LeinA.19529.a

J2IP1

CENTER ID: LeinA.19529.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.19529.a.B2.GE41471 ordered domain 13 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.03.0020
UniProt: E9AG22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTGAADKKH LVPLLEFLRF VSERGRSQAA RFPRRINGVT DMKCEPCDSS IEADSLPVKH EFNGEVAYSS SYDSSPSASR ENAVPMLPAE EWRKRSRSDD EISEVIVGSS APIDCELEFM TTGKGCVTSK YFDFSLLDLG AVPMIEPAVE VLTKDVAKQI QGKGCCYVLP GTSMVVTGDI VDVSLASETV LVSPDRGLRV GLEWVSVRRV FHVPHNIAAV REEAAFRIQS IIEAERRGER TPRFSPVQLA ETAEELSSPL SPDSQEDA
NT Sequence
atgaccaccg gtgcagccga caagaagcac ctagtaccac ttctggagtt tttacgattt gtatcggaac gaggcaggag ccaagcagcc cgatttccga ggcggatcaa cggtgtcacc gacatgaagt gcgaaccgtg cgattccagt atagaggcag actcgcttcc agtgaagcac gaatttaacg gcgaggttgc ctacagcagc agctatgata gctctccttc cgcgtcgcgc gagaacgctg ttcctatgtt accagctgaa gagtggcgaa agcggagtcg ctctgatgat gaaatttcgg aggtgattgt tggctcaagt gcgcccatcg actgcgaact tgagttcatg acgactggaa agggatgcgt cacgtcaaaa tactttgatt tttcattgct tgatttaggt gcggtgccga tgatcgaacc agccgtggaa gtccttacga aagatgttgc aaagcaaata cagggcaaag gatgctgcta cgtgcttcct ggtacttcca tggttgtgac aggtgacatt gtggatgtca gcctcgcgtc cgaaacggtt ctcgtctctc ctgaccgtgg attacgagtc ggcttggaat gggtgtcggt tcggcgcgtg ttccacgtgc cgcacaacat agcggccgtg cgggaagaag cggcgttccg aattcagagt atcatcgaag cagagcggcg aggggaaaga acaccaaggt tttcgcctgt ccagctggcg gagactgcag aggagctgtc atcacctttg tcacctgaca gccaagagga cgcgtag
Details for LeinA.19529.a.B2.GE41471
HARVESTED ON: 4/28/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 28kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHHH MPLLEFLRFV SERGRSQAAR FPRRINGVTD MKCEPCDSSI EADSLPVKHE FNGEVAYSSS YDRSPSASRE NAVPMLPAEG WRKRSRSDDE ISEVIVGSSA PIDCELEFMT TGKGCVTSKY FDFSLLDLGA VPMIEPAVEV LTKDVAKQIQ GKGCCYVLPG TSMVVTGDIV DVSLASETVL VSPDRGLRVG LEWVSVRRVF HVPHNIAAVR EEAAFRIQSI IEAERRGERT PRFSPVLLAE TAEEL
Validated NT Sequence
atggctcacc accaccacca ccaccaccat atgccacttc tggagttttt acgatttgta tcggaacgag gcaggagcca agcagcccga tttccgaggc ggatcaacgg tgtcaccgac atgaagtgcg aaccgtgcga ttccagtata gaggcagact cgcttccagt gaagcatgaa tttaacggcg aggttgccta cagcagcagc tatgataggt ctccttccgc gtcgcgcgag aacgctgttc ctatgttacc agctgaaggg tggcgaaagc ggagtcgctc tgatgatgaa atttcggagg tgattgttgg ctcaagtgcg cccatcgact gcgaacttga gttcatgacg actggaaagg gatgcgtcac gtcaaaatac tttgattttt cattgcttga tttaggtgcg gtgccgatga tcgaaccagc cgtggaagtc cttacgaaag atgttgcaaa gcaaatacag ggcaaaggat gctgctacgt gcttcctggt acttccatgg ttgtgacagg tgacattgtg gatgtcagcc tcgcgtccga aacggttctc gtctctcctg accgtggatt acgagtcggc ttggaatggg tgtcggttcg gcgcgtgttc cacgtgccgc acaacatagc ggccgtgcgg gaagaagcgg cgttccgaat tcagagtatc atcgaagcag agcggcgagg ggaaagaaca ccaaggtttt cgcctgtcct gctggcggag actgcagagg agctgtaagt gagtaagat
Expected Protein Sequence
MAHHHHHHPL LEFLRFVSER GRSQAARFPR RINGVTDMKC EPCDSSIEAD SLPVKHEFNG EVAYSSSYDS SPSASRENAV PMLPAEEWRK RSRSDDEISE VIVGSSAPID CELEFMTTGK GCVTSKYFDF SLLDLGAVPM IEPAVEVLTK DVAKQIQGKG CCYVLPGTSM VVTGDIVDVS LASETVLVSP DRGLRVGLEW VSVRRVFHVP HNIAAVREEA AFRIQSIIEA ERRGERTPRF SPVQLAETAE EL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccacttc tggagttttt acgatttgta tcggaacgag gcaggagcca agcagcccga tttccgaggc ggatcaacgg tgtcaccgac atgaagtgcg aaccgtgcga ttccagtata gaggcagact cgcttccagt gaagcacgaa tttaacggcg aggttgccta cagcagcagc tatgatagct ctccttccgc gtcgcgcgag aacgctgttc ctatgttacc agctgaagag tggcgaaagc ggagtcgctc tgatgatgaa atttcggagg tgattgttgg ctcaagtgcg cccatcgact gcgaacttga gttcatgacg actggaaagg gatgcgtcac gtcaaaatac tttgattttt cattgcttga tttaggtgcg gtgccgatga tcgaaccagc cgtggaagtc cttacgaaag atgttgcaaa gcaaatacag ggcaaaggat gctgctacgt gcttcctggt acttccatgg ttgtgacagg tgacattgtg gatgtcagcc tcgcgtccga aacggttctc gtctctcctg accgtggatt acgagtcggc ttggaatggg tgtcggttcg gcgcgtgttc cacgtgccgc acaacatagc ggccgtgcgg gaagaagcgg cgttccgaat tcagagtatc atcgaagcag agcggcgagg ggaaagaaca ccaaggtttt cgcctgtcca gctggcggag actgcagagg agctgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat