LeinA.19526.a

WDR82 homologue (glucosyl transferase interacting protein 1) (GTIP1)

CENTER ID: LeinA.19526.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.19526.a.B2.GE41462 WD-40 propeller domain 25 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79398
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.32.3970
UniProt: A4I8K0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSAAPSPP FTSSGVAAGS AAVDGVPIEV ASMFYTGINE PRPTAMNFSF DGLHFVSAHT DDAVRLIDVG NMTNTDVIIC DAFGVHNVRY THSSSVVCVA PRFHLDGHLH LLNTETSQFF GEMAYINDAE SEMLPVANTP VYSTLAQCPA TDVLGAVVVA KGRLALFHPL ISGCIAATPE RSIVGSRACL QFSQDGHRIA IGDDHRIVLF DRRSLFRAPL AVLENKHVFT TSTSLTRCKG LEFSADASRL LLTSSSGEVV VYDWLRGEAV ANYFHGDARR HFVGSADAIG AQHAFPYQAA SPILQLTSSM TGGRHLLVYA GCPSNSAAST RQGRLLYELQ SKDSDVPVAI AVNPRLQLAA TAARSITWWA FHSSVVEAYA TLTEAGTTAA D
NT Sequence
atgagcagct ccgccgcccc gagccctccc ttcacctcca gcggtgtagc tgctggctcg gctgctgtgg acggagtacc gatagaggtg gcgtccatgt tctacacagg catcaacgaa ccacggccca ccgcgatgaa cttctccttc gacggccttc acttcgtgtc ggcgcacacg gacgacgccg tccgactcat cgatgtgggc aacatgacga acacggacgt gattatctgc gacgcgtttg gcgtgcacaa cgtccgttac acccattcca gcagtgtcgt gtgtgtcgcg ccgcggtttc acctggatgg ccacctgcac ctcctgaaca cggagacatc gcagttcttc ggcgagatgg cgtacatcaa cgacgccgag tcggagatgc tgccagtggc gaacacgcca gtgtactcca cccttgccca gtgcccggca acagacgtgc tcggtgccgt ggtggtggcc aaaggtcggc tagccctctt tcaccctctc atctctggtt gcattgcggc cacgccggag cggtccattg taggcagtag agcctgcttg cagtttagcc aagacggcca tcggatcgcc atcggcgacg accaccgcat cgttttattc gatcgacgca gcctcttccg ggcaccgcta gccgtgctcg agaacaagca tgtctttacc acgtcgacgt cgctgacgag atgcaaaggg ctcgagttta gcgccgatgc gagtcgcctg ctgctcacat cgtcgagtgg tgaggttgtg gtatacgact ggctgcgcgg cgaggcggtg gcgaactact ttcacggcga cgcgaggcgg cactttgtcg gctctgccga cgccatcggc gcacagcacg ccttccctta ccaggcggcc tcgcccattc tgcagctcac ttcgtccatg accggaggcc ggcatctgct cgtatatgcc gggtgcccgt ccaactcggc ggcctccacc cgacaaggac gacttctgta tgagctgcag tcgaaggaca gcgatgtgcc ggttgctatt gccgtgaacc cgcgactaca gctagcggcc accgcagcgc ggagtataac gtggtgggct tttcacagct ctgtagtgga ggcgtacgcg acactgacgg aggctggcac gactgcagcc gactga
Details for LeinA.19526.a.B2.GE41462
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG VPIEVASMFY TGINEPRPTA MNFSFDGLHF VSAHTDDAVR LIDVGNMTNT DVIICDAFGV HNVRYTHSSS VVCVAPRFHL DGHLHLLNTE TSQFFGEMAY INDAESEMLP VANTPVYSTL AQCPATDVLG AVVVAKGRLA LFHPLISGCI AATPERSIVG SRACLQFSQD GHRIAIGDDH RIVLFDRRSL FRAPLAVLEN KHVFTTSTSL TRCKGLEFSA DASRLLLTSS SGEVVVYDWL RGEAVANYFH GDARRHFVGS ADAIGAQHAF PYQAASPILQ LTSSMTGGRH LLVYAGCPSN SAASTRQGRL LYELQSKDSD VPVAIAVNPR LQLAATAARS
Validated NT Sequence
atggctcacc accaccacca ccatatggga gtaccgatag aggtggcgtc catgttctac acaggcatca acgaaccacg gcccaccgcg atgaacttct ccttcgacgg ccttcacttc gtgtcggcgc acacggacga cgccgtccga ctcatcgatg tgggcaacat gacgaacacg gacgtgatta tctgcgacgc gtttggcgtg cacaacgtcc gttacaccca ttccagcagt gtcgtgtgtg tcgcgccgcg gtttcacctg gatggccacc tgcacctcct gaacacggag acatcgcagt tcttcggcga gatggcgtac atcaacgacg ccgagtcgga gatgctgcca gtggcgaaca cgccagtgta ctccaccctt gcccagtgcc cggcaacaga cgtgctcggt gccgtggtgg tggccaaagg tcggctagcc ctctttcacc ctctcatctc tggttgcatt gcggccacgc cggagcggtc cattgtaggc agtagagcct gcttgcagtt tagccaagac ggccatcgga tcgccatcgg cgacgaccac cgcatcgttt tattcgatcg acgcagcctc ttccgggcac cgctagccgt gctcgagaac aagcatgtct ttaccacgtc gacgtcgctg acgaggtgca aagggctcga gtttagcgcc gatgcgagtc gcctgctgct cacatcgtcg agtggtgagg ttgtggtata cgactggctg cgcggcgagg cggtggcgaa ctactttcac ggcgacgcga ggcggcactt tgtcggctct gccgacgcca tcggcgcaca gcacgccttc ccttaccagg cggcctcgcc cattctgcag ctcacttcgt ccatgaccgg aggccggcat ctgctcgtat atgccgggtg cccgtccaac tcggcggcct ccacccgaca aggacgactt ctgtatgagc tgcagtcgaa ggacagcgat gtgccggttg ctattgccgt gaacccgcga ctacagctag cggccaccgc agcgcggagt at
Expected Protein Sequence
MAHHHHHHGV PIEVASMFYT GINEPRPTAM NFSFDGLHFV SAHTDDAVRL IDVGNMTNTD VIICDAFGVH NVRYTHSSSV VCVAPRFHLD GHLHLLNTET SQFFGEMAYI NDAESEMLPV ANTPVYSTLA QCPATDVLGA VVVAKGRLAL FHPLISGCIA ATPERSIVGS RACLQFSQDG HRIAIGDDHR IVLFDRRSLF RAPLAVLENK HVFTTSTSLT RCKGLEFSAD ASRLLLTSSS GEVVVYDWLR GEAVANYFHG DARRHFVGSA DAIGAQHAFP YQAASPILQL TSSMTGGRHL LVYAGCPSNS AASTRQGRLL YELQSKDSDV PVAIAVNPRL QLAATAARSI TWWAFHS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggagtac cgatagaggt ggcgtccatg ttctacacag gcatcaacga accacggccc accgcgatga acttctcctt cgacggcctt cacttcgtgt cggcgcacac ggacgacgcc gtccgactca tcgatgtggg caacatgacg aacacggacg tgattatctg cgacgcgttt ggcgtgcaca acgtccgtta cacccattcc agcagtgtcg tgtgtgtcgc gccgcggttt cacctggatg gccacctgca cctcctgaac acggagacat cgcagttctt cggcgagatg gcgtacatca acgacgccga gtcggagatg ctgccagtgg cgaacacgcc agtgtactcc acccttgccc agtgcccggc aacagacgtg ctcggtgccg tggtggtggc caaaggtcgg ctagccctct ttcaccctct catctctggt tgcattgcgg ccacgccgga gcggtccatt gtaggcagta gagcctgctt gcagtttagc caagacggcc atcggatcgc catcggcgac gaccaccgca tcgttttatt cgatcgacgc agcctcttcc gggcaccgct agccgtgctc gagaacaagc atgtctttac cacgtcgacg tcgctgacga gatgcaaagg gctcgagttt agcgccgatg cgagtcgcct gctgctcaca tcgtcgagtg gtgaggttgt ggtatacgac tggctgcgcg gcgaggcggt ggcgaactac tttcacggcg acgcgaggcg gcactttgtc ggctctgccg acgccatcgg cgcacagcac gccttccctt accaggcggc ctcgcccatt ctgcagctca cttcgtccat gaccggaggc cggcatctgc tcgtatatgc cgggtgcccg tccaactcgg cggcctccac ccgacaagga cgacttctgt atgagctgca gtcgaaggac agcgatgtgc cggttgctat tgccgtgaac ccgcgactac agctagcggc caccgcagcg cggagtataa cgtggtgggc ttttcacagc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat