LeinA.18722.a

Uncharacterized protein

CENTER ID: LeinA.18722.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18722.a.B1.GE37542 full length 1 730
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LinJ.30.0680
RefSeq: XP_001466913.1
UniProt: A4I594

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGCLNSKSTA EKTTSGPKRF GYHPKSNNKH HNEEKGVVSK YSAVKGWAGS SDNDERTEEV GPDANKATKA SAERFLRSIL EGAELFFSGL PDSQAVKQLI ALTFTNSCAE VTRTKFRLKL DLITVLLTSL ARQLKLPLIE ASVPQVEERA MDDGFSRILL AWFSNNPSFG KTIPVTSGQA VLNTLYIRYV VSKSVSFEDI LAYQFRWVSA NDAATEVFNK FTDGKESMTS EQLGKFLRDV QRSEVTDRQI VEKFKYCFGG GIHKYNFISY NGSVLTNNAI DPARTSDVWQ DMTQSFTHYM VSCARIETEE DLKRAAADGT RALVLNINKA SNGKLMVGSC ELQTILEGVK KNGFATNTYP IVLCLSPGTP MPTPVQDEVA KMINDILESA VAEGLMFEGA MINDPKFSPG ALRNKVLVMS HQSRLKPFIG FLVADMNKDG LGVRVTDVME GTPASKGGVL KDDWLTHING IPIQNKQHLR ETLARLKVGD EFTVRRENLH DVKVVVGGAI DPQDTTASAS LSSITFFKYS DSNDPKPWDT ERVPASSLAT TKLTRKDLLT HFAFCTVNSA EVSTELPDAE GKAASLGIQF VDVDNSERCL PWTRGRFSDN GRCGYILKTD IDSAKSPELS FSVIGGPRLL NCSPLTSMNV TIHGAGAARV NGSQVSFTDC NQATIAVVQM TFEANGAERT FTSAFCPGLL RPGYRALPSV PFGEERASKK QIHGIYCFIK
NT Sequence
atggggtgcc taaactccaa atcaacggca gaaaagacga cctcgggccc gaagaggttt ggctatcacc ccaaaagtaa caacaagcat cacaatgaag agaagggggt tgtctcgaaa tacagcgcag tcaaggggtg ggcaggttct tccgacaatg atgagaggac tgaagaggtg gggccagatg cgaacaaagc aacaaaggcc tccgctgaac gtttcttgcg ctctattctg gagggtgcgg agctcttctt cagtggcctg cccgattcgc aggcggtgaa gcaactaatt gcgttgacgt ttaccaattc ctgtgccgag gtaacacgca ccaagttccg cctcaagctt gacctcatca cggttcttct gacgtcatta gcgcggcagc tcaagttgcc gttgatagag gcatccgtgc cgcaggttga ggagcgtgcc atggacgacg gcttctcgcg catactgcta gcgtggtttt cgaacaaccc gtcgttcgga aagacgattc cggtgacctc ggggcaggcg gtgctcaata ccctgtacat tcggtacgtg gtgagtaaat ctgtttcgtt cgaggacatc ctagcctatc aattccgctg ggtgtctgca aacgacgccg cgacggaagt gttcaacaag ttcacggacg ggaaagagtc gatgacatca gagcaactag gcaagttcct gcgtgatgtg cagcgcagcg aggtcacaga tcgccagatt gtggagaagt tcaagtattg ctttggcggc ggcatccaca agtataactt catcagctac aatgggagcg tattgacaaa caacgccatc gaccctgcac ggacgtctga cgtgtggcag gacatgacgc aatccttcac gcactacatg gtctcgtgtg cgcgcatcga aactgaggaa gacctcaagc gtgccgcagc ggacggaacg cgcgctttgg tgctcaacat caataaagcg agcaacggaa agctcatggt gggatcttgc gagttgcaga ccatcttgga gggcgtaaag aagaacggct ttgcgacgaa cacgtacccg atcgtccttt gtctttctcc tggcacaccg atgcccactc ctgttcagga tgaggtggca aagatgatca acgacattct ggagtccgcc gtggcggagg gtctcatgtt tgagggtgcc atgatcaatg accccaagtt cagccccggt gcgttgcgca acaaggtgct tgttatgagc catcagagtc gcctgaagcc cttcattggc tttctggtgg ccgatatgaa caaggatggc ctcggcgtcc gcgtcacgga tgtgatggag gggacgccgg cgtcgaaggg tggcgtgctc aaggatgatt ggctgactca catcaacggt attccaatcc agaacaagca gcatttgcga gaaaccctgg cgcgtctcaa ggtcggcgat gagttcactg tgcgccgaga aaacttgcat gatgtcaagg tggtggtggg cggcgccatt gatccgcagg acaccactgc gtccgcgtct ctctctagca tcactttctt caagtactct gacagtaatg atccgaagcc gtgggacacg gagcgcgtcc ccgcatcgag cctcgccacg actaagttga cacggaagga tttgctgacg catttcgcgt tctgcacagt gaactccgct gaagtgtcca cggagctgcc agatgcggag ggcaaggcgg ccagcctggg tatccagttt gtcgacgttg acaacagcga gcggtgcctt ccctggactc gtggacgatt ctctgacaat ggtcgctgcg ggtacattct taaaaccgac atcgactccg ccaagtctcc tgagctgagc ttcagcgtta tcggtggccc tcgcctcctc aactgctcgc cgttgacgtc gatgaatgtc actatccatg gcgcaggcgc ggcgcgcgtg aatgggagtc aggtttcctt cacggattgc aaccaggcta caatagcagt ggtgcagatg acgtttgaag cgaacggcgc ggagcggacc tttacctcgg ctttttgccc tggtcttctt cgccctggtt accgcgccct cccttccgtc ccctttggcg aagagcgcgc ctcgaagaag cagatccatg gcatctactg cttcatcaag tag
Details for LeinA.18722.a.B1.GE37542
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 81kDa
OBSERVED MW: 81kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 39
Validated AA Sequence
MGCLNSKSTA EKTTSGPKRF GYHPKSNNKH HNEEKGVVSK YSAVKGWAGS SDNDERTEEV GPDANKATKA SAERFLRSIL EGAELFFSGL PDSQAVKQLI ALTFTNSCAE VTRTKFRLKL DLITVLLTSL ARQLKLPLIE ASVPQVEERA MDDGFSRILL AWFSNNPSFG KTIPVTSGQA VLNTLYIRYV VSKSVSFEDI LAYQFRWVSA NDAATEVFNK FTDGKESMTS EQLGKFLRDV QRSEVTDRQI VEKFKYCFGG GIHKYNFISY NGSVLTNNAI DPARTSDVWX X
Validated NT Sequence
ggctcaccac caccaccacc atatggggtg cctaaactcc aaatcaacgg cagaaaagac gacctcgggc ccgaagaggt ttggctatca ccccaaaagt aacaacaagc atcacaatga agagaagggg gttgtctcga aatacagcgc agtcaagggg tgggcaggtt cttccgacaa tgatgagagg actgaagagg tggggccaga tgcgaacaaa gcaacaaagg cctccgctga acgtttcttg cgctctattc tggagggtgc ggagctcttc ttcagtggcc tgcccgattc gcaggcggtg aagcaactaa ttgcgttgac gtttaccaat tcctgtgccg aggtaacacg caccaagttc cgcctcaagc ttgacctcat cacggttctt ctgacgtcat tagcgcggca gctcaagttg ccgttgatag aggcatccgt gccgcaggtt gaggagcgtg ccatggacga cggcttctcg cgcatactgc tagcgtggtt ttcgaacaac ccgtcgttcg gaaagacgat tccggtgacc tcggggcagg cggtgctcaa taccctgtac atccggtacg tggtgagtaa atctgtttcg ttcgaggaca tcctagccta tcaattccgc tgggtgtctg caaacgacgc cgcgacggaa gtgttcaaca agttcacgga cgggaaagag tcgatgacat cagagcaact aggcaagttc ctgcgtgatg tgcagcgcag cgaggtcaca gatcgccaga ttgtggagaa gttcaagtat tgctttggcg gcggcatcca caagtataac ttcatcagct acaatgggag cgtattgaca aacaacgcca tcgaccctgc acggacgtct gacgtgtggc nnncntgacn nnanccttca cgcactacan ggtctcgtgt gcnnnnatcg nanctgagna nacctcnngc gtgccgcanc nnanggnacg ngcgcttnng ngntcanatc
Expected Protein Sequence
MAHHHHHHMG CLNSKSTAEK TTSGPKRFGY HPKSNNKHHN EEKGVVSKYS AVKGWAGSSD NDERTEEVGP DANKATKASA ERFLRSILEG AELFFSGLPD SQAVKQLIAL TFTNSCAEVT RTKFRLKLDL ITVLLTSLAR QLKLPLIEAS VPQVEERAMD DGFSRILLAW FSNNPSFGKT IPVTSGQAVL NTLYIRYVVS KSVSFEDILA YQFRWVSAND AATEVFNKFT DGKESMTSEQ LGKFLRDVQR SEVTDRQIVE KFKYCFGGGI HKYNFISYNG SVLTNNAIDP ARTSDVWQDM TQSFTHYMVS CARIETEEDL KRAAADGTRA LVLNINKASN GKLMVGSCEL QTILEGVKKN GFATNTYPIV LCLSPGTPMP TPVQDEVAKM INDILESAVA EGLMFEGAMI NDPKFSPGAL RNKVLVMSHQ SRLKPFIGFL VADMNKDGLG VRVTDVMEGT PASKGGVLKD DWLTHINGIP IQNKQHLRET LARLKVGDEF TVRRENLHDV KVVVGGAIDP QDTTASASLS SITFFKYSDS NDPKPWDTER VPASSLATTK LTRKDLLTHF AFCTVNSAEV STELPDAEGK AASLGIQFVD VDNSERCLPW TRGRFSDNGR CGYILKTDID SAKSPELSFS VIGGPRLLNC SPLTSMNVTI HGAGAARVNG SQVSFTDCNQ ATIAVVQMTF EANGAERTFT SAFCPGLLRP GYRALPSVPF GEERASKKQI HGIYCFIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggt gcctaaactc caaatcaacg gcagaaaaga cgacctcggg cccgaagagg tttggctatc accccaaaag taacaacaag catcacaatg aagagaaggg ggttgtctcg aaatacagcg cagtcaaggg gtgggcaggt tcttccgaca atgatgagag gactgaagag gtggggccag atgcgaacaa agcaacaaag gcctccgctg aacgtttctt gcgctctatt ctggagggtg cggagctctt cttcagtggc ctgcccgatt cgcaggcggt gaagcaacta attgcgttga cgtttaccaa ttcctgtgcc gaggtaacac gcaccaagtt ccgcctcaag cttgacctca tcacggttct tctgacgtca ttagcgcggc agctcaagtt gccgttgata gaggcatccg tgccgcaggt tgaggagcgt gccatggacg acggcttctc gcgcatactg ctagcgtggt tttcgaacaa cccgtcgttc ggaaagacga ttccggtgac ctcggggcag gcggtgctca ataccctgta cattcggtac gtggtgagta aatctgtttc gttcgaggac atcctagcct atcaattccg ctgggtgtct gcaaacgacg ccgcgacgga agtgttcaac aagttcacgg acgggaaaga gtcgatgaca tcagagcaac taggcaagtt cctgcgtgat gtgcagcgca gcgaggtcac agatcgccag attgtggaga agttcaagta ttgctttggc ggcggcatcc acaagtataa cttcatcagc tacaatggga gcgtattgac aaacaacgcc atcgaccctg cacggacgtc tgacgtgtgg caggacatga cgcaatcctt cacgcactac atggtctcgt gtgcgcgcat cgaaactgag gaagacctca agcgtgccgc agcggacgga acgcgcgctt tggtgctcaa catcaataaa gcgagcaacg gaaagctcat ggtgggatct tgcgagttgc agaccatctt ggagggcgta aagaagaacg gctttgcgac gaacacgtac ccgatcgtcc tttgtctttc tcctggcaca ccgatgccca ctcctgttca ggatgaggtg gcaaagatga tcaacgacat tctggagtcc gccgtggcgg agggtctcat gtttgagggt gccatgatca atgaccccaa gttcagcccc ggtgcgttgc gcaacaaggt gcttgttatg agccatcaga gtcgcctgaa gcccttcatt ggctttctgg tggccgatat gaacaaggat ggcctcggcg tccgcgtcac ggatgtgatg gaggggacgc cggcgtcgaa gggtggcgtg ctcaaggatg attggctgac tcacatcaac ggtattccaa tccagaacaa gcagcatttg cgagaaaccc tggcgcgtct caaggtcggc gatgagttca ctgtgcgccg agaaaacttg catgatgtca aggtggtggt gggcggcgcc attgatccgc aggacaccac tgcgtccgcg tctctctcta gcatcacttt cttcaagtac tctgacagta atgatccgaa gccgtgggac acggagcgcg tccccgcatc gagcctcgcc acgactaagt tgacacggaa ggatttgctg acgcatttcg cgttctgcac agtgaactcc gctgaagtgt ccacggagct gccagatgcg gagggcaagg cggccagcct gggtatccag tttgtcgacg ttgacaacag cgagcggtgc cttccctgga ctcgtggacg attctctgac aatggtcgct gcgggtacat tcttaaaacc gacatcgact ccgccaagtc tcctgagctg agcttcagcg ttatcggtgg ccctcgcctc ctcaactgct cgccgttgac gtcgatgaat gtcactatcc atggcgcagg cgcggcgcgc gtgaatggga gtcaggtttc cttcacggat tgcaaccagg ctacaatagc agtggtgcag atgacgtttg aagcgaacgg cgcggagcgg acctttacct cggctttttg ccctggtctt cttcgccctg gttaccgcgc cctcccttcc gtcccctttg gcgaagagcg cgcctcgaag aagcagatcc atggcatcta ctgcttcatc aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat