SSGCID: Target Page

LeinA.18735.a

Uncharacterized protein

CENTER ID:	LeinA.18735.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18735.a.B1.GE37551	full length		1	622

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.12.0880
RefSeq:	XP_003392344.1
UniProt:	E9AGJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAAPSGAFA QGETMEQLRL KGNACFADDP AEAAEYYTQA VQLYEERHGK DAACTMDEFT KSAGNALTCL FKMGETEQCA ALAMRVLASN PILAKANAFY GRCALVDPSL DSVSTGFGTA SAAALVHLCR AIYELPALEP STRENIDEAL TRLIGERVSV TEAFARSPEA GVKLVRGQCG VGVEAIRTLP PLVEVVSLLT PFSVGAFEDF AGRGCCVECS KVIGLDIADA AAEEPNEDEG DEENNKDDGN AQAKKEKHST CTTCDMVVYC SPACADAHRE QHERFECARM TRLSDMMEGI EARKLDVPEQ FFELAYHCIT TLAGIKAKRA GHERVLTLTA HVDEVIQSLH PIGPLMSDLF KGEEETATLY TIIGVLCCNA LEVTDPSGLG VAQALHAGNS IASFFNHSCT PNCAIDTVRH AIITTRTIHV GEELSIAYIP QLYWPTRLRR ERLSEGYYFV CRCQRCQSSN KDPFERALVM ELPTARKDAT KHFHPIVQTA CATVRSRMVD DVSQKDADEL SKLLSDLSTH LFPFHYLCHE VRNCLSFVYA VLGQTRECLC SCLDELLMWE CILPGALPVK RMKIENALEC VQGLGTKVSD CSAALLPHLS RLALVYDAEG PI

NT Sequence

atgcctgccg cgccgtctgg ggccttcgcg caaggagaaa cgatggagca gctccgcctc aagggtaacg cctgcttcgc ggatgaccct gctgaggcag ccgagtacta cacgcaggcg gtgcagctct acgaggagcg gcacgggaag gacgctgcgt gcactatgga cgagttcacc aagagcgctg gaaacgcgct tacgtgtctc tttaagatgg gggaaacgga gcagtgtgcc gcgttggcaa tgcgggttct tgcgtcgaac ccgattttag cgaaagccaa cgccttctac ggtcgctgtg ctctggtcga cccctccctg gatagcgtga gcaccggctt cggcaccgcc agtgccgcgg cgctcgtgca cctctgccgc gccatctacg agctccccgc actggagccg agcactcgcg agaacatcga cgaggcgctc actcgcctca tcggagagcg cgtaagcgtg acggaggcgt tcgcgcggtc accggaagcc ggggtgaagc tggttcgcgg gcagtgcggt gttggagtgg aggccatccg caccttgcct cctcttgtgg aggtggtgag cctgctcacc cctttctccg tcggcgcctt cgaggatttt gccggaaggg gctgctgcgt ggagtgcagt aaggttatcg gcctcgacat cgctgacgct gcggcagagg agccgaatga ggacgaaggt gacgaggaga acaacaagga tgatggcaac gcccaggcaa agaaagagaa gcactcgaca tgcaccacat gcgacatggt ggtctactgc tcgcctgcgt gcgccgatgc acaccgtgag cagcacgaga ggttcgagtg cgcacggatg acgcggctgt cggatatgat ggagggtatt gaagcccgca agctcgatgt gccggagcag ttctttgagc tggcgtacca ctgcatcaca actctggctg ggataaaggc gaagcgtgcg ggccacgaac gcgtcctcac cctgaccgca cacgtcgatg aggtgattca gtcgctgcac ccgatcggtc ccctcatgag cgacttgttc aaaggggaag aagagaccgc cacgctatac acgatcattg gcgtgctatg ctgcaacgca ctggaggtga cggacccgag cgggctcggc gtggcgcagg cgctgcacgc tggcaacagc atcgcttcat tcttcaacca ctcctgcacg ccaaattgcg cgattgacac ggtgcggcac gccatcatca ccactcgcac catccacgtc ggcgaggagc tgagcatcgc ctacatcccg cagctttact ggccaacccg tctgcgccgc gagcgtctca gcgagggcta ctactttgtg tgccggtgcc agcgatgcca gagcagcaac aaggatccgt ttgagcgtgc gctcgtcatg gagctgccta cggcacggaa agacgctaca aagcactttc acccgattgt gcagacggcc tgcgcgacgg tccgctcacg catggtggat gacgtgtcgc agaaggacgc cgacgagctc agcaagctgc tgagtgactt gtcgacgcac ctgttccctt tccattatct ctgccatgag gtgcgcaact gcctcagttt tgtgtacgcg gtgctgggcc agacgcggga gtgcctctgc agctgcctgg acgaactttt gatgtgggag tgcatcctac ccggtgctct cccagtaaag aggatgaaga ttgagaacgc gttggaatgc gtgcagggtt tgggtacgaa ggtaagtgat tgctctgcag cactgctccc tcatctgtcg cgcttggcgc tagtgtacga cgccgagggt cccatttga

Details for LeinA.18735.a.B1.GE37551

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	69kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	54

Validated AA Sequence

Validated NT Sequence

cccctannan aattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatgcc tgccgcgccg tctggggcct tcgcgcaagg agaaacgatg gagcagctcc gcctcaaggg taacgcctgc ttcgcggatg accctgctga ggcagccgag tactacacgc aggcggtgca gctctacgag gagcggcacg ggaaggacgc tgcgtgcact atggacgagt tcaccaagag cgctggaaac gcgcttacgt gtctctttaa gatgggggaa acggagcagt gtgccgcgtt ggcaatgcgg gttcttgcgt cgaacccgat tttagcgaaa gccaacgcct tctacggtcg ctgtgctctg gtcgacccct ccctggatag cgtgagcacc ggcttcggca ccgccagtgc cgcggcgctc gtgcacctct gccgcgccat ctacgagctc cccgcactgg agccgagcac tcgcgagaac atcgacgagg cgctcactcg cctcatcgga gagcgcgtaa gcgtgacgga ggcgttcgcg cggtcaccgg aagccggggt gaagctggtt cgcgggcagt gcggtgttgg agtggaggcc atccgcacct tgcctcctct tgtggaggtg gtgagcctgc tcaccccttt ctccgtcggc gccttcgagg attttgccgg aaggggctgc tgcgtggagt gcagtaaggt tatcggcctc gacatcgctg acgctgcggc agaggagccg aatgaggacg aaggtgacga ggagaacaac aaggatgatg gcaacgccca ggcaaagaaa gagaagcact cgacatgcac cacatgcaac atggtggtct actgctcgcc tgcgtgcgcc gatgcacacc gtgagcagca cgagaggttc gagtgcgcac ggatgacgcg gctgtcggat atgatggagg gtattgaagc ccgcaagctc gatgtgccgg agcagttctt tgagctggcg taccactgca tcacaactct ggctgggnta angcgancgt gcgggccacg angcgtcnca ccctgaccgc gcacgtcgat naggtgaatc agtcgctgca cccgatcgnn ccntcatgga ncgactnnna angggnnnaa nanaccnccn ngctatnnnc gatcntngcc nncnntgctn cannnnnnnn nangnncnnn nnggnnncgg nnnggncnng nnnnnnnnnc c

Expected Protein Sequence

MAHHHHHHMP AAPSGAFAQG ETMEQLRLKG NACFADDPAE AAEYYTQAVQ LYEERHGKDA ACTMDEFTKS AGNALTCLFK MGETEQCAAL AMRVLASNPI LAKANAFYGR CALVDPSLDS VSTGFGTASA AALVHLCRAI YELPALEPST RENIDEALTR LIGERVSVTE AFARSPEAGV KLVRGQCGVG VEAIRTLPPL VEVVSLLTPF SVGAFEDFAG RGCCVECSKV IGLDIADAAA EEPNEDEGDE ENNKDDGNAQ AKKEKHSTCT TCDMVVYCSP ACADAHREQH ERFECARMTR LSDMMEGIEA RKLDVPEQFF ELAYHCITTL AGIKAKRAGH ERVLTLTAHV DEVIQSLHPI GPLMSDLFKG EEETATLYTI IGVLCCNALE VTDPSGLGVA QALHAGNSIA SFFNHSCTPN CAIDTVRHAI ITTRTIHVGE ELSIAYIPQL YWPTRLRRER LSEGYYFVCR CQRCQSSNKD PFERALVMEL PTARKDATKH FHPIVQTACA TVRSRMVDDV SQKDADELSK LLSDLSTHLF PFHYLCHEVR NCLSFVYAVL GQTRECLCSC LDELLMWECI LPGALPVKRM KIENALECVQ GLGTKVSDCS AALLPHLSRL ALVYDAEGPI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg ccgcgccgtc tggggccttc gcgcaaggag aaacgatgga gcagctccgc ctcaagggta acgcctgctt cgcggatgac cctgctgagg cagccgagta ctacacgcag gcggtgcagc tctacgagga gcggcacggg aaggacgctg cgtgcactat ggacgagttc accaagagcg ctggaaacgc gcttacgtgt ctctttaaga tgggggaaac ggagcagtgt gccgcgttgg caatgcgggt tcttgcgtcg aacccgattt tagcgaaagc caacgccttc tacggtcgct gtgctctggt cgacccctcc ctggatagcg tgagcaccgg cttcggcacc gccagtgccg cggcgctcgt gcacctctgc cgcgccatct acgagctccc cgcactggag ccgagcactc gcgagaacat cgacgaggcg ctcactcgcc tcatcggaga gcgcgtaagc gtgacggagg cgttcgcgcg gtcaccggaa gccggggtga agctggttcg cgggcagtgc ggtgttggag tggaggccat ccgcaccttg cctcctcttg tggaggtggt gagcctgctc acccctttct ccgtcggcgc cttcgaggat tttgccggaa ggggctgctg cgtggagtgc agtaaggtta tcggcctcga catcgctgac gctgcggcag aggagccgaa tgaggacgaa ggtgacgagg agaacaacaa ggatgatggc aacgcccagg caaagaaaga gaagcactcg acatgcacca catgcgacat ggtggtctac tgctcgcctg cgtgcgccga tgcacaccgt gagcagcacg agaggttcga gtgcgcacgg atgacgcggc tgtcggatat gatggagggt attgaagccc gcaagctcga tgtgccggag cagttctttg agctggcgta ccactgcatc acaactctgg ctgggataaa ggcgaagcgt gcgggccacg aacgcgtcct caccctgacc gcacacgtcg atgaggtgat tcagtcgctg cacccgatcg gtcccctcat gagcgacttg ttcaaagggg aagaagagac cgccacgcta tacacgatca ttggcgtgct atgctgcaac gcactggagg tgacggaccc gagcgggctc ggcgtggcgc aggcgctgca cgctggcaac agcatcgctt cattcttcaa ccactcctgc acgccaaatt gcgcgattga cacggtgcgg cacgccatca tcaccactcg caccatccac gtcggcgagg agctgagcat cgcctacatc ccgcagcttt actggccaac ccgtctgcgc cgcgagcgtc tcagcgaggg ctactacttt gtgtgccggt gccagcgatg ccagagcagc aacaaggatc cgtttgagcg tgcgctcgtc atggagctgc ctacggcacg gaaagacgct acaaagcact ttcacccgat tgtgcagacg gcctgcgcga cggtccgctc acgcatggtg gatgacgtgt cgcagaagga cgccgacgag ctcagcaagc tgctgagtga cttgtcgacg cacctgttcc ctttccatta tctctgccat gaggtgcgca actgcctcag ttttgtgtac gcggtgctgg gccagacgcg ggagtgcctc tgcagctgcc tggacgaact tttgatgtgg gagtgcatcc tacccggtgc tctcccagta aagaggatga agattgagaa cgcgttggaa tgcgtgcagg gtttgggtac gaaggtaagt gattgctctg cagcactgct ccctcatctg tcgcgcttgg cgctagtgta cgacgccgag ggtcccattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat