LeinA.18749.a
Uncharacterized protein
| CENTER ID: | LeinA.18749.a |
| ORGANISM: | Leishmania infantum |
| ASSOCIATED DISEASE: | Visceral leishmaniasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LeinA.18749.a.B1.GE37560 | full length | 1 | 730 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LeinA.18749.a.B1.PS02018 | full length | 1 | 730 | unavailable |
Structures
4TYZ
DEPOSITED: 7/9/2014
DETERMINATION: XRay
CLONE: LeinA.18749.a.B1.GE37560
PROTEIN: LeinA.18749.a.B1.PS02018
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:LinJ.30.2720 |
| RefSeq: | XP_001467118.1 |
| UniProt: | A4I5U9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKNRTPAE IQRSFVQHVQ NISAHNRGIS DELALMSQGL RDGIQSNTGP TTDLLAAASE LSRFAMRACT NLSSCLEDLV NMGEAMAPVL AANPEAAEPL LLNFFSTFVT QMGAFYNEID AWWGKGCESL GRGKAAFFRE SSVSNTALAT REPTASTAPT GAMPTADRER EVSETAAVST VHAGRRRNHQ LLEDDVADGE PPPPHDCYSE SAPGGAAPKK GRKKKATTAA EAEASATAAT TPTTKRTQNF DPLPNSQSSR GSAEDTMAGL AAAAESGAQT ALPQRPEKSS SRSVLPVADE LAEMTPEDLN ALAQSEPSYL FLTSTLEDAL GAVVMASRPN VFSNLASFTY NEPVSTYYIR LIRPGSLAYF VYCNYILGSA DTQADFLCDV LETDCAEVDP FTSPAGEVIV EDAADLSSLP ACAWAIGSSE DNERRLYTMY ALIEQPPRVG PTAIIQDSLN MGAARDEVLH MVTLVDGAPE FARVTCMVYS EMRTAEIPTQ KQAAILSFSR SGFAHTGFVE VPDSEVEAAV SRMRRKKQNA AGYKTVLFSK QELDATTTFQ PPVQLASLNQ PAGDAGISLG GVARGAVQGG FEVLKAPFTV GRAVGGALLD ATGVTGAVRN NIEGVFRKSF PDLAGETLLD SFNCAWVEGS ALKQGYLFIT PHWLCFQSTL AAAHFSIEYD EIKDIIKSKS VKMFENAIEV KTHLNDTIFL TNFLQRDQAY SALMSQWLKQ
NT Sequence
atgagcgcga aaaaccgcac tccagctgag attcagagga gtttcgtgca gcatgtgcag aacatttccg cccacaaccg tggcatcagc gacgagctgg ccctcatgtc acagggcctg cgagatggca tacaaagcaa caccggcccc acgacggatc tgctcgctgc cgcaagtgag ctgagccgct tcgcgatgag ggcgtgcacg aacctgtcca gctgtctgga ggatctggtc aacatgggcg aggctatggc acctgtgctg gccgccaatc cggaggcggc ggagccactg ctgttgaact ttttcagcac cttcgtgaca cagatgggcg cattctacaa tgagattgat gcatggtggg gcaagggctg cgagagcttg ggccgcggca aggcagcgtt cttcagagaa tcgtcagtct ctaacaccgc actggcgacc agggagccaa ccgcttccac ggccccgacg ggcgcgatgc cgacagcaga cagagagcgt gaggtgtccg aaacggcggc cgtatcgact gtgcatgcag ggcgccgcag gaatcaccag ctgctcgagg atgacgtagc ggacggagag ccaccgccgc cgcacgactg ttacagcgaa tcagcgcctg gcggtgccgc tcccaagaag ggacgaaaga agaaggctac cactgccgcc gaagcagagg catccgcgac ggcggcgacg acgccgacca cgaagcggac acagaacttt gatccgttgc cgaatagtca gtctagccgc ggcagtgccg aagacacgat ggccggcttg gcggcggcgg cggagtcagg tgctcagaca gccttgccac aacggcctga gaagagcagc tcacgctccg ttctccccgt cgccgacgaa ctggcggaga tgacgccgga ggacctgaat gccttggcgc agtctgaacc atcttacctg ttcctcacct ccaccctgga ggatgcactt ggggcggtcg tcatggcctc ccgccccaac gtctttagta acctggccag cttcacctat aatgagcccg tttccacgta ttacatccgc cttatccgcc ccggcagctt ggcgtacttt gtctactgca actacattct cggcagcgcg gacacgcagg ccgacttcct gtgtgacgtg ctggagaccg actgcgctga ggtggaccca ttcacgtctc ccgccggcga ggtgatcgtt gaagacgcag cggatctctc cagcctgccg gcgtgcgcgt gggctatcgg cagcagcgag gacaacgagc gccgcctgta cacaatgtac gcgctcatcg agcagccgcc gcgagtcggc ccgaccgcca tcatacaaga ctcgctgaat atgggtgctg cgcgggatga ggttctgcac atggtgacgc tggtggacgg cgcacccgag tttgcgcgag tgacgtgcat ggtgtactct gagatgcgca ctgcagagat accgacccag aagcaagcgg ccatcctcag cttctcgcgc agcgggttcg cgcataccgg ctttgtcgag gtccccgact cggaagtcga ggcggcggtg tcacgcatga ggcgcaaaaa acagaatgcg gcgggctata agacggtgct cttttcgaag caggagctgg atgccacgac gacgtttcaa ccaccagtcc agctcgcctc gctcaatcag cccgctggcg acgcaggaat atcacttggc ggtgttgcgc gtggggccgt gcaaggcggc ttcgaagtgc tgaaggcgcc cttcacggtg ggccgcgcag tcggcggcgc cctgctcgac gccactggtg tcaccggcgc ggtgcgcaac aacatcgagg gcgtctttcg caagtcgttt ccggacttag ctggcgagac gctcttggat tccttcaact gcgcctgggt cgaagggtcc gcgctgaagc aaggctactt gttcatcacg ccgcactggc tgtgctttca gagcaccttg gccgccgcgc acttttccat cgaatacgac gagatcaaag atattatcaa gtcaaagtcg gtgaagatgt ttgagaatgc gatcgaggtg aagacgcacc tgaatgacac gatcttcctc acaaactttc tgcagcggga tcaagcgtat agcgctctga tgagtcagtg gctgaagcag taa
Details for LeinA.18749.a.B1.GE37560
| HARVESTED ON: | 11/25/2013 |
| SEQUENCED ON: | 6/9/2016 |
| EXPECTED MW: | 79kDa |
| OBSERVED MW: | 79kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 44 |
Validated AA Sequence
MAHHHHHHMS AKNRTPAEIQ RSFVQHVQNI SAHNRGISDE LALMSQGLRD GIQSNTGPTT DLLAAASELS RFAMRACTNL SSCLEDLVNM GEAMAPVLAA NPEAAEPLLL NFFSTFVTQM GAFYNEIDAW WGKGCESLGR GKAAFFRESS VSNTALATRE PTASTAPTGA MPTADREREV SETAAVSTVH AGRRRNHQLL EDDVADGEPP PPHDCYSEST PGGAAPKKGR KKKATTAAEA EASATAATTP TTKRTQNFDP LPNSQSSRGS AEDTMAGLAA AAESGAQTAL PQRPEKSSSR SVLPVADELA ELTPXDLNAL AQSXXSYL
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gcgaaaaacc gcactccagc tgagattcag aggagtttcg tgcagcatgt gcagaacatt tccgcccaca accgtggcat cagcgacgag ctggccctca tgtcacaggg cctgcgagat ggcatacaaa gcaacaccgg ccccacgacg gatctgctcg ctgccgcaag tgagctgagc cgcttcgcga tgagggcgtg cacgaacctg tccagctgtc tggaggatct ggtcaacatg ggcgaggcta tggcacctgt gctggccgcc aatccggagg cggcggagcc actgctgttg aactttttca gcaccttcgt gacacagatg ggcgcattct acaatgagat tgatgcatgg tggggcaagg gctgcgagag cttgggccgc ggcaaggcag cgttcttcag agaatcgtca gtctctaaca ccgcactggc gaccagggag ccaaccgcct ccacggcccc gacgggcgcg atgccgacag cagacagaga gcgtgaggtg tccgaaacgg cggccgtatc gactgtgcat gcagggcgcc gcaggaatca ccagctgctc gaggatgacg tagcggacgg agagccaccg ccgccgcacg actgttacag cgaatcaacg cctggcggtg ccgctcccaa gaagggacga aagaagaagg ctaccactgc cgccgaagca gaggcatccg cgacggcggc gacgacgccg accacgaagc ggacacagaa ctttgatccg ttgccgaata gtcagtctag ccgcggcagt gccgaagaca cgatggccgg cttggcggcg gcggcggagt caggtgctca gacagccttg ccacaacggc ctgagaagag cagctcacgc tccgttctcc ccgtcgccga cgaactggcg gagctgacgc cnnaggacct gaatgccttg gcgcagtctn nnnnntcnta cctgt
Expected Protein Sequence
MAHHHHHHMS AKNRTPAEIQ RSFVQHVQNI SAHNRGISDE LALMSQGLRD GIQSNTGPTT DLLAAASELS RFAMRACTNL SSCLEDLVNM GEAMAPVLAA NPEAAEPLLL NFFSTFVTQM GAFYNEIDAW WGKGCESLGR GKAAFFRESS VSNTALATRE PTASTAPTGA MPTADREREV SETAAVSTVH AGRRRNHQLL EDDVADGEPP PPHDCYSESA PGGAAPKKGR KKKATTAAEA EASATAATTP TTKRTQNFDP LPNSQSSRGS AEDTMAGLAA AAESGAQTAL PQRPEKSSSR SVLPVADELA EMTPEDLNAL AQSEPSYLFL TSTLEDALGA VVMASRPNVF SNLASFTYNE PVSTYYIRLI RPGSLAYFVY CNYILGSADT QADFLCDVLE TDCAEVDPFT SPAGEVIVED AADLSSLPAC AWAIGSSEDN ERRLYTMYAL IEQPPRVGPT AIIQDSLNMG AARDEVLHMV TLVDGAPEFA RVTCMVYSEM RTAEIPTQKQ AAILSFSRSG FAHTGFVEVP DSEVEAAVSR MRRKKQNAAG YKTVLFSKQE LDATTTFQPP VQLASLNQPA GDAGISLGGV ARGAVQGGFE VLKAPFTVGR AVGGALLDAT GVTGAVRNNI EGVFRKSFPD LAGETLLDSF NCAWVEGSAL KQGYLFITPH WLCFQSTLAA AHFSIEYDEI KDIIKSKSVK MFENAIEVKT HLNDTIFLTN FLQRDQAYSA LMSQWLKQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg cgaaaaaccg cactccagct gagattcaga ggagtttcgt gcagcatgtg cagaacattt ccgcccacaa ccgtggcatc agcgacgagc tggccctcat gtcacagggc ctgcgagatg gcatacaaag caacaccggc cccacgacgg atctgctcgc tgccgcaagt gagctgagcc gcttcgcgat gagggcgtgc acgaacctgt ccagctgtct ggaggatctg gtcaacatgg gcgaggctat ggcacctgtg ctggccgcca atccggaggc ggcggagcca ctgctgttga actttttcag caccttcgtg acacagatgg gcgcattcta caatgagatt gatgcatggt ggggcaaggg ctgcgagagc ttgggccgcg gcaaggcagc gttcttcaga gaatcgtcag tctctaacac cgcactggcg accagggagc caaccgcttc cacggccccg acgggcgcga tgccgacagc agacagagag cgtgaggtgt ccgaaacggc ggccgtatcg actgtgcatg cagggcgccg caggaatcac cagctgctcg aggatgacgt agcggacgga gagccaccgc cgccgcacga ctgttacagc gaatcagcgc ctggcggtgc cgctcccaag aagggacgaa agaagaaggc taccactgcc gccgaagcag aggcatccgc gacggcggcg acgacgccga ccacgaagcg gacacagaac tttgatccgt tgccgaatag tcagtctagc cgcggcagtg ccgaagacac gatggccggc ttggcggcgg cggcggagtc aggtgctcag acagccttgc cacaacggcc tgagaagagc agctcacgct ccgttctccc cgtcgccgac gaactggcgg agatgacgcc ggaggacctg aatgccttgg cgcagtctga accatcttac ctgttcctca cctccaccct ggaggatgca cttggggcgg tcgtcatggc ctcccgcccc aacgtcttta gtaacctggc cagcttcacc tataatgagc ccgtttccac gtattacatc cgccttatcc gccccggcag cttggcgtac tttgtctact gcaactacat tctcggcagc gcggacacgc aggccgactt cctgtgtgac gtgctggaga ccgactgcgc tgaggtggac ccattcacgt ctcccgccgg cgaggtgatc gttgaagacg cagcggatct ctccagcctg ccggcgtgcg cgtgggctat cggcagcagc gaggacaacg agcgccgcct gtacacaatg tacgcgctca tcgagcagcc gccgcgagtc ggcccgaccg ccatcataca agactcgctg aatatgggtg ctgcgcggga tgaggttctg cacatggtga cgctggtgga cggcgcaccc gagtttgcgc gagtgacgtg catggtgtac tctgagatgc gcactgcaga gataccgacc cagaagcaag cggccatcct cagcttctcg cgcagcgggt tcgcgcatac cggctttgtc gaggtccccg actcggaagt cgaggcggcg gtgtcacgca tgaggcgcaa aaaacagaat gcggcgggct ataagacggt gctcttttcg aagcaggagc tggatgccac gacgacgttt caaccaccag tccagctcgc ctcgctcaat cagcccgctg gcgacgcagg aatatcactt ggcggtgttg cgcgtggggc cgtgcaaggc ggcttcgaag tgctgaaggc gcccttcacg gtgggccgcg cagtcggcgg cgccctgctc gacgccactg gtgtcaccgg cgcggtgcgc aacaacatcg agggcgtctt tcgcaagtcg tttccggact tagctggcga gacgctcttg gattccttca actgcgcctg ggtcgaaggg tccgcgctga agcaaggcta cttgttcatc acgccgcact ggctgtgctt tcagagcacc ttggccgccg cgcacttttc catcgaatac gacgagatca aagatattat caagtcaaag tcggtgaaga tgtttgagaa tgcgatcgag gtgaagacgc acctgaatga cacgatcttc ctcacaaact ttctgcagcg ggatcaagcg tatagcgctc tgatgagtca gtggctgaag cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LeinA.18749.a.B1.PS02018
| PURIFICATION DATe: | 3/13/2014 |
| CONCENTRATION: | 8.28mg/ml |
| OBSERVED MW: | 82kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 44 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS AKNRTPAEIQ RSFVQHVQNI SAHNRGISDE LALMSQGLRD GIQSNTGPTT DLLAAASELS RFAMRACTNL SSCLEDLVNM GEAMAPVLAA NPEAAEPLLL NFFSTFVTQM GAFYNEIDAW WGKGCESLGR GKAAFFRESS VSNTALATRE PTASTAPTGA MPTADREREV SETAAVSTVH AGRRRNHQLL EDDVADGEPP PPHDCYSEST PGGAAPKKGR KKKATTAAEA EASATAATTP TTKRTQNFDP LPNSQSSRGS AEDTMAGLAA AAESGAQTAL PQRPEKSSSR SVLPVADELA ELTPXDLNAL AQSXXSYL
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gcgaaaaacc gcactccagc tgagattcag aggagtttcg tgcagcatgt gcagaacatt tccgcccaca accgtggcat cagcgacgag ctggccctca tgtcacaggg cctgcgagat ggcatacaaa gcaacaccgg ccccacgacg gatctgctcg ctgccgcaag tgagctgagc cgcttcgcga tgagggcgtg cacgaacctg tccagctgtc tggaggatct ggtcaacatg ggcgaggcta tggcacctgt gctggccgcc aatccggagg cggcggagcc actgctgttg aactttttca gcaccttcgt gacacagatg ggcgcattct acaatgagat tgatgcatgg tggggcaagg gctgcgagag cttgggccgc ggcaaggcag cgttcttcag agaatcgtca gtctctaaca ccgcactggc gaccagggag ccaaccgcct ccacggcccc gacgggcgcg atgccgacag cagacagaga gcgtgaggtg tccgaaacgg cggccgtatc gactgtgcat gcagggcgcc gcaggaatca ccagctgctc gaggatgacg tagcggacgg agagccaccg ccgccgcacg actgttacag cgaatcaacg cctggcggtg ccgctcccaa gaagggacga aagaagaagg ctaccactgc cgccgaagca gaggcatccg cgacggcggc gacgacgccg accacgaagc ggacacagaa ctttgatccg ttgccgaata gtcagtctag ccgcggcagt gccgaagaca cgatggccgg cttggcggcg gcggcggagt caggtgctca gacagccttg ccacaacggc ctgagaagag cagctcacgc tccgttctcc ccgtcgccga cgaactggcg gagctgacgc cnnaggacct gaatgccttg gcgcagtctn nnnnntcnta cctgt
Expressed Protein Sequence
MAHHHHHHMS AKNRTPAEIQ RSFVQHVQNI SAHNRGISDE LALMSQGLRD GIQSNTGPTT DLLAAASELS RFAMRACTNL SSCLEDLVNM GEAMAPVLAA NPEAAEPLLL NFFSTFVTQM GAFYNEIDAW WGKGCESLGR GKAAFFRESS VSNTALATRE PTASTAPTGA MPTADREREV SETAAVSTVH AGRRRNHQLL EDDVADGEPP PPHDCYSESA PGGAAPKKGR KKKATTAAEA EASATAATTP TTKRTQNFDP LPNSQSSRGS AEDTMAGLAA AAESGAQTAL PQRPEKSSSR SVLPVADELA EMTPEDLNAL AQSEPSYLFL TSTLEDALGA VVMASRPNVF SNLASFTYNE PVSTYYIRLI RPGSLAYFVY CNYILGSADT QADFLCDVLE TDCAEVDPFT SPAGEVIVED AADLSSLPAC AWAIGSSEDN ERRLYTMYAL IEQPPRVGPT AIIQDSLNMG AARDEVLHMV TLVDGAPEFA RVTCMVYSEM RTAEIPTQKQ AAILSFSRSG FAHTGFVEVP DSEVEAAVSR MRRKKQNAAG YKTVLFSKQE LDATTTFQPP VQLASLNQPA GDAGISLGGV ARGAVQGGFE VLKAPFTVGR AVGGALLDAT GVTGAVRNNI EGVFRKSFPD LAGETLLDSF NCAWVEGSAL KQGYLFITPH WLCFQSTLAA AHFSIEYDEI KDIIKSKSVK MFENAIEVKT HLNDTIFLTN FLQRDQAYSA LMSQWLKQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CGAAAAACCG CACTCCAGCT GAGATTCAGA GGAGTTTCGT GCAGCATGTG CAGAACATTT CCGCCCACAA CCGTGGCATC AGCGACGAGC TGGCCCTCAT GTCACAGGGC CTGCGAGATG GCATACAAAG CAACACCGGC CCCACGACGG ATCTGCTCGC TGCCGCAAGT GAGCTGAGCC GCTTCGCGAT GAGGGCGTGC ACGAACCTGT CCAGCTGTCT GGAGGATCTG GTCAACATGG GCGAGGCTAT GGCACCTGTG CTGGCCGCCA ATCCGGAGGC GGCGGAGCCA CTGCTGTTGA ACTTTTTCAG CACCTTCGTG ACACAGATGG GCGCATTCTA CAATGAGATT GATGCATGGT GGGGCAAGGG CTGCGAGAGC TTGGGCCGCG GCAAGGCAGC GTTCTTCAGA GAATCGTCAG TCTCTAACAC CGCACTGGCG ACCAGGGAGC CAACCGCTTC CACGGCCCCG ACGGGCGCGA TGCCGACAGC AGACAGAGAG CGTGAGGTGT CCGAAACGGC GGCCGTATCG ACTGTGCATG CAGGGCGCCG CAGGAATCAC CAGCTGCTCG AGGATGACGT AGCGGACGGA GAGCCACCGC CGCCGCACGA CTGTTACAGC GAATCAGCGC CTGGCGGTGC CGCTCCCAAG AAGGGACGAA AGAAGAAGGC TACCACTGCC GCCGAAGCAG AGGCATCCGC GACGGCGGCG ACGACGCCGA CCACGAAGCG GACACAGAAC TTTGATCCGT TGCCGAATAG TCAGTCTAGC CGCGGCAGTG CCGAAGACAC GATGGCCGGC TTGGCGGCGG CGGCGGAGTC AGGTGCTCAG ACAGCCTTGC CACAACGGCC TGAGAAGAGC AGCTCACGCT CCGTTCTCCC CGTCGCCGAC GAACTGGCGG AGATGACGCC GGAGGACCTG AATGCCTTGG CGCAGTCTGA ACCATCTTAC CTGTTCCTCA CCTCCACCCT GGAGGATGCA CTTGGGGCGG TCGTCATGGC CTCCCGCCCC AACGTCTTTA GTAACCTGGC CAGCTTCACC TATAATGAGC CCGTTTCCAC GTATTACATC CGCCTTATCC GCCCCGGCAG CTTGGCGTAC TTTGTCTACT GCAACTACAT TCTCGGCAGC GCGGACACGC AGGCCGACTT CCTGTGTGAC GTGCTGGAGA CCGACTGCGC TGAGGTGGAC CCATTCACGT CTCCCGCCGG CGAGGTGATC GTTGAAGACG CAGCGGATCT CTCCAGCCTG CCGGCGTGCG CGTGGGCTAT CGGCAGCAGC GAGGACAACG AGCGCCGCCT GTACACAATG TACGCGCTCA TCGAGCAGCC GCCGCGAGTC GGCCCGACCG CCATCATACA AGACTCGCTG AATATGGGTG CTGCGCGGGA TGAGGTTCTG CACATGGTGA CGCTGGTGGA CGGCGCACCC GAGTTTGCGC GAGTGACGTG CATGGTGTAC TCTGAGATGC GCACTGCAGA GATACCGACC CAGAAGCAAG CGGCCATCCT CAGCTTCTCG CGCAGCGGGT TCGCGCATAC CGGCTTTGTC GAGGTCCCCG ACTCGGAAGT CGAGGCGGCG GTGTCACGCA TGAGGCGCAA AAAACAGAAT GCGGCGGGCT ATAAGACGGT GCTCTTTTCG AAGCAGGAGC TGGATGCCAC GACGACGTTT CAACCACCAG TCCAGCTCGC CTCGCTCAAT CAGCCCGCTG GCGACGCAGG AATATCACTT GGCGGTGTTG CGCGTGGGGC CGTGCAAGGC GGCTTCGAAG TGCTGAAGGC GCCCTTCACG GTGGGCCGCG CAGTCGGCGG CGCCCTGCTC GACGCCACTG GTGTCACCGG CGCGGTGCGC AACAACATCG AGGGCGTCTT TCGCAAGTCG TTTCCGGACT TAGCTGGCGA GACGCTCTTG GATTCCTTCA ACTGCGCCTG GGTCGAAGGG TCCGCGCTGA AGCAAGGCTA CTTGTTCATC ACGCCGCACT GGCTGTGCTT TCAGAGCACC TTGGCCGCCG CGCACTTTTC CATCGAATAC GACGAGATCA AAGATATTAT CAAGTCAAAG TCGGTGAAGA TGTTTGAGAA TGCGATCGAG GTGAAGACGC ACCTGAATGA CACGATCTTC CTCACAAACT TTCTGCAGCG GGATCAAGCG TATAGCGCTC TGATGAGTCA GTGGCTGAAG CAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT