LeinA.20227.a

protein kinase, putative (OPH8)

CENTER ID: LeinA.20227.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.20227.a.B2.GE42797 PHoranyi kinase domain truncation 449 752
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.25.1580
UniProt: A4I1J8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPSSKRHAY EPLPQQQQQQ HPTNPPPAYQ HHRVSTGTGR ESPALPQLSP NSMSAVTNIA EPSSSPALIS TSAASSNLVP IFQGREDSAA ASRRGGDGNS EVHPHPAEVS ETYPGASTAA AQSMALAVVP FTGTSGVGVG VSAPQRCDPR VGGADQEGKN TGVGGDDVGY YSGPSPYGKL APLSLSTERV CPHCLRPYDV FDDGEGVFDP ATFFRVDATT RSSSIQPSPS HPKLLPPPSH EGDEQQKQQP STSPRLYTEG AGDGGAAAGQ LLCSRSSTAA GLDDSSSDGT NRSGNYITAV HPPAAAFTSH YFRALPPPSF IIAAGARGAS APLAIEDYRP SPLIGSPRDE KVEARQPFAL APALASDNGG GGGNSSGTLV CKVAAVPHYS YVWPITCTGM RSQVRLLQQG DSSEKTAAEM EGGKEESPSC ESGSPVCSPT AATPSPNNGY YRHYFKELRQ LGRGTYGGVY LCRHVMCGVN LGEFALKKIP VGDKVTYLQS VLREVRILEE VRRHPNVVEY KHSWVEEAQL ADFGPPVRCL FILMEYASAG SLDTYLERYG NNLSTLAVWY FFLSSVAGTA HLHEKHILHR DLKPQNLLLA ATKDRPPRVL VSDFGTAALL DDILYDRSGG TGTLEYMAPE LLETTASPRG ANERYVNHHT MASDVWSLGM VLHYLAFDGA LPERHEDGSV NLDKARHSAN ARPPEMLRLL EAMLQLDPAK RPRCCDILAS PMVQSIMRIF NKDDHSQWDL SIQRQQQLSA TSPSAILPLE RPPRPPVPAP APRSAPLNAL EAHSIDDSSS PRGARSRNAI AAISTSSQGR GVRADSTNLE DKSNTLQSSR VELLSASTLV DLSTAMARAG SSSSSNGSPL AMKLPPPRPS TVISQHGRQQ QQRAHVVAGL PKTLASSNTF QFSDSPSPRR VNVGVQTNPV VIVEKQQRHA
NT Sequence
atgaacccgt cgtcaaaacg gcacgcgtac gagccgctgc cgcagcagca gcagcagcag caccccacaa acccgccgcc tgcctatcag catcaccgag taagcacagg cactggtcgc gaaagccctg cccttccaca gctctcgccg aactcgatgt ctgcggtgac caacatcgcc gagccctcct caagccccgc cctcatctcc acctcggcag cgtcgtcgaa tctggtgccc atcttccaag ggcgggagga ttctgcagca gcttcaagac gtggcggcga cggcaattct gaagtgcatc ctcatcccgc cgaggtaagc gaaacctacc ctggggcgtc caccgcggcg gcgcagtcca tggcgctggc ggtggtgccc ttcacgggca ctagcggcgt cggcgtcggc gtcagcgcac cgcaacgttg tgacccgaga gtgggcggtg ccgaccaaga aggcaagaac acgggtgttg gtggtgatga cgttggctac tactctgggc cctccccgta tggcaagctg gcccctttgt cgctgagcac ggaacgagtg tgtccgcatt gccttcggcc gtacgacgtc ttcgacgacg gcgaaggcgt cttcgacccg gcgacgttct tccgcgtgga tgcgacgacg cggtcatcgt cgatacagcc ctcaccaagc catccgaaac tgctcccgcc gccctcgcat gagggggatg aacagcagaa gcagcaacca tcgacgtccc cgcgcttata cactgagggc gcaggggatg gtggcgctgc tgctgggcag ctgttgtgca gcaggagcag cacggccgct gggctcgatg atagcagcag tgatggcacc aaccgcagcg gcaactacat cacggccgta catccaccag ctgctgcatt cacctcgcac tactttcgtg cgctgccccc accctcgttc atcatagcgg cgggagcccg cggtgcatcg gcaccgcttg ccattgagga ctaccgtcca tcgccgctaa tcggtagccc gagagacgag aaggtggaag cgagacagcc gtttgcactg gcacccgcgc ttgccagtga caacggtggt ggaggtggta actcgagtgg gacgctggtg tgcaaagtag ctgccgtgcc gcactactcc tacgtgtggc ccatcacctg cacagggatg cgctcgcagg tgcgactgct gcagcagggt gacagcagcg agaagacggc ggcagagatg gagggcggga aagaggagtc gccgtcgtgc gagagcggct cgcccgtctg ctcccccacc gctgcgacgc cgtcgccgaa caatggctac taccgtcatt acttcaagga gctccgtcag ctgggtcgcg gcacctacgg aggagtctac ctctgtcgcc acgttatgtg tggcgttaac ctcggcgagt tcgctcttaa gaagattcca gtcggcgaca aggtcaccta cctgcagtcg gtgctgcgtg aggtgcgcat tctcgaggag gtgcgacggc accccaacgt ggtcgagtac aagcacagct gggtggagga ggcacagctc gccgactttg gtccgcccgt gcgctgtctc ttcatcttga tggaatacgc gtccgctggc tcgcttgaca cgtatctgga gcgctacggc aacaatctct ccaccctcgc cgtgtggtac ttcttcctca gctccgtggc cggcacggcg caccttcacg agaagcacat cttgcaccgc gacctaaagc cacagaacct gttgctggcc gcgacgaagg accgtccgcc acgggtgctg gtcagcgact ttggcacggc ggcgctgctg gatgacatct tgtacgaccg gtccggtggc acgggcacgc tcgagtacat ggcgccggag ctcctcgaga cgaccgcctc tccacgcggc gccaacgagc ggtacgtgaa ccatcacacg atggcctccg acgtgtggtc gctggggatg gtgctgcact acctcgcctt cgacggcgcg ctgccggagc gacacgagga cggcagtgtc aaccttgaca aggcacgcca ctcggccaac gccaggccgc ctgaaatgct cagactgctg gaggccatgc tgcagcttga tcccgcaaag cggccgcgct gctgcgacat cctcgcatct ccgatggtgc agtccatcat gcgcatcttc aacaaagatg accatagcca gtgggacctc tccatccagc ggcagcagca gctctcggct acctcgccgt ctgcgattct cccgttggag cgtccccctc gcccaccggt gccggcgcca gcgcccagaa gtgcaccact caatgctctg gaggcccaca gcatcgatga cagcagctcg ccccgcggcg ctcgcagccg caacgctatc gccgccatct ccacctcttc acagggacgc ggcgtgcggg ccgacagtac gaatctggag gacaagagca acacacttca aagcagccgt gtcgagctcc tctcagcgtc gacgttggtg gatctgtcaa cggcgatggc gcgtgccggg agcagcagca gcagcaacgg tagccccttg gcaatgaagt tgcctccgcc gcgcccatct actgtgatct cccagcatgg ccgacagcag cagcaacgcg cgcatgtggt ggccgggtta ccaaagactc tagcctcctc caacaccttc cagttttcag actcgccatc gccgcgcagg gtcaatgtcg gcgtgcaaac caatccggtg gtgatagtgg agaagcagca acggcacgca taa
Details for LeinA.20227.a.B2.GE42797
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGY YRHYFKELRQ LGRGTYGGVY LCRHVMCGVN LGEFALKKIP VGDKVTYLQS VLREVRILEE VRRHPNVVEY KHSWVEEAQL ADFGPPVRCL FILMEYASAG SLDTYLERYG NNLSTLAVWY FFLSSVAGTA HLHEKHILHR DLKPQNLLLA ATKDRPPRVL VSDFGTAALL DDILYDRSGG TGTLEYMAPE LLETTASPRG ANERYVNHHT MASDVWSLGM VLHYLAFDGA LPERHEDGSV NLDKARHSAN ARPPEMLRLL EAMLQLDPAK RPRCCDILAS PMVQSIMRIF NKDDHSQWDL SI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctact accgtcatta cttcaaggag ctccgtcagc tgggtcgcgg cacctacgga ggagtctacc tctgtcgcca cgttatgtgt ggcgttaacc tcggcgagtt cgctcttaag aagattccag tcggcgacaa ggtcacctac ctgcagtcgg tgctgcgtga ggtgcgcatt ctcgaggagg tgcgacggca ccccaacgtg gtcgagtaca agcacagctg ggtggaggag gcacagctcg ccgactttgg tccgcccgtg cgctgtctct tcatcttgat ggaatacgcg tccgctggct cgcttgacac gtatctggag cgctacggca acaatctctc caccctcgcc gtgtggtact tcttcctcag ctccgtggcc ggcacggcgc accttcacga gaagcacatc ttgcaccgcg acctaaagcc acagaacctg ttgctggccg cgacgaagga ccgtccgcca cgggtgctgg tcagcgactt tggcacggcg gcgctgctgg atgacatctt gtacgaccgg tccggtggca cgggcacgct cgagtacatg gcgccggagc tcctcgagac gaccgcctct ccacgcggcg ccaacgagcg gtacgtgaac catcacacga tggcctccga cgtgtggtcg ctggggatgg tgctgcacta cctcgccttc gacggcgcgc tgccggagcg acacgaggac ggcagtgtca accttgacaa ggcacgccac tcggccaacg ccaggccgcc tgaaatgctc agactgctgg aggccatgct gcagcttgat cccgcaaagc ggccgcgctg ctgcgacatc ctcgcatctc cgatggtgca gtccatcatg cgcatcttca acaaagatga ccatagccag tgggacctct ccatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat