LeinA.18771.a

leucyl-tRNA synthetase, putative

CENTER ID: LeinA.18771.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18771.a.B2.GE43059 XtalPred domain 50 931
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_88904
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.13.1000
UniProt: A4HVN8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTARRDALV AIEQEKQAYW AAEKLHEFDA PAPGEARPAK YLTTFPFPYM NGKLHLGHGF SLTKAEFASR FQRMMGRRSL WPFGFHVTGT PIAACAQKIA KEMQQYGNPP QFPAELLEDK PKAMVVKEPT EALGQHKSKR GKSGPAKPQW LIMQSMGIPD SEIAKFADPQ YWLDYFPPIA MEDLKSFGCH IDWRRSFMTT ERSPYFDRFV RWQFNILRRE NLLNYGKRYC VYSPRDGQPC ADHDRASGEG VLPQEYALVK LIVQNPLAQP CFAEHKDIIG DRNVVLPGAT LRPETVIGQT NCWVSNKLNY KGYYVRNAKG EDEVYIMTAR AARNIAYQNF YVNGQVGTDP EPLFEVEGAS MVGIPLSAPL APYTTIYTLP MATITEAKGT GVVMSVPSDS PDDYINFTQL LNKPEYRVKL GIKDEWVVPF AMIPIIDIPE LGTEGAKFMC EKLKINGPNA TELLEEAKKV CYQQGFYHGT MISGPYAGVK VSEAKVKTHR DMEAADQCIR YYEPVRQVIS RSGDECVVAL CDQWYLEYGK DDWKKLVQDH IKKMDMFFPG VRNGFEETLN WLADWPCSRT FGLGTYLPCD ASHTMIIDSL SDSTIYMAYY TIDRFFNVGA DGSTDLCGKA DNPYGLAPEM FTDEVFEYIY HGVGDAATVA GAVRMPVESL KLMRNEFEYW YPVDLRCSGK DLIQNHLTMF LYNHAAIWPT DESKWPRAIF CNGHIQVDNE KMAKSRGNFI SLREAMDMYG ADATRLTCAD AGDSMDDANF VRETAAGFVL KMTTLLEQAK ETVERTDLRS GGYNEFDKIF NNTMNTIITR TEGFYHRMQF RMVLNTAFHE LSNEYSQYKM YCDDLNVHAQ LGRRYYEVVA LMMMPLAPHT MEHLWQNILQ HEGSVVTQPF PKPTAKLDYS LIVASRVMTI ALKEIRSQVI KNAKKRGPVE EVIVYTRREY TDWQRQALEI LHELYEANGN TFPEDMAKQV VARDTKIMSK ALMAFMSFVK GNVEKYGEQA MSTQPVIDDA VMLSNVTHNL SRLAGVSVVR ILSAEDETYK EHDAARRKCL PGEPSVALPP VVKRE
NT Sequence
atgtccacgg cacgtcgcga tgcgctcgtg gcgattgagc aggagaagca agcctactgg gccgcggaga agttgcatga gttcgacgcc ccagcgcctg gggaggcgcg accggcgaag tatctgacca cgttcccctt cccgtacatg aacggcaagc ttcacctcgg tcatggcttc tcgctcacga aggccgagtt cgcctcgcgc ttccagcgca tgatgggccg ccgttcgctc tggcccttcg gcttccacgt caccggcacc ccgatcgctg cgtgcgcgca gaaaatcgcc aaggagatgc agcagtacgg caacccgccg cagttcccgg cggagctgct cgaggacaag cccaaggcga tggtggtcaa ggagccgacg gaggcgctag ggcagcacaa gagcaagcgc ggcaagagcg ggccggcgaa accgcagtgg ctcatcatgc agagcatggg catccccgac tcggagatcg cgaagttcgc cgacccgcag tactggctgg actacttccc gcccatcgcg atggaggact tgaagagttt tggctgccac atcgactggc gccgctcctt catgacgacg gagcgtagcc catactttga ccgcttcgtg cggtggcagt tcaacatcct gcgccgcgag aacctgctca actacggcaa gcgttactgt gtgtactcgc cgcgcgacgg ccagccgtgc gccgatcacg atcgcgccag cggtgagggt gtgctgccgc aggagtacgc attggtgaag ctcatcgtcc agaacccact cgcgcagccg tgcttcgccg agcacaagga cattatcggc gaccgcaacg tcgtgctgcc cggcgccaca ctccgtcctg agacggtcat cggtcagacg aactgctggg tcagcaacaa gctcaactac aaggggtact acgtgcgcaa cgcgaaggga gaagacgaag tctacatcat gacggcccgc gccgcgcgca atatagcgta ccagaacttc tacgtaaacg gccaggtcgg caccgatccg gagccgctct tcgaggtgga gggagcaagc atggtcggca taccgctttc ggccccgctg gcgccttaca cgacaatcta cacgctgcct atggccacca tcacggaggc gaagggtacc ggcgtcgtca tgtctgtgcc gtccgactcg ccggacgact acatcaactt tacccagctg ctgaacaagc cggaataccg cgtgaagctc ggcatcaaag acgagtgggt ggtgccgttc gcgatgattc cgatcataga cattcccgag ctgggcaccg agggcgccaa gttcatgtgc gagaagctca agatcaacgg cccgaacgcg acggagctgc tagaggaggc gaagaaagtg tgctaccagc agggcttcta ccacggcacc atgatcagtg gcccctacgc cggcgtcaag gtgagcgagg ccaaggtgaa gacgcatcgt gacatggagg cggcggacca gtgcatccgc tactacgagc cggtgcgcca ggtgatcagc cgcagcggtg acgagtgcgt cgtcgcgctg tgcgaccagt ggtacctcga gtatggcaag gacgattgga agaagctggt gcaggaccac atcaagaaga tggacatgtt cttccccggt gtgcgcaacg gcttcgagga gacgctgaac tggctggcgg actggccgtg cagccgcacc ttcggtctcg gcacctacct gccgtgcgac gcgtctcaca cgatgatcat tgacagcctg tcggactcca ccatctacat ggcctactac acgatcgacc gcttcttcaa cgtcggcgcc gacggttcga ccgacctgtg cggcaaggcg gacaacccgt acggtctggc gccagagatg ttcacggacg aggtgttcga gtacatctac cacggcgtcg gcgacgcggc caccgtcgcg ggtgcggtga ggatgccggt cgagtcgctg aagctgatgc gcaacgagtt cgagtactgg taccctgtcg acctgcgctg ctcgggcaag gacctcatcc agaaccacct gacgatgttc ctctacaacc acgccgccat ctggccgacg gacgagagca agtggccgcg ggcaatcttc tgcaacggcc acatccaggt cgataacgaa aagatggcca aatcgagggg gaactttatc tccttgcgtg aggcgatgga catgtacggc gctgacgcca cgcgtcttac ctgcgccgat gccggtgaca gcatggacga cgccaacttc gtgcgcgaga cggcggctgg gtttgtgctg aagatgacga cgctgctgga gcaggcgaag gagacggttg agcgcaccga cctgcgcagc ggcggctaca acgagttcga caagatcttc aataacacaa tgaacacgat catcacccgc accgagggct tctaccaccg catgcagttc cgcatggtgc tcaacacagc cttccacgag ctgtccaacg agtacagtca gtataagatg tactgcgacg acctcaacgt acacgcgcag ctcggccgcc gctactacga ggtggtggcg ctgatgatga tgccactggc gccacacacg atggagcatc tgtggcagaa catcctccag cacgagggct ctgtcgtcac gcagccgttc ccgaagccga cggcgaagct ggactactcg ctgatcgtcg caagccgggt catgacgatc gctctcaagg agattcggtc gcaggtgatc aagaacgcga agaagcgcgg tccggtcgag gaggtcatcg tatacacgcg tcgcgagtac acggactggc agcggcaggc actcgagata ctgcacgagc tctacgaggc gaacggcaac acattcccgg aggatatggc gaagcaagtt gtcgcccgcg acaccaagat catgagcaag gccctgatgg ccttcatgtc cttcgtcaaa ggcaacgtag agaagtacgg ggagcaggcc atgtcgacgc agcccgtcat cgacgatgcc gtgatgttgt cgaacgtcac acacaacctc agccgcctcg ccggcgtatc ggtggtgcgc atcctatccg ccgaagacga gacttacaag gagcacgacg cggcacgccg caagtgcctg cctggtgagc cgtccgtcgc cttgccgcca gtagtgaagc gcgagtag
Details for LeinA.18771.a.B2.GE43059
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 101kDa
OBSERVED MW: 101kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 26
Validated AA Sequence
MFLYNHAAIW PTDESKWPRA IFCNGHIQVD NEKMAKSRGN FISLREAMDM YGADATRLTC ADAGDSMDDA NFVRETAAGF VLKMTTLLEQ AKETVERTDL RSGGYNEFDK IFNNTMNTII TRTEGFYHRM QFRMVLNTAF HELSNEYSQY KMYCDDLNVH AQLGRRYYEV VALMMMPLAP HTMEHLWQNI LQHEGSVVTQ PFPKPTAKLD YSLIVASRVM TIALKEXXSQ VIK
Validated NT Sequence
atcttactca cttacttgat cacctgcgac nnnnnctcct tgagagcgat cgtcatgacc cggcttgcga cgatcagcga gtagtccagc ttcgccgtcg gcttcgggaa cggctgcgtg acgacagagc cctcgtgctg gaggatgttc tgccacagat gctccatcgt gtgtggcgcc agtggcatca tcatcagcgc caccacctcg tagtagcggc ggccgagctg cgcgtgtacg ttgaggtcgt cgcagtacat cttatactga ctgtactcgt tggacagctc gtggaaggct gtgttgagca ccatgcggaa ctgcatgcgg tggtagaagc cctcggtgcg ggtgatgatc gtgttcattg tgttattgaa gatcttgtcg aactcgttgt agccgccgct gcgcaggtcg gtgcgctcaa ccgtctcctt cgcctgctcc agcagcgtcg tcatcttcag cacaaaccca gccgccgtct cgcgcacgaa gttggcgtcg tccatgctgt caccggcatc ggcgcaggta agacgcgtgg cgtcagcgcc gtacatgtcc atcgcctcac gcaaggagat aaagttcccc ctcgatttgg ccatcttttc gttatcgacc tggatgtggc cgttgcagaa gattgcccgc ggccacttgc tctcgtccgt cggccagatg gcggcgtggt tgtagaggaa catcgtcagg tggtnctgga tgnnntnctt gcccgagcag cgcaggtcga cagggtacca gtantcgaan nncgttgcgc atcagcttca gcgactnnac cgnnnnnnnn ncnnnnaccc ncgacnnnnn nnnncgtc
Expected Protein Sequence
MAHHHHHHMN GKLHLGHGFS LTKAEFASRF QRMMGRRSLW PFGFHVTGTP IAACAQKIAK EMQQYGNPPQ FPAELLEDKP KAMVVKEPTE ALGQHKSKRG KSGPAKPQWL IMQSMGIPDS EIAKFADPQY WLDYFPPIAM EDLKSFGCHI DWRRSFMTTE RSPYFDRFVR WQFNILRREN LLNYGKRYCV YSPRDGQPCA DHDRASGEGV LPQEYALVKL IVQNPLAQPC FAEHKDIIGD RNVVLPGATL RPETVIGQTN CWVSNKLNYK GYYVRNAKGE DEVYIMTARA ARNIAYQNFY VNGQVGTDPE PLFEVEGASM VGIPLSAPLA PYTTIYTLPM ATITEAKGTG VVMSVPSDSP DDYINFTQLL NKPEYRVKLG IKDEWVVPFA MIPIIDIPEL GTEGAKFMCE KLKINGPNAT ELLEEAKKVC YQQGFYHGTM ISGPYAGVKV SEAKVKTHRD MEAADQCIRY YEPVRQVISR SGDECVVALC DQWYLEYGKD DWKKLVQDHI KKMDMFFPGV RNGFEETLNW LADWPCSRTF GLGTYLPCDA SHTMIIDSLS DSTIYMAYYT IDRFFNVGAD GSTDLCGKAD NPYGLAPEMF TDEVFEYIYH GVGDAATVAG AVRMPVESLK LMRNEFEYWY PVDLRCSGKD LIQNHLTMFL YNHAAIWPTD ESKWPRAIFC NGHIQVDNEK MAKSRGNFIS LREAMDMYGA DATRLTCADA GDSMDDANFV RETAAGFVLK MTTLLEQAKE TVERTDLRSG GYNEFDKIFN NTMNTIITRT EGFYHRMQFR MVLNTAFHEL SNEYSQYKMY CDDLNVHAQL GRRYYEVVAL MMMPLAPHTM EHLWQNILQH EGSVVTQPFP KPTAKLDYSL IVASRVMTIA LKEIRSQVIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg gcaagcttca cctcggtcat ggcttctcgc tcacgaaggc cgagttcgcc tcgcgcttcc agcgcatgat gggccgccgt tcgctctggc ccttcggctt ccacgtcacc ggcaccccga tcgctgcgtg cgcgcagaaa atcgccaagg agatgcagca gtacggcaac ccgccgcagt tcccggcgga gctgctcgag gacaagccca aggcgatggt ggtcaaggag ccgacggagg cgctagggca gcacaagagc aagcgcggca agagcgggcc ggcgaaaccg cagtggctca tcatgcagag catgggcatc cccgactcgg agatcgcgaa gttcgccgac ccgcagtact ggctggacta cttcccgccc atcgcgatgg aggacttgaa gagttttggc tgccacatcg actggcgccg ctccttcatg acgacggagc gtagcccata ctttgaccgc ttcgtgcggt ggcagttcaa catcctgcgc cgcgagaacc tgctcaacta cggcaagcgt tactgtgtgt actcgccgcg cgacggccag ccgtgcgccg atcacgatcg cgccagcggt gagggtgtgc tgccgcagga gtacgcattg gtgaagctca tcgtccagaa cccactcgcg cagccgtgct tcgccgagca caaggacatt atcggcgacc gcaacgtcgt gctgcccggc gccacactcc gtcctgagac ggtcatcggt cagacgaact gctgggtcag caacaagctc aactacaagg ggtactacgt gcgcaacgcg aagggagaag acgaagtcta catcatgacg gcccgcgccg cgcgcaatat agcgtaccag aacttctacg taaacggcca ggtcggcacc gatccggagc cgctcttcga ggtggaggga gcaagcatgg tcggcatacc gctttcggcc ccgctggcgc cttacacgac aatctacacg ctgcctatgg ccaccatcac ggaggcgaag ggtaccggcg tcgtcatgtc tgtgccgtcc gactcgccgg acgactacat caactttacc cagctgctga acaagccgga ataccgcgtg aagctcggca tcaaagacga gtgggtggtg ccgttcgcga tgattccgat catagacatt cccgagctgg gcaccgaggg cgccaagttc atgtgcgaga agctcaagat caacggcccg aacgcgacgg agctgctaga ggaggcgaag aaagtgtgct accagcaggg cttctaccac ggcaccatga tcagtggccc ctacgccggc gtcaaggtga gcgaggccaa ggtgaagacg catcgtgaca tggaggcggc ggaccagtgc atccgctact acgagccggt gcgccaggtg atcagccgca gcggtgacga gtgcgtcgtc gcgctgtgcg accagtggta cctcgagtat ggcaaggacg attggaagaa gctggtgcag gaccacatca agaagatgga catgttcttc cccggtgtgc gcaacggctt cgaggagacg ctgaactggc tggcggactg gccgtgcagc cgcaccttcg gtctcggcac ctacctgccg tgcgacgcgt ctcacacgat gatcattgac agcctgtcgg actccaccat ctacatggcc tactacacga tcgaccgctt cttcaacgtc ggcgccgacg gttcgaccga cctgtgcggc aaggcggaca acccgtacgg tctggcgcca gagatgttca cggacgaggt gttcgagtac atctaccacg gcgtcggcga cgcggccacc gtcgcgggtg cggtgaggat gccggtcgag tcgctgaagc tgatgcgcaa cgagttcgag tactggtacc ctgtcgacct gcgctgctcg ggcaaggacc tcatccagaa ccacctgacg atgttcctct acaaccacgc cgccatctgg ccgacggacg agagcaagtg gccgcgggca atcttctgca acggccacat ccaggtcgat aacgaaaaga tggccaaatc gagggggaac tttatctcct tgcgtgaggc gatggacatg tacggcgctg acgccacgcg tcttacctgc gccgatgccg gtgacagcat ggacgacgcc aacttcgtgc gcgagacggc ggctgggttt gtgctgaaga tgacgacgct gctggagcag gcgaaggaga cggttgagcg caccgacctg cgcagcggcg gctacaacga gttcgacaag atcttcaata acacaatgaa cacgatcatc acccgcaccg agggcttcta ccaccgcatg cagttccgca tggtgctcaa cacagccttc cacgagctgt ccaacgagta cagtcagtat aagatgtact gcgacgacct caacgtacac gcgcagctcg gccgccgcta ctacgaggtg gtggcgctga tgatgatgcc actggcgcca cacacgatgg agcatctgtg gcagaacatc ctccagcacg agggctctgt cgtcacgcag ccgttcccga agccgacggc gaagctggac tactcgctga tcgtcgcaag ccgggtcatg acgatcgctc tcaaggagat tcggtcgcag gtgatcaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg 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