SSGCID: Target Page

LeinA.18746.a

Uncharacterized protein

CENTER ID:	LeinA.18746.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18746.a.B1.GE37559	full length		1	237

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.31.2870
RefSeq:	XP_001467602.1
UniProt:	A4I783

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLSQTGGAVA VNEISQQHST EQAMAAKEMA AENQVHVTLP EEVRERRRSR QSSQARNGSH EAPIISYGEA APKQTVSRVL VRMPNEVRRR RSLSREARKS HTHTQLEESE MPNATPKAMP EAAAMSTAED GRVSPEKQCP EPELPANGLA MQPAVEEAVD HEPPTEPSPA AEEAERSGRL TDAASRVEME LTGSDSAAVI EEANQEMEDN AAPHENNDAH PMIKGKEKDK HGCCIVM

NT Sequence

atgctcagcc agaccggagg ggcagtcgcc gtcaacgaga tctcgcagca gcactcgacc gagcaagcga tggctgccaa agaaatggca gcggagaacc aggtgcacgt gacgctgccc gaggaggtga gggagcgccg ccgctctcgt caatcgtcgc aggcgcgtaa cgggtcccac gaggcaccga tcatttccta cggcgaggcg gcgccgaagc agacggtctc gcgcgtgttg gtgcggatgc cgaatgaggt acgccgcagg cgaagcctct ctcgcgaggc ccgtaagagc catacccaca cgcagctgga ggagagcgag atgccgaacg ccacccctaa agccatgccc gaagccgccg ctatgtcgac tgccgaagat ggtcgtgtta gcccggagaa gcagtgtcca gagccggagc tgcccgccaa cggcttggcc atgcagcccg ctgtcgagga ggcagtcgat catgagccgc cgacagaacc ctcacccgcg gccgaagagg ccgagagatc cgggaggctc accgacgcgg cctctcgggt ggaaatggag ttgacaggga gcgactccgc tgctgtcatc gaggaggcta accaggaaat ggaggataat gcggcgcctc acgagaacaa tgatgcgcac ccgatgatca agggcaagga gaaggataag cacggatgct gcatagtgat gtaa

Details for LeinA.18746.a.B1.GE37559

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/4/2013
EXPECTED MW:	27kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML SQTGGAVAVN EISQQHSTEQ AMAAKEMAAE NQVHVTLPEE VRERRRSRQS SQARNGSHEA LIISYGEAAP KQTVSRVLVR MPNEVRRRRS LSREARKSHT HTQLEESEMP NATPKAMPEA AAMSTAEDGR VSPEKQCPEP ELPANGLAMQ PAVEEAVDHE PPTEPSPAAE EAERSGRLTD AASRVEMELT GRDSAAVIEE ANQEMEDNAA PHENNDAHPM IKGKEKDKHG CCIVM

Validated NT Sequence

cnnnnnnnna ntattttgtt tactttaaga aggagatata ccatggctca ccaccaccac caccatatgc tcagccagac cggaggggca gtcgccgtca acgagatctc gcagcagcac tcgaccgagc aagcgatggc tgccaaagaa atggcagcgg agaaccaggt gcacgtgacg ctgcccgagg aggtgaggga gcgccgccgc tctcgtcaat cgtcgcaggc gcgtaacggg tcccacgagg cactgatcat ttcctacggt gaggcggcgc cgaagcagac ggtctcgcgc gtgttggtgc ggatgccgaa tgaggtacgc cgcaggcgaa gcctctctcg cgaggcccgc aagagccata cccacacgca gctggaggag agcgagatgc cgaacgccac ccctaaagcc atgcccgaag ccgccgctat gtcgactgcc gaagatggtc gtgttagccc ggagaagcag tgtccagagc cggagctgcc cgccaacggc ttggccatgc agcccgctgt cgaggaggca gtcgatcatg agccgccgac agaaccctca cccgcggccg aagaggccga gagatccggg aggctcaccg acgcggcctc tcgggtggaa atggagttga cagggcgaga ctccgctgct gtcatcgagg aggctaacca ggaaatggag gataatgcgg cgcctcacga gaacaatgat gcgcacccga tgatcaaggg caaggagaag gataagcacg gatgctgcat agtgatgtaa gtgagtaaga taggatccgg ctgtnnnnnn cccnnnnaaa nananaannn aannnnnnna aaaaaanggt nncaggatag tatcggtgta ncgcnnnccc atcccgcatg tanaattagg tctacttgaa ataatcttcg tnnccntcnt ttgagcccgg ggcctannac tttattccaa actctngagc gggannntga tnnntnnnnn naaatcattt ttctcgtaga tgaaggcgnc nnaaacgggg ngcggnaggg ntttctacgn naggntnnan cc

Expected Protein Sequence

MAHHHHHHML SQTGGAVAVN EISQQHSTEQ AMAAKEMAAE NQVHVTLPEE VRERRRSRQS SQARNGSHEA PIISYGEAAP KQTVSRVLVR MPNEVRRRRS LSREARKSHT HTQLEESEMP NATPKAMPEA AAMSTAEDGR VSPEKQCPEP ELPANGLAMQ PAVEEAVDHE PPTEPSPAAE EAERSGRLTD AASRVEMELT GSDSAAVIEE ANQEMEDNAA PHENNDAHPM IKGKEKDKHG CCIVM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca gccagaccgg aggggcagtc gccgtcaacg agatctcgca gcagcactcg accgagcaag cgatggctgc caaagaaatg gcagcggaga accaggtgca cgtgacgctg cccgaggagg tgagggagcg ccgccgctct cgtcaatcgt cgcaggcgcg taacgggtcc cacgaggcac cgatcatttc ctacggcgag gcggcgccga agcagacggt ctcgcgcgtg ttggtgcgga tgccgaatga ggtacgccgc aggcgaagcc tctctcgcga ggcccgtaag agccataccc acacgcagct ggaggagagc gagatgccga acgccacccc taaagccatg cccgaagccg ccgctatgtc gactgccgaa gatggtcgtg ttagcccgga gaagcagtgt ccagagccgg agctgcccgc caacggcttg gccatgcagc ccgctgtcga ggaggcagtc gatcatgagc cgccgacaga accctcaccc gcggccgaag aggccgagag atccgggagg ctcaccgacg cggcctctcg ggtggaaatg gagttgacag ggagcgactc cgctgctgtc atcgaggagg ctaaccagga aatggaggat aatgcggcgc ctcacgagaa caatgatgcg cacccgatga tcaagggcaa ggagaaggat aagcacggat gctgcatagt gatgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat