SSGCID: Target Page

LeinA.19527.a

Glucosyl transferase (HmdUGT)

CENTER ID:	LeinA.19527.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
LeinA.19527.a.B1.GE41463	full length	1	954
LeinA.19527.a.B2.GE41464	nucleoside diphospho-sugar transferase-like domain	22	740
LeinA.19527.a.B3.GE41465	C-term domain	814	954

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.19527.a.B2.PS38314	nucleoside diphospho-sugar transferase-like domain		22	740

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79412

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LinJ.36.2500
UniProt:	A4IDL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFSLNIKTAA SPSSAAPAPA PEVNAGEAPW AIIDVDEADS FESTPSTNSS GGQGGRHRYQ HAAQPSHSPQ SPETPIKFDH GAYPHCPIFI PSKGRSDLDR STMAVLVRDL VPFVLVVERE EADAYRAMLD RLVGQYFGFA GMTFAANVDD GIEGATKTGA EVVVSSSTTP ADLWAFPCAC CCGSTDVAGS PSSPTTPGLS EDALRALALR LSAAFHHGYV FEPSASAAAG DHHGAVESNV SPPVSISPTA PSAASGSARR PSAAFVLRNV DEVRALFVIE VLPETNRGVS YVRNYILQVL VPRLMVACGV DRSGNVEELR LCGTGEHNTL TSAALRMAAL EPDVYSALEW KSETESYLDA RRASTTTNVV TTSTGSAAGT RDGVVSASVG ALSAPTGQIL APAFSSSSAP RSSKPSSAAA ATRGTPRLLH GLFGFYWVLD DDIYGFYQSH GSSTKNERIS TRAMMREVDM RLRQLRHNAD VASPPPPSVP PSCFQTPPTH SSGSAAASPY VLSHPQQQQN FVFTCKEDAL QNNFISGGKG RLPTLQNYNL YYTTACFSLE YNRFALDTAP DTLSVNSYNN IACLFNYALL HNPPQMRYFP PGVPHVRRAE DALVGYLDHS MLWYRFAVRE DYDFTLQLIA RGLHTLRFRS IAFDVPQMMK VRGGMTDYYR NCHEEIRQQN DRFVLQWPAV AQHWVKGKEG NRRDDIRVRW DLLSPARAKH PGAFLYLSTP LPQLQPQRLS DDSTGGNGGV VSSSDSAATA TAVEGAETET RKRLRPPSPT APTAESSLAP AGRRPCVQAA ATSAPAMSST AARDWKGGYV VERWRDISRE EARQHAGLVA LSNDDIHIGR TVAVIPPFFD QKPSVVRATV IERAVEPTDS HSSSGRSGNA SAAEAGDAQQ SLTPRVIWTA VAQDVRGMPF LHVDTCFEVP AEGIEKAAAS IDQFFAKMEG EPGP

NT Sequence

atgttctccc tcaacatcaa aacagcagcc tcgccgtcct ctgcagctcc cgcaccggca ccggaagtga acgccggcga ggcgccatgg gcgatcattg acgtggacga agccgactcc tttgaaagca caccctcgac gaacagcagc ggtggtcagg gcggccgtca tcgttatcag catgccgcgc agccgtcgca ctctccgcag agtcccgaga cgcccatcaa atttgaccac ggtgcatacc cgcactgccc catcttcatc ccctccaaag gtcgctcaga cctggaccgc agcaccatgg cggtgctcgt ccgcgatctc gtcccgtttg tgcttgtcgt cgagcgtgag gaggccgatg cgtacagagc tatgctggac cgcctcgttg gacaatactt cggcttcgcc ggtatgactt ttgccgcgaa cgtggacgat ggcattgaag gggcaaccaa gacgggggca gaggtcgtcg tgtcgtccag cactactccg gcagacctct gggcgttccc gtgcgcctgc tgctgcggca gcactgacgt cgctggttcg ccgtcgtcgc ccaccacacc cggcctatcg gaggatgcct tgcgcgcgtt ggcgctgcgg ctttcagctg cgtttcatca tggctatgtt ttcgagccgt cagcgtctgc tgcagccggc gaccaccacg gcgctgttga gtcgaatgtc tcaccgcctg tgtcgatcag tccaacagcg ccatcagcag ccagcgggag tgcgcggcgc ccctccgcgg cctttgtgct gcgcaacgtg gacgaggtcc gcgcgctctt cgtgatcgaa gtgctgccgg agaccaatcg tggggtctcc tatgtgcgca actacatcct acaggttctc gtgccaaggc tgatggtggc ctgcggcgta gatcggtcag ggaacgtgga ggagctgcgc ctgtgtggca caggagagca caacacgctg acatcggcgg cgctgcggat ggccgcgctg gagccagacg tctacagtgc ccttgagtgg aagtcagaga ccgagtccta tctggacgca cggcgggcga gcaccaccac gaacgtggta acgacaagca ctggcagcgc cgcgggtacc agagatggcg tcgtgtcggc gtcggtggga gcgttgagcg ccccaaccgg acaaattttg gcgccagcgt tctcgtcgtc gtctgcgcca cgctcgtcaa agccgtcctc cgctgcggcc gcgactcgcg gcacgcctcg ccttctgcac ggcctcttcg gcttctactg ggtcctggat gacgacatct acggctttta ccagtcgcac ggctccagca ccaaaaacga gcgcattagt acgcgggcga tgatgagaga ggtggacatg cggctgcggc aactgcggca caacgccgac gtggcctcgc caccaccgcc gtcagttccg ccttcgtgct ttcagacacc gccgacccac agcagcggca gcgctgccgc atcgccatat gtcttgtcac acccgcagca acagcaaaat ttcgtgttca cgtgcaagga ggatgcccta cagaacaatt tcatctctgg agggaaaggc cggcttccga ccctgcagaa ctacaacctc tactacacaa ccgcctgctt ctcgctcgag tacaaccgct tcgccctcga cacggctccg gacacgctgt cggtcaactc ctacaataac attgcctgtc tcttcaacta cgcgctgctg cacaacccgc cgcagatgcg ctacttcccc cctggggtgc cgcacgtgag gcgcgccgag gacgcgctgg tcggctacct cgatcacagc atgctctggt accgcttcgc cgtgcgcgaa gattacgact tcactcttca gctcatcgca cgtggcctcc acacgctgcg cttccgcagc atcgccttcg atgtgcctca gatgatgaag gtgcgtggcg gcatgacgga ctactatcgc aactgccacg aagagatacg acagcagaac gatcgctttg tgctgcagtg gccggccgtg gcgcagcact gggtgaaggg gaaggagggc aaccgtcgag acgacatacg tgtgcggtgg gacctgctga gcccagcccg cgccaagcac cccggtgcct tcctgtatct gagcacccca ctgccgcagc tgcagccgca acgcttgagt gacgacagca ccggcggaaa cggcggtgta gttagcagca gcgacagtgc agccactgca accgcggtgg aaggcgcgga aacagagacg cgaaagcgtc tgcggccccc gtccccgact gctcccactg ctgaatcgtc acttgcccct gcaggccgtc gcccctgcgt ccaggccgca gcaacgtctg cgcccgctat gtcgtccacc gctgcccgtg actggaaagg tggctacgtg gtcgagcggt ggcgtgatat ctcacgtgag gaggcgcggc agcacgccgg cctcgtcgcc ctctccaacg atgacatcca cattggccgg actgtcgctg tcataccgcc cttcttcgac cagaaaccat ctgtcgtgcg cgcgacagtg attgagcggg ccgtcgagcc aacggactcc cattccagca gcggccgctc tgggaacgcc agcgcggcgg aagcaggtga tgcccagcag tccctaacgc cgcgcgtgat ctggacagcg gtggcacaag atgttcgtgg tatgcctttc ttgcacgttg acacatgctt tgaggtgccc gcggagggca tcgaaaaggc cgccgccagc atcgatcagt tcttcgcaaa gatggagggc gagcctggcc catag

Details for LeinA.19527.a.B1.GE41463

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	104kDa
OBSERVED MW:	104kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMF SLNIKTAASP SSAAPAPAPE VNAGEAPWAI IDVDEADSFE STPSTNSSGG QGGRHRYQHA AQPSHSPQSP ETPIKFDHGA YPHCPIFIPS KGRSDLDRST MAVLVRDLVP FVLVVEREEA DAYRAMLDRL VGQYFGFAGM TFAANVDDGI EGATKTGAEV VVSSSTTPAD LWAFPCACCC GSTDVAGSPS SPTTPGLSED ALRALALRLS AAFHHGYVFE PSASAAAGDH HGAVESNVSP PVSISPTAPS AASGSARRPS AAFVLRNVDE VRALFVIEVL PETNRGVSYV RNYILQVLVP RLMVACGVDR SGNVEELRLC GTGEHNTLTS AALRMAALEP DVYSALEWKS ETESYLDARR ASTTTNVVTT STGSAAGTRD GVVSASVGAL SAPTGQILAP AFSSSSAPRS SKPSSAAAAT RGTPRLLHGL FGFYWVLDDD IYGFYQSHGS STKNERISTR AMMREVDMRL RQLRHNADVA SPPPPSVPPS CFQTPPTHSS GSAAASPYVL SHPQQQQNFV FTCKEDALQN NFISGGKGRL PTLQNYNLYY TTACFSLEYN RFALDTAPDT LSVNSYNNIA CLFNYALLHN PPQMRYFPPG VPHVRRAEDA LVGYLDHSML WYRFAVREDY DFTLQLIARG LHTLRFRSIA FDVPQMMKVR GGMTDYYRNC HEEIRQQNDR FVLQWPAVAQ HWVKGKEGNR RDDIRVRWDL LSPARAKHPG AFLYLSTPLP QLQPQRLSDD STGGNGGVVS SSDSAATATA VEGAETETRK RLRPPSPTAP TAESSLAPAG RRPCVQAAAT SAPAMSSTAA RDWKGGYVVE RWRDISREEA RQHAGLVALS NDDIHIGRTV AVIPPFFDQK PSVVRATVIE RAVEPTDSHS SSGRSGNASA AEAGDAQQSL TPRVIWTAVA QDVRGMPFLH VDTCFEVPAE GIEKAAASID QFFAKMEGEP GP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttct ccctcaacat caaaacagca gcctcgccgt cctctgcagc tcccgcaccg gcaccggaag tgaacgccgg cgaggcgcca tgggcgatca ttgacgtgga cgaagccgac tcctttgaaa gcacaccctc gacgaacagc agcggtggtc agggcggccg tcatcgttat cagcatgccg cgcagccgtc gcactctccg cagagtcccg agacgcccat caaatttgac cacggtgcat acccgcactg ccccatcttc atcccctcca aaggtcgctc agacctggac cgcagcacca tggcggtgct cgtccgcgat ctcgtcccgt ttgtgcttgt cgtcgagcgt gaggaggccg atgcgtacag agctatgctg gaccgcctcg ttggacaata cttcggcttc gccggtatga cttttgccgc gaacgtggac gatggcattg aaggggcaac caagacgggg gcagaggtcg tcgtgtcgtc cagcactact ccggcagacc tctgggcgtt cccgtgcgcc tgctgctgcg gcagcactga cgtcgctggt tcgccgtcgt cgcccaccac acccggccta tcggaggatg ccttgcgcgc gttggcgctg cggctttcag ctgcgtttca tcatggctat gttttcgagc cgtcagcgtc tgctgcagcc ggcgaccacc acggcgctgt tgagtcgaat gtctcaccgc ctgtgtcgat cagtccaaca gcgccatcag cagccagcgg gagtgcgcgg cgcccctccg cggcctttgt gctgcgcaac gtggacgagg tccgcgcgct cttcgtgatc gaagtgctgc cggagaccaa tcgtggggtc tcctatgtgc gcaactacat cctacaggtt ctcgtgccaa ggctgatggt ggcctgcggc gtagatcggt cagggaacgt ggaggagctg cgcctgtgtg gcacaggaga gcacaacacg ctgacatcgg cggcgctgcg gatggccgcg ctggagccag acgtctacag tgcccttgag tggaagtcag agaccgagtc ctatctggac gcacggcggg cgagcaccac cacgaacgtg gtaacgacaa gcactggcag cgccgcgggt accagagatg gcgtcgtgtc ggcgtcggtg ggagcgttga gcgccccaac cggacaaatt ttggcgccag cgttctcgtc gtcgtctgcg ccacgctcgt caaagccgtc ctccgctgcg gccgcgactc gcggcacgcc tcgccttctg cacggcctct tcggcttcta ctgggtcctg gatgacgaca tctacggctt ttaccagtcg cacggctcca gcaccaaaaa cgagcgcatt agtacgcggg cgatgatgag agaggtggac atgcggctgc ggcaactgcg gcacaacgcc gacgtggcct cgccaccacc gccgtcagtt ccgccttcgt gctttcagac accgccgacc cacagcagcg gcagcgctgc cgcatcgcca tatgtcttgt cacacccgca gcaacagcaa aatttcgtgt tcacgtgcaa ggaggatgcc ctacagaaca atttcatctc tggagggaaa ggccggcttc cgaccctgca gaactacaac ctctactaca caaccgcctg cttctcgctc gagtacaacc gcttcgccct cgacacggct ccggacacgc tgtcggtcaa ctcctacaat aacattgcct gtctcttcaa ctacgcgctg ctgcacaacc cgccgcagat gcgctacttc ccccctgggg tgccgcacgt gaggcgcgcc gaggacgcgc tggtcggcta cctcgatcac agcatgctct ggtaccgctt cgccgtgcgc gaagattacg acttcactct tcagctcatc gcacgtggcc tccacacgct gcgcttccgc agcatcgcct tcgatgtgcc tcagatgatg aaggtgcgtg gcggcatgac ggactactat cgcaactgcc acgaagagat acgacagcag aacgatcgct ttgtgctgca gtggccggcc gtggcgcagc actgggtgaa ggggaaggag ggcaaccgtc gagacgacat acgtgtgcgg tgggacctgc tgagcccagc ccgcgccaag caccccggtg ccttcctgta tctgagcacc ccactgccgc agctgcagcc gcaacgcttg agtgacgaca gcaccggcgg aaacggcggt gtagttagca gcagcgacag tgcagccact gcaaccgcgg tggaaggcgc ggaaacagag acgcgaaagc gtctgcggcc cccgtccccg actgctccca ctgctgaatc gtcacttgcc cctgcaggcc gtcgcccctg cgtccaggcc gcagcaacgt ctgcgcccgc tatgtcgtcc accgctgccc gtgactggaa aggtggctac gtggtcgagc ggtggcgtga tatctcacgt gaggaggcgc ggcagcacgc cggcctcgtc gccctctcca acgatgacat ccacattggc cggactgtcg ctgtcatacc gcccttcttc gaccagaaac catctgtcgt gcgcgcgaca gtgattgagc gggccgtcga gccaacggac tcccattcca gcagcggccg ctctgggaac gccagcgcgg cggaagcagg tgatgcccag cagtccctaa cgccgcgcgt gatctggaca gcggtggcac aagatgttcg tggtatgcct ttcttgcacg ttgacacatg ctttgaggtg cccgcggagg gcatcgaaaa ggccgccgcc agcatcgatc agttcttcgc aaagatggag ggcgagcctg gcccatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for LeinA.19527.a.B2.GE41464

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	79kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHEV NAGEAPWAII DVDEADSFES TPSTNSSGGQ GGRHRYQHAA QPSHSPQSPE TPIKFDHGAY PHCPIFIPSK GRSDLDRSTM AVLVRDLVPF VLVVEREEAD AYRAMLDRLV GQYFGFAGMT FAANVDDGIE GATKTGAEVV VSSSTTPADL WAFPCACCCG STDVAGSPSS PTTPGLSEDA LRALALRLSA AFHHGYVFEP SASAAAGDHH GAVESNVSPP VSISPTAPSA ASGSARRPSA AFVLRNVDEV RALFVIEVLP ETNRGVSYVR NYILQVLVPR LMVACGVDRS GNVEELRLCG TGEHNTLTSA ALRMAALEPD VYSALEWKSE TESYLDARRA STTTNVVTTS TGSAAGTRDG VVSASVGALS APTGQILAPA FSSSSAPRSS KPSSAAAATR GTPRLLHGLF GFYWVLDDDI YGFYQSHGSS TKNERISTRA MMREVDMRLR QLRHNADVAS PPPPSVPPSC FQTPPTHSSG SAAASPYVLS HPQQQQNFVF TCKEDALQNN FISGGKGRLP TLQNYNLYYT TACFSLEYNR FALDTAPDTL SVNSYNNIAC LFNYALLHNP PQMRYFPPGV PHVRRAEDAL VGYLDHSMLW YRFAVREDYD FTLQLIARGL HTLRFRSIAF DVPQMMKVRG GMTDYYRNCH EEIRQQNDRF VLQWPAVAQH WVKGKEGNRR DDIRVRWDLL SPARAKHPGA FLYLSTPLPQ LQPQRLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagtga acgccggcga ggcgccatgg gcgatcattg acgtggacga agccgactcc tttgaaagca caccctcgac gaacagcagc ggtggtcagg gcggccgtca tcgttatcag catgccgcgc agccgtcgca ctctccgcag agtcccgaga cgcccatcaa atttgaccac ggtgcatacc cgcactgccc catcttcatc ccctccaaag gtcgctcaga cctggaccgc agcaccatgg cggtgctcgt ccgcgatctc gtcccgtttg tgcttgtcgt cgagcgtgag gaggccgatg cgtacagagc tatgctggac cgcctcgttg gacaatactt cggcttcgcc ggtatgactt ttgccgcgaa cgtggacgat ggcattgaag gggcaaccaa gacgggggca gaggtcgtcg tgtcgtccag cactactccg gcagacctct gggcgttccc gtgcgcctgc tgctgcggca gcactgacgt cgctggttcg ccgtcgtcgc ccaccacacc cggcctatcg gaggatgcct tgcgcgcgtt ggcgctgcgg ctttcagctg cgtttcatca tggctatgtt ttcgagccgt cagcgtctgc tgcagccggc gaccaccacg gcgctgttga gtcgaatgtc tcaccgcctg tgtcgatcag tccaacagcg ccatcagcag ccagcgggag tgcgcggcgc ccctccgcgg cctttgtgct gcgcaacgtg gacgaggtcc gcgcgctctt cgtgatcgaa gtgctgccgg agaccaatcg tggggtctcc tatgtgcgca actacatcct acaggttctc gtgccaaggc tgatggtggc ctgcggcgta gatcggtcag ggaacgtgga ggagctgcgc ctgtgtggca caggagagca caacacgctg acatcggcgg cgctgcggat ggccgcgctg gagccagacg tctacagtgc ccttgagtgg aagtcagaga ccgagtccta tctggacgca cggcgggcga gcaccaccac gaacgtggta acgacaagca ctggcagcgc cgcgggtacc agagatggcg tcgtgtcggc gtcggtggga gcgttgagcg ccccaaccgg acaaattttg gcgccagcgt tctcgtcgtc gtctgcgcca cgctcgtcaa agccgtcctc cgctgcggcc gcgactcgcg gcacgcctcg ccttctgcac ggcctcttcg gcttctactg ggtcctggat gacgacatct acggctttta ccagtcgcac ggctccagca ccaaaaacga gcgcattagt acgcgggcga tgatgagaga ggtggacatg cggctgcggc aactgcggca caacgccgac gtggcctcgc caccaccgcc gtcagttccg ccttcgtgct ttcagacacc gccgacccac agcagcggca gcgctgccgc atcgccatat gtcttgtcac acccgcagca acagcaaaat ttcgtgttca cgtgcaagga ggatgcccta cagaacaatt tcatctctgg agggaaaggc cggcttccga ccctgcagaa ctacaacctc tactacacaa ccgcctgctt ctcgctcgag tacaaccgct tcgccctcga cacggctccg gacacgctgt cggtcaactc ctacaataac attgcctgtc tcttcaacta cgcgctgctg cacaacccgc cgcagatgcg ctacttcccc cctggggtgc cgcacgtgag gcgcgccgag gacgcgctgg tcggctacct cgatcacagc atgctctggt accgcttcgc cgtgcgcgaa gattacgact tcactcttca gctcatcgca cgtggcctcc acacgctgcg cttccgcagc atcgccttcg atgtgcctca gatgatgaag gtgcgtggcg gcatgacgga ctactatcgc aactgccacg aagagatacg acagcagaac gatcgctttg tgctgcagtg gccggccgtg gcgcagcact gggtgaaggg gaaggagggc aaccgtcgag acgacatacg tgtgcggtgg gacctgctga gcccagcccg cgccaagcac cccggtgcct tcctgtatct gagcacccca ctgccgcagc tgcagccgca acgcttgagt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for LeinA.19527.a.B3.GE41465

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	16kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD WKGGYVVERW RDISREEARQ HAGLVALSND DIHIGRTVAV IPPFFDQKPS VVRATVIERA VEPTDSHSSS GRSGNASAAE AGDAQQSLTP RVIWTAVAQD VRGMPFLHVD TCFEVPAEGI EKAAASIDQF FAKMEGEPGP

Validated NT Sequence

atggctcacc accaccacca ccatatggac tggaaaggtg gctacgtggt cgagcggtgg cgtgatatct cacgtgagga ggcgcggcag cacgccggcc tcgtcgccct ctccaacgat gacatccaca ttggccggac tgtcgctgtc ataccgccct tcttcgacca gaaaccatct gtcgtgcgcg cgacagtgat tgagcgggcc gtcgagccaa cggactccca ttccagcagc ggccgctctg ggaacgccag cgcggcggaa gcaggtgatg cccagcagtc cctaacgccg cgcgtgatct ggacagcggt ggcacaagat gttcgtggta tgcctttctt gcacgttgac acatgctttg aggtgcccgc ggagggcatc gaaaaggccg ccgccagcat cgatcagttc ttcgcaaaga tggagggcga gcctggccca taagtgagta agat

Expected Protein Sequence

MAHHHHHHDW KGGYVVERWR DISREEARQH AGLVALSNDD IHIGRTVAVI PPFFDQKPSV VRATVIERAV EPTDSHSSSG RSGNASAAEA GDAQQSLTPR VIWTAVAQDV RGMPFLHVDT CFEVPAEGIE KAAASIDQFF AKMEGEPGP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgactgga aaggtggcta cgtggtcgag cggtggcgtg atatctcacg tgaggaggcg cggcagcacg ccggcctcgt cgccctctcc aacgatgaca tccacattgg ccggactgtc gctgtcatac cgcccttctt cgaccagaaa ccatctgtcg tgcgcgcgac agtgattgag cgggccgtcg agccaacgga ctcccattcc agcagcggcc gctctgggaa cgccagcgcg gcggaagcag gtgatgccca gcagtcccta acgccgcgcg tgatctggac agcggtggca caagatgttc gtggtatgcc tttcttgcac gttgacacat gctttgaggt gcccgcggag ggcatcgaaa aggccgccgc cagcatcgat cagttcttcg caaagatgga gggcgagcct ggcccatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LeinA.19527.a.B2.PS38314

PURIFICATION DATe:	7/7/2017
CONCENTRATION:	47.3mg/ml
OBSERVED MW:	80kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAAGTGA ACGCCGGCGA GGCGCCATGG GCGATCATTG ACGTGGACGA AGCCGACTCC TTTGAAAGCA CACCCTCGAC GAACAGCAGC GGTGGTCAGG GCGGCCGTCA TCGTTATCAG CATGCCGCGC AGCCGTCGCA CTCTCCGCAG AGTCCCGAGA CGCCCATCAA ATTTGACCAC GGTGCATACC CGCACTGCCC CATCTTCATC CCCTCCAAAG GTCGCTCAGA CCTGGACCGC AGCACCATGG CGGTGCTCGT CCGCGATCTC GTCCCGTTTG TGCTTGTCGT CGAGCGTGAG GAGGCCGATG CGTACAGAGC TATGCTGGAC CGCCTCGTTG GACAATACTT CGGCTTCGCC GGTATGACTT TTGCCGCGAA CGTGGACGAT GGCATTGAAG GGGCAACCAA GACGGGGGCA GAGGTCGTCG TGTCGTCCAG CACTACTCCG GCAGACCTCT GGGCGTTCCC GTGCGCCTGC TGCTGCGGCA GCACTGACGT CGCTGGTTCG CCGTCGTCGC CCACCACACC CGGCCTATCG GAGGATGCCT TGCGCGCGTT GGCGCTGCGG CTTTCAGCTG CGTTTCATCA TGGCTATGTT TTCGAGCCGT CAGCGTCTGC TGCAGCCGGC GACCACCACG GCGCTGTTGA GTCGAATGTC TCACCGCCTG TGTCGATCAG TCCAACAGCG CCATCAGCAG CCAGCGGGAG TGCGCGGCGC CCCTCCGCGG CCTTTGTGCT GCGCAACGTG GACGAGGTCC GCGCGCTCTT CGTGATCGAA GTGCTGCCGG AGACCAATCG TGGGGTCTCC TATGTGCGCA ACTACATCCT ACAGGTTCTC GTGCCAAGGC TGATGGTGGC CTGCGGCGTA GATCGGTCAG GGAACGTGGA GGAGCTGCGC CTGTGTGGCA CAGGAGAGCA CAACACGCTG ACATCGGCGG CGCTGCGGAT GGCCGCGCTG GAGCCAGACG TCTACAGTGC CCTTGAGTGG AAGTCAGAGA CCGAGTCCTA TCTGGACGCA CGGCGGGCGA GCACCACCAC GAACGTGGTA ACGACAAGCA CTGGCAGCGC CGCGGGTACC AGAGATGGCG TCGTGTCGGC GTCGGTGGGA GCGTTGAGCG CCCCAACCGG ACAAATTTTG GCGCCAGCGT TCTCGTCGTC GTCTGCGCCA CGCTCGTCAA AGCCGTCCTC CGCTGCGGCC GCGACTCGCG GCACGCCTCG CCTTCTGCAC GGCCTCTTCG GCTTCTACTG GGTCCTGGAT GACGACATCT ACGGCTTTTA CCAGTCGCAC GGCTCCAGCA CCAAAAACGA GCGCATTAGT ACGCGGGCGA TGATGAGAGA GGTGGACATG CGGCTGCGGC AACTGCGGCA CAACGCCGAC GTGGCCTCGC CACCACCGCC GTCAGTTCCG CCTTCGTGCT TTCAGACACC GCCGACCCAC AGCAGCGGCA GCGCTGCCGC ATCGCCATAT GTCTTGTCAC ACCCGCAGCA ACAGCAAAAT TTCGTGTTCA CGTGCAAGGA GGATGCCCTA CAGAACAATT TCATCTCTGG AGGGAAAGGC CGGCTTCCGA CCCTGCAGAA CTACAACCTC TACTACACAA CCGCCTGCTT CTCGCTCGAG TACAACCGCT TCGCCCTCGA CACGGCTCCG GACACGCTGT CGGTCAACTC CTACAATAAC ATTGCCTGTC TCTTCAACTA CGCGCTGCTG CACAACCCGC CGCAGATGCG CTACTTCCCC CCTGGGGTGC CGCACGTGAG GCGCGCCGAG GACGCGCTGG TCGGCTACCT CGATCACAGC ATGCTCTGGT ACCGCTTCGC CGTGCGCGAA GATTACGACT TCACTCTTCA GCTCATCGCA CGTGGCCTCC ACACGCTGCG CTTCCGCAGC ATCGCCTTCG ATGTGCCTCA GATGATGAAG GTGCGTGGCG GCATGACGGA CTACTATCGC AACTGCCACG AAGAGATACG ACAGCAGAAC GATCGCTTTG TGCTGCAGTG GCCGGCCGTG GCGCAGCACT GGGTGAAGGG GAAGGAGGGC AACCGTCGAG ACGACATACG TGTGCGGTGG GACCTGCTGA GCCCAGCCCG CGCCAAGCAC CCCGGTGCCT TCCTGTATCT GAGCACCCCA CTGCCGCAGC TGCAGCCGCA ACGCTTGAGT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT