LeinA.20231.a

serine-threonine protein kinase-like protein (LUK1)

CENTER ID: LeinA.20231.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.20231.a.B2.GE43491 kinase domain 695 1070
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.04.0650
UniProt: A4HS61

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRTPSSLAP LPLSSFKQLS MNAPVQERSG TSSSNAPPAH KKQRHEGGCS GKVLDDRDAD SVRVWGVLVA QNLPSRENGL LAHAPRCRCP VEGADAPGKE SVTKLIASSS LREPEEDWLW RHVPPALRQF YFPVCSHSGS TYPCAPASAE CSEKCWAASL QQEGDKSHPS PSPRLLLPLC GTRSNNGAVR LGRLASNGIV FATNPCVSNV HCTIAYQPGS DPRRGDAAAA ATVAAEDKEA QRAAYEAAGA VAKVVAPLHR SPQPATDAPA APHPRGDATG RNAARIMLSD YSTNGCIVNG QKVGKGRSAR LRDGDVVELI NAGPRRTTDY NLSFLFLTAE AFVRWVAEQP HQASTTQGAD EDIALAEKEQ QQRQALQTAA RRRALLCRAQ WNVEAQVRRM YGHSVDEYYS LDRAHPLGQG TFGTVYRAAL RAEASASSQS PSLLDAGGGV AAGVPSSWEY VFSGDGVGEW TANPAEAAEL REAYIRGKER RAPASVLHVS ASAASTTAAE ADAITTDDAA PQVFAVKIIR KQRMLFEALQ QQQQQQHVEE GEGGTDRAPV SCIRNAATVA TPGTAGAVPG TISAADAAVI EQLLLMETQP DDLAAQRRKE WTAGVLASLQ LADLTPAALT PSSSLSAFRK SQHKPRDDDD SSDDGDAQPL SQQDREQRRR ELIRCLPEAL RRTYERELQH RRRQQREINI LLAVRHRNVT ALYEVFDQPD HLALVMEQAT GGEVWDLLQI YKRRERGGKT GARKSERGAN NHRNAGAQRL GDGKSNSTTA AATAATCDDD DDVELVSVGG PLPEFIVKII IVQVIEAVLY LHTMGIIHRD LKLENLMLQR PCDRYALNAL QLQTLVHQLR AYSHSHHASD ASTSAATTCE EDSEAVEFTN PLSVLYTVHV PRHMWPVVKI MDFGLSHVLD HLQTHPFDSA GALHGFAGTL SQMSQGVEGR SAAAASAATE AVPERVKLIY SRNDATTSCG TPIYAAPEVT NPALRPDKMG YGAAVDMYSV GVIAYALLTG RAPFPSAKNP RRPGGPPVVN YDAPLRFQRH RRRPAVPKGA TGPPASAGPL PSSRPSSSAA RPPSEVACLP PLSVVESVRV VLPKERAETS AAFSAASEQT RQWHRRAEAV AASVRCAEQK RALAEGSDGG SSATPADTLF ATTVEQLCAS LTAYADMCAS GGLEVDLDRL VYVSADAAAT GVQANASVGR VMAPDGHVAD VLLPPISALG TSFLRGLLEK YPSRRLTAYE ALRHPWLREC V
NT Sequence
atgacgcgca ctccatcatc acttgcaccc ctccctctct cctccttcaa gcagctctcg atgaacgcac cggtacagga acgcagtggc accagcagca gtaacgcgcc acctgcgcac aagaagcagc gccatgaagg tgggtgtagt ggcaaggtcc ttgacgaccg tgacgcggat agtgtgcgtg tctggggtgt cctcgtcgcg cagaacctgc cgtcgagaga aaacgggctg ctggcacacg cgccgcgttg tcgctgccct gtggaagggg cagacgcgcc ggggaaagag tcagtgacga agctgatagc ctcatcgtcc ctgcgtgaac ctgaagagga ctggttgtgg cggcacgttc caccagctct gcggcagttc tactttccgg tgtgctccca cagcggcagc acgtaccctt gcgcaccagc gtccgcagaa tgttctgaga agtgttgggc ggcctcgctg cagcaggagg gagataagtc tcacccctcg ccatcacctc gccttctcct gcccctgtgt ggcactcgca gcaacaacgg cgctgtccgt ctcggtcgcc tcgccagcaa cggcatcgtc ttcgccacga atccgtgtgt cagcaatgtg cactgcacta tcgcctacca accgggaagt gatcctcgtc gcggtgacgc tgcggcggcg gctacagtgg cggcggaaga caaggaggca cagagggcgg cctacgaggc ggccggcgcc gtggccaagg tcgtggctcc gcttcatcgc agtcctcaac ccgcaacgga tgcgccagct gcgccgcatc cacgcggcga tgcaaccggc cgcaacgccg ctcgcattat gctctctgac tacagcacaa acggatgcat cgtcaacggc cagaaggtgg gcaaaggccg ctcggcgcgt ctgcgcgacg gtgacgtggt ggagctcatc aacgccggcc ctcggcgcac gacggactac aacctctcgt ttctcttcct cacggcggag gcgtttgtgc gttgggtggc agagcagccg catcaggcga gcacgacaca gggcgctgat gaggacatcg ccttggcgga gaaggaacag cagcaacgtc aggcactgca gacagctgcg cgacggcgtg cgctgctgtg cagagcgcag tggaacgtcg aggcgcaggt gcggcgcatg tacggtcaca gcgttgacga gtactactcc cttgatcggg cacatccgct gggccaggga acgttcggca ccgtgtaccg cgccgccttg cgcgcagagg cgtcagcctc atcgcagtcg ccgtccctcc tcgacgctgg tggtggtgtc gcggctggtg tgccctcaag ctgggagtac gtcttcagcg gcgacggcgt gggtgagtgg acggcgaacc cggcagaggc ggctgagcta cgggaagcct acatacgggg gaaggagcga cgggcgccag cctcggtgct gcatgtgtcc gcgtcggcgg cctcgacgac agcagcggag gcggacgcaa tcaccacaga tgatgctgcg ccacaggtgt tcgctgtcaa gatcatccgc aagcagcgca tgttgtttga agctcttcag cagcagcagc agcagcagca tgtcgaggaa ggcgaaggcg gtacggatcg agcacctgtg tcatgcatcc gcaacgctgc caccgttgcc acccctggta ctgcgggggc agtgccaggg acgatatcgg ccgcagatgc cgccgtgatt gagcagctgt tgctcatgga gacgcagccg gacgatctgg ccgcgcagag acggaaggag tggacagcag gtgtgctcgc cagtcttcag cttgccgact taacgcccgc tgccctgacg ccgtcctcct cgctcagcgc atttcggaag tcgcagcaca agccgcgaga cgacgacgac tcgagtgatg acggggacgc acagccactg tcgcagcaag atcgtgagca acgccgtcga gagctgatcc gctgcctccc cgaggcactc cgccgcacat atgagagaga gttgcagcac cgccgccgtc agcagcgcga gatcaacatt ctcctcgccg tgcgtcaccg caacgtcacc gcactgtacg aggtgtttga ccagccagac cacctggcac tggtgatgga gcaggcgact ggaggggaag tgtgggacct gctgcagatc tacaagcgac gtgagcgcgg ggggaaaaca ggtgctcgta agagcgagcg tggcgcgaac aaccatcgta acgccggtgc gcaacggcta ggcgacggca agagcaactc gacaacggca gcggcgactg cagctacgtg cgacgacgac gacgacgttg agctcgtctc ggtcggcggt ccgctgccgg agttcattgt gaagatcatt atcgttcaag tgattgaggc agtgttgtac ctgcacacga tgggcatcat ccaccgtgac ttgaagctcg aaaatttgat gctgcagcgc ccctgcgacc gctacgcgct caacgcgctg cagctgcaga cgttggttca tcagctccgc gcctactctc actcgcacca tgcaagcgac gcgtccacat ccgccgcgac gacgtgtgag gaagacagtg aggcggtgga gttcacaaat ccgctgtcgg tgctttacac ggtgcacgta ccgcggcaca tgtggccggt cgtcaagatc atggatttcg gtctctcgca tgtgctcgac cacctgcaga cacatccgtt cgactccgca ggggcgctgc acggcttcgc cgggacgctc tcgcagatgt cacagggcgt tgaaggcagg tctgctgcag cggcatcagc agccacggag gcggtgccgg agcgggtgaa gctgatctat tcccgcaacg acgcaacgac gagctgcggc acccctatct acgcagcccc tgaggtcacc aacccggcct tgcgcccgga caagatgggg tacggtgcag cagtcgacat gtactccgtc ggcgtcatcg cgtacgcgct gctgacgggg cgggcaccgt ttccgtcagc gaaaaacccg cggcggccgg gtggcccacc tgtggtgaac tatgacgccc ccctgcgctt ccagcggcac cggcgccgcc cagcagtgcc gaaaggcgcc accggccccc cagccagtgc tgggccgctc ccatcgtcac ggccgtccag ctctgcggca cgccctcctt ctgaagtcgc gtgtctacct ccgctgtcag tggtagagtc ggtgcgcgtg gtgctgccga aagaacgtgc cgagacgtca gctgcgttct ccgccgcctc ggagcagact cggcagtggc accggcgcgc cgaggcggtg gcggcgtctg tgcggtgtgc ggagcagaag cgtgcccttg ctgagggcag cgatggcggc agcagcgcga cgccggctga cacgctcttt gccaccaccg tggagcagct ctgcgcatca ctcaccgcgt acgccgacat gtgtgcgtcg ggtgggctgg aggtggacct cgatcgcctc gtgtacgtct ccgcggacgc tgccgcgacc ggtgtgcagg caaatgcgtc cgtcggtcgc gtgatggcgc ctgatgggca cgtcgccgat gtgttgctac cccccatctc ggcgctcggc acctccttcc ttcgcgggct tctggagaag tacccttctc ggcggctgac ggcttacgag gcattgcggc atccgtggtt gcgcgagtgc gtgtga
Details for LeinA.20231.a.B2.GE43491
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ REINILLAVR HRNVTALYEV FDQPDHLALV MEQATGGEVW DLLQIYKRRE RGGKTGARKS ERGANNHRNA GAQRLGDGKS NSTTAAATAA TCDDDDDVEL VSVGGPLPEF IVKIIIVQVI EAVLYLHTMG IIHRDLKLEN LMLQRPCDRY ALNALQLQTL VHQLRAYSHS HHASDASTSA ATTCEDDSEA VEFTNPLSVL YTVHVPRHMW PVVKIMDFGL SHVLDHLQTH PFDSAGALHG FAGTLSQMSQ GVEGRSAAAA SAATEAVPER VKLIYSRNDA TTSCGTPIYA APEVTNPALR PDKMGYGAAV DMYSVGVIAY ALLTGRAPFP SAKNPRRPGG PPVVNYDAPL RFQRHRRRPA VPKGATGP
Validated NT Sequence
tggggggccg gtggcgcctt tcggcactgc tgggcggcgc cggtgccgct ggaagcgcag gggggcgtca tagttcacca caggtgggcc acccggccgc cgcgggtttt tcgctgacgg aaacggtgcc cgccccgtca gcagcgcgta cgcgatgacg ccgacggagt acatgtcgac tgctgcaccg taccccatct tgtccgggcg caaggccggg ttggtgacct caggggctgc gtagataggg gtgccgcagc tcgtcgttgc gtcgttgcgg gaatagatca gcttcacccg ctccggcacc gcctccgtgg ctgctgatgc cgctgcagca gacctgcctt caacgccctg tgacatctgc gagagcgtcc cggcgaagcc gtgcagcgcc cctgcggagt cgaacggatg tgtctgcagg tggtcgagca catgcgagag accgaaatcc atgatcttga cgaccggcca catgtgccgc ggtacgtgca ccgtgtaaag caccgacagc ggatttgtga actccaccgc ctcactgtcg tcctcacacg tcgtcgcggc ggatgtggac gcgtcgcttg catggtgcga gtgagagtag gcgcggagct gatgaaccaa cgtctgcagc tgcagcgcgt tgagcgcgta gcggtcgcag gggcgctgca gcatcaaatt ttcgagcttc aagtcacggt ggatgatgcc catcgtatgc aggtacaaca ctgcctcaat cacttgaacg ataatgatct tcacaatgaa ctccggcagc ggaccgccga ccgagacgag ctcaacgtcg tcgtcgtcgt cgcacgtagc tgcagtcgcc gctgccgttg tcgagttgct cttgccgtcg ccaagccgtt gcgcaccggc gttacgatgg ttgttcgcgc cacgctcgct cttacgagca cctgttttcc ccccgcgctc acgtcgcttg tagatctgca gcaggtccca cacttcccct ccagtcgcct gctccatcac cagtgccagg tggtctggct ggtcaaacac ctcgtacagt gcggtgacgt tgcggtgacg cacggcgagg agaatgttga tctcgcgctg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHQR EINILLAVRH RNVTALYEVF DQPDHLALVM EQATGGEVWD LLQIYKRRER GGKTGARKSE RGANNHRNAG AQRLGDGKSN STTAAATAAT CDDDDDVELV SVGGPLPEFI VKIIIVQVIE AVLYLHTMGI IHRDLKLENL MLQRPCDRYA LNALQLQTLV HQLRAYSHSH HASDASTSAA TTCEEDSEAV EFTNPLSVLY TVHVPRHMWP VVKIMDFGLS HVLDHLQTHP FDSAGALHGF AGTLSQMSQG VEGRSAAAAS AATEAVPERV KLIYSRNDAT TSCGTPIYAA PEVTNPALRP DKMGYGAAVD MYSVGVIAYA LLTGRAPFPS AKNPRRPGGP PVVNYDAPLR FQRHRRRPAV PKGATGPPAS AGPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagcgcg agatcaacat tctcctcgcc gtgcgtcacc gcaacgtcac cgcactgtac gaggtgtttg accagccaga ccacctggca ctggtgatgg agcaggcgac tggaggggaa gtgtgggacc tgctgcagat ctacaagcga cgtgagcgcg gggggaaaac aggtgctcgt aagagcgagc gtggcgcgaa caaccatcgt aacgccggtg cgcaacggct aggcgacggc aagagcaact cgacaacggc agcggcgact gcagctacgt gcgacgacga cgacgacgtt gagctcgtct cggtcggcgg tccgctgccg gagttcattg tgaagatcat tatcgttcaa gtgattgagg cagtgttgta cctgcacacg atgggcatca tccaccgtga cttgaagctc gaaaatttga tgctgcagcg cccctgcgac cgctacgcgc tcaacgcgct gcagctgcag acgttggttc atcagctccg cgcctactct cactcgcacc atgcaagcga cgcgtccaca tccgccgcga cgacgtgtga ggaagacagt gaggcggtgg agttcacaaa tccgctgtcg gtgctttaca cggtgcacgt accgcggcac atgtggccgg tcgtcaagat catggatttc ggtctctcgc atgtgctcga ccacctgcag acacatccgt tcgactccgc aggggcgctg cacggcttcg ccgggacgct ctcgcagatg tcacagggcg ttgaaggcag gtctgctgca gcggcatcag cagccacgga ggcggtgccg gagcgggtga agctgatcta ttcccgcaac gacgcaacga cgagctgcgg cacccctatc tacgcagccc ctgaggtcac caacccggcc ttgcgcccgg acaagatggg gtacggtgca gcagtcgaca tgtactccgt cggcgtcatc gcgtacgcgc tgctgacggg gcgggcaccg tttccgtcag cgaaaaaccc gcggcggccg ggtggcccac ctgtggtgaa ctatgacgcc cccctgcgct tccagcggca ccggcgccgc ccagcagtgc cgaaaggcgc caccggcccc ccagccagtg ctgggccgct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat