LeinA.20229.a

mitogen activated kinase-like protein (LUK8)

CENTER ID: LeinA.20229.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.20229.a.B2.GE43490 kinase domain 689 977
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.36.3870
UniProt: A4ID28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGASVRIPI EKYDIHDVFF SLSVLRKLPE YTRELEEGSS ETLYRIIHSS ICAARWYYRR YSRYGEFVHL LNTYAECIAV KSSNSALRVA ELLAARTSQL HTPQLWQRLP LPIAASLLFA VICCTATVIL IGIGEDCCRK ADVAALILSC VSVLCLCALF PLIYASQRRR GYKGQERLVD RMVRMACDNA QSQNSFYNPL SDRIELTERV IVEDADAFLV DADIAHRNRR AIAMLLTEAT EDEGSVVLRS RGLPPAMIGL NAIYRHTIII MTDFDGKVVM WSDGAATCFG FSARDVEGNN IISLLYGERS VELYATMTEA AEKNLDLSEK VLTVAHMGLG TVSISATVVV SREAKSNQPV GFTLIGSVRS DELSQTQAVL HSFFVAELSQ LSVKDSQFRQ IVDCLRWKNL RDLSALARDW RSAHIRQLLS EVIKGRQRYV SVEVDPKVTE LPPILCDTVG ISAVLNRAFE LFCEKIRVRV EQKRTTSAVY QLIVVYRHDM SGLNRNTLID ITRSANDLGG IVVDSPGTLK LLLPFMVKDE AIQALRPQGA AAQKPIGHDP LIVLLLEKNA VHRHNISASI WSCGHSLRLV ENVRKALQAI EGSTDLGCAI IDVDVKGSDR VIEALLAKHI YIIETSEALD GGAKRGDALL KKPISNEALR KELEKATYKC EEAKRAGEEL LKRREVFGKV RNSPWTQGRL LGRGGHAAVY EATSTLTGGK MAVKIIRVSG NFEERIDEFM NEIGILCKLT HPNIIHYFYC ERTETTLNLF MAMADQGTVA DLIKRCPRLP ENHVATITKQ LLQAVNYLHE CGIIHRDIKP GNMLISQGQL KLSDFGTATT NVREGTVGTI SYMAPEVVDG KPSGKESDIW SIGCVVCECL QIKRSGDGLL GYGAPEEYPS DVSAQAIDFI KACMQRNPSE RATTGTLLLH DFIVHLDQEV SQLAEVLPEA APNEEGAKTP KARSSFSDST VISWSFD
NT Sequence
atgagcggcg cctcagtgag aatccccatc gaaaaatatg atatacacga tgttttcttt tccctgagtg tcctgaggaa gctaccggag tacacaaggg agctggagga gggctcttca gagacattgt accgaattat tcatagctcg atttgcgccg cccgttggta ctatcgccgc tactcccggt acggagaatt cgttcattta ctcaacacat atgcggagtg tattgctgtg aaatcgtcca attctgcact ccgagtcgcg gagctacttg cagctcgcac ttcccaactt cataccccgc agctgtggca gcggcttcca cttccgattg ccgcttctct tctttttgcc gtgatttgct gcactgcgac ggtgatactt atcggtattg gagaagattg ctgtcgaaaa gcggatgttg ctgcgctgat actgagctgt gtgagtgtct tgtgcctatg tgccctgttt ccactcattt acgcgtccca aaggcggcgc ggatacaaag ggcaggagcg gcttgttgac cgcatggtgc gaatggcctg tgacaacgct caaagccaaa attcgttcta caacccgctg tcggaccgaa ttgaactcac cgagcgagtg atagtcgagg acgcagatgc gttccttgtt gacgccgaca ttgcccatcg taaccgaagg gctatcgcga tgctcttaac agaggccacc gaagacgagg ggagtgttgt cttacggagc agaggccttc ctcctgctat gatcggactt aacgccatct atagacatac gatcattatc atgacggact ttgatgggaa agtcgtaatg tggagcgatg gtgcggctac ctgcttcggc tttagtgcac gagacgtcga ggggaacaat ataatttcgc ttctctatgg cgaacggtcg gtggagctct acgccacaat gactgaagcc gctgagaaaa acttggacct ctccgaaaag gtcctcaccg tagcacatat gggcttgggc actgtctcta tcagcgcaac agtggtcgtc agccgagagg ccaaatcgaa tcagcccgtg gggttcaccc tcatcggatc tgtacgctcc gatgagctgt cgcagacgca ggcggtgctg cactcctttt ttgtggcaga actgtcgcag ctgtctgtca aggacagtca atttcgccaa attgtggact gcttgcggtg gaaaaacctg cgtgacctct ccgcgctggc gagggactgg agaagcgcgc acattcggca gctgctgtcc gaggtcatca aaggtaggca gcgctacgtg agcgtcgagg tcgatccaaa ggtaaccgaa ttgccgccta tcctctgcga cacggtgggt atcagtgctg tgctaaatcg cgctttcgag ctgttctgcg agaaaattcg cgtccgcgtt gagcaaaaac gcacgactag tgcggtgtac cagctcattg tagtttaccg tcatgacatg tctgggctca accgaaatac cttgattgac atcacccgat ctgcaaatga ccttggtggc attgtagttg actcccctgg tacgctgaag ctgttgttgc ccttcatggt gaaggatgag gcaattcaag ccttgaggcc ccaaggtgcc gctgcacaga agccgatcgg acacgacccc ctcattgtgc tactcttgga gaagaatgct gtgcaccgtc acaacatatc tgcctccatc tggagctgcg gccattctct tcggctggtt gagaacgttc gcaaggcgct acaggccatc gaaggatcga ccgacctcgg ctgcgctatt atcgacgtcg acgtgaaagg ctctgaccgc gtgatcgagg cactgctggc gaagcacatc tacataatcg aaacctctga agcgctagac ggcggtgcaa aacgcggtga tgcccttctg aagaagccga tctcgaatga ggccctccgc aaggagctag agaaggctac ctacaagtgc gaggaggcga agcgtgccgg tgaggagctg ctgaagcggc gagaggtgtt cggaaaggtg cgcaacagtc catggacaca gggccgtctg ctcgggcgtg gcgggcacgc tgccgtgtac gaggcgactt caaccttgac gggcggaaaa atggctgtca agatcatccg cgtgtctggc aactttgaag agcgcatcga cgagtttatg aatgagattg ggattctgtg taagttgaca catccgaaca tcattcacta cttttactgc gagcgaacgg agacaaccct caacctcttc atggcgatgg ctgaccaggg tacggtggcc gaccttatca agcgctgccc acgactgccc gagaaccacg tcgccaccat cacgaaacaa cttctgcaag cggtcaacta cttgcacgag tgtgggatta tccaccgcga cattaagcca ggcaacatgc tcatttcgca agggcagctg aagctgtccg acttcggcac agccaccacg aacgttcgag agggcacagt tggcaccatc agctacatgg ccccagaggt cgttgatgga aagccgagcg gtaaggaaag cgacatctgg tcgattggct gcgttgtctg tgagtgtttg cagatcaagc gctctggtga tggcttgctt gggtatggcg caccagaaga gtacccgagt gatgtatccg ctcaggcgat cgacttcatc aaggcctgca tgcagagaaa tccctcagag cgtgcaacaa ctggcacgct gctgctgcac gacttcattg tacacctcga ccaagaggtg tcacagctgg ctgaagtact accggaggcg gcgccaaacg aagagggggc aaagacacct aaagcccgca gcagtttcag tgactccacc gtcatcagct ggtccttcga ctag
Details for LeinA.20229.a.B2.GE43490
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK VRNSPWTQGR LLGRGGHAAV YEATSTLTGG KMAVKIIRVS GNFEEHIDEF MNEIGILCKL THPNIIHYFY CERTETTLNL FMAMADQGTV ADLIKRCPRL PENHVATITK QLLQAVNYLH ECGIIHRDIK PGNMLISQGQ LKLSDFGTAT TNVREGTVGT ISYMAPEVVD GKPSGKESDI WSIGCVVCEC LQIKRSGDGL LGYGAPEEYP SDVSAQAIDF IKACMQRNPS ERATTGTLLL HDFIVHLDQE VSQLAEVLPE AAPNEEGAKT PKARSSFSDS TVISWSFD
Validated NT Sequence
atcttactca cttagtcgaa ggaccagctg atgacggtgg agtcactgaa actgctgcgg gctttaggtg tctttgcccc ctcttcgttt ggcgccgcct ccggtagtac ttcagccagc tgtgacacct cttggtcgag gtgtacaatg aagtcgtgca gcagcagcgt gccagttgtt gcacgctctg agggatttct ctgcatgcag gccttgatga agtcgatcgc ctgagcggat acatcactcg ggtactcttc tggtgcgcca tacccaagca agccatcacc agagcgcttg atctgcaaac actcacagac aacgcagcca atcgaccaga tgtcgctttc cttaccgctc ggctttccat caacgacctc tggggccatg tagctgatgg tgccaactgt gccctctcga acgttcgtgg tggctgtgcc gaagtcggac agcttcagct gcccttgcga aatgagcatg ttgcctggct taatgtcgcg gtggataatc ccacactcgt gcaagtagtt gaccgcttgc agaagttgtt tcgtgatggt ggcgacgtgg ttctcgggca gtcgtgggca gcgcttgata aggtcggcca ccgtaccctg gtcagccatc gccatgaaga ggttgagggt tgtctccgtt cgctcgcagt aaaagtagtg aatgatgttc ggatgtgtca acttacacag aatcccaatc tcattcataa actcgtcgat gtgctcttca aagttgccag acacgcggat gatcttgaca gccatttttc cgcccgtcaa ggtggaagtc gcctcgtaca cggcagcgtg cccgccacgc ccgagcagac gaccctgtgt ccatggactg ttgcgcacct tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHKV RNSPWTQGRL LGRGGHAAVY EATSTLTGGK MAVKIIRVSG NFEERIDEFM NEIGILCKLT HPNIIHYFYC ERTETTLNLF MAMADQGTVA DLIKRCPRLP ENHVATITKQ LLQAVNYLHE CGIIHRDIKP GNMLISQGQL KLSDFGTATT NVREGTVGTI SYMAPEVVDG KPSGKESDIW SIGCVVCECL QIKRSGDGLL GYGAPEEYPS DVSAQAIDFI KACMQRNPSE RATTGTLLLH DFIVHLDQEV SQLAEVLPEA APNEEGAKTP KARSSFSDST VISWSFD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaggtgc gcaacagtcc atggacacag ggccgtctgc tcgggcgtgg cgggcacgct gccgtgtacg aggcgacttc aaccttgacg ggcggaaaaa tggctgtcaa gatcatccgc gtgtctggca actttgaaga gcgcatcgac gagtttatga atgagattgg gattctgtgt aagttgacac atccgaacat cattcactac ttttactgcg agcgaacgga gacaaccctc aacctcttca tggcgatggc tgaccagggt acggtggccg accttatcaa gcgctgccca cgactgcccg agaaccacgt cgccaccatc acgaaacaac ttctgcaagc ggtcaactac ttgcacgagt gtgggattat ccaccgcgac attaagccag gcaacatgct catttcgcaa gggcagctga agctgtccga cttcggcaca gccaccacga acgttcgaga gggcacagtt ggcaccatca gctacatggc cccagaggtc gttgatggaa agccgagcgg taaggaaagc gacatctggt cgattggctg cgttgtctgt gagtgtttgc agatcaagcg ctctggtgat ggcttgcttg ggtatggcgc accagaagag tacccgagtg atgtatccgc tcaggcgatc gacttcatca aggcctgcat gcagagaaat ccctcagagc gtgcaacaac tggcacgctg ctgctgcacg acttcattgt acacctcgac caagaggtgt cacagctggc tgaagtacta ccggaggcgg cgccaaacga agagggggca aagacaccta aagcccgcag cagtttcagt gactccaccg tcatcagctg gtccttcgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat