LeinA.18725.a

Uncharacterized protein

CENTER ID: LeinA.18725.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18725.a.B1.GE37544 full length 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18725.a.B1.PS02015 full length 1 401 unavailable

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LinJ.30.3510
RefSeq: XP_001467205.1
UniProt: A4I636

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAALAALEKE VADLKRVRVF NETVRTGVER VLASLPSPEN EKDTSTKARI LLLRSQATLL LPRVSREAEK DLNAALKLQP GSTTTWVELS ECLLRRNAFK EACDALDNAL RVNPAHTEAL CKYSQIQRNR CGEEGVTAEQ RKAYLEDAVA KARAAVGSNV DDADAWNTLA LSLLSKVTLE GMTFDGVRKA LAAMQQAERK CPEDPDVPYN KAVLESLLGH FGAAAMDYWK AHALDKDRLK GTRHLSEENA KVLLRAQSRM RTSSGIGKRD FQRIRTRIEQ AHKKYTQSTS AKVVGVLDIV TEPTMQPVTL LVSDGKGDFG LLLLHEVRPT CFKIGDVIAY PVTTPVETIT HNVVACPGVV EEPLKLTLNH AYPNPKGILV NGQPLPSSAH VPLQMSSRLF A
NT Sequence
atggcagctc ttgccgcgct ggagaaggag gtggcagacc tgaagcgggt tcgcgtcttc aatgagacgg tgcgcaccgg tgtcgagcgc gtcctggcaa gccttccgtc acctgagaat gaaaaggata catccaccaa agctcgaatt ctactacttc gcagccaagc gaccctcttg cttccacgcg tttcgcggga ggcggagaag gatcttaatg cggctctgaa gctgcagcct ggctcgacaa cgacgtgggt cgagctcagc gagtgcctgc tccgtcgcaa tgcctttaag gaggcttgcg acgcactgga taatgctctg cgcgtaaacc cggcgcatac cgaggcgttg tgcaagtaca gccaaatcca gcgtaaccgc tgcggtgagg aaggggtcac ggctgagcag cgcaaggcgt acttggagga cgccgtggcc aaggcccgtg cagccgtagg ctccaacgtg gacgacgcag acgcatggaa cacactggcg ctgtcgctgc tgtcaaaggt gacgctggag ggcatgacgt tcgacggcgt gcgcaaggca ctggcggcga tgcagcaggc agagcggaag tgccccgagg accctgatgt tccgtacaac aaggccgtac tggaaagcct tctggggcac ttcggggccg cagcgatgga ctactggaag gcgcacgccc tcgacaagga ccgcctcaag ggcacccgcc acctctcgga ggaaaacgcg aaggtgctgc tgcgtgcgca gagccgcatg aggacgtcca gcggcattgg caagcgtgac tttcagcgga tccgcacccg catcgagcag gcccacaaaa agtacacgca gagcacctcc gccaaggtcg ttggcgtatt agacatagtc acggagccaa cgatgcaacc cgtcacgctg ctggtgtccg atggcaaggg tgactttggg cttttgctct tgcacgaggt gcgccctacg tgtttcaaga ttggcgacgt cattgcctat cccgtgacga ctcctgtcga aaccatcact cacaacgttg ttgcctgccc gggggtggtg gaggaaccgt tgaagctcac actgaaccat gcgtatccga atcccaaggg catactagtg aatggtcaac cactgccctc ctctgcgcat gtgccgctgc agatgtcgtc tcgcctcttc gcgtga
Details for LeinA.18725.a.B1.GE37544
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ALAALEKEVA DLKRVRVFNE TVRTGVERVL ASLPSPENEK DTSTKARILL LRSQATLLLP RVSREAEKDL NAALKLQPGS TTTWVELSEC LLRRNAFKEA CDALDNALRV NPAHTEALCK YSQIQRNRCG EEGVTAEQRK AYLEDAVAKA RAAVGSNVDD ADAWNTLALS LLSKVTLEGM TFDGVRKALA AMQQAERKCP EDPDVPYNKA VLESLLGHFG AAAMDYWKAH ALDKDRLKGT RHLSEENAKV LLRAQSRMRT SSGIGKRDFQ RIRTRIEQAH KKYTQSTSAK VVGVLDIVTE PTMQPVTLLV SDGKGDFGLL LLHEVRPTCF KIGDVIAYPV TTPVETITHN VVACPGVVEE PLKLTLNHAY PNPKGILVNG QPLPSSAHVP LQMSSRLFA
Validated NT Sequence
atggctcacc accaccacca ccatatggca gctcttgccg cgctggagaa ggaggtggcc gacctgaagc gggttcgcgt cttcaatgaa acagtgcgca ccggcgtcga gcgcgttttg gcaagccttc cgtcacctga gaatgaaaag gatacatcca ccaaagctcg aattctacta cttcgcagcc aagcgaccct cttgcttcca cgcgtttcgc gggaggcgga gaaggatctt aatgcggctc tgaagctgca gcctggctcg acaacgacgt gggtcgagct cagcgagtgc ctgctccgtc gcaatgcctt taaggaggct tgcgacgcac tggataatgc tctgcgcgta aacccggcgc ataccgaggc gttgtgcaag tacagccaaa tccagcgtaa ccgctgcggt gaggaagggg tcacggctga gcagcgcaag gcgtacttgg aggacgccgt ggccaaggcc cgtgcagccg taggctccaa cgtggacgac gcagacgcat ggaacacact ggcgctgtcg ctgctgtcaa aggtgacgct ggagggcatg acgttcgacg gcgtgcgcaa ggcactggcg gcgatgcagc aggcagagcg gaagtgcccc gaggaccctg atgttccgta caacaaggcc gtactggaaa gccttctggg gcacttcggg gccgcagcga tggactactg gaaggcgcac gccctcgaca aggaccgcct caagggcacc cgccacctct cggaggaaaa cgcgaaggtg ctgctgcgtg cgcagagccg catgaggacg tccagcggca ttggcaagcg tgactttcag cggatccgca cccgcatcga gcaggcccac aaaaagtaca cgcagagcac ctccgccaag gtcgttggcg tattagacat agtcacggag ccaacgatgc aacccgtcac gctgctggtg tccgatggca agggtgactt tgggcttttg ctcttgcacg aggtgcgccc tacgtgtttc aagattggcg acgtcattgc ctatcccgtg acgactcctg tcgaaaccat cactcacaac gttgttgcct gcccgggggt ggtggaggaa ccgttgaagc tcacactgaa ccatgcgtat ccgaatccca agggcatact agtgaatggt caaccactgc cctcctctgc gcatgtgccg ctgcagatgt cgtctcgcct cttcgcgtaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMA ALAALEKEVA DLKRVRVFNE TVRTGVERVL ASLPSPENEK DTSTKARILL LRSQATLLLP RVSREAEKDL NAALKLQPGS TTTWVELSEC LLRRNAFKEA CDALDNALRV NPAHTEALCK YSQIQRNRCG EEGVTAEQRK AYLEDAVAKA RAAVGSNVDD ADAWNTLALS LLSKVTLEGM TFDGVRKALA AMQQAERKCP EDPDVPYNKA VLESLLGHFG AAAMDYWKAH ALDKDRLKGT RHLSEENAKV LLRAQSRMRT SSGIGKRDFQ RIRTRIEQAH KKYTQSTSAK VVGVLDIVTE PTMQPVTLLV SDGKGDFGLL LLHEVRPTCF KIGDVIAYPV TTPVETITHN VVACPGVVEE PLKLTLNHAY PNPKGILVNG QPLPSSAHVP LQMSSRLFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag ctcttgccgc gctggagaag gaggtggcag acctgaagcg ggttcgcgtc ttcaatgaga cggtgcgcac cggtgtcgag cgcgtcctgg caagccttcc gtcacctgag aatgaaaagg atacatccac caaagctcga attctactac ttcgcagcca agcgaccctc ttgcttccac gcgtttcgcg ggaggcggag aaggatctta atgcggctct gaagctgcag cctggctcga caacgacgtg ggtcgagctc agcgagtgcc tgctccgtcg caatgccttt aaggaggctt gcgacgcact ggataatgct ctgcgcgtaa acccggcgca taccgaggcg ttgtgcaagt acagccaaat ccagcgtaac cgctgcggtg aggaaggggt cacggctgag cagcgcaagg cgtacttgga ggacgccgtg gccaaggccc gtgcagccgt aggctccaac gtggacgacg cagacgcatg gaacacactg gcgctgtcgc tgctgtcaaa ggtgacgctg gagggcatga cgttcgacgg cgtgcgcaag gcactggcgg cgatgcagca ggcagagcgg aagtgccccg aggaccctga tgttccgtac aacaaggccg tactggaaag ccttctgggg cacttcgggg ccgcagcgat ggactactgg aaggcgcacg ccctcgacaa ggaccgcctc aagggcaccc gccacctctc ggaggaaaac gcgaaggtgc tgctgcgtgc gcagagccgc atgaggacgt ccagcggcat tggcaagcgt gactttcagc ggatccgcac ccgcatcgag caggcccaca aaaagtacac gcagagcacc tccgccaagg tcgttggcgt attagacata gtcacggagc caacgatgca acccgtcacg ctgctggtgt ccgatggcaa gggtgacttt gggcttttgc tcttgcacga ggtgcgccct acgtgtttca agattggcga cgtcattgcc tatcccgtga cgactcctgt cgaaaccatc actcacaacg ttgttgcctg cccgggggtg gtggaggaac cgttgaagct cacactgaac catgcgtatc cgaatcccaa gggcatacta gtgaatggtc aaccactgcc ctcctctgcg catgtgccgc tgcagatgtc gtctcgcctc ttcgcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for LeinA.18725.a.B1.PS02015
PURIFICATION DATe: 3/6/2014
CONCENTRATION: 27.5mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA ALAALEKEVA DLKRVRVFNE TVRTGVERVL ASLPSPENEK DTSTKARILL LRSQATLLLP RVSREAEKDL NAALKLQPGS TTTWVELSEC LLRRNAFKEA CDALDNALRV NPAHTEALCK YSQIQRNRCG EEGVTAEQRK AYLEDAVAKA RAAVGSNVDD ADAWNTLALS LLSKVTLEGM TFDGVRKALA AMQQAERKCP EDPDVPYNKA VLESLLGHFG AAAMDYWKAH ALDKDRLKGT RHLSEENAKV LLRAQSRMRT SSGIGKRDFQ RIRTRIEQAH KKYTQSTSAK VVGVLDIVTE PTMQPVTLLV SDGKGDFGLL LLHEVRPTCF KIGDVIAYPV TTPVETITHN VVACPGVVEE PLKLTLNHAY PNPKGILVNG QPLPSSAHVP LQMSSRLFA
Validated NT Sequence
atggctcacc accaccacca ccatatggca gctcttgccg cgctggagaa ggaggtggcc gacctgaagc gggttcgcgt cttcaatgaa acagtgcgca ccggcgtcga gcgcgttttg gcaagccttc cgtcacctga gaatgaaaag gatacatcca ccaaagctcg aattctacta cttcgcagcc aagcgaccct cttgcttcca cgcgtttcgc gggaggcgga gaaggatctt aatgcggctc tgaagctgca gcctggctcg acaacgacgt gggtcgagct cagcgagtgc ctgctccgtc gcaatgcctt taaggaggct tgcgacgcac tggataatgc tctgcgcgta aacccggcgc ataccgaggc gttgtgcaag tacagccaaa tccagcgtaa ccgctgcggt gaggaagggg tcacggctga gcagcgcaag gcgtacttgg aggacgccgt ggccaaggcc cgtgcagccg taggctccaa cgtggacgac gcagacgcat ggaacacact ggcgctgtcg ctgctgtcaa aggtgacgct ggagggcatg acgttcgacg gcgtgcgcaa ggcactggcg gcgatgcagc aggcagagcg gaagtgcccc gaggaccctg atgttccgta caacaaggcc gtactggaaa gccttctggg gcacttcggg gccgcagcga tggactactg gaaggcgcac gccctcgaca aggaccgcct caagggcacc cgccacctct cggaggaaaa cgcgaaggtg ctgctgcgtg cgcagagccg catgaggacg tccagcggca ttggcaagcg tgactttcag cggatccgca cccgcatcga gcaggcccac aaaaagtaca cgcagagcac ctccgccaag gtcgttggcg tattagacat agtcacggag ccaacgatgc aacccgtcac gctgctggtg tccgatggca agggtgactt tgggcttttg ctcttgcacg aggtgcgccc tacgtgtttc aagattggcg acgtcattgc ctatcccgtg acgactcctg tcgaaaccat cactcacaac gttgttgcct gcccgggggt ggtggaggaa ccgttgaagc tcacactgaa ccatgcgtat ccgaatccca agggcatact agtgaatggt caaccactgc cctcctctgc gcatgtgccg ctgcagatgt cgtctcgcct cttcgcgtaa gtgagtaaga t
Expressed Protein Sequence
MAHHHHHHMA ALAALEKEVA DLKRVRVFNE TVRTGVERVL ASLPSPENEK DTSTKARILL LRSQATLLLP RVSREAEKDL NAALKLQPGS TTTWVELSEC LLRRNAFKEA CDALDNALRV NPAHTEALCK YSQIQRNRCG EEGVTAEQRK AYLEDAVAKA RAAVGSNVDD ADAWNTLALS LLSKVTLEGM TFDGVRKALA AMQQAERKCP EDPDVPYNKA VLESLLGHFG AAAMDYWKAH ALDKDRLKGT RHLSEENAKV LLRAQSRMRT SSGIGKRDFQ RIRTRIEQAH KKYTQSTSAK VVGVLDIVTE PTMQPVTLLV SDGKGDFGLL LLHEVRPTCF KIGDVIAYPV TTPVETITHN VVACPGVVEE PLKLTLNHAY PNPKGILVNG QPLPSSAHVP LQMSSRLFA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG CTCTTGCCGC GCTGGAGAAG GAGGTGGCAG ACCTGAAGCG GGTTCGCGTC TTCAATGAGA CGGTGCGCAC CGGTGTCGAG CGCGTCCTGG CAAGCCTTCC GTCACCTGAG AATGAAAAGG ATACATCCAC CAAAGCTCGA ATTCTACTAC TTCGCAGCCA AGCGACCCTC TTGCTTCCAC GCGTTTCGCG GGAGGCGGAG AAGGATCTTA ATGCGGCTCT GAAGCTGCAG CCTGGCTCGA CAACGACGTG GGTCGAGCTC AGCGAGTGCC TGCTCCGTCG CAATGCCTTT AAGGAGGCTT GCGACGCACT GGATAATGCT CTGCGCGTAA ACCCGGCGCA TACCGAGGCG TTGTGCAAGT ACAGCCAAAT CCAGCGTAAC CGCTGCGGTG AGGAAGGGGT CACGGCTGAG CAGCGCAAGG CGTACTTGGA GGACGCCGTG GCCAAGGCCC GTGCAGCCGT AGGCTCCAAC GTGGACGACG CAGACGCATG GAACACACTG GCGCTGTCGC TGCTGTCAAA GGTGACGCTG GAGGGCATGA CGTTCGACGG CGTGCGCAAG GCACTGGCGG CGATGCAGCA GGCAGAGCGG AAGTGCCCCG AGGACCCTGA TGTTCCGTAC AACAAGGCCG TACTGGAAAG CCTTCTGGGG CACTTCGGGG CCGCAGCGAT GGACTACTGG AAGGCGCACG CCCTCGACAA GGACCGCCTC AAGGGCACCC GCCACCTCTC GGAGGAAAAC GCGAAGGTGC TGCTGCGTGC GCAGAGCCGC ATGAGGACGT CCAGCGGCAT TGGCAAGCGT GACTTTCAGC GGATCCGCAC CCGCATCGAG CAGGCCCACA AAAAGTACAC GCAGAGCACC TCCGCCAAGG TCGTTGGCGT ATTAGACATA GTCACGGAGC CAACGATGCA ACCCGTCACG CTGCTGGTGT CCGATGGCAA GGGTGACTTT GGGCTTTTGC TCTTGCACGA GGTGCGCCCT ACGTGTTTCA AGATTGGCGA CGTCATTGCC TATCCCGTGA CGACTCCTGT CGAAACCATC ACTCACAACG TTGTTGCCTG CCCGGGGGTG GTGGAGGAAC CGTTGAAGCT CACACTGAAC CATGCGTATC CGAATCCCAA GGGCATACTA GTGAATGGTC AACCACTGCC CTCCTCTGCG CATGTGCCGC TGCAGATGTC GTCTCGCCTC TTCGCGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T