LeinA.19676.a

Putative tRNA binding domain containing protein, putative

CENTER ID: LeinA.19676.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.19676.a.B1.GE42532 full length 1 415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.22.0350
UniProt: A4HZS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFVINSSSI LAPALQHALE SVLSVPALCI EMCAEGPHRV TVDGKTFRGL QPFLQALRRT AVTPEQKAFL GEDEEQVSLM NQWVGAAAVL DADVARAVAE PATSVAKALY SDVERILVAT SNAASHYLTG SERATMADVL LYAAAFNHSA HTEVLPTTMK WAVHAQQDAY LAPIRSSAVK TAENHSAGKA HGATRAEVSY VKPSEEEILR RRAEKEKAKA EKAAAVAAAK ASASPSARAP EGKKATKTQL DSTSLCVRVG QLTNLRRHPG ADRLYVEDMV LGDETRTIVS GLVGNYVIEE LEGTQCLVVC NMKPKSLMGV TSQGMVLCAK RDAEVKLIRP PACAKPGDRI LFGASYDAAV AAAAAPEPIS GNKMTEVLSH LHTNADGVLC WKEEPARHPS GAAVAIADMA NCPVS
NT Sequence
atgcagtttg tgatcaactc ctcgtccatt cttgcgccgg cgttgcagca tgctctcgag agcgtcttga gtgtgccggc gctgtgcatc gagatgtgtg ccgagggtcc ccaccgcgtt acagtagatg ggaagacctt ccgcgggctc cagccgtttc tgcaggcgct gcgccgcacc gccgtgacac cggagcagaa ggccttcctc ggggaggacg aggagcaggt atcgctgatg aaccagtggg ttggcgcggc ggctgtgcta gacgccgacg tcgcgcgcgc cgttgccgaa cctgccacct cggttgccaa ggctctctac agtgatgtgg agcgcattct tgtggcgacg tcgaacgcag cttcgcacta cctcaccggt agcgagcgcg ccacaatggc cgacgtgctc ctgtacgccg ccgccttcaa ccactcggcc cacacggagg tgctgccaac aaccatgaag tgggctgtgc acgcacagca ggacgcctac ctggccccca ttcgctcgtc ggctgtgaag acggcggaga accatagcgc tggcaaggct cacggcgcga ccagggcgga ggtctcctac gttaagccgt ccgaggagga gattttgcgg cgccgtgcgg agaaagagaa ggcaaaggcg gagaaggctg ctgctgtcgc cgcggcgaaa gcctcagcta gccccagcgc ccgcgcacca gagggcaaga aggccactaa gacgcagctg gattctacga gcctgtgcgt gcgtgtaggg cagctgacaa acctgcgccg tcaccctggc gccgaccggc tctacgtcga ggacatggtg ctcggtgatg agacacgcac cattgtgagc gggctggtgg ggaactacgt gatcgaggag ctggagggca cgcagtgcct cgtcgtgtgc aacatgaagc caaagtcact tatgggtgtc acttcgcagg ggatggtgct gtgcgccaag agggacgctg aagtgaagct catccgccct ccggcgtgcg ccaagccagg cgaccgcatc ctattcggcg cctcgtatga cgctgctgtc gccgcggctg ccgctccgga gccgatatcg ggcaataaga tgaccgaggt tctctcccac ctgcacacaa acgccgacgg cgtcctttgc tggaaggagg agcctgcgcg gcacccgagc ggtgcggcgg tggccattgc ggacatggca aactgccctg tgagctga
Details for LeinA.19676.a.B1.GE42532
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ FVINSSSILA PALQHALESV LSVPALCIEM CAEGPHRVTV DGKTFRGLQP FLQALRRTAV TPEQKAFLGE DEEQVSLVNQ WVGAAAVLDA DVARAVAEPA TSVAKALYSD VERILVATSN AASHYLTGSE RATMADVLLY AAAFNHSAHT EVLPTTMKWA VHAQQDAYLA PIRSSAVKTA ENHSAGKAHG ATRAEVSYVK PSEEEILRRR AEKEKAKAEK AAAVAAAKAS ASPSARAPEG KKATKTQLDS TSLCVRVGQL TNLRRHPGAD RLYVEDMVLG DETRTIVSGL VGNYVIEELE GTQCLVVCNM KPKSLMGVTS QGMVLCAKRD AEVKLIRPPA CAKPGDRILF GASYDAAVAA AAAPEPISGN KMTEVLSHLH TNADGVLCWK EEPARHPSGA AVAIA
Validated NT Sequence
atggctcacc accaccacca ccatatgcag tttgtgatca actcctcgtc cattcttgcg ccggcgttgc agcatgctct cgagagcgtc ttgagtgtgc cggcgctgtg catcgagatg tgtgccgagg gtccccaccg cgttacagta gatgggaaga ccttccgcgg gctccagccg tttctgcagg cgctgcgccg caccgccgtg acaccggagc agaaggcctt cctcggggag gacgaggagc aggtatcgct ggtgaaccag tgggttggcg cggcggctgt gctagacgcc gacgtcgcgc gcgccgttgc cgaacctgcc acctcggttg ccaaggctct ctacagtgat gtggagcgca ttcttgtggc gacgtcgaac gcagcttcgc actacctcac cggtagcgag cgcgccacaa tggccgacgt gctcctgtac gccgccgcct tcaaccactc ggcccacacg gaggtgctgc caacaaccat gaagtgggct gtgcacgcac agcaggacgc ctacctggcc cccattcgct cgtcggctgt gaagacggcg gagaaccata gcgctggcaa ggctcacggc gcgaccaggg cggaggtctc ctacgttaag ccgtccgagg aggagatttt gcggcgccgt gcggagaaag agaaggcaaa ggcggagaag gctgctgctg tcgccgcggc gaaagcctca gctagcccca gcgcccgcgc accagagggc aagaaggcca ctaagacgca gctggattct acgagcctgt gcgtgcgtgt ggggcagctg acaaacctgc gccgtcaccc tggcgccgac cggctctacg tcgaggacat ggtgctcggt gatgagacac gcaccattgt gagcgggctg gtggggaact acgtgatcga ggagctggag ggcacgcagt gcctcgtcgt gtgcaacatg aagccaaagt cacttatggg tgtcacttcg caggggatgg tgctgtgcgc caagagggac gctgaagtga agctcatccg ccctccggcg tgcgccaagc caggcgaccg catcctattc ggcgcctcgt atgacgctgc tgtcgccgcg gctgccgctc cggagccgat atcgggcaat aagatgaccg aggttctctc ccacctgcac acaaacgccg acggcgtcct ttgctggaag gaggagcctg cgcggcaccc gagcggtgcg gcggtggcca ttgcggac
Expected Protein Sequence
MAHHHHHHMQ FVINSSSILA PALQHALESV LSVPALCIEM CAEGPHRVTV DGKTFRGLQP FLQALRRTAV TPEQKAFLGE DEEQVSLMNQ WVGAAAVLDA DVARAVAEPA TSVAKALYSD VERILVATSN AASHYLTGSE RATMADVLLY AAAFNHSAHT EVLPTTMKWA VHAQQDAYLA PIRSSAVKTA ENHSAGKAHG ATRAEVSYVK PSEEEILRRR AEKEKAKAEK AAAVAAAKAS ASPSARAPEG KKATKTQLDS TSLCVRVGQL TNLRRHPGAD RLYVEDMVLG DETRTIVSGL VGNYVIEELE GTQCLVVCNM KPKSLMGVTS QGMVLCAKRD AEVKLIRPPA CAKPGDRILF GASYDAAVAA AAAPEPISGN KMTEVLSHLH TNADGVLCWK EEPARHPSGA AVAIADMANC PVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagt ttgtgatcaa ctcctcgtcc attcttgcgc cggcgttgca gcatgctctc gagagcgtct tgagtgtgcc ggcgctgtgc atcgagatgt gtgccgaggg tccccaccgc gttacagtag atgggaagac cttccgcggg ctccagccgt ttctgcaggc gctgcgccgc accgccgtga caccggagca gaaggccttc ctcggggagg acgaggagca ggtatcgctg atgaaccagt gggttggcgc ggcggctgtg ctagacgccg acgtcgcgcg cgccgttgcc gaacctgcca cctcggttgc caaggctctc tacagtgatg tggagcgcat tcttgtggcg acgtcgaacg cagcttcgca ctacctcacc ggtagcgagc gcgccacaat ggccgacgtg ctcctgtacg ccgccgcctt caaccactcg gcccacacgg aggtgctgcc aacaaccatg aagtgggctg tgcacgcaca gcaggacgcc tacctggccc ccattcgctc gtcggctgtg aagacggcgg agaaccatag cgctggcaag gctcacggcg cgaccagggc ggaggtctcc tacgttaagc cgtccgagga ggagattttg cggcgccgtg cggagaaaga gaaggcaaag gcggagaagg ctgctgctgt cgccgcggcg aaagcctcag ctagccccag cgcccgcgca ccagagggca agaaggccac taagacgcag ctggattcta cgagcctgtg cgtgcgtgta gggcagctga caaacctgcg ccgtcaccct ggcgccgacc ggctctacgt cgaggacatg gtgctcggtg atgagacacg caccattgtg agcgggctgg tggggaacta cgtgatcgag gagctggagg gcacgcagtg cctcgtcgtg tgcaacatga agccaaagtc acttatgggt gtcacttcgc aggggatggt gctgtgcgcc aagagggacg ctgaagtgaa gctcatccgc cctccggcgt gcgccaagcc aggcgaccgc atcctattcg gcgcctcgta tgacgctgct gtcgccgcgg ctgccgctcc ggagccgata tcgggcaata agatgaccga ggttctctcc cacctgcaca caaacgccga cggcgtcctt tgctggaagg aggagcctgc gcggcacccg agcggtgcgg cggtggccat tgcggacatg gcaaactgcc ctgtgagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat