETPAPAAGST PGGAGAAGVT GRGDITHGAE GEKGNDLSQQ HRMNERLLRR VAAGDWQWPV GVTVSQDLKQ LVNSILVVNP EERPSVSTIL ENKLWNLRRR YPPAAVAAFL GVQDDFLLSH DEAHLMRAVE ERSAGVAASL RNSRLHSPAS ASNEDNGETD AQHSSSGSAR NGGLNTSGAT TSPARSSLKV VQRCGEGGID GAVTVQVYDV RASTRKRSKP IREISVVMAE ETAKTRRSKS ARRATGAVSA PSSRVVSRAA STEYSRRIAP PAGARLQSSA AHTCANSGDD EGEAEAEAVN RGTSTSQRHS RGMSPVRLQD ALETAGSVAA GQRDSVGHPK ALVEPSCATP PLLTSGKQQP LSEHMRPDIQ RSGSVELLED AEAPTEASAM PTSHKRAASS GKKRRDASLR QPSSLILKGS TRDLSADAPR STTTAASTQA STSLLASRTT LPLSAVNPSP SSSRQASLRR QASASAAAVS SAQGCAGHRG SSPVMKRAQR VALELGLDVI WHDEADHRRA LSAMLLIEHA WLLASFRLTI EEDQERYSIT WLAEEQEKSA AHPHRFKEVM QVMSKKYQYG FVCDMCDYEF LPTGPGEKDL HFFHCPCGRD LCPDCYTAYQ RQCTCSCCRA VHSNSCVLRE HLLLTGGTQY YSGSRKTNAA ARADAVRGSF QAAASLNEEA ESGDEASAPP EPPRRRGRPP KQDKNRSAVK QKGSRAAKDS SRRRRGAQDT LDVSVDDAHE VEQINLPRIS IAAMQQQEER SSNGSHRGGG TAAVGVAPRP QRPEDVEVKQ RPVESVPEGP WRPFARFKKD RRDEVAQQPT PEERDALLNG EWIRHFYLFP QAEPERVAAS GTWAEGEEEP YAFVYHAQPG RTGAIFLTSD FPMHSAVFSM LERQFFVVNQ VDTVEGVDST RATSLLKAKG HPELRIAFHA LQDIVAYDTN MMKQQRTPGT VSVYQAPRSA YSCNGEPFLY VRWFRFNENR TLSAFLLSNG AVQVFVNNEY ELRWFDESRK FLIRYNGVCE LVDDGTFALA PGINHLLYDS FDA
NT Sequence
atgctcggca cagtcgatgc gatcgactac gatggggacc gcctgcacaa ggtggtgctg cgattccctg ctgtgcgtag cggcgagtcg gagatcgtga aggaggtgtg gccgtgcgag cgcattggcc agggctcctt cggcacggtg taccgcgccg tcagcagcga ctatccacgt ctggccctta aaatttcaac aggcaagagt acgcggcttc gccaggagct agatgtgctg agtcgcgtgt gtacgaaagg gcggctcctg cttccgcgtt tcgagtttgg ggcgctcaac aagacagcgg acttgatcgt gatcggcatg gagctgtgtg ttccgagcac gctacacgac cttctcctca gcactcgtat caccagcgaa gcggagatgc ttttcatggc gcatcaggcg gttcaagccg tctcgtacgt gcacgcagag ggttgcatcc accgcgacat caagctgcag aactttgtct tcgacctcga tggtaacctg aagctgattg actttggcct ggcttgtaac tcgctcaagc caccggcagg cgacgtggtg gcgggcactg tgtctttcat gtctccggag atggcgcaca acgcccttca caaggacagg cgcgtgagcg tcggcgtggc tgctgatgta tggtcgctgg ggattgtcct cttttctatt ttcacgcagc ggaacccgta tccggcgccg gagacgccgg cgccggcagc gggcagcacc cccggtggcg cgggtgcagc gggcgtgacc ggacgtggag acatcacgca tggcgcggag ggcgagaagg gcaacgactt gagtcagcag caccgcatga atgagcgcct gctgcgccgc gtggcggccg gggactggca gtggcctgtc ggcgttacag tgtcacagga cctgaagcaa cttgtaaact ccatcctcgt cgtcaacccc gaggagcggc cgagcgtgag cacgatcctg gagaacaagt tgtggaactt acggcggcgc tatccacctg cagctgtggc ggcatttctc ggcgtgcagg atgacttcct gctgtctcac gatgaggcgc acctgatgcg tgcggtcgag gagcgtagtg ccggcgtggc agcgtcgttg cggaacagtc gcctacacag ccccgcctcg gcctccaacg aggacaatgg cgagaccgac gcgcagcaca gcagcagcgg cagcgcgcgc aacggtggcc taaacacatc tggcgccact acatctccag cgcgcagctc tctcaaggtg gtgcagcgct gcggcgaggg cggcattgat ggggcggtca cagtgcaggt atacgacgtg cgcgccagca cccgcaagcg cagcaagccc atccgcgaga tatccgtcgt catggcggag gagactgcca agacgcgacg atccaagtcc gcccgacggg caacgggcgc cgtgtcggct ccctcgtcgc gcgtcgtctc acgagcagcg tcgacggagt acagcaggcg aatagctcct ccggctggcg cgaggttgca gtccagtgcg gcgcatacgt gtgccaacag cggcgacgat gagggtgagg cggaggcgga ggcggtgaac cgtggcacca gcacgtcgca gcgccacagc cgcggtatgt cgcctgtgcg gctgcaggat gctctggaga ccgccggctc agtagcagcc gggcagaggg acagcgtagg ccaccccaag gcattggtgg agccgtcctg tgccacgcca cctctgctca caagcggcaa acagcagcct ctcagcgagc acatgcgccc cgacattcag cgcagcggga gtgtggagct gctggaggat gcggaggcgc caactgaagc gagcgcgatg cccacctcgc acaagagggc tgcgtcgagc ggcaagaaaa ggcgcgacgc gtctctgcgc cagccttcct cgttgatcct caaaggcagc acccgcgact tatccgctga tgccccgcgc agcacgacga cagcggcatc gacacaggcg tccacctctc tgctggcgag ccgcactaca ttgccgcttt cagccgtaaa cccatcccca tcgtcttcgc ggcaggcatc gctgaggcgg caggcatctg ctagcgccgc cgccgtctcc tccgcacagg ggtgtgcagg ccacagggga tcatcgccgg tgatgaagcg tgcgcagcgt gtcgcgctcg agcttggtct ggatgttatc tggcatgacg aggcggacca ccgccgcgcg ctttctgcga tgctgctgat cgagcatgcg tggctgctcg ccagctttcg gctgaccatt gaggaggacc aggagcgcta cagcatcacg tggctcgcag aggagcagga gaagtccgcc gcccacccac accgtttcaa agaggtgatg caggtgatga gcaagaagta tcaatacggc ttcgtttgtg acatgtgcga ctatgagttt ctcccgactg gcccagggga gaaggacttg cacttctttc actgcccatg cggccgcgac ctgtgcccag actgctacac agcgtatcag cggcagtgca cgtgctcctg ctgccgtgcg gtgcactcaa acagctgtgt gctgcgggag cacctcttgc tgactggcgg aactcagtac tacagcgggt cgagaaaaac gaacgcagcg gcgcgggctg atgctgtgcg cggctcattc caagccgctg ccagcctcaa cgaagaagcg gagagcgggg atgaggcgtc ggcaccgcct gagccgccgc gtcgtcgtgg ccggcccccc aagcaggaca agaaccgctc tgctgtaaag cagaagggca gccgagcagc caaagacagc agccgtcggc gccgtggtgc acaggacacc ctcgacgtga gtgtggacga tgcgcatgag gtggagcaga ttaacctacc tcgaatttca atcgctgcga tgcagcaaca ggaggagcgt agtagcaacg gctcgcaccg cggcggcggg actgcagccg tcggggtagc cccgcgacca caacggccag aggacgtgga ggtaaaacag cgaccggtag agagcgtccc ggagggcccg tggcggccgt tcgcgcgctt caagaaggat cgccgagatg aggtagcgca gcagccaacg ccggaggagc gcgacgcgtt gctgaatggg gagtggattc gacactttta cttgtttccg caagctgagc cggagcgcgt tgctgcctcc gggacgtggg cggaagggga ggaggagccg tacgcgttcg tctatcacgc gcagccgggc cgcaccggcg ccatttttct tacaagtgac tttccgatgc actcggcagt cttttccatg ctggagcggc agttcttcgt tgtgaatcag gtggatacgg tggagggcgt cgactccacc cgcgccacat cgctgctcaa ggcgaagggt cacccggagc tccggatcgc tttccatgcg ctgcaggaca tcgtagcgta tgacacaaac atgatgaagc agcagcgtac ccctggcacc gtctccgtgt accaggcacc tcgcagcgcc tacagctgca acggcgagcc tttcctgtac gtgcggtggt tccgcttcaa cgagaaccgc accctcagcg cctttcttct ctccaacggc gccgttcagg tcttcgtaaa caacgagtac gagctgcggt ggttcgacga gagccggaag ttcctcatcc gctacaacgg cgtgtgcgag ttggtcgacg acggcacttt tgcactggca ccaggaatca atcatctcct ctacgactcc ttcgacgcgt ag
Details for LeinA.20232.a.B2.GE42800
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 37kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 59
PERCENT COVERAGE: 51
Validated AA Sequence
MAHHHHHHML GTVDAIDYDG DRLHKVVLRF PAVRSGXXEI VKXVXXXERI GXGSFGTVYR AVSXDYPRLX LKXSXXKSTR LXQELDVLSX VCTXXRLLLP RXXVWGAQXX SGLDRXXXGA VCSEHATRPS PQXXXHQRSG DAFHGASGGS SRLVRARRGL HPPRHQAAEL CLXPXW
Validated NT Sequence
tgnnnnnnnn nnnccgctcc tnnnngttga nnnnnnnnan nnnnnnnacn nnntgcttnn nnnnctgnnn nnctgtaacg ccgacnnnnc actgnnnnnn nccggccncn nnnnnnnnnn nnnnnnnntc attcatgcgg tgctgctgac tnnngtcgnt gcccttctcg ccctccgcgc catncgnnnn gtctcnnnnn ncnnnnnngc ccgctgcacc cgcgccaccn nnggcgctgc cnnnnnncgg cgccggcgtc tccggcnnnn natacnnnnt ccgctgngtg aaaatagaaa agaggacaat cccnnnngac catannnnag cagccnnncc gacgctcacg cnnctgtcct tgtgaagggc gttgtgcgcc atctccggag acatgaaaga cacagtgccc gccaccacgt cgcctgccgg tggcttgagc gagttacaag ccaggccaaa gtcaatcagc ttcaggttac cannnnggtc naagacaaag ttctgcagct tgatgtcgcg gtggatgcaa ccctctgcgt gcacgtacga gacggcttga accgcctgat gcgccatgaa aagcatctcc gcttcgctgg tgatnnnant gctgaggaga aggtcgtgta gcgtgctcgg aacacacagc tccatnnnnn tcacgatcaa gtccgctnnn nnnttgagcg ccccaaacnn nnaaacgcgg aagcaggagc cgccnnnncg tacacacnnn actcagcaca tctagctcct ggcnaagccg cgtactcttn cnngntgaaa ntttaagggn cagncgtgga tagtcnnngc tnacggcgcg gtacaccgtg ccgaaggagc ccnggccaat gcgctcgcnn nnncacacnn ccttcacgat ctcnnnntcg ccgctacgca cagcagggaa tcgcagcacc accttgtgca ggcggtcccc atcgtagtcg atcgcatcga ctgtgccgag catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHML GTVDAIDYDG DRLHKVVLRF PAVRSGESEI VKEVWPCERI GQGSFGTVYR AVSSDYPRLA LKISTGKSTR LRQELDVLSR VCTKGRLLLP RFEFGALNKT ADLIVIGMEL CVPSTLHDLL LSTRITSEAE MLFMAHQAVQ AVSYVHAEGC IHRDIKLQNF VFDLDGNLKL IDFGLACNSL KPPAGDVVAG TVSFMSPEMA HNALHKDRRV SVGVAADVWS LGIVLFSIFT QRNPYPAPET PAPAAGSTPG GAGAAGVTGR GDITHGAEGE KGNDLSQQHR MNERLLRRVA AGDWQWPVGV TVSQDLKQLV NSILVVNPEE RPSVSTILEN KLW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg gcacagtcga tgcgatcgac tacgatgggg accgcctgca caaggtggtg ctgcgattcc ctgctgtgcg tagcggcgag tcggagatcg tgaaggaggt gtggccgtgc gagcgcattg gccagggctc cttcggcacg gtgtaccgcg ccgtcagcag cgactatcca cgtctggccc ttaaaatttc aacaggcaag agtacgcggc ttcgccagga gctagatgtg ctgagtcgcg tgtgtacgaa agggcggctc ctgcttccgc gtttcgagtt tggggcgctc aacaagacag cggacttgat cgtgatcggc atggagctgt gtgttccgag cacgctacac gaccttctcc tcagcactcg tatcaccagc gaagcggaga tgcttttcat ggcgcatcag gcggttcaag ccgtctcgta cgtgcacgca gagggttgca tccaccgcga catcaagctg cagaactttg tcttcgacct cgatggtaac ctgaagctga ttgactttgg cctggcttgt aactcgctca agccaccggc aggcgacgtg gtggcgggca ctgtgtcttt catgtctccg gagatggcgc acaacgccct tcacaaggac aggcgcgtga gcgtcggcgt ggctgctgat gtatggtcgc tggggattgt cctcttttct attttcacgc agcggaaccc gtatccggcg ccggagacgc cggcgccggc agcgggcagc acccccggtg gcgcgggtgc agcgggcgtg accggacgtg gagacatcac gcatggcgcg gagggcgaga agggcaacga cttgagtcag cagcaccgca tgaatgagcg cctgctgcgc cgcgtggcgg ccggggactg gcagtggcct gtcggcgtta cagtgtcaca ggacctgaag caacttgtaa actccatcct cgtcgtcaac cccgaggagc ggccgagcgt gagcacgatc ctggagaaca agttgtggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat