SSGCID: Target Page

LeinA.18744.a

Uncharacterized protein

CENTER ID:	LeinA.18744.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18744.a.B1.GE37557	full length		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18744.a.B1.PS02017	full length		1	384	unavailable

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.27.2310
RefSeq:	XP_001466471.1
UniProt:	A4I338

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSAATLSID AHKWTETIEA AGTDCLCSAV SAKNITHVLS TATVRAPQKH LCAAVSNFVP AMLENLHGVS ILTALVRYGT TATVEQIASK LSAADEGVWS FMAAPKKELT KCLSHLLERM VYREDCTGES YNALLGRLKA VKKQSLMTSS FTLPAAARLA LVDDAFATGL LSSSEAQKAL GKSCQHAATA AAAEEFCRIL FERPKDKAAG DFVWKALAAS MKADAKAHPR EAILALLAAH GPVPLVNKMA DAMAQWSTVR ELCTRDSYAH IVARLLERCD DERAGNRLVA AVIMQEADVT ERVGARKAAQ HHLLAVLAAK SSYAQTLEKR LGTSQTKRLA AAKVRFANAT QSKATTTQQA ILEKLKKLHS TTTSSVAGTK RARE

NT Sequence

atgccttccg cagcgacgct ctcgatcgac gcgcacaagt ggactgagac gatcgaggcg gcaggcactg actgcctctg ctctgctgtc tctgcgaaga acattaccca tgtcctgtcg accgccacgg tacgagcgcc gcagaagcat ctttgcgccg cggtgagcaa ctttgtgcca gcgatgctag agaaccttca cggcgtctcg atcttgacag cgctggtgcg ctatggcacc acggcgaccg ttgagcaaat tgccagcaaa ctcagtgcag cggacgaggg cgtatggtcg ttcatggctg ctccaaagaa ggagctgacc aaatgcctgt cgcacctgct tgagcggatg gtgtaccggg aggactgcac cggcgagtcc tacaacgcac ttttgggccg gctgaaggct gtcaagaagc agtcgctgat gacctcttcc tttacactgc ccgccgctgc tcgcctcgcg ctggtggatg acgcattcgc tacagggctc ctgtcctcga gtgaagcgca gaaggcgttg ggcaagtcgt gtcagcacgc cgccaccgcc gcagccgcgg aggagttttg ccgcatacta tttgagaggc caaaggacaa ggcagccggt gactttgttt ggaaagctct ggcggctagc atgaaggcag acgccaaggc gcacccacgc gaggccattc tcgccctcct cgcggcgcac ggacccgtgc cgctggtgaa caagatggcc gacgcgatgg cacagtggtc gacggtgcgc gaactgtgca cccgtgactc ttacgcgcac attgtcgccc gcctgctgga gcgctgcgac gacgagaggg ccggcaacag gttggtcgct gccgtcatca tgcaagaggc ggacgtgaca gagcgggtgg gcgcgcgcaa ggcggcccag caccatctcc tggcagtgct cgcagcgaag tccagctacg cgcagaccct agagaagcgt cttggtacct cgcagacgaa gcgcctggcg gcggcgaagg tacgcttcgc aaacgccacg cagtcgaagg cgaccacgac gcagcaggcg atcctcgaga agctgaagaa gctacacagc accaccactt cctccgtcgc cgggacgaag cgcgcgcgtg agtga

Details for LeinA.18744.a.B1.GE37557

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MPSAATLSID AHKWTETIEA AGTDCLCSAV SAKNVTHVLS TATVRAPQKH LCAAVSNFVP AMLENLHGVS ILTALVRYGT TATVEQIASK LSAADEGVWS FMAAPKKELT KCLSHLLERM VYREDCTGES YNALLGRLKA VKKQSLMTSS FTLPAAARLA LVDDAFATGL LSSSEAQKAL GKSFQHAATA AAAEEFCRIL FERPKDNAAG DFVWKALAAS MKADAKAHPR EAILALLAAH GPVPLVNKMA DAMAQWSTVR ELCTRDSYAH IVARLLERCD DERAGNRLVA AVIMQEADVT ERVGARKAAQ HHLLAVLAAK SSYAQTLEKR LGTSQTKRLA AAKVRFANAT QSKATTTQQA ILEKLKKLHS TTTSSVAGTK RARE

Validated NT Sequence

tcttactcac ttactcacgc gcgcgcttcg tcccggcgac ggaggaagtg gtggtgctgt gtagcttctt cagcttctcg aggatcgcct gctgcgtcgt ggtcgccttc gactgcgtgg cgtttgcgaa gcgtaccttc gccgccgcca ggcgcttcgt ctgcgaggta ccaagacgct tctctagggt ctgcgcgtag ctggacttcg ctgcgagcac tgccaggaga tggtgctggg ccgccttgcg cgcgcccacc cgctctgtca cgtccgcctc ttgcatgatg acggcagcga ccaacctgtt gccggccctc tcgtcgtcgc agcgctccag caggcgggcg acaatgtgcg cgtaagagtc acgggtgcac agttcgcgca ccgtcgacca ctgtgccatc gcgtcggcca tcttgttcac cagcggcacg ggtccgtgcg ccgcgaggag ggcgagaatg gcctcgcgtg ggtgcgcctt ggcgtctgcc ttcatgctag ccgccagagc tttccaaaca aagtcaccgg ccgcgttgtc ctttggcctc tcaaatagta tgcggcaaaa ctcctccgcg gctgcggcgg tggcggcgtg ctgaaacgac ttgcccaacg ccttctgcgc ttcactcgag gacaggagcc ctgtagcaaa tgcgtcatcc accagcgcga ggcgagcagc ggcgggcagt gtaaaggaag aggtcatcag cgactgcttc ttgacagcct tcagccggcc caaaagtgcg ttgtaggact cgccggtgca gtcctcccgg tacaccatcc gctcaagcag gtgcgacagg catttggtca gctccttctt tggagcagcc atgaacgacc atacgccctc gtccgctgca ctgagtttgc tggcaatttg ctcaacggtc gccgtggtgc catagcgcac cagcgctgtc aagatcgaga cgccgtgaag gttctctagc atcgctggca caaagttgct caccgcggcg caaagatgct tctgcggcgc tcgcaccgtg gcggtcgaca ggacatgggt aacgttcttc gcagagacag cagagcagag gcagtcagtg cctgccgcct cgatcgtctc agtccacttg tgcgcgtcga tcgagagcgt cgctgcggaa ggcatatggt ggtg

Expected Protein Sequence

MAHHHHHHMP SAATLSIDAH KWTETIEAAG TDCLCSAVSA KNITHVLSTA TVRAPQKHLC AAVSNFVPAM LENLHGVSIL TALVRYGTTA TVEQIASKLS AADEGVWSFM AAPKKELTKC LSHLLERMVY REDCTGESYN ALLGRLKAVK KQSLMTSSFT LPAAARLALV DDAFATGLLS SSEAQKALGK SCQHAATAAA AEEFCRILFE RPKDKAAGDF VWKALAASMK ADAKAHPREA ILALLAAHGP VPLVNKMADA MAQWSTVREL CTRDSYAHIV ARLLERCDDE RAGNRLVAAV IMQEADVTER VGARKAAQHH LLAVLAAKSS YAQTLEKRLG TSQTKRLAAA KVRFANATQS KATTTQQAIL EKLKKLHSTT TSSVAGTKRA RE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctt ccgcagcgac gctctcgatc gacgcgcaca agtggactga gacgatcgag gcggcaggca ctgactgcct ctgctctgct gtctctgcga agaacattac ccatgtcctg tcgaccgcca cggtacgagc gccgcagaag catctttgcg ccgcggtgag caactttgtg ccagcgatgc tagagaacct tcacggcgtc tcgatcttga cagcgctggt gcgctatggc accacggcga ccgttgagca aattgccagc aaactcagtg cagcggacga gggcgtatgg tcgttcatgg ctgctccaaa gaaggagctg accaaatgcc tgtcgcacct gcttgagcgg atggtgtacc gggaggactg caccggcgag tcctacaacg cacttttggg ccggctgaag gctgtcaaga agcagtcgct gatgacctct tcctttacac tgcccgccgc tgctcgcctc gcgctggtgg atgacgcatt cgctacaggg ctcctgtcct cgagtgaagc gcagaaggcg ttgggcaagt cgtgtcagca cgccgccacc gccgcagccg cggaggagtt ttgccgcata ctatttgaga ggccaaagga caaggcagcc ggtgactttg tttggaaagc tctggcggct agcatgaagg cagacgccaa ggcgcaccca cgcgaggcca ttctcgccct cctcgcggcg cacggacccg tgccgctggt gaacaagatg gccgacgcga tggcacagtg gtcgacggtg cgcgaactgt gcacccgtga ctcttacgcg cacattgtcg cccgcctgct ggagcgctgc gacgacgaga gggccggcaa caggttggtc gctgccgtca tcatgcaaga ggcggacgtg acagagcggg tgggcgcgcg caaggcggcc cagcaccatc tcctggcagt gctcgcagcg aagtccagct acgcgcagac cctagagaag cgtcttggta cctcgcagac gaagcgcctg gcggcggcga aggtacgctt cgcaaacgcc acgcagtcga aggcgaccac gacgcagcag gcgatcctcg agaagctgaa gaagctacac agcaccacca cttcctccgt cgccgggacg aagcgcgcgc gtgagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for LeinA.18744.a.B1.PS02017

PURIFICATION DATe:	3/12/2014
CONCENTRATION:	27.5mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTT CCGCAGCGAC GCTCTCGATC GACGCGCACA AGTGGACTGA GACGATCGAG GCGGCAGGCA CTGACTGCCT CTGCTCTGCT GTCTCTGCGA AGAACATTAC CCATGTCCTG TCGACCGCCA CGGTACGAGC GCCGCAGAAG CATCTTTGCG CCGCGGTGAG CAACTTTGTG CCAGCGATGC TAGAGAACCT TCACGGCGTC TCGATCTTGA CAGCGCTGGT GCGCTATGGC ACCACGGCGA CCGTTGAGCA AATTGCCAGC AAACTCAGTG CAGCGGACGA GGGCGTATGG TCGTTCATGG CTGCTCCAAA GAAGGAGCTG ACCAAATGCC TGTCGCACCT GCTTGAGCGG ATGGTGTACC GGGAGGACTG CACCGGCGAG TCCTACAACG CACTTTTGGG CCGGCTGAAG GCTGTCAAGA AGCAGTCGCT GATGACCTCT TCCTTTACAC TGCCCGCCGC TGCTCGCCTC GCGCTGGTGG ATGACGCATT CGCTACAGGG CTCCTGTCCT CGAGTGAAGC GCAGAAGGCG TTGGGCAAGT CGTGTCAGCA CGCCGCCACC GCCGCAGCCG CGGAGGAGTT TTGCCGCATA CTATTTGAGA GGCCAAAGGA CAAGGCAGCC GGTGACTTTG TTTGGAAAGC TCTGGCGGCT AGCATGAAGG CAGACGCCAA GGCGCACCCA CGCGAGGCCA TTCTCGCCCT CCTCGCGGCG CACGGACCCG TGCCGCTGGT GAACAAGATG GCCGACGCGA TGGCACAGTG GTCGACGGTG CGCGAACTGT GCACCCGTGA CTCTTACGCG CACATTGTCG CCCGCCTGCT GGAGCGCTGC GACGACGAGA GGGCCGGCAA CAGGTTGGTC GCTGCCGTCA TCATGCAAGA GGCGGACGTG ACAGAGCGGG TGGGCGCGCG CAAGGCGGCC CAGCACCATC TCCTGGCAGT GCTCGCAGCG AAGTCCAGCT ACGCGCAGAC CCTAGAGAAG CGTCTTGGTA CCTCGCAGAC GAAGCGCCTG GCGGCGGCGA AGGTACGCTT CGCAAACGCC ACGCAGTCGA AGGCGACCAC GACGCAGCAG GCGATCCTCG AGAAGCTGAA GAAGCTACAC AGCACCACCA CTTCCTCCGT CGCCGGGACG AAGCGCGCGC GTGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT