SSGCID: Target Page

LeinA.18741.a

Uncharacterized protein

CENTER ID:	LeinA.18741.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18741.a.B1.GE37555	full length		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18741.a.B1.PS02002	full length		1	282

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.08.0490
RefSeq:	XP_001463387.1
UniProt:	A4HTJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAQLTRDEVH TYAEAMIAAI KTPAFQERVR AAMSREPTPT DAEAVMKRYE EVQADYFTNH YNAEAGGICS SVSAGAVGRG TGDACGRAKS SQDASGVLAQ QVDNMSLEKA SDAPKLDGVE IVEQLKNAVR MYKEKETVRL ITELCLLIES QVTALPAAVP ALKHLYSNSV YGSSVMEQAQ AASARAGGGE NVLPVAHPSP QVMQMMDMAM RTLSPKQRET LERVQRATMN GSPPLPEDMQ DMLLIQRQLG AFMQTMQRFG QMPSGRGRGS GGRGGCGGRG AP

NT Sequence

atggcgcaac ttacgagaga tgaggtccac acctacgccg aggccatgat cgccgccatc aagacacctg cgtttcagga gcgcgtccgt gctgcgatga gccgtgagcc gaccccgacg gatgctgagg ccgtcatgaa gcgctatgaa gaagtacagg cggactactt caccaaccac tacaacgccg aagccggcgg catctgcagc agcgtcagcg ccggtgcagt gggcagaggt actggcgacg cgtgtggtcg tgctaagagc tctcaagatg catccggggt actggcgcag caggtggaca acatgagcct cgagaaggca agcgatgcgc cgaagctgga cggcgtcgaa attgtggagc agctcaagaa cgcggtacgc atgtacaagg agaaggagac ggtgcggctc atcacggagc tctgcttgct gatcgagtcg caggtgacgg cgctcccagc ggccgtgcca gccctgaagc acctctacag caattccgtg tatggctcca gtgtgatgga gcaggcacag gcggcgtccg cgcgggccgg cggcggtgag aacgtgttac ccgtcgcgca cccgtctcca caggtgatgc aaatgatgga catggcgatg cgcaccctca gcccaaagca acgcgagacc ctcgagcgag tgcagcgggc aacgatgaac ggcagcccgc cgttgccgga ggatatgcaa gacatgctcc tcattcagcg tcagcttggc gccttcatgc agacgatgca gcgcttcggc cagatgccga gcggcagggg tcgtggcagt ggtggtcgcg gcggctgtgg tgggcgaggg gccccgtag

Details for LeinA.18741.a.B1.GE37555

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA QLTRDEVHTY AEAMIAAIKT PAFQERVRAA MSREPTPTDA EAVMKRYEEV QADYFTNHYN AEAGGICSSV SAGAVGRGTG DACGRAKSSQ DASGVLAQQV DNMSLEKASD APKLDGVEIV EQLKNAVRMY KEKETVRLIT ELCLLIESQV TALPAAVPAL KHLYSNSVYG SSAMEQAQAA SARAGGGENV LPVAHPSPQV MQMMDMAMRT LSPKQRETLE RVQRATMNGS PPLPEDMQDM LLIQRQLGAF MQTMQRFGQM PSGRGRGSGG RGGCGGRGAP

Validated NT Sequence

atggctcacc accaccacca ccatatggcg caacttacga gagatgaggt ccacacctac gccgaggcca tgatcgccgc catcaagaca cctgcgtttc aggagcgcgt ccgtgctgcg atgagccgtg agccgacccc gacggatgct gaggccgtca tgaagcgcta tgaagaagta caggcggact acttcaccaa ccactacaac gccgaagccg gcggcatctg cagcagcgtc agcgccggtg cagtgggcag aggtactggc gacgcgtgtg gtcgtgctaa gagctctcaa gatgcatccg gggtactggc gcagcaggtg gacaacatga gcctcgagaa ggcaagcgat gcgccgaagc tggacggcgt cgaaattgtg gagcagctca agaacgcggt acgcatgtac aaggagaagg agacggtgcg gctcatcacg gagctctgct tgctgatcga gtcgcaggtg acggcgctcc cagcggccgt gccagccctg aagcacctct acagcaattc cgtgtatggc tccagtgcga tggagcaggc gcaggcggcg tccgcgcggg ccggcggcgg tgagaacgtg ttacccgtcg cgcacccgtc tccacaggtg atgcaaatga tggacatggc gatgcgcacc ctcagcccaa agcaacgcga gaccctcgag cgagtgcagc gggcaacgat gaacggcagc ccgccgttgc cggaggatat gcaagacatg ctcctcattc agcgtcagct tggcgccttc atgcagacga tgcagcgctt cggccagatg ccgagcggca ggggtcgtgg cagtggtggt cgcggcggct gtggtgggcg aggggccccg taagtgagta agat

Expected Protein Sequence

MAHHHHHHMA QLTRDEVHTY AEAMIAAIKT PAFQERVRAA MSREPTPTDA EAVMKRYEEV QADYFTNHYN AEAGGICSSV SAGAVGRGTG DACGRAKSSQ DASGVLAQQV DNMSLEKASD APKLDGVEIV EQLKNAVRMY KEKETVRLIT ELCLLIESQV TALPAAVPAL KHLYSNSVYG SSVMEQAQAA SARAGGGENV LPVAHPSPQV MQMMDMAMRT LSPKQRETLE RVQRATMNGS PPLPEDMQDM LLIQRQLGAF MQTMQRFGQM PSGRGRGSGG RGGCGGRGAP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc aacttacgag agatgaggtc cacacctacg ccgaggccat gatcgccgcc atcaagacac ctgcgtttca ggagcgcgtc cgtgctgcga tgagccgtga gccgaccccg acggatgctg aggccgtcat gaagcgctat gaagaagtac aggcggacta cttcaccaac cactacaacg ccgaagccgg cggcatctgc agcagcgtca gcgccggtgc agtgggcaga ggtactggcg acgcgtgtgg tcgtgctaag agctctcaag atgcatccgg ggtactggcg cagcaggtgg acaacatgag cctcgagaag gcaagcgatg cgccgaagct ggacggcgtc gaaattgtgg agcagctcaa gaacgcggta cgcatgtaca aggagaagga gacggtgcgg ctcatcacgg agctctgctt gctgatcgag tcgcaggtga cggcgctccc agcggccgtg ccagccctga agcacctcta cagcaattcc gtgtatggct ccagtgtgat ggagcaggca caggcggcgt ccgcgcgggc cggcggcggt gagaacgtgt tacccgtcgc gcacccgtct ccacaggtga tgcaaatgat ggacatggcg atgcgcaccc tcagcccaaa gcaacgcgag accctcgagc gagtgcagcg ggcaacgatg aacggcagcc cgccgttgcc ggaggatatg caagacatgc tcctcattca gcgtcagctt ggcgccttca tgcagacgat gcagcgcttc ggccagatgc cgagcggcag gggtcgtggc agtggtggtc gcggcggctg tggtgggcga ggggccccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for LeinA.18741.a.B1.PS02002

PURIFICATION DATe:	2/19/2014
CONCENTRATION:	23.7mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMA QLTRDEVHTY AEAMIAAIKT PAFQERVRAA MSREPTPTDA EAVMKRYEEV QADYFTNHYN AEAGGICSSV SAGAVGRGTG DACGRAKSSQ DASGVLAQQV DNMSLEKASD APKLDGVEIV EQLKNAVRMY KEKETVRLIT ELCLLIESQV TALPAAVPAL KHLYSNSVYG SSVMEQAQAA SARAGGGENV LPVAHPSPQV MQMMDMAMRT LSPKQRETLE RVQRATMNGS PPLPEDMQDM LLIQRQLGAF MQTMQRFGQM PSGRGRGSGG RGGCGGRGAP

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGC AACTTACGAG AGATGAGGTC CACACCTACG CCGAGGCCAT GATCGCCGCC ATCAAGACAC CTGCGTTTCA GGAGCGCGTC CGTGCTGCGA TGAGCCGTGA GCCGACCCCG ACGGATGCTG AGGCCGTCAT GAAGCGCTAT GAAGAAGTAC AGGCGGACTA CTTCACCAAC CACTACAACG CCGAAGCCGG CGGCATCTGC AGCAGCGTCA GCGCCGGTGC AGTGGGCAGA GGTACTGGCG ACGCGTGTGG TCGTGCTAAG AGCTCTCAAG ATGCATCCGG GGTACTGGCG CAGCAGGTGG ACAACATGAG CCTCGAGAAG GCAAGCGATG CGCCGAAGCT GGACGGCGTC GAAATTGTGG AGCAGCTCAA GAACGCGGTA CGCATGTACA AGGAGAAGGA GACGGTGCGG CTCATCACGG AGCTCTGCTT GCTGATCGAG TCGCAGGTGA CGGCGCTCCC AGCGGCCGTG CCAGCCCTGA AGCACCTCTA CAGCAATTCC GTGTATGGCT CCAGTGTGAT GGAGCAGGCA CAGGCGGCGT CCGCGCGGGC CGGCGGCGGT GAGAACGTGT TACCCGTCGC GCACCCGTCT CCACAGGTGA TGCAAATGAT GGACATGGCG ATGCGCACCC TCAGCCCAAA GCAACGCGAG ACCCTCGAGC GAGTGCAGCG GGCAACGATG AACGGCAGCC CGCCGTTGCC GGAGGATATG CAAGACATGC TCCTCATTCA GCGTCAGCTT GGCGCCTTCA TGCAGACGAT GCAGCGCTTC GGCCAGATGC CGAGCGGCAG GGGTCGTGGC AGTGGTGGTC GCGGCGGCTG TGGTGGGCGA GGGGCCCCGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT