LeinA.19528.a

J-binding protein 2 (JBP2)

CENTER ID: LeinA.19528.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LeinA.19528.a.B1.GE41466 full length 1 1098
LeinA.19528.a.B2.GE41467 N-term conserved domain 14 162
LeinA.19528.a.B3.GE41468 Tet_JBP oxygenase 240 512
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79433
de-novo with evolutionary constraints ModelArchive_ma-xp9dq
de-novo with evolutionary constraints ModelArchive_ma-uipso
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.14.0040
UniProt: A4HVU6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNGLTRVST SSELESILDI VQSSGEIAVV FTSPSIGDLE TITSETQRRQ LRIAGIPRGG YTILPAIPLY DDELLQMCER YTSANEYEKA EMRNSLYMRE YPLFTYSIRH QRALFHPADY VSRILQFCSY YVQAPDADVL PLQDKSPFLH ISPIKEICKH LRLIARGTPV APDDSESPVP EQLRLHAESD AEKLAAERAT AMSIATSSGG ASETEQPSLF SGVAPSALFQ KGAVEEVDKD AEDTMEDLTG EETVDAVHSF QAEYLTLDGF ELVTKASIYY DREGEGQRVV AVYIPGGVPE DTCRAAAAVL EPAATKKNLR APTNGGLPPD TGIVGYYDYL TNPTQHKCRE TEYSRRNWGL LAQSEPLLKH LDKLYSQLAP MHHHLQRVAI PSQYQLCGTV FSTITVNRNF RTAVHTDKGD FRSGLGVLSV INGEFEGCHL AIKRLKKAFQ LKVGDVLLFD TSLEHGNTEV VNPEIHWQRT SVVCYLRTGL MSSVCEMERR KHLNRLILLQ LLNTEVRNTT VNINGADSSL PPLFVPTRLA SHLAPVQLAA LGFIVERTEK QSGCVVAMTM GLGKTLVALT LCFSQLHLAP QADILILTPK PIISHWVDEK NKWGMHGLHF PHFVASDGLN SLEFEQQLLE YERQRNNEKP KLGHIFVING EYLAGFLRRF KRFTPLLMIV DEGHRVAAKG NKLTESLDRL RCNLRIVLSG TPLQNDASEL YRLVGWVNKG VSRVLPPKRF QELANDINQF VEGDDGAFYN AVMAQEYIQD WMRGFVFREM ENDLPPLHDY LLICGSSDVQ REYEEKLGLT ETAMTALKAT EHRPHHLSTH PACYLAFISD SYQSMVSGWT VRAQSNTSRP RVSQLEEIDT MRLEQYVQLV ENEQLDAFID LSGKMRVLVD IVLRVQARKE KLIIFSLYVG SQDLIHRTLT ALRVCTFTVR GRDSQDRRRR AMQEFSENKD LIVLVLSTKI AAYGLDFTAA NHVVLFDSWW NPQVDAQAIA RAYRRNQRKP VTVYRLISAT ENKFVLRSQT RKIALFKCIL HERTSRQALP DELEDCAANE KDEERRIFWA KLKTTSLAGD SRALLNVYRY QESVRESE
NT Sequence
atgttaaatg ggctgacgcg ggtgagcacc agttctgagc tggagtcgat actagatatc gtgcaatcgt ccggcgaaat cgcggtcgta ttcacttcgc cgagcattgg cgatttggag acgattacaa gcgaaacaca gcgtcgtcag ctgcgcattg cgggaatccc tcgcggtggg tacaccatcc tccctgctat tccgctatac gatgacgagc tgcttcagat gtgtgaacgg tacacctcag cgaacgagta cgagaaggcg gaaatgcgca acagcttgta tatgcgagaa tacccactat tcacgtacag catacgccat cagagggcac tatttcaccc tgccgactac gtgagccgga tactccagtt ctgctcctat tatgtccagg cgcccgacgc agacgttttg ccgctgcaag acaagagtcc ttttcttcac atttccccca taaaggaaat ttgcaagcat ctccgcctca ttgcccgcgg caccccagtg gcgccagacg actccgagtc gccggtgcca gagcagctgc gccttcatgc agagtcagac gcggagaagc ttgcagccga gcgtgccacg gctatgtcca tcgcaaccag tagtggtggc gcctcggaga cggagcagcc gtctctcttc agcggcgttg cgccgtcggc gctgtttcag aaaggcgcag tggaggaggt cgacaaggac gctgaagaca ccatggagga tctcaccggc gaggagacag tggacgcggt gcacagcttt caggcagagt acttgacact ggacgggttt gagctggtga caaaagcgtc catctactac gaccgcgaag gggaggggca gcgcgtcgtt gcggtgtaca ttccgggagg ggtaccagag gacacgtgcc gcgcagccgc agcggtcctg gagccggcgg cgacaaaaaa gaatctgcgg gcacccacga acggtggact gccaccagac accgggatcg taggctacta cgactacctt acgaatccta cccaacacaa gtgcagagag acggaataca gccgcaggaa ttgggggctc ttagcccaga gcgagccgtt gctgaagcat ctcgacaagc tgtacagtca gttggctcca atgcaccatc atctgcagag ggtggccatc ccgtcacagt accagctgtg cggaacagtc ttcagcacca tcactgttaa tagaaacttc cgcaccgccg tgcacaccga caagggtgac ttccggagcg gcttgggggt tctctcagtg atcaacggcg agtttgaggg atgccatctc gccatcaaaa ggctcaagaa ggcgttccag ctcaaagtcg gcgacgttct tcttttcgac acgtctctgg agcacggcaa cacggaggtg gtaaacccgg aaattcactg gcagcgaacc agcgttgtgt gctacctgcg cactggacta atgtcctctg tgtgcgagat ggagcgacgt aagcacctga atcggctcat tctcctgcaa ctgctcaaca cagaggttcg caacacgacg gtgaacatca acggagccga cagcagcctg ccaccgctgt ttgtgccgac ccgcctggcg agccatttgg cgcccgtgca gcttgctgcc ctcggcttca ttgtggagcg tacggagaag cagagtgggt gcgtggtggc aatgacgatg ggactgggca aaacgcttgt tgcactcacg ttgtgcttct cacagctgca tcttgcaccg caggccgaca tactcatcct cacgccaaaa ccgatcatca gtcactgggt ggacgagaaa aacaagtggg gcatgcacgg gctccatttt ccgcacttcg tcgcctccga cgggctcaac tcgttggagt tcgagcagca gcttctcgag tacgagcgtc agaggaacaa cgagaaaccc aagttgggtc acatcttcgt catcaacggc gagtacctgg cagggtttct gcgacgtttc aagcgcttta cgcccttgct catgatcgtg gacgagggcc accgagtggc tgcgaagggg aataaattga cggagtccct cgatcgccta cggtgcaacc tccgcatcgt gctatccggc acgcctttgc aaaacgacgc cagcgaactc taccggctag tggggtgggt gaacaaaggc gtcagcaggg tactgccgcc gaagcgcttc caggagctcg cgaatgacat caaccagttc gtcgaaggcg acgatggggc cttctacaat gcagtgatgg cgcaggagta cattcaggac tggatgcgtg ggttcgtgtt tcgtgaaatg gaaaacgacc tgccaccgct gcacgattac ctgttgattt gcggctcctc cgacgtgcag cgggagtatg aggagaagct gggcctcacg gagacggcca tgacagccct taaagccacg gagcaccgac cgcatcacct ctccacgcac cccgcttgct acctggcctt catctccgac agctaccaat cgatggtaag cgggtggaca gtgcgtgcgc agtcgaatac gtcgcggccg cgagtgtccc aactggagga gattgatacc atgcgactgg agcagtatgt ccagttggtc gagaacgagc aactcgacgc cttcatcgac ttgagcggca agatgcgggt gctcgtcgat attgtgctgc gcgtgcaagc ccgcaaggag aagctcatca ttttctctct ctacgtgggt tcgcaggacc taattcatcg caccctgacg gcgctgcggg tctgcacatt taccgttcgc ggccgagact cacaagaccg ccgccgccgc gccatgcagg agttcagcga gaacaaagac ctcattgtgc tggtgttatc aacaaagatc gccgcctacg gtctcgactt cacggcggcg aatcacgttg ttctgttcga ctcgtggtgg aacccgcagg tggacgcaca agccatcgcg cgcgcgtaca ggcgaaatca acggaagcca gtgaccgtgt atcgccttat ctcggcaacc gaaaacaagt tcgtgctgcg ctcacagaca cgaaagattg ccctcttcaa gtgcatcctc catgagcgca ccagtcggca ggcgttgccc gacgagctgg aggattgcgc agcgaacgag aaggatgaag aaaggcgaat cttttgggca aaactgaaaa cgacctctct tgctggagat tctcgggcgt tgttgaacgt ttatcgctac caggagagcg ttcgcgaaag cgaatga
Details for LeinA.19528.a.B1.GE41466
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 125kDa
OBSERVED MW: 125kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML NGLTRVSTSS ELESILDIVQ SSGEIAVVFT SPSIGDLETI TSETQRRQLR IAGIPRGGYT ILPAIPLYDD ELLQMCERYT SANEYEKAEM RNSLYMREYP LFTYSIRHQR ALFHPADYVS RILQFCSYYV QAPDADVLPL QDKSPFLHIS PIKEICKHLR LIARGTPVAP DDSESPVPEQ LRLHAESDAE KLAAERATAM SIATSSGGAS ETEQPSLFSG VAPSALFQKG AVEEVDKDAE DTMEDLTGEE TVDAVHSFQA EYLTLDGFEL VTKASIYYDR EGEGQRVVAV YIPGGVPEDT CRAAAAVLEP AATKKNLRAP TNGGLPPDTG IVGYYDYLTN PTQHKCRETE YSRRNWGLLA QSEPLLKHLD KLYSQLAPMH HHLQRVAIPS QYQLCGTVFS TITVNRNFRT AVHTDKGDFR SGLGVLSVIN GEFEGCHLAI KRLKKAFQLK VGDVLLFDTS LEHGNTEVVN PEIHWQRTSV VCYLRTGLMS SVCEMERRKH LNRLILLQLL NTEVRNTTVN INGADSSLPP LFVPTRLASH LAPVQLAALG FIVERTEKQS GCVVAMTMGL GKTLVALTLC FSQLHLAPQA DILILTPKPI ISHWVDEKNK WGMHGLHFPH FVASDGLNSL EFEQQLLEYE RQRNNEKPKL GHIFVINGEY LAGFLRRFKR FTPLLMIVDE GHRVAAKGNK LTESLDRLRC NLRIVLSGTP LQNDASELYR LVGWVNKGVS RVLPPKRFQE LANDINQFVE GDDGAFYNAV MAQEYIQDWM RGFVFREMEN DLPPLHDYLL ICGSSDVQRE YEEKLGLTET AMTALKATEH RPHHLSTHPA CYLAFISDSY QSMVSGWTVR AQSNTSRPRV SQLEEIDTMR LEQYVQLVEN EQLDAFIDLS GKMRVLVDIV LRVQARKEKL IIFSLYVGSQ DLIHRTLTAL RVCTFTVRGR DSQDRRRRAM QEFSENKDLI VLVLSTKIAA YGLDFTAANH VVLFDSWWNP QVDAQAIARA YRRNQRKPVT VYRLISATEN KFVLRSQTRK IALFKCILHE RTSRQALPDE LEDCAANEKD EERRIFWAKL KTTSLAGDSR ALLNVYRYQE SVRESE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa atgggctgac gcgggtgagc accagttctg agctggagtc gatactagat atcgtgcaat cgtccggcga aatcgcggtc gtattcactt cgccgagcat tggcgatttg gagacgatta caagcgaaac acagcgtcgt cagctgcgca ttgcgggaat ccctcgcggt gggtacacca tcctccctgc tattccgcta tacgatgacg agctgcttca gatgtgtgaa cggtacacct cagcgaacga gtacgagaag gcggaaatgc gcaacagctt gtatatgcga gaatacccac tattcacgta cagcatacgc catcagaggg cactatttca ccctgccgac tacgtgagcc ggatactcca gttctgctcc tattatgtcc aggcgcccga cgcagacgtt ttgccgctgc aagacaagag tccttttctt cacatttccc ccataaagga aatttgcaag catctccgcc tcattgcccg cggcacccca gtggcgccag acgactccga gtcgccggtg ccagagcagc tgcgccttca tgcagagtca gacgcggaga agcttgcagc cgagcgtgcc acggctatgt ccatcgcaac cagtagtggt ggcgcctcgg agacggagca gccgtctctc ttcagcggcg ttgcgccgtc ggcgctgttt cagaaaggcg cagtggagga ggtcgacaag gacgctgaag acaccatgga ggatctcacc ggcgaggaga cagtggacgc ggtgcacagc tttcaggcag agtacttgac actggacggg tttgagctgg tgacaaaagc gtccatctac tacgaccgcg aaggggaggg gcagcgcgtc gttgcggtgt acattccggg aggggtacca gaggacacgt gccgcgcagc cgcagcggtc ctggagccgg cggcgacaaa aaagaatctg cgggcaccca cgaacggtgg actgccacca gacaccggga tcgtaggcta ctacgactac cttacgaatc ctacccaaca caagtgcaga gagacggaat acagccgcag gaattggggg ctcttagccc agagcgagcc gttgctgaag catctcgaca agctgtacag tcagttggct ccaatgcacc atcatctgca gagggtggcc atcccgtcac agtaccagct gtgcggaaca gtcttcagca ccatcactgt taatagaaac ttccgcaccg ccgtgcacac cgacaagggt gacttccgga gcggcttggg ggttctctca gtgatcaacg gcgagtttga gggatgccat ctcgccatca aaaggctcaa gaaggcgttc cagctcaaag tcggcgacgt tcttcttttc gacacgtctc tggagcacgg caacacggag gtggtaaacc cggaaattca ctggcagcga accagcgttg tgtgctacct gcgcactgga ctaatgtcct ctgtgtgcga gatggagcga cgtaagcacc tgaatcggct cattctcctg caactgctca acacagaggt tcgcaacacg acggtgaaca tcaacggagc cgacagcagc ctgccaccgc tgtttgtgcc gacccgcctg gcgagccatt tggcgcccgt gcagcttgct gccctcggct tcattgtgga gcgtacggag aagcagagtg ggtgcgtggt ggcaatgacg atgggactgg gcaaaacgct tgttgcactc acgttgtgct tctcacagct gcatcttgca ccgcaggccg acatactcat cctcacgcca aaaccgatca tcagtcactg ggtggacgag aaaaacaagt ggggcatgca cgggctccat tttccgcact tcgtcgcctc cgacgggctc aactcgttgg agttcgagca gcagcttctc gagtacgagc gtcagaggaa caacgagaaa cccaagttgg gtcacatctt cgtcatcaac ggcgagtacc tggcagggtt tctgcgacgt ttcaagcgct ttacgccctt gctcatgatc gtggacgagg gccaccgagt ggctgcgaag gggaataaat tgacggagtc cctcgatcgc ctacggtgca acctccgcat cgtgctatcc ggcacgcctt tgcaaaacga cgccagcgaa ctctaccggc tagtggggtg ggtgaacaaa ggcgtcagca gggtactgcc gccgaagcgc ttccaggagc tcgcgaatga catcaaccag ttcgtcgaag gcgacgatgg ggccttctac aatgcagtga tggcgcagga gtacattcag gactggatgc gtgggttcgt gtttcgtgaa atggaaaacg acctgccacc gctgcacgat tacctgttga tttgcggctc ctccgacgtg cagcgggagt atgaggagaa gctgggcctc acggagacgg ccatgacagc ccttaaagcc acggagcacc gaccgcatca cctctccacg caccccgctt gctacctggc cttcatctcc gacagctacc aatcgatggt aagcgggtgg acagtgcgtg cgcagtcgaa tacgtcgcgg ccgcgagtgt cccaactgga ggagattgat accatgcgac tggagcagta tgtccagttg gtcgagaacg agcaactcga cgccttcatc gacttgagcg gcaagatgcg ggtgctcgtc gatattgtgc tgcgcgtgca agcccgcaag gagaagctca tcattttctc tctctacgtg ggttcgcagg acctaattca tcgcaccctg acggcgctgc gggtctgcac atttaccgtt cgcggccgag actcacaaga ccgccgccgc cgcgccatgc aggagttcag cgagaacaaa gacctcattg tgctggtgtt atcaacaaag atcgccgcct acggtctcga cttcacggcg gcgaatcacg ttgttctgtt cgactcgtgg tggaacccgc aggtggacgc acaagccatc gcgcgcgcgt acaggcgaaa tcaacggaag ccagtgaccg tgtatcgcct tatctcggca accgaaaaca agttcgtgct gcgctcacag acacgaaaga ttgccctctt caagtgcatc ctccatgagc gcaccagtcg gcaggcgttg cccgacgagc tggaggattg cgcagcgaac gagaaggatg aagaaaggcg aatcttttgg gcaaaactga aaacgacctc tcttgctgga gattctcggg cgttgttgaa cgtttatcgc taccaggaga gcgttcgcga aagcgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LeinA.19528.a.B2.GE41467
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML ESILDIVQSS GEIAVVFTSP SIGDLETITS ETQRRQLRIA GIPRGGYTIL PAIPLYDDEL LQMCERYTSA NEYEKAEMRN SLYMREYPLF TYSIRHQRAL FHPADYVSRI LQFCSYYVQA PDADVLPLQD KSPFLHISPI KEICKHLR
Validated NT Sequence
atggctcacc accaccacca ccatatgctg gagtcgatac tagatatcgt gcaatcgtcc ggcgaaatcg cggtcgtatt cacttcgccg agcattggcg atttggagac gattacaagc gaaacacagc gtcgtcagct gcgcattgcg ggaatccctc gcggtgggta caccatcctc cctgctattc cgctatacga tgacgagctg cttcagatgt gtgaacggta cacctcagcg aacgagtacg agaaggcgga aatgcgcaac agcttgtata tgcgagaata cccactattc acgtacagca tacgccatca gagggcacta tttcaccctg ccgactacgt gagccggata ctccagttct gctcctatta tgtccaggcg cccgacgcag acgttttgcc gctgcaagac aagagtcctt ttcttcacat ttcccccata aaggaaattt gcaagcatct ccgctaagtg agtaagat
Expected Protein Sequence
MAHHHHHHLE SILDIVQSSG EIAVVFTSPS IGDLETITSE TQRRQLRIAG IPRGGYTILP AIPLYDDELL QMCERYTSAN EYEKAEMRNS LYMREYPLFT YSIRHQRALF HPADYVSRIL QFCSYYVQAP DADVLPLQDK SPFLHISPIK EICKHLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctggagt cgatactaga tatcgtgcaa tcgtccggcg aaatcgcggt cgtattcact tcgccgagca ttggcgattt ggagacgatt acaagcgaaa cacagcgtcg tcagctgcgc attgcgggaa tccctcgcgg tgggtacacc atcctccctg ctattccgct atacgatgac gagctgcttc agatgtgtga acggtacacc tcagcgaacg agtacgagaa ggcggaaatg cgcaacagct tgtatatgcg agaataccca ctattcacgt acagcatacg ccatcagagg gcactatttc accctgccga ctacgtgagc cggatactcc agttctgctc ctattatgtc caggcgcccg acgcagacgt tttgccgctg caagacaaga gtccttttct tcacatttcc cccataaagg aaatttgcaa gcatctccgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LeinA.19528.a.B3.GE41468
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 32kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD AEDTMEDLTG EETVDAVHSF QAEYLTLDGF ELVTKASIYY DREGEGQRIV AVYIPGGVPE DTCRAAAAVL EPAATKKNLR APTNGGLPPD TGIVGYYDYL TNPTQHKCRE TEYSRRNWGL LAQSEPLLKH LDKLYSQLAP MHHHLQRVAI PSQYQLCGTV FSTITVNRNF RTAVHTDKGD FRSGLGVLSV INGEFEGCHL AIKRLKKAFQ LKVGDVLLFD TSLEHGNTEV VNPEIHWQRT SVVCYLRTGL MSSVCEMERR KHLNRLILLQ LL
Validated NT Sequence
atggctcacc accaccacca ccatatggac gctgaagaca ccatggagga tctcaccggc gaggagacag tggacgcggt gcacagcttt caggcagagt acttgacact ggacgggttt gagctggtga caaaagcgtc catctactac gaccgcgaag gggaggggca gcgcatcgtt gcggtgtaca ttccgggagg ggtaccagag gacacgtgcc gcgcagccgc agcggtcctg gagccggcgg cgacaaaaaa gaatctgcgg gcacccacga acggtggact gccaccagac accgggatcg taggctacta cgactacctt acgaatccta cccaacacaa gtgcagagag acggaataca gccgcaggaa ttgggggctc ttagcccaga gcgagccgtt gctgaagcat ctcgacaagc tgtacagtca gttggctcca atgcaccatc atctgcagag ggtggccatc ccgtcacagt accagctgtg cggaacagtc ttcagcacca tcactgttaa tagaaacttc cgcaccgccg tgcacaccga caagggtgac ttccggagcg gcttgggggt tctctcagtg atcaacggcg agtttgaggg atgccatctc gccatcaaaa ggctcaagaa ggcgttccag ctcaaagtcg gcgacgttct tcttttcgac acgtctctgg agcacggcaa cacggaggtg gtaaacccgg aaattcactg gcagcgaacc agcgttgtgt gctacctgcg cactggacta atgtcctctg tgtgcgagat ggagcgacgt aagcacctga atcggctcat tctcctgcaa ctgctctaag tgagtaagat
Expected Protein Sequence
MAHHHHHHDA EDTMEDLTGE ETVDAVHSFQ AEYLTLDGFE LVTKASIYYD REGEGQRVVA VYIPGGVPED TCRAAAAVLE PAATKKNLRA PTNGGLPPDT GIVGYYDYLT NPTQHKCRET EYSRRNWGLL AQSEPLLKHL DKLYSQLAPM HHHLQRVAIP SQYQLCGTVF STITVNRNFR TAVHTDKGDF RSGLGVLSVI NGEFEGCHLA IKRLKKAFQL KVGDVLLFDT SLEHGNTEVV NPEIHWQRTS VVCYLRTGLM SSVCEMERRK HLNRLILLQL L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgctg aagacaccat ggaggatctc accggcgagg agacagtgga cgcggtgcac agctttcagg cagagtactt gacactggac gggtttgagc tggtgacaaa agcgtccatc tactacgacc gcgaagggga ggggcagcgc gtcgttgcgg tgtacattcc gggaggggta ccagaggaca cgtgccgcgc agccgcagcg gtcctggagc cggcggcgac aaaaaagaat ctgcgggcac ccacgaacgg tggactgcca ccagacaccg ggatcgtagg ctactacgac taccttacga atcctaccca acacaagtgc agagagacgg aatacagccg caggaattgg gggctcttag cccagagcga gccgttgctg aagcatctcg acaagctgta cagtcagttg gctccaatgc accatcatct gcagagggtg gccatcccgt cacagtacca gctgtgcgga acagtcttca gcaccatcac tgttaataga aacttccgca ccgccgtgca caccgacaag ggtgacttcc ggagcggctt gggggttctc tcagtgatca acggcgagtt tgagggatgc catctcgcca tcaaaaggct caagaaggcg ttccagctca aagtcggcga cgttcttctt ttcgacacgt ctctggagca cggcaacacg gaggtggtaa acccggaaat tcactggcag cgaaccagcg ttgtgtgcta cctgcgcact ggactaatgt cctctgtgtg cgagatggag cgacgtaagc acctgaatcg gctcattctc ctgcaactgc tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat