LeinA.19530.a

J-binding protein 1 (JBP1)

CENTER ID:	LeinA.19530.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.19530.a.B2.GE41472	C-term domain		569	809
LeinA.19530.a.B3.GE41473	Tet-JBP oxygenase		59	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79424

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LinJ.09.1560
UniProt:	A4HU70

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEPDPKKVKL DIFDFPTARE TRTPEEVAES YAEAVKSHPF YDNVHSAIDF YDSGTIKDGR GQIIGVVLRE ALPKYAASMA SELLASAAVR TSLRSMMFGG ESPLSGIAGY FDYRGSPVEL KSRKTSFTYE HEAAWPAVFP VVDYVSELYR HVAPERWKAQ NDAIPDVVRI HGTPFSTLTI NSRFRTASHT DVGDFDGGYS CIACLDGQFK GLALAFDDFG INVLMQPRDV MIFDSHHFHS NTEVELSFSG EDWKRLTCVF YYRAALGEPA SYAEYRRRLE KSKQDTSFTP VVSNVRVKEN GTNLNRPSPV YPIFLSPFWV PMVAHCLQHC ASEAQCVHDA MTADGSRLAE VMFGEPLSTS DGIPLRGEEE KLKANSDSAS RPLSRLGGFS ETNLMVSTAV EKKKYLNSEF LSHFISAQLL DMWKQARGKW LELVGREWTH MLALNPERKD FLWRNQSEMN SAFFDLCEVG KQVMLGLLGK EAALPKEEQA FWTMYAVHLN AACAEELNMP HVAMSLRKLN VKLKDFNFGG TRYFKDMPPE EQKRRMERKQ RIEEARRHGM SSGAHEKRAN WLTNDSFDYQ TEDCVFDYAQ HKWVPPALHA KEITKNVRSG ELPTREGVVR VLVVLPDPQS KVDCVDCKLE VSETVRCSCE WERLMSSPAV HRVLAAAQRN LQLPDSVTHD NIEIRFAFHS RLPTDMCDFV VLQHVLSCIP DDVLASAYIR RSAALCSGCV FVVETDVQCR QYYTLKCSVR CDYDAVAPLF FQQLHRVSYG TKAARVRTKG ELESLIPTVC CARYKLQGSP LNTTVHVVAP APPR

NT Sequence

atggagcctg acccaaagaa ggtcaaacta gacatctttg actttccgac cgccagggag acacggacgc cggaggaagt ggcagagagc tacgcggaag ccgtcaagtc acaccccttt tacgacaacg tgcactctgc gatcgacttt tacgatagcg gtaccatcaa agatggtcgc gggcaaatca ttggtgttgt gctgcgagaa gccctaccaa agtacgcggc atccatggcg tcggagctgc tcgcatccgc tgccgtgcgc acctcgcttc ggagcatgat gtttggcgga gagtcgccct tgagcggtat tgctggctac ttcgactacc ggggctcacc ggtggagctg aagtcacgca aaacgtcctt cacatatgaa cacgaggcag cgtggccggc ggtcttccct gtcgtcgatt acgtcagcga gctctacagg catgtcgcgc cggagcggtg gaaagcacaa aacgacgcca ttccggatgt tgtgcgtatt cacggaaccc cgttctccac gcttaccatc aactcccgct tccgcacagc ctctcatacc gacgtcggcg attttgacgg tggctacagc tgcattgctt gccttgacgg gcagttcaaa gggctggccc ttgctttcga cgacttcggc attaacgtgc tcatgcagcc gcgcgatgtg atgatattcg actcgcacca cttccactca aacacggagg tggagctgtc gttctcaggg gaggactgga agcgacttac ctgcgtattc tactaccggg ccgccctcgg tgagccagca agctacgccg agtaccggcg ccggttggag aagtccaagc aggacaccag cttcactcca gtggtgagca acgtgagggt gaaggagaat ggcacaaacc tgaaccgtcc atcccctgtg taccctatct ttctctcgcc gttttgggta ccgatggtgg cgcattgcct gcagcactgc gcatcggagg cacaatgcgt tcacgacgcc atgacggcgg atgggagtcg gctggctgag gttatgtttg gggaaccgct gtcaacctct gacggcatcc cactgcgtgg agaggaggag aagctgaagg ccaacagtga cagcgcgtcc aggccgctct cgcgtctggg tgggttcagc gagacgaatc ttatggtgtc cacggcagta gaaaagaaga agtacctcaa cagcgagttc ctctcccact tcatcagcgc gcagctgctg gacatgtgga agcaggcacg cggcaagtgg ttggagctgg taggcaggga atggacgcac atgctggccc tcaaccccga gcgcaaagac ttcttgtgga ggaaccagtc cgagatgaac tcggcttttt tcgacctctg cgaggtgggg aagcaagtta tgctcggcct tctgggcaag gaggcggctc tgccaaagga agagcaggct ttctggacca tgtacgccgt gcacctgaat gcggcgtgcg cggaggagct aaacatgccg cacgtcgcca tgtcgcttcg caagctcaac gtgaagctga aggacttcaa ctttggcggc acgcgatact tcaaagacat gcctcccgag gagcagaagc gcagaatgga gcgcaagcag cgcattgagg aggcacgccg ccacggcatg tcgtccggcg cacacgaaaa gcgagcgaac tggctgacca acgactcatt cgactatcaa actgaggact gcgtgtttga ttacgcccaa cacaagtggg tgccgcctgc gcttcacgca aaggagataa cgaagaacgt gcgcagtggc gagctgccaa ctagggaagg agtggtgcga gtgctcgttg tgctgcccga cccgcaaagc aaggtggatt gtgtggactg caagctggag gtgtcggaga cggtgagatg ttcatgcgag tgggagcgtt tgatgagcag cccggctgtg catcgtgtcc tagccgctgc gcagcgcaac ctgcagctgc ctgacagcgt cacgcatgac aatatcgaga tccgctttgc cttccatagc aggctgccta cggacatgtg cgactttgtg gtgctgcagc atgtcttgag ctgcatccca gacgacgtgc tggcctcggc gtatatcagg cgctctgcag cgctctgttc cggctgtgta tttgtggtgg agacggacgt gcaatgccga caatactaca ccctcaagtg ttccgttcgc tgcgactacg acgcggtcgc accattattc ttccagcagc tgcaccgggt gagttacggg acgaaagcgg cacgcgtgcg caccaaagga gaactggagt ctcttatccc cacggtgtgc tgtgcgcgtt acaagctgca aggctcgccg ctgaacacga ccgtccacgt cgtggctccc gctccgcccc gctga

Details for LeinA.19530.a.B2.GE41472

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA NWLTNDSFDY QTEDCVVDYA QHKWVPPALH AKEITKNVRN GELPTREGVV RVLVVLPDPQ SKVDCVDCKL EVSETVRCSC EWERLMSSPA VHRVLAAAQR NLQLPDSVTH DNIEIRFAFH SRLPTDMCDF VVLQHVLSCI PDDVLASAYI RRSAALCSGC VFVVETDVQC RQYYTLKCSV RCDYDAVAPL FFQQLHRVSY GTKAARVRTK GELESLIPTV CCARYKLQGS PLNTTVHVVA

Validated NT Sequence

atggctcacc accaccacca ccatatggcg aactggctga ccaacgactc attcgactat caaactgagg actgcgtggt tgattacgcc caacacaagt gggtgccgcc tgcgcttcac gcaaaggaga taacgaagaa cgtgcgcaat ggcgagctgc caactaggga aggagtggtg cgagtgctcg ttgtgctgcc cgacccgcaa agcaaggtgg attgtgtgga ctgcaagctg gaggtgtcgg agacggtgag atgttcatgc gagtgggagc gtttgatgag cagcccggct gtgcatcgtg tcctagccgc tgcgcagcgc aacctgcagc ttcctgacag cgtcacgcat gacaatatcg agatccgctt tgccttccat agcaggctgc ctacggacat gtgcgacttt gtggtgctgc agcatgtctt gagctgcatc ccagacgacg tgctggcctc ggcgtatatc aggcgctctg cagcgctctg ttccggctgt gtatttgtgg tggagacgga cgtgcaatgc cgacaatact acaccctcaa gtgttccgtt cgctgcgact acgacgcggt cgcaccatta ttcttccagc agctgcaccg ggtgagttac gggacgaaag cggcacgcgt gcgcaccaaa ggagaactgg agtctcttat ccccacggtg tgctgtgcgc gttacaagct gcaaggttcg ccgctgaaca cgaccgtcca cgtcgtggct taagtgag

Expected Protein Sequence

MAHHHHHHAN WLTNDSFDYQ TEDCVFDYAQ HKWVPPALHA KEITKNVRSG ELPTREGVVR VLVVLPDPQS KVDCVDCKLE VSETVRCSCE WERLMSSPAV HRVLAAAQRN LQLPDSVTHD NIEIRFAFHS RLPTDMCDFV VLQHVLSCIP DDVLASAYIR RSAALCSGCV FVVETDVQCR QYYTLKCSVR CDYDAVAPLF FQQLHRVSYG TKAARVRTKG ELESLIPTVC CARYKLQGSP LNTTVHVVA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgaact ggctgaccaa cgactcattc gactatcaaa ctgaggactg cgtgtttgat tacgcccaac acaagtgggt gccgcctgcg cttcacgcaa aggagataac gaagaacgtg cgcagtggcg agctgccaac tagggaagga gtggtgcgag tgctcgttgt gctgcccgac ccgcaaagca aggtggattg tgtggactgc aagctggagg tgtcggagac ggtgagatgt tcatgcgagt gggagcgttt gatgagcagc ccggctgtgc atcgtgtcct agccgctgcg cagcgcaacc tgcagctgcc tgacagcgtc acgcatgaca atatcgagat ccgctttgcc ttccatagca ggctgcctac ggacatgtgc gactttgtgg tgctgcagca tgtcttgagc tgcatcccag acgacgtgct ggcctcggcg tatatcaggc gctctgcagc gctctgttcc ggctgtgtat ttgtggtgga gacggacgtg caatgccgac aatactacac cctcaagtgt tccgttcgct gcgactacga cgcggtcgca ccattattct tccagcagct gcaccgggtg agttacggga cgaaagcggc acgcgtgcgc accaaaggag aactggagtc tcttatcccc acggtgtgct gtgcgcgtta caagctgcaa ggctcgccgc tgaacacgac cgtccacgtc gtggcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LeinA.19530.a.B3.GE41473

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG RGQIIGVVLR EALPKYAASM ASELLASAAV RTSLRSMMFG GESPLSGIAG YFDYRGSPVE LKSRKTSFTY EHEAAWPAVF PVVDYVSELY RHVAPERWKA QNDAIPDVVR IHGTPFSTLT INSRFRTASH TDVGDFDGGY SCIACLDGQF KGLALAFDDF GINVLMQPRD VMIFDSHHFH SNTEVELSFS GEDWKRLTCV FYYRAALGEP ASYAEYRRXX EKSKQD

Validated NT Sequence

atggctcacc accaccacca ccatatgggt cgcgggcaaa tcattggtgt tgtgctgcga gaagccctac caaagtacgc ggcatccatg gcgtcggagc tgctcgcatc cgctgccgtg cgcacctcgc ttcggagcat gatgtttggc ggagagtcgc ccttgagcgg tattgctggc tacttcgact accggggctc accggtggag ctgaagtcac gcaaaacgtc cttcacatat gaacacgagg cagcgtggcc ggcggtcttc cctgtcgtcg attacgtcag cgagctctac aggcatgtcg cgccggagcg gtggaaagca caaaacgacg ccattccgga tgttgtgcgt attcacggaa ccccgttctc cacgcttacc atcaactccc gcttccgcac agcctctcat accgacgtcg gcgattttga cggtggctac agctgcattg cttgccttga cgggcagttc aaagggctgg cccttgcttt cgacgacttc ggcattaacg tgctcatgca gccgcgcgat gtgatgatat tcgactcgca ccacttccac tcaaacacgg aggtggagct gtcgttctca ggggaggact ggaagcgact tacctgcgta ttctactacc gggccgccct cggtgagcca gcaagctacg ccgagtaccg gcgccnnnnn gagaagtcca agcaggacta agtgagtaa

Expected Protein Sequence

MAHHHHHHGR GQIIGVVLRE ALPKYAASMA SELLASAAVR TSLRSMMFGG ESPLSGIAGY FDYRGSPVEL KSRKTSFTYE HEAAWPAVFP VVDYVSELYR HVAPERWKAQ NDAIPDVVRI HGTPFSTLTI NSRFRTASHT DVGDFDGGYS CIACLDGQFK GLALAFDDFG INVLMQPRDV MIFDSHHFHS NTEVELSFSG EDWKRLTCVF YYRAALGEPA SYAEYRRRLE KSKQD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtcgcg ggcaaatcat tggtgttgtg ctgcgagaag ccctaccaaa gtacgcggca tccatggcgt cggagctgct cgcatccgct gccgtgcgca cctcgcttcg gagcatgatg tttggcggag agtcgccctt gagcggtatt gctggctact tcgactaccg gggctcaccg gtggagctga agtcacgcaa aacgtccttc acatatgaac acgaggcagc gtggccggcg gtcttccctg tcgtcgatta cgtcagcgag ctctacaggc atgtcgcgcc ggagcggtgg aaagcacaaa acgacgccat tccggatgtt gtgcgtattc acggaacccc gttctccacg cttaccatca actcccgctt ccgcacagcc tctcataccg acgtcggcga ttttgacggt ggctacagct gcattgcttg ccttgacggg cagttcaaag ggctggccct tgctttcgac gacttcggca ttaacgtgct catgcagccg cgcgatgtga tgatattcga ctcgcaccac ttccactcaa acacggaggt ggagctgtcg ttctcagggg aggactggaa gcgacttacc tgcgtattct actaccgggc cgccctcggt gagccagcaa gctacgccga gtaccggcgc cggttggaga agtccaagca ggactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat