SSGCID: Target Page

LeinA.18740.a

Uncharacterized protein

CENTER ID:	LeinA.18740.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18740.a.B1.GE37554	full length		1	1022

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.35.5000
RefSeq:	XP_003392821.1
UniProt:	E9AHX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGLDSHGKA MGEAQSDRQM SSSSLTAASR ATPNPAAADG GAAAHAGGSP VDNEMDEHVP PVIAHVRKNF GVLAPTIEPF IYRSGCLNKS VVDVYNNLSR NYIAIVDAAL KQVGFEEFTM QMQIMLTQIL SEEFGEDTEY LVNTILTYTG ADSLFVSLLS KSMLDQVITY AQSIMSGNMY GMSYNMQSGG GGGGRGSEGV GNSYYRGAQQ HHGYRGAGEN GSSRDLRGSS VAASPPHQDG AAPQKPYRSS GLPPHSSDPG RERSSDDLRA TSDVISQREE SRDWGGGASV PPRGATATGG IHVDSSVGPR GGAAPMSGMG AGAASRTEVS VSRATSETTS GAGVVPRHTH HHFSSSTAAA APAAKPSSME RGGMSGAPQH NLLPPRASPA SSQPRVSTPG APSVSQATTT SPMPTMGRGG PGIPMHHHHH HLGIGAMATN SAAASDWAAS NSVSGSTPNI NAAGQRTLTP PTPQSASMQQ HQHQHMQQQS QQQLPQPQQP TRTMNIPAPL SVQRRTEKNA AAAAAAAAPA AAPANGSAPA NSSGLSSSSA AVEGGAAPRN MSAPLGGSAN VPAPRGAQSL PHHQHHMPVS HHHHHHHHFV ASPPAVTAAV QPPSATHSPP ASEERVATIP AESTGTPAPE TNLPANQASA STPAPARSPP ATSVSTTSAP PPALRGLGRI PLHHHHHHMH LMMNKVDAAS APQRQPQPIP ATLQLQPQPH LPQPQQRVAT AIDVHDQQQQ ERTNAANGRW PNQKEELRGM LLTARVSESA VTYLLTDVNI EELRGLKYVD FEKKLRGGIP LMLDRQRIRQ VLSSSAAVQS QSSDNDDSAT SSNRKTATSG VGAEEEEAGG SGLPRRPPAI STGVQSPVTS HQSSPISKAG VQVPQPRTKM QQQQQQQTQQ SINGGSAVAS PADASFSSPE KESDKREGGT KTGSPTTASG APTSPVPAST PAPSPNSSYS PSTRNEDPED SREEEQRRGS GRRGGKRGGL GRGGFSSSRV CRFFGTAEGC QYGDKCHYMH SK

NT Sequence

atgtccggct tggattccca tggcaaggcc atgggagagg cgcaatcaga taggcaaatg agctcctctt cgctcaccgc tgcatcacga gccaccccca acccagccgc agcggacggt ggcgctgccg cccatgccgg tggctcccct gtggataacg agatggacga gcacgtgcca ccggtcattg cccacgtccg caagaacttc ggcgtactcg cccctaccat tgagcccttc atctaccgta gcggttgcct caacaagtca gtggtagacg tgtacaacaa cctgtcgcgc aactacattg ccattgtaga tgctgccctc aagcaggtag gatttgaaga gttcacgatg cagatgcaga tcatgctgac ccagatcctg tctgaggagt ttggcgaaga tacggagtac ctcgtcaaca ccatcctaac gtacaccggc gccgactccc tgttcgtctc ccttctctcg aaatctatgc tggaccaggt catcacgtac gcgcaatcaa tcatgagcgg caacatgtac ggcatgagct acaacatgca gagcggcgga ggcggcggtg gtcgcggctc tgagggtgtc ggtaatagtt actaccgtgg tgcccaacag catcacggat accgtggtgc cggtgaaaac ggctcatcgc gcgatctccg cggcagctcc gtcgccgcca gccctcctca tcaggacggg gccgcaccac agaagccgta ccgcagcagc ggcctcccac cccactccag cgacccaggc agggagcgca gcagcgatga tttgcgggcc accagcgatg taatctcaca acgcgaggag agtcgggatt gggggggagg cgcttcagtg ccgcctcgcg gcgctactgc gaccgggggc atccacgtag attctagtgt tggtccccgg ggcggtgccg ctcccatgag cgggatgggc gcaggtgcgg ccagtcgcac agaggtgtcc gtgagccggg ccacgagcga gactacatct ggagcaggag tagtgccacg tcacacgcac catcacttct cctcttctac agcggccgcc gctcccgccg cgaagccgtc gtcgatggag cgaggtggga tgagcggggc tccgcagcac aacctcctac ccccgcgagc ttcaccggcg agcagccagc cgcgcgtgtc cacgccaggg gcgccaagcg tgtcgcaggc caccaccact tccccgatgc cgacgatggg acgcggcggg ccgggcatcc cgatgcacca ccatcaccat cacttgggca taggcgcaat ggcaaccaac agtgccgcgg ccagtgactg ggccgccagc aacagcgtat ctgggtccac accaaacatc aacgccgctg ggcagcgcac cctgacccca ccaacgccgc agtccgcatc gatgcagcag catcaacatc agcacatgca gcagcaatcc cagcaacagc tgccgcagcc gcagcagccg acgcgcacca tgaacattcc cgcccccttg agtgtgcagc gccgcacaga aaagaacgcg gcggcagccg cagcagccgc tgccccggca gcagcacccg ctaacggcag cgcgcctgcc aattcgtctg gcctcagtag tagtagcgca gctgtagagg gaggggctgc gccacgcaac atgtctgcgc ctctcggcgg gagcgcgaac gtcccggcgc cgcgcggggc gcagtcactc ccccatcacc agcaccacat gcccgtctct caccaccatc accatcacca tcatttcgtc gcgtccccgc ccgcggtcac tgcagctgtg cagccgccct ccgctacgca ctcgccacca gcttccgagg agcgcgttgc gaccattccc gccgaatcca cgggcacgcc ggcgccggag acgaacttac ccgctaatca ggcctctgca tcgaccccag ccccggccag gtcccctcct gccaccagcg tctcgacgac ttccgcaccc ccgccagcgt tgcgtggcct aggccgcatc cccttgcatc atcaccacca tcatatgcac ttgatgatga ataaagtaga tgcggcctcg gcgccgcaac ggcagccgca accgattcct gcgacgttgc agctgcagcc acagccgcac ctgccgcagc cgcagcagcg cgtggccacc gcgatcgacg tgcatgacca gcagcagcag gagcggacga acgccgccaa cggtcgctgg cccaatcaaa aggaggaact gcgaggcatg ctgctgaccg cgcgcgtgtc ggagagcgcg gtcacgtact tgctgacgga cgtgaacata gaagagctgc gtggcttgaa gtacgtagat tttgagaaga agcttcgcgg tggtattccg ctgatgttag atcgccagcg catccgtcag gtgctgtcca gcagcgccgc tgtgcagagt cagtcgagcg acaacgacga ctccgcgacg agctccaacc gaaagactgc aaccagcgga gtaggcgcag aggaggagga ggcgggcggg tctggactgc ctcggcgacc cccggccatt tccactggcg tgcagagccc ggtgacgtcg caccagtcct cgcctattag caaggctgga gtgcaggtgc cgcagcctcg aacgaagatg cagcagcagc aacagcagca gacgcagcaa tccatcaacg gcggcagtgc cgtggcctcg ccagcggatg cgagcttttc ctcaccggag aaggagtccg acaagcgtga gggcggcacc aagacgggga gcccgacgac ggccagcggc gcacccacct ccccggtgcc ggcctcaact ccggcgcctt cccccaacag tagctacagc cctagtactc gcaacgagga tcccgaggat agccgtgagg aggagcagcg gcgtggtagc ggacgccgtg gcgggaagcg tggcggtctg gggcgcggcg gcttcagctc atcgcgcgtg tgcaggttct tcggtactgc agaggggtgc cagtacggag acaagtgcca ctacatgcac agcaagtaa

Details for LeinA.18740.a.B1.GE37554

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	107kDa
OBSERVED MW:	115kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	30

Validated AA Sequence

Validated NT Sequence

aannattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgtccggctt ggattcccat ggcaaggcca tgggagaggc gcaatcagat aggcaaatga gctcctcttc gctcaccgct gcatcacgag ccactcccaa cccagccgca gcggacggtg gcgctgccgc ccatgccggt ggctcccctg tggataacga gatggacgag cacgtgccac cggtcattgc ccacgtccgc aagaacttcg gcgtactcgc ccctaccatt gagcccttca tctaccgtag cggttgcctc aacaagtcag tggtagacgt gtacaacaac ctgtcgcgca actacattgc cattgtagat gctgccctca agcaggtagg atttgaagag ttcacgatgc agatgcagat catgctgacc cagatcctgt ctgaggagtt tggcgaagat acggagtacc tcgtcaacac catcctaacg tacaccggcg ccgactccct gttcgtctcc cttctctcga aatctatgct ggaccaggtc atcacgtacg cgcaatcaat catgagcggc aacatgtacg ggatgagcta caacatgcag agcggcggag gcggcggtgg tcgcggctct gagggtgtcg gtaatagtta ctaccgtggt gcccaacagc atcacggata ccgtggtgcc ggtgaaaacg gctcatcgcg cgatctccgc ggcagctccg tcgccgccag ccctcctcat caggacgggg ccgcaccaca gaagccgtac cgcagcagcg gcctcccacc ccactccagc gacccaggca gggagcgcag cagcgatgat ttgcgggcca ccagcgatgt aatctcacaa cgcgaggaga gtcgggattg gggggggagg cgcttcagtg ccgcctcgcg gcgctactgc gaccgggggc atccacgtag attctagtgt tggtccccgg ggcggtgccg ctcccatgag cgggatgggc gcagntgcgg ccagtcgcac agaggtgtcc gtgagccggg ccacgagcga gactacntct ggagcngnnt antgcncgtn acanncacat nantntctnt nnncagnggn ncnntccnnc gcnnncntcn tcnangannn nnnnggatna nnggggnnnc nnnnncnanc nnctanccnn nnnnnnnnnn nnnagnnngc cnngcngnnt tccnncctca gggggncccn acangcggnt

Expected Protein Sequence

MAHHHHHHMS GLDSHGKAMG EAQSDRQMSS SSLTAASRAT PNPAAADGGA AAHAGGSPVD NEMDEHVPPV IAHVRKNFGV LAPTIEPFIY RSGCLNKSVV DVYNNLSRNY IAIVDAALKQ VGFEEFTMQM QIMLTQILSE EFGEDTEYLV NTILTYTGAD SLFVSLLSKS MLDQVITYAQ SIMSGNMYGM SYNMQSGGGG GGRGSEGVGN SYYRGAQQHH GYRGAGENGS SRDLRGSSVA ASPPHQDGAA PQKPYRSSGL PPHSSDPGRE RSSDDLRATS DVISQREESR DWGGGASVPP RGATATGGIH VDSSVGPRGG AAPMSGMGAG AASRTEVSVS RATSETTSGA GVVPRHTHHH FSSSTAAAAP AAKPSSMERG GMSGAPQHNL LPPRASPASS QPRVSTPGAP SVSQATTTSP MPTMGRGGPG IPMHHHHHHL GIGAMATNSA AASDWAASNS VSGSTPNINA AGQRTLTPPT PQSASMQQHQ HQHMQQQSQQ QLPQPQQPTR TMNIPAPLSV QRRTEKNAAA AAAAAAPAAA PANGSAPANS SGLSSSSAAV EGGAAPRNMS APLGGSANVP APRGAQSLPH HQHHMPVSHH HHHHHHFVAS PPAVTAAVQP PSATHSPPAS EERVATIPAE STGTPAPETN LPANQASAST PAPARSPPAT SVSTTSAPPP ALRGLGRIPL HHHHHHMHLM MNKVDAASAP QRQPQPIPAT LQLQPQPHLP QPQQRVATAI DVHDQQQQER TNAANGRWPN QKEELRGMLL TARVSESAVT YLLTDVNIEE LRGLKYVDFE KKLRGGIPLM LDRQRIRQVL SSSAAVQSQS SDNDDSATSS NRKTATSGVG AEEEEAGGSG LPRRPPAIST GVQSPVTSHQ SSPISKAGVQ VPQPRTKMQQ QQQQQTQQSI NGGSAVASPA DASFSSPEKE SDKREGGTKT GSPTTASGAP TSPVPASTPA PSPNSSYSPS TRNEDPEDSR EEEQRRGSGR RGGKRGGLGR GGFSSSRVCR FFGTAEGCQY GDKCHYMHSK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg gcttggattc ccatggcaag gccatgggag aggcgcaatc agataggcaa atgagctcct cttcgctcac cgctgcatca cgagccaccc ccaacccagc cgcagcggac ggtggcgctg ccgcccatgc cggtggctcc cctgtggata acgagatgga cgagcacgtg ccaccggtca ttgcccacgt ccgcaagaac ttcggcgtac tcgcccctac cattgagccc ttcatctacc gtagcggttg cctcaacaag tcagtggtag acgtgtacaa caacctgtcg cgcaactaca ttgccattgt agatgctgcc ctcaagcagg taggatttga agagttcacg atgcagatgc agatcatgct gacccagatc ctgtctgagg agtttggcga agatacggag tacctcgtca acaccatcct aacgtacacc ggcgccgact ccctgttcgt ctcccttctc tcgaaatcta tgctggacca ggtcatcacg tacgcgcaat caatcatgag cggcaacatg tacggcatga gctacaacat gcagagcggc ggaggcggcg gtggtcgcgg ctctgagggt gtcggtaata gttactaccg tggtgcccaa cagcatcacg gataccgtgg tgccggtgaa aacggctcat cgcgcgatct ccgcggcagc tccgtcgccg ccagccctcc tcatcaggac ggggccgcac cacagaagcc gtaccgcagc agcggcctcc caccccactc cagcgaccca ggcagggagc gcagcagcga tgatttgcgg gccaccagcg atgtaatctc acaacgcgag gagagtcggg attggggggg aggcgcttca gtgccgcctc gcggcgctac tgcgaccggg ggcatccacg tagattctag tgttggtccc cggggcggtg ccgctcccat gagcgggatg ggcgcaggtg cggccagtcg cacagaggtg tccgtgagcc gggccacgag cgagactaca tctggagcag gagtagtgcc acgtcacacg caccatcact tctcctcttc tacagcggcc gccgctcccg ccgcgaagcc gtcgtcgatg gagcgaggtg ggatgagcgg ggctccgcag cacaacctcc tacccccgcg agcttcaccg gcgagcagcc agccgcgcgt gtccacgcca ggggcgccaa gcgtgtcgca ggccaccacc acttccccga tgccgacgat gggacgcggc gggccgggca tcccgatgca ccaccatcac catcacttgg gcataggcgc aatggcaacc aacagtgccg cggccagtga ctgggccgcc agcaacagcg tatctgggtc cacaccaaac atcaacgccg ctgggcagcg caccctgacc ccaccaacgc cgcagtccgc atcgatgcag cagcatcaac atcagcacat gcagcagcaa tcccagcaac agctgccgca gccgcagcag ccgacgcgca ccatgaacat tcccgccccc ttgagtgtgc agcgccgcac agaaaagaac gcggcggcag ccgcagcagc cgctgccccg gcagcagcac ccgctaacgg cagcgcgcct gccaattcgt ctggcctcag tagtagtagc gcagctgtag agggaggggc tgcgccacgc aacatgtctg cgcctctcgg cgggagcgcg aacgtcccgg cgccgcgcgg ggcgcagtca ctcccccatc accagcacca catgcccgtc tctcaccacc atcaccatca ccatcatttc gtcgcgtccc cgcccgcggt cactgcagct gtgcagccgc cctccgctac gcactcgcca ccagcttccg aggagcgcgt tgcgaccatt cccgccgaat ccacgggcac gccggcgccg gagacgaact tacccgctaa tcaggcctct gcatcgaccc cagccccggc caggtcccct cctgccacca gcgtctcgac gacttccgca cccccgccag cgttgcgtgg cctaggccgc atccccttgc atcatcacca ccatcatatg cacttgatga tgaataaagt agatgcggcc tcggcgccgc aacggcagcc gcaaccgatt cctgcgacgt tgcagctgca gccacagccg cacctgccgc agccgcagca gcgcgtggcc accgcgatcg acgtgcatga ccagcagcag caggagcgga cgaacgccgc caacggtcgc tggcccaatc aaaaggagga actgcgaggc atgctgctga ccgcgcgcgt gtcggagagc gcggtcacgt acttgctgac ggacgtgaac atagaagagc tgcgtggctt gaagtacgta gattttgaga agaagcttcg cggtggtatt ccgctgatgt tagatcgcca gcgcatccgt caggtgctgt ccagcagcgc cgctgtgcag agtcagtcga gcgacaacga cgactccgcg acgagctcca accgaaagac tgcaaccagc ggagtaggcg cagaggagga ggaggcgggc gggtctggac tgcctcggcg acccccggcc atttccactg gcgtgcagag cccggtgacg tcgcaccagt cctcgcctat tagcaaggct ggagtgcagg tgccgcagcc tcgaacgaag atgcagcagc agcaacagca gcagacgcag caatccatca acggcggcag tgccgtggcc tcgccagcgg atgcgagctt ttcctcaccg gagaaggagt ccgacaagcg tgagggcggc accaagacgg ggagcccgac gacggccagc ggcgcaccca cctccccggt gccggcctca actccggcgc cttcccccaa cagtagctac agccctagta ctcgcaacga ggatcccgag gatagccgtg aggaggagca gcggcgtggt agcggacgcc gtggcgggaa gcgtggcggt ctggggcgcg gcggcttcag ctcatcgcgc gtgtgcaggt tcttcggtac tgcagagggg tgccagtacg gagacaagtg ccactacatg cacagcaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat