SSGCID: Target Page

LeinA.18745.a

Uncharacterized protein

CENTER ID:	LeinA.18745.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18745.a.B1.GE37558	full length		1	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.29.0350
RefSeq:	XP_001466555.1
UniProt:	A4I486

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDVDVVANF VVMTGATEDQ AIHYLSTYNF NLEDAVVAFQ VDNPHPSPPH TREDSPHYSS VDTGSPMSAP SPASHQVEGF AFPQAAQRPP TPPRQRALAS STAEAIQRLF ARPDYVVGSD RATFDAECEK AASRHCWVVV SVVDNSFPCE CFTRDIWASD AMRSLTSGSL FCYEINVTHT RGMALAEKYH VDNSNLPRMF MVDPVTQFKV QELPLISSEV WQFDSAVVVD AIMLFITNHD PPHDPFAGAD DQPQNEGGGG DSAESASASA SVKAPAVVDV DEGDSEAEMV SPPTISRSVA GAVASTTSTL EVGEPGPVVA PSPVTLDEYT VPEGTTGDAS SVFRLRCRLP HSSSTLQLRP DTPVARLIDY LAYLLYAEDT TRYAVPPRIS IFSGFPPKNM TAGEMEGITL STWDGVRSGD VLMVRVNA

NT Sequence

atgagcgacg tggacgtggt ggcgaatttc gtggtcatga cgggggccac cgaggatcaa gccatccact acctttccac gtacaacttc aacttggagg atgctgtcgt ggcgtttcag gtagacaatc ctcatccctc gccgccgcac acacgagagg actctccaca ctactccagc gtcgacacag ggtcacccat gagcgcgcca tcgccggcct cgcaccaagt ggagggcttt gcgttccccc aggcggctca gcgtccaccc acgccaccgc gacagcgagc tcttgcctcc tcgactgccg aagcgataca gcgcctcttc gcccgtcctg actacgtagt gggcagcgac agagccacct tcgacgctga gtgcgagaag gccgcatcgc gccactgctg ggtcgtcgtg tccgtcgtgg acaactcgtt cccctgcgag tgctttaccc gcgatatctg ggccagcgac gccatgcgct ccctcaccag cggcagcctg ttttgctatg agatcaacgt cacgcacacc cgcggcatgg ccctggccga aaagtaccac gtcgacaaca gcaatctgcc ccgcatgttc atggtcgatc cggtgactca gtttaaggtg caggagctgc cgctcatctc ctctgaggtg tggcagtttg acagtgccgt tgtagtggat gccatcatgc tcttcatcac gaaccacgac cccccgcacg atccgtttgc cggcgcggat gatcagccgc aaaacgaagg cggcggtggc gatagcgctg agtcggcgtc cgcgagtgca agcgtgaagg cgccggccgt ggtagacgtc gacgagggag acagcgaggc cgagatggta tcgcctccga cgatatcacg gagcgtagcc ggcgcggttg cttccacgac gagcacgctg gaggttggcg agccgggtcc agtcgtcgct ccctccccgg tgacgctgga cgagtacacg gtcccggagg gcaccaccgg cgatgcaagc agcgtctttc gactgcgatg ccgactgccg cattcgtctt ccactctgca gctgcgccct gacaccccgg tagcgaggct catcgactac ttggcgtatc tcttgtacgc ggaggacacc acgcgctatg cggttccgcc gcggattagc atcttcagtg ggtttcctcc aaagaacatg actgccgggg agatggaggg catcacgctg agcacgtggg acggcgtgcg gagcggcgat gtcttgatgg ttcgcgtgaa tgcgtga

Details for LeinA.18745.a.B1.GE37558

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	47kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

Validated NT Sequence

atcttactca cttacgcatt cacgcgaacc atcaagacat cgccgctccg cacgccgtcc cacgtgctca gcgtgatgcc ctccatctcc ccggcagtca tgttctttgg aggaaaccca ctgaagatgc taatccgcgg cggaaccgca tagcgcgtgg tgtcctccgc gtacaagaga tacgccaagt agtcgatgag cctcgctacc ggggtgtcag ggcgcagctg cagagtggaa gacgaatgcg gcagtcggca tcgcagtcga aagacgctgc ttgcatcgcc ggtggtgccc tccgggaccg tgtactcgtc cagcgtcacc ggggagggag cgacgactgg acccggctcg ccaacctcca gcgtgctcgt tgtggaagca accgcgccgg ctacgctccg tgatatcgtc ggaggcgata ccatctcggc ctcgctgtct ccctcgtcga cgtctaccac ggccggcgcc ttcacgcttg cactcgcgga cgccgactca gcgctatccc caccgccgcc ttcgttttgc ggctgatcat ccgcgccggc aaacggatcg tgcggggggt cgtggttcgt gatgaagagc atgatggcat ccactacaac ggcactgtca aactgccaca cctcagagga gatgagcggc agctcctgca ccttaaactg agtcaccgga tcgaccatga acatgcgggg cagattgctg ttgtcgacgt ggtacttttc ggccagggcc atgccgcggg tgtgcgtgac gttgatctca tagcaaaaca ggctgccgct ggtgagggag cgcatggcgt cgctggccca gatatcgcgg gtaaagcact cgcaggggaa cgagttgtcc acgacggaca cgacgaccca gcagtggcgc gatgcggcct tctcgcactc agcgtcgaag gtggctctgt cgctgcccac tacgtagtca ggacgggcga agaggcgctg tatcgcttcg gcagtcgagg aggcaagagc tcgctgtcgc ggtggcgtgg gtggacgctg agccgcctgg gggaacgcaa agccctccac ttggtgcgag gccggcgatg gcgcgctcat gggtgaccct gtgtcgacgc tggagtagtg tggagagtcc tctcgtgtgt gcggcggcga gggatgagga ttgtctacct gaaacgccac gacagcatcc tccaagttga agttgtacgt ggaaaggtag tggatggctt gatcctcggt ggcccccgtc atgaccacga aattcgccac cacgtccacg tcgctcatat ggtggtggtg gtggtgagcc

Expected Protein Sequence

MAHHHHHHMS DVDVVANFVV MTGATEDQAI HYLSTYNFNL EDAVVAFQVD NPHPSPPHTR EDSPHYSSVD TGSPMSAPSP ASHQVEGFAF PQAAQRPPTP PRQRALASST AEAIQRLFAR PDYVVGSDRA TFDAECEKAA SRHCWVVVSV VDNSFPCECF TRDIWASDAM RSLTSGSLFC YEINVTHTRG MALAEKYHVD NSNLPRMFMV DPVTQFKVQE LPLISSEVWQ FDSAVVVDAI MLFITNHDPP HDPFAGADDQ PQNEGGGGDS AESASASASV KAPAVVDVDE GDSEAEMVSP PTISRSVAGA VASTTSTLEV GEPGPVVAPS PVTLDEYTVP EGTTGDASSV FRLRCRLPHS SSTLQLRPDT PVARLIDYLA YLLYAEDTTR YAVPPRISIF SGFPPKNMTA GEMEGITLST WDGVRSGDVL MVRVNA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acgtggacgt ggtggcgaat ttcgtggtca tgacgggggc caccgaggat caagccatcc actacctttc cacgtacaac ttcaacttgg aggatgctgt cgtggcgttt caggtagaca atcctcatcc ctcgccgccg cacacacgag aggactctcc acactactcc agcgtcgaca cagggtcacc catgagcgcg ccatcgccgg cctcgcacca agtggagggc tttgcgttcc cccaggcggc tcagcgtcca cccacgccac cgcgacagcg agctcttgcc tcctcgactg ccgaagcgat acagcgcctc ttcgcccgtc ctgactacgt agtgggcagc gacagagcca ccttcgacgc tgagtgcgag aaggccgcat cgcgccactg ctgggtcgtc gtgtccgtcg tggacaactc gttcccctgc gagtgcttta cccgcgatat ctgggccagc gacgccatgc gctccctcac cagcggcagc ctgttttgct atgagatcaa cgtcacgcac acccgcggca tggccctggc cgaaaagtac cacgtcgaca acagcaatct gccccgcatg ttcatggtcg atccggtgac tcagtttaag gtgcaggagc tgccgctcat ctcctctgag gtgtggcagt ttgacagtgc cgttgtagtg gatgccatca tgctcttcat cacgaaccac gaccccccgc acgatccgtt tgccggcgcg gatgatcagc cgcaaaacga aggcggcggt ggcgatagcg ctgagtcggc gtccgcgagt gcaagcgtga aggcgccggc cgtggtagac gtcgacgagg gagacagcga ggccgagatg gtatcgcctc cgacgatatc acggagcgta gccggcgcgg ttgcttccac gacgagcacg ctggaggttg gcgagccggg tccagtcgtc gctccctccc cggtgacgct ggacgagtac acggtcccgg agggcaccac cggcgatgca agcagcgtct ttcgactgcg atgccgactg ccgcattcgt cttccactct gcagctgcgc cctgacaccc cggtagcgag gctcatcgac tacttggcgt atctcttgta cgcggaggac accacgcgct atgcggttcc gccgcggatt agcatcttca gtgggtttcc tccaaagaac atgactgccg gggagatgga gggcatcacg ctgagcacgt gggacggcgt gcggagcggc gatgtcttga tggttcgcgt gaatgcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at