LeinA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID: LeinA.19501.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.19501.a.B2.GE40682 C-term domain 384 657
LeinA.19501.a.B3.GE40683 J-binding domain 113 288
LeinA.19501.a.B4.GE41027 N-extended J-binding domain 83 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.19501.a.B4.PW38194 N-extended J-binding domain 83 284
Molecular models
METHOD RESULTS
comparative modelling Robetta_79388
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.36.0420
UniProt: A4ICU8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSKRTEGTA ADEERRQTSA ASSGSSSVSS TDSEEASSSV SDSSSSASGS SSGSESSDSE RPLSPPQRQS AAATERATST QQLQQQRHVQ LNPELRFERP DKEYIPLPPL RDMQDMAKVL FLLSTDKKRY PDGRHRTLDY FRVAVEMFVT EVRQEYKRQY QQAQRSGRVM QRFTWKNSGE LAICFACCCD NVKLLYDSLQ PGPLKPLWDA FVGQLAPMLI VQSRVPETML SSQTYHTKYM DWVKGGTRYG GEQSTANVRF PSVADRRVKV ETYLRSGAGY KIEAVQVGST SAAPATVPAA VAPRPSLAAA AAPMQALETR APFAGRLPGT GSVKVPVAPG VGGPRPPVKV PMKHAGGGVA HGSVTAAAAP PLASTSVKQM PPEMKHLFWL SVLAHVKGGE ASRAFPDDRL EALPGLLRLC EMTEHTEEMC ILMDVLAASS SGVQTVFERL GGLLSFRRFA SHFIQSEEAE PLMQLVEQLA RWRVAAWGST SQQAWQTDMN QRRRRDLMWL RTLPLIPEAK RAAWGAVLLV MEQRYIFKAP RPDDTTTADN PLKRRRPEVE LGDLHAVLSD NVDMQRWVVP RVNVFSAVRL PVELRVPPLC TKLEEDTVRF LNSKREYAEK AQVTWRERLE KILSAQPRAP GAAVPVPLWY DPYALLSVAS PQL
NT Sequence
atgtcctcca aacgcaccga gggcacggct gctgacgagg agcgccggca gacgtcggcc gcgtctagcg gctcgtcgag cgtcagcagt accgactctg aagaagccag cagcagtgtc tccgacagca gcagcagcgc cagcggcagc tcctccggct ccgaatcgtc tgatagcgaa cgtccgttgt cccccccgca gcgccaaagt gctgctgcaa cagagagagc cacgtctacg cagcagctac aacagcagcg gcatgtgcag ctgaatcccg agctgcgctt cgagcggcca gacaaggagt acatcccgct accacccctc agagacatgc aggacatggc caaggttctg tttctcttga gcaccgataa gaaacgctat ccggatgggc gtcaccgcac gctggactac ttccgtgtcg cggtggagat gtttgtgacg gaggtgcggc aagagtacaa acggcagtac cagcaggcgc agcgcagcgg ccgtgtgatg cagcggttca cgtggaagaa ctcgggcgag ctggcgattt gctttgcctg ctgctgcgac aatgtgaagc ttctgtatga cagcctccaa cctggccccc tcaaaccact ctgggacgcc tttgtcggcc agctcgcgcc gatgctcatc gttcagagcc gcgtgccgga gacgatgctg tcatcacaaa cgtaccacac caagtacatg gactgggtga agggcggcac acgttacggt ggcgagcagt cgaccgcgaa tgtccgcttc ccctctgtgg cagaccgtcg cgtaaaggtt gaaacatacc tccggagcgg cgcggggtac aagatcgagg ctgtgcaggt ggggtcgacg tccgccgcgc cggcgaccgt ccctgccgcg gtggcgcctc gcccttcttt ggccgctgcc gcggctccga tgcaggcttt agagactcgg gcgcctttcg cggggcggct gcccggcaca ggttcggtga aggtgcccgt cgctccaggt gtcggtggac ctcgcccgcc agtgaaggtt cccatgaagc acgccggcgg cggtgttgcg cacggcagtg tgacggccgc cgccgctcct ccgcttgcct ccaccagtgt gaagcagatg ccgccggaga tgaagcatct gttttggttg tcggtgctgg cgcacgtgaa gggcggagaa gcgtctcggg cgtttccgga tgaccgactg gaggcgctgc ctggcctgct acggctgtgc gagatgacag agcacacgga ggagatgtgc atcttgatgg atgtgctggc ggcctcgagc agcggcgttc agacagtctt cgagcgtctt ggcggccttc tgtcattccg ccggttcgcc tcccacttca tccagagtga ggaggcagag ccgctcatgc aactcgtcga gcagctcgcg cggtggcgcg tagcggcatg gggcagtacc tcacagcagg catggcagac cgatatgaac caacggcgca gacgcgatct gatgtggctc cgtaccctgc ccctcatccc ggaggcgaag cgggcggcgt ggggggcggt gcttctggtg atggagcaac gctatatttt caaggccccg cgccctgatg acacgaccac cgctgacaat ccactgaagc ggcggcgacc cgaggtcgag ctcggcgatc tgcacgctgt gctgagtgac aacgtcgaca tgcaaaggtg ggtggtaccc cgggtcaatg tgttctccgc tgttcgccta ccagtggagc tgcgcgtgcc gccactgtgc acaaagttgg aggaggatac tgtccgcttc ctcaactcca agcgcgagta cgccgagaag gcgcaggtga cgtggcgtga gaggctagag aagattctga gcgcgcagcc tcgtgctccg ggtgctgccg tgccggtgcc tctctggtat gacccttacg cgctactgag cgtggcctct ccgcaactat ga
Details for LeinA.19501.a.B2.GE40682
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK HLFWLSVLAH VKGGEASRAF PDDRLEALPG LLRLCEMTEH TEEMCILMDV LAASSSGVQT VFERLGGLLS FRRFASHFIQ SEEAEPLMQL VEQLARWRVA AWGSTSQQAW QTDMNQRRTR DLMWLRTLPL IPEAKRAAWG AVLLVMEQRY IFKAPRPDDT TTADNPLKRR RPEVELGDLH AVLSDNVDMQ RWVVPRVNVF SAVRLPVELR VPPLCTKLEE DTVRFLNSKR EYAEKAQVTW RERLEKILSA QPRAPGAAVP VPLWXXXXAL LS
Validated NT Sequence
atggctcacc accaccacca ccatatgaag catctgtttt ggttgtcggt gctggcgcac gtgaagggcg gagaagcgtc tcgggcgttt ccggatgacc gactggaggc gctgcctggc ctgctacggc tgtgcgagat gacagagcac acggaggaga tgtgcatctt gatggatgtg ctggcggcct cgagcagcgg cgttcagaca gtcttcgagc gtcttggcgg ccttctgtca ttccgccggt tcgcctccca cttcatccag agtgaggagg cagagccgct catgcaactc gtcgagcagc tcgcgcggtg gcgcgtagcg gcatggggca gtacctcaca gcaggcgtgg cagaccgata tgaaccaacg gcgcacacgc gatctgatgt ggctccgtac cctgcccctc atcccggagg cgaagcgggc ggcgtggggg gcggtgcttc tggtgatgga gcaacgctat attttcaagg ccccgcgccc tgatgacacg accaccgctg acaatccact gaagcggcgg cgacccgagg tcgagctcgg cgatctgcac gctgtgctga gtgacaacgt cgacatgcaa aggtgggtgg taccccgggt caatgtgttc tccgctgttc gcctaccagt ggagctgcgc gtgccgccac tgtgcacaaa gttggaggag gatactgtcc gcttcctcaa ctccaagcgc gagtacgccg agaaggcgca ggtgacgtgg cgtgagaggc tagagaagat tctgagcgcg cagcctcgtg ctccgggtgc tgccgtgccg gtgcctctct ggtnnnnnnn nnacgcgcta ctgagctaag tgagtaagat
Expected Protein Sequence
MAHHHHHHMK HLFWLSVLAH VKGGEASRAF PDDRLEALPG LLRLCEMTEH TEEMCILMDV LAASSSGVQT VFERLGGLLS FRRFASHFIQ SEEAEPLMQL VEQLARWRVA AWGSTSQQAW QTDMNQRRRR DLMWLRTLPL IPEAKRAAWG AVLLVMEQRY IFKAPRPDDT TTADNPLKRR RPEVELGDLH AVLSDNVDMQ RWVVPRVNVF SAVRLPVELR VPPLCTKLEE DTVRFLNSKR EYAEKAQVTW RERLEKILSA QPRAPGAAVP VPLWYDPYAL LS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc atctgttttg gttgtcggtg ctggcgcacg tgaagggcgg agaagcgtct cgggcgtttc cggatgaccg actggaggcg ctgcctggcc tgctacggct gtgcgagatg acagagcaca cggaggagat gtgcatcttg atggatgtgc tggcggcctc gagcagcggc gttcagacag tcttcgagcg tcttggcggc cttctgtcat tccgccggtt cgcctcccac ttcatccaga gtgaggaggc agagccgctc atgcaactcg tcgagcagct cgcgcggtgg cgcgtagcgg catggggcag tacctcacag caggcatggc agaccgatat gaaccaacgg cgcagacgcg atctgatgtg gctccgtacc ctgcccctca tcccggaggc gaagcgggcg gcgtgggggg cggtgcttct ggtgatggag caacgctata ttttcaaggc cccgcgccct gatgacacga ccaccgctga caatccactg aagcggcggc gacccgaggt cgagctcggc gatctgcacg ctgtgctgag tgacaacgtc gacatgcaaa ggtgggtggt accccgggtc aatgtgttct ccgctgttcg cctaccagtg gagctgcgcg tgccgccact gtgcacaaag ttggaggagg atactgtccg cttcctcaac tccaagcgcg agtacgccga gaaggcgcag gtgacgtggc gtgagaggct agagaagatt ctgagcgcgc agcctcgtgc tccgggtgct gccgtgccgg tgcctctctg gtatgaccct tacgcgctac tgagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LeinA.19501.a.B3.GE40683
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ DMAKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYKRQYQQAQ RSGRVMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LAPMLIVQSR VPETMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA VQVG
Validated NT Sequence
atggctcacc accaccacca ccatatgcag gacatggcca aggttctgtt tctcttgagc accgataaga aacgctatcc ggatgggcgt caccgcacgc tggactactt ccgtgtcgcg gtggagatgt ttgtgacgga ggtgcggcag gagtacaaac ggcagtacca gcaggcgcag cgcagcggcc gtgtgatgca gcggttcacg tggaagaact cgggcgagct ggcgatttgc tttgcctgct gctgcgacaa tgtgaagctt ctgtacgaca gcctccaacc tggccccctc aaaccactct gggacgcctt tgtcggccag ctcgcgccga tgctcatcgt tcagagccgc gtgccggaga cgatgctgtc atcacaaacg taccacacca agtacatgga ctgggtgaag ggcggcacac gttacggtgg cgagcagtcg accgcgaatg tccgcttccc ctctgtggca gaccgtcgcg taaaggttga aacatacctt cggagcggcg cggggtacaa gatcgaggct gtgcaggtgg ggtaagtgag taagat
Expected Protein Sequence
MAHHHHHHMQ DMAKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYKRQYQQAQ RSGRVMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LAPMLIVQSR VPETMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA VQVG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagg acatggccaa ggttctgttt ctcttgagca ccgataagaa acgctatccg gatgggcgtc accgcacgct ggactacttc cgtgtcgcgg tggagatgtt tgtgacggag gtgcggcaag agtacaaacg gcagtaccag caggcgcagc gcagcggccg tgtgatgcag cggttcacgt ggaagaactc gggcgagctg gcgatttgct ttgcctgctg ctgcgacaat gtgaagcttc tgtatgacag cctccaacct ggccccctca aaccactctg ggacgccttt gtcggccagc tcgcgccgat gctcatcgtt cagagccgcg tgccggagac gatgctgtca tcacaaacgt accacaccaa gtacatggac tgggtgaagg gcggcacacg ttacggtggc gagcagtcga ccgcgaatgt ccgcttcccc tctgtggcag accgtcgcgt aaaggttgaa acatacctcc ggagcggcgc ggggtacaag atcgaggctg tgcaggtggg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for LeinA.19501.a.B4.GE41027
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML QQQRHVQLNP ELRFERPDKE YIPLPPLRDM QDMAKVLFLL STDKKRYPDG RHRTLDYFRV AVEMFVTEVR QEYKRQYQQA QRSGRVMQRF TWKNSGELAI CFACCCDNVK LLYDSLQPGP LKPLWDAFVG QLAPMLIVQS RVPETMLSSQ TYHTKYMDWV KGGTRYGGEQ STANVRFPSV ADRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atcttactca cttaagcctc gatcttgtac cccgcgccgc tccgaaggta tgtttcaacc tttacgcgac ggtctgccac agaggggaag cggacattcg cggtcgactg ctcgccaccg taacgtgtgc cgcccttcac ccagtccatg tacttggtgt ggtacgtttg tgatgacagc atcgtctccg gcacgcggct ctgaacgatg agcatcggcg cgagctggcc gacaaaggcg tcccagagtg gtttgagggg gccaggttgg aggctgtcgt acagaagctt cacattgtcg cagcagcagg caaagcaaat cgccagctcg cccgagttct tccacgtgaa ccgctgcatc acacggccgc tgcgctgcgc ctgctggtac tgccgtttgt actcctgccg cacctccgtc acaaacatct ccaccgcgac acggaagtag tccagcgtgc ggtgacgccc atccggatag cgtttcttat cggtgctcaa gagaaacaga accttggcca tgtcctgcat gtctctgagg ggtggtagcg ggatgtactc cttgtctggc cgctcgaagc gcagctcggg attcagctgc acatgccgct gctgttgtag catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHLQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMAKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYKRQYQQAQ RSGRVMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LAPMLIVQSR VPETMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctacaac agcagcggca tgtgcagctg aatcccgagc tgcgcttcga gcggccagac aaggagtaca tcccgctacc acccctcaga gacatgcagg acatggccaa ggttctgttt ctcttgagca ccgataagaa acgctatccg gatgggcgtc accgcacgct ggactacttc cgtgtcgcgg tggagatgtt tgtgacggag gtgcggcaag agtacaaacg gcagtaccag caggcgcagc gcagcggccg tgtgatgcag cggttcacgt ggaagaactc gggcgagctg gcgatttgct ttgcctgctg ctgcgacaat gtgaagcttc tgtatgacag cctccaacct ggccccctca aaccactctg ggacgccttt gtcggccagc tcgcgccgat gctcatcgtt cagagccgcg tgccggagac gatgctgtca tcacaaacgt accacaccaa gtacatggac tgggtgaagg gcggcacacg ttacggtggc gagcagtcga ccgcgaatgt ccgcttcccc tctgtggcag accgtcgcgt aaaggttgaa acatacctcc ggagcggcgc ggggtacaag atcgaggctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LeinA.19501.a.B4.PW38194
PURIFICATION DATe: 2/28/2017
CONCENTRATION: 42mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML QQQRHVQLNP ELRFERPDKE YIPLPPLRDM QDMAKVLFLL STDKKRYPDG RHRTLDYFRV AVEMFVTEVR QEYKRQYQQA QRSGRVMQRF TWKNSGELAI CFACCCDNVK LLYDSLQPGP LKPLWDAFVG QLAPMLIVQS RVPETMLSSQ TYHTKYMDWV KGGTRYGGEQ STANVRFPSV ADRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atcttactca cttaagcctc gatcttgtac cccgcgccgc tccgaaggta tgtttcaacc tttacgcgac ggtctgccac agaggggaag cggacattcg cggtcgactg ctcgccaccg taacgtgtgc cgcccttcac ccagtccatg tacttggtgt ggtacgtttg tgatgacagc atcgtctccg gcacgcggct ctgaacgatg agcatcggcg cgagctggcc gacaaaggcg tcccagagtg gtttgagggg gccaggttgg aggctgtcgt acagaagctt cacattgtcg cagcagcagg caaagcaaat cgccagctcg cccgagttct tccacgtgaa ccgctgcatc acacggccgc tgcgctgcgc ctgctggtac tgccgtttgt actcctgccg cacctccgtc acaaacatct ccaccgcgac acggaagtag tccagcgtgc ggtgacgccc atccggatag cgtttcttat cggtgctcaa gagaaacaga accttggcca tgtcctgcat gtctctgagg ggtggtagcg ggatgtactc cttgtctggc cgctcgaagc gcagctcggg attcagctgc acatgccgct gctgttgtag catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHLQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMAKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYKRQYQQAQ RSGRVMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LAPMLIVQSR VPETMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTACAAC AGCAGCGGCA TGTGCAGCTG AATCCCGAGC TGCGCTTCGA GCGGCCAGAC AAGGAGTACA TCCCGCTACC ACCCCTCAGA GACATGCAGG ACATGGCCAA GGTTCTGTTT CTCTTGAGCA CCGATAAGAA ACGCTATCCG GATGGGCGTC ACCGCACGCT GGACTACTTC CGTGTCGCGG TGGAGATGTT TGTGACGGAG GTGCGGCAAG AGTACAAACG GCAGTACCAG CAGGCGCAGC GCAGCGGCCG TGTGATGCAG CGGTTCACGT GGAAGAACTC GGGCGAGCTG GCGATTTGCT TTGCCTGCTG CTGCGACAAT GTGAAGCTTC TGTATGACAG CCTCCAACCT GGCCCCCTCA AACCACTCTG GGACGCCTTT GTCGGCCAGC TCGCGCCGAT GCTCATCGTT CAGAGCCGCG TGCCGGAGAC GATGCTGTCA TCACAAACGT ACCACACCAA GTACATGGAC TGGGTGAAGG GCGGCACACG TTACGGTGGC GAGCAGTCGA CCGCGAATGT CCGCTTCCCC TCTGTGGCAG ACCGTCGCGT AAAGGTTGAA ACATACCTCC GGAGCGGCGC GGGGTACAAG ATCGAGGCTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT