LeinA.20226.a

Repressor of differentiation kinase 1, putative (RDK1)

CENTER ID: LeinA.20226.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.20226.a.B2.GE42796 PHoranyi kinase domain truncation 1098 1363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.32.0860
UniProt: A4I7P7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRNATIPAY IQSSPNHSNH NNMSLSLSLQ KQKDANADGE DYADGRRILM HNPLKGHLFS STSDSSLHSS IGQPEMIAAA SSSVQKGSSG AGNRLDTALG FCGEETAQGL VAEQVEGPCD EKHVNLIEVR AIDSDDDPSS LEEQEHQVCC TIPSLPAVAF IMCFGAIIVG LLGFLPFYFV GVQSNAHTTE YLLSEAMKGV ASITQNSLAM LPAFVHIVTF NYIKRHNTTL EETNLPQDPD QLLVSLLTVL TRFNEAISYL RFVYEGGLYT NAGYTDPKPT DANQTRKIYG GYSRTHNTVP MLEMYNESVT AVEPPNVLGY FDFHREVTLP EGAMNQIVKT WVADPQNNTR WLLASDNTTP TYFNFVMPFT VNGSLGYFAA GSSSDTIIEE TTTLVSFLRK NGRIFLVDAT RNIVVGNSWN QSVQSRTEDS AIGATIYTPT RIYEITEPII LAAMQVVNES GSLTEVLKDK DTALITFRYK GNNAFLNLTK VKDSYGLDLV LGVVVVRADF EKAFSTARTA AFTVTVLVVV IAAVVAVVIA WFVVREMKRL IPQLIRASNL EVSRGDGKDH RVQGWLAYIT EIRGIHEAFV RVERSLREMR TFVPAAVMTL APSDGSHSDA DLADGKTRRV RTRFNANIVK DNTNEFSRVD VAMVLVDVHR AMDTTEACFI MDIISQRAEE YTGYIESVSS SSFFVNFGTQ SQNPLVVSKI CRFALEVYQS VPDFMRNRVV CFAVRMPFLV GTCGARHSKA RVVFDTQRML DISKVLWDIG CHVASTTDTL SHFATSSAQI PWYRIDCVRF PDEVSQVTLC ELRDPRSSLQ DNEDMPKKMG DGLSKMCKGE YKQALMIFDS AHSENVQLKR LRKICIDRIA SGDTSKYIHL IQDVYALDAT PSNHEDMAVG GGAAMYDYDA GSPAGVATSS GEVCDPSNEP PLLACSSFEN GAGMPAPVLS QPAAHASPPV APHTTPICHS KTSPTPSVTT GGEAAEPSHL TERAVTAAMG PREFPPCGAS QVVGASGAGP GLPPFHPRTP GATTTAASVP GSSPPWGARD DNASEAPSFL TNAFVFDSSM ASADAVGATV QAADEPQVLR DVNQHEWQVS LYPIGSGAFS FIYLGMSDDG VQVAIKRIPR LHRGIKEEEM VSEVCTCAKL RHPNIVPYIS CCVTQSYLAI IMEYMPGGSL HDIIDNFGKL PRTVVRRFML DIVNGLAYLH ESTTHGDVKP HNILLGVDGV CKLSDFGSAS DRLTEACCVN EDRLMRGTAV YISPEGARNL PLTSASDIYS LGISFLEMVL GRLPWKWADP ESTDELPLRH DHEFVQCLTA NAIVVDIPDD LDNDMRELAL ASCAENPEKR PTAQELLSFA FLI
NT Sequence
atgatacgga acgccacgat cccggcgtac atccagtcca gcccgaatca cagcaaccac aacaatatga gcttgtcgct gagcctgcag aagcagaagg acgcgaacgc cgacggtgag gactacgccg acggccgacg catcttgatg cacaacccgc tcaagggcca tctcttcagc agcacctcgg actcctcgct tcacagctcc atcggccagc cggagatgat cgcggcggcg tccagcagcg tgcagaaggg gagcagcggc gccggcaacc gtctcgacac agcgctcggc ttttgcggtg aggagacggc gcaggggctg gttgctgagc aagtcgaggg cccgtgtgat gagaagcacg tgaacctgat tgaggtgcgc gccatcgact cggacgacga tccatcctcg cttgaggagc aggagcacca agtttgctgc accattccca gtcttcccgc cgttgctttt atcatgtgct ttggcgccat cattgtgggg ctgttgggtt tccttccgtt ctacttcgtc ggcgtccagt ccaacgcaca cacgacagag tatctgctga gtgaggccat gaagggcgta gcctccatca cgcagaacag cctggcgatg cttcccgcgt ttgtgcacat tgtgacgttc aactacatca agcgtcataa cacgacactg gaggagacga acttgcccca agacccggat cagctgctgg tatcgctcct cacggttctc actcgcttca acgaggccat ttcgtatctg cggttcgtct acgaaggcgg tctctacacg aatgccggtt acacagaccc gaagccaacc gatgcaaacc agacccgaaa gatctacggc ggttacagcc gcacgcacaa cacggtcccc atgctcgaaa tgtacaatga gagcgtcacc gcggttgaac cgcccaacgt attgggttac ttcgacttcc accgcgaagt cacgctccct gaaggcgcca tgaaccagat tgtgaaaacg tgggtggcgg acccgcagaa caacacccgc tggctactgg cctctgataa caccactccg acgtacttca acttcgtgat gccctttacc gtaaacggct cccttggcta cttcgcggcc ggctcctcgt cggacacgat catcgaagaa acaaccaccc tcgtaagctt tctgcgcaag aacggccgca tctttctcgt ggatgccaca cgcaacattg ttgtcggtaa cagctggaac caatcagtgc agagtagaac ggaggacagc gcgatcggcg caaccatcta caccccgact cgcatctacg aaatcaccga gcccatcatt cttgctgcca tgcaggtggt caacgagagc ggtagcttga cggaggtgtt gaaggacaag gacacggccc tcatcacctt tcggtacaag ggcaacaacg cgtttctgaa cctcacaaag gtgaaggact cgtacgggct ggacctcgtt cttggcgtcg tggtcgtgcg ggctgacttc gagaaggctt tctccaccgc gcgcacggcg gccttcacgg tgacggttct ggtggttgtg atcgccgctg tcgtcgcggt ggtcatcgcg tggtttgtcg tgcgggagat gaagcggctc atcccgcagc tcatccgcgc gtcgaacctt gaggtgtccc gcggcgacgg caaggatcac cgcgtgcagg gctggctggc gtacattacg gagattcgcg gcattcacga ggccttcgtg cgcgtcgagc ggagtctgcg agagatgcgc acgtttgtgc cggcggccgt catgactctg gcgccgtccg acggctctca cagcgacgcg gatcttgctg acggcaagac gcgccgtgtg cgtacccgct ttaacgctaa cattgtcaag gacaacacga acgagttctc acgagtggat gtggcgatgg tgctggtgga tgtgcaccgc gccatggaca cgactgaggc gtgctttatt atggatatca tctcgcaacg cgccgaagag tacactggct acatcgagtc cgtcagttcc agcagcttct tcgtcaattt cggcactcag tcacagaacc cgctcgtggt gtcgaagatt tgccgctttg cgctcgaggt gtaccaatcc gtgccggact tcatgcgcaa ccgcgtcgtc tgcttcgctg tccgcatgcc gtttcttgtc ggcacgtgcg gtgcacggca cagcaaggcc cgcgtggtct ttgacacgca gcggatgctg gacatttcaa aggtgctgtg ggacatcggc tgccacgtcg cctccacgac agacacactc tcgcacttcg ccacgagcag cgcgcagatc ccgtggtacc ggatcgactg cgtgcgcttt ccggacgagg tttcgcaggt gacactgtgc gagctgcgtg acccgcgctc gtcgctgcag gataatgaag acatgccaaa gaagatgggc gacggcctct cgaaaatgtg caagggcgag tacaagcagg cgcttatgat cttcgactcc gcgcacagcg agaacgtgca gctcaagcgg ctgcgcaaga tctgcattga ccgcatcgcc tctggcgaca caagcaagta catccatctc atccaggacg tctacgcgct ggacgccacc cccagtaacc acgaggacat ggcggtgggc ggcggcgctg cgatgtacga ctacgacgcc ggcagccctg ccggcgtggc gacgagcagc ggcgaggtgt gcgatccatc gaacgagcca ccattgcttg catgcagctc cttcgagaat ggtgcgggga tgccggcacc agtgctatcg cagcccgcag ctcacgcctc gcctcctgtt gcgccccaca ccacgccaat ctgccactcg aagacgtcgc cgacgccgtc cgtgacgacc ggcggtgagg cggcggagcc ctcgcacctc accgagcggg ctgtgacggc cgccatgggc ccccgcgagt tccctccatg cggagcatcg caggtggttg gagccagcgg cgcgggtcct ggcctccctc ccttccatcc acgaacccct ggcgctacga cgacggcggc cagcgtgcct ggaagctccc cgccctgggg cgcgcgcgac gacaacgcga gcgaggcgcc gtcgtttctc acgaacgcct tcgtcttcga ctcctccatg gcctccgcgg acgctgttgg tgcgaccgtg caggccgccg acgagccgca ggtgctgcgg gacgtgaacc agcatgagtg gcaggtctcc ctctacccga tcggcagcgg cgccttcagc ttcatctacc tgggcatgtc agacgatggc gtgcaagtgg cgatcaagcg cattccccgg ctgcaccgcg gcatcaagga ggaagagatg gtgtccgagg tatgcacgtg cgccaaactg cgacatccca acattgttcc ctacatctcc tgctgcgtaa cccagtcgta cctggcaatc atcatggaat acatgccagg gggctcgctg catgacatca tcgacaactt tggcaagctg cctcgcaccg tggtgcgccg gttcatgctc gacatcgtga acggcctcgc gtacctgcat gagtccacca cgcacggtga cgtgaagccg cacaacattc tgcttggcgt ggacggcgtc tgcaagctct ccgacttcgg ctccgcctcg gacaggctca cggaggcgtg ctgcgtcaac gaggaccgcc tgatgcgcgg cacggccgtg tacatctcac ccgagggcgc gcgcaacctt ccactgacct ccgcctctga catctactcg ctcggtatct cgttcttgga gatggtgctt ggccgcctgc cgtggaagtg ggccgatccc gagagtacag acgagctccc gctgcgtcac gaccacgagt ttgtgcagtg cctcaccgcc aatgccattg tcgtcgacat tcccgacgac ctggacaacg acatgcgcga gttggcgctg gcgagctgcg ctgagaaccc ggaaaaacga ccgacagccc aagaactgct ctccttcgcc tttttgattt ga
Details for LeinA.20226.a.B2.GE42796
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ VSLYPIGSGA FSFIYLGMSD DGVQVAIKRI PRLHRGIKEE EMVSEVCTCA KLRHPNIVPY ISCCVTQSYL AIIMEYMPGG SLHDIIDNFG KLPRTVVRRF MLDIVNGLAY LHESTTHGDV KPHNILLGVD GVCKLSDFGS ASDRLTEACC VNEDRLMRGT AVYISPEGAR NLPLTSASDI YSLGISFLEM VLGRLSWKWA DPESTDELPL RHDHEFVQCL TANAIVVDIP DDLDNDMREL ALASCAENPE KRPTAXXXXX FAFLI
Validated NT Sequence
ttactcactt aaatcaaaaa ggcgaannnn nnnnnnnnnn gggctgtcgg tcgtttttcc gggttctcag cgcagctcgc cagcgccaac tcgcgcatgt cgttgtccag gtcgtcggga atgtcgacga caatggcatt ggcggtgagg cactgcacaa actcgtggtc gtgacgcagc gggagctcgt ctgtactctc gggatcggcc cacttccacg acaggcggcc aagcaccatc tccaagaacg agataccgag cgagtagatg tcagaggcgg aggtcagtgg aaggttgcgc gcgccctcgg gtgagatgta cacggccgtg ccgcgcatca ggcggtcctc gttgacgcag cacgcctccg tgagcctgtc cgaggcggag ccgaagtcgg agagcttgca gacgccgtcc acgccaagca gaatgttgtg cggcttcacg tcaccgtgcg tggtggactc atgcaggtac gcgaggccgt tcacgatgtc gagcatgaac cggcgcacca cggtgcgagg cagcttgcca aagttgtcga tgatgtcatg cagcgagccc cctggcatgt attccatgat gattgccagg tacgactggg ttacgcagca ggagatgtag ggaacaatgt tgggatgtcg cagtttggcg cacgtgcata cctcggacac catctcttcc tccttgatgc cgcggtgcag ccggggaatg cgcttgatcg ccacttgcac gccatcgtct gacatgccca ggtagatgaa gctgaaggcg ccgctgccga tcgggtagag ggagacctgc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHQV SLYPIGSGAF SFIYLGMSDD GVQVAIKRIP RLHRGIKEEE MVSEVCTCAK LRHPNIVPYI SCCVTQSYLA IIMEYMPGGS LHDIIDNFGK LPRTVVRRFM LDIVNGLAYL HESTTHGDVK PHNILLGVDG VCKLSDFGSA SDRLTEACCV NEDRLMRGTA VYISPEGARN LPLTSASDIY SLGISFLEMV LGRLPWKWAD PESTDELPLR HDHEFVQCLT ANAIVVDIPD DLDNDMRELA LASCAENPEK RPTAQELLSF AFLI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaggtct ccctctaccc gatcggcagc ggcgccttca gcttcatcta cctgggcatg tcagacgatg gcgtgcaagt ggcgatcaag cgcattcccc ggctgcaccg cggcatcaag gaggaagaga tggtgtccga ggtatgcacg tgcgccaaac tgcgacatcc caacattgtt ccctacatct cctgctgcgt aacccagtcg tacctggcaa tcatcatgga atacatgcca gggggctcgc tgcatgacat catcgacaac tttggcaagc tgcctcgcac cgtggtgcgc cggttcatgc tcgacatcgt gaacggcctc gcgtacctgc atgagtccac cacgcacggt gacgtgaagc cgcacaacat tctgcttggc gtggacggcg tctgcaagct ctccgacttc ggctccgcct cggacaggct cacggaggcg tgctgcgtca acgaggaccg cctgatgcgc ggcacggccg tgtacatctc acccgagggc gcgcgcaacc ttccactgac ctccgcctct gacatctact cgctcggtat ctcgttcttg gagatggtgc ttggccgcct gccgtggaag tgggccgatc ccgagagtac agacgagctc ccgctgcgtc acgaccacga gtttgtgcag tgcctcaccg ccaatgccat tgtcgtcgac attcccgacg acctggacaa cgacatgcgc gagttggcgc tggcgagctg cgctgagaac ccggaaaaac gaccgacagc ccaagaactg ctctccttcg cctttttgat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat