LeinA.18749.a
Uncharacterized protein
CENTER ID:
LeinA.18749.a
ORGANISM:
Leishmania infantum
ASSOCIATED DISEASE:
Visceral leishmaniasis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
unclassified
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
LeinA.18749.a.B1.GE37560
full length
info
1
730
LeinA.18749.a.B1.GE37560
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
LeinA.18749.a.B1.PS02018
full length
info
1
730
unavailable
Structures
4TYZ
DEPOSITED: 7/9/2014
DETERMINATION: XRay
CLONE: LeinA.18749.a.B1.GE37560
PROTEIN: LeinA.18749.a.B1.PS02018
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKNRTPAE IQRSFVQHVQ NISAHNRGIS DELALMSQGL RDGIQSNTGP TTDLLAAASE LSRFAMRACT NLSSCLEDLV NMGEAMAPVL AANPEAAEPL LLNFFSTFVT QMGAFYNEID AWWGKGCESL GRGKAAFFRE SSVSNTALAT REPTASTAPT GAMPTADRER EVSETAAVST VHAGRRRNHQ LLEDDVADGE PPPPHDCYSE SAPGGAAPKK GRKKKATTAA EAEASATAAT TPTTKRTQNF DPLPNSQSSR GSAEDTMAGL AAAAESGAQT ALPQRPEKSS SRSVLPVADE LAEMTPEDLN ALAQSEPSYL FLTSTLEDAL GAVVMASRPN VFSNLASFTY NEPVSTYYIR LIRPGSLAYF VYCNYILGSA DTQADFLCDV LETDCAEVDP FTSPAGEVIV EDAADLSSLP ACAWAIGSSE DNERRLYTMY ALIEQPPRVG PTAIIQDSLN MGAARDEVLH MVTLVDGAPE FARVTCMVYS EMRTAEIPTQ KQAAILSFSR SGFAHTGFVE VPDSEVEAAV SRMRRKKQNA AGYKTVLFSK QELDATTTFQ PPVQLASLNQ PAGDAGISLG GVARGAVQGG FEVLKAPFTV GRAVGGALLD ATGVTGAVRN NIEGVFRKSF PDLAGETLLD SFNCAWVEGS ALKQGYLFIT PHWLCFQSTL AAAHFSIEYD EIKDIIKSKS VKMFENAIEV KTHLNDTIFL TNFLQRDQAY SALMSQWLKQ
NT Sequence
atgagcgcga aaaaccgcac tccagctgag attcagagga gtttcgtgca gcatgtgcag aacatttccg cccacaaccg tggcatcagc gacgagctgg ccctcatgtc acagggcctg cgagatggca tacaaagcaa caccggcccc acgacggatc tgctcgctgc cgcaagtgag ctgagccgct tcgcgatgag ggcgtgcacg aacctgtcca gctgtctgga ggatctggtc aacatgggcg aggctatggc acctgtgctg gccgccaatc cggaggcggc ggagccactg ctgttgaact ttttcagcac cttcgtgaca cagatgggcg cattctacaa tgagattgat gcatggtggg gcaagggctg cgagagcttg ggccgcggca aggcagcgtt cttcagagaa tcgtcagtct ctaacaccgc actggcgacc agggagccaa ccgcttccac ggccccgacg ggcgcgatgc cgacagcaga cagagagcgt gaggtgtccg aaacggcggc cgtatcgact gtgcatgcag ggcgccgcag gaatcaccag ctgctcgagg atgacgtagc ggacggagag ccaccgccgc cgcacgactg ttacagcgaa tcagcgcctg gcggtgccgc tcccaagaag ggacgaaaga agaaggctac cactgccgcc gaagcagagg catccgcgac ggcggcgacg acgccgacca cgaagcggac acagaacttt gatccgttgc cgaatagtca gtctagccgc ggcagtgccg aagacacgat ggccggcttg gcggcggcgg cggagtcagg tgctcagaca gccttgccac aacggcctga gaagagcagc tcacgctccg ttctccccgt cgccgacgaa ctggcggaga tgacgccgga ggacctgaat gccttggcgc agtctgaacc atcttacctg ttcctcacct ccaccctgga ggatgcactt ggggcggtcg tcatggcctc ccgccccaac gtctttagta acctggccag cttcacctat aatgagcccg tttccacgta ttacatccgc cttatccgcc ccggcagctt ggcgtacttt gtctactgca actacattct cggcagcgcg gacacgcagg ccgacttcct gtgtgacgtg ctggagaccg actgcgctga ggtggaccca ttcacgtctc ccgccggcga ggtgatcgtt gaagacgcag cggatctctc cagcctgccg gcgtgcgcgt gggctatcgg cagcagcgag gacaacgagc gccgcctgta cacaatgtac gcgctcatcg agcagccgcc gcgagtcggc ccgaccgcca tcatacaaga ctcgctgaat atgggtgctg cgcgggatga ggttctgcac atggtgacgc tggtggacgg cgcacccgag tttgcgcgag tgacgtgcat ggtgtactct gagatgcgca ctgcagagat accgacccag aagcaagcgg ccatcctcag cttctcgcgc agcgggttcg cgcataccgg ctttgtcgag gtccccgact cggaagtcga ggcggcggtg tcacgcatga ggcgcaaaaa acagaatgcg gcgggctata agacggtgct cttttcgaag caggagctgg atgccacgac gacgtttcaa ccaccagtcc agctcgcctc gctcaatcag cccgctggcg acgcaggaat atcacttggc ggtgttgcgc gtggggccgt gcaaggcggc ttcgaagtgc tgaaggcgcc cttcacggtg ggccgcgcag tcggcggcgc cctgctcgac gccactggtg tcaccggcgc ggtgcgcaac aacatcgagg gcgtctttcg caagtcgttt ccggacttag ctggcgagac gctcttggat tccttcaact gcgcctgggt cgaagggtcc gcgctgaagc aaggctactt gttcatcacg ccgcactggc tgtgctttca gagcaccttg gccgccgcgc acttttccat cgaatacgac gagatcaaag atattatcaa gtcaaagtcg gtgaagatgt ttgagaatgc gatcgaggtg aagacgcacc tgaatgacac gatcttcctc acaaactttc tgcagcggga tcaagcgtat agcgctctga tgagtcagtg gctgaagcag taa
Details for LeinA.18749.a.B1.GE37560
HARVESTED ON:
11/25/2013
SEQUENCED ON:
6/9/2016
EXPECTED MW:
79kDa
OBSERVED MW:
79kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
98
PERCENT COVERAGE:
44
Details for LeinA.18749.a.B1.PS02018
PURIFICATION DATe:
3/13/2014
CONCENTRATION:
8.28mg/ml
OBSERVED MW:
82kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
data unavailable
VIAL VOLUME:
110µl
PERCENT IDENTITY:
98
PERCENT COVERAGE:
44