LeinA.18721.a

Uncharacterized protein

CENTER ID: LeinA.18721.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18721.a.B1.GE37541 full length 1 308
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LinJ.34.3930
RefSeq: XP_001468750.1
UniProt: A4IAI1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSWKDRIAA MLFFRDPEEA LTAEKMRNAE AMAKTTEVRL HHNQDEREVK EKMLQLENGI KAQRERYARQ AAPMLKEFDD IAISQHYYQE VGNSVAAQET FVDQMAQRET HQFGYISKKL ISVSLNFEAL RQQMRSGKPF ARELKATLDD AESEDLNAMS EPLRAFADRG VPESTLVRAA AFDLARSIEE TGKAPVQQPV LGWLDLLKFR TAFSPSTVDQ NEVRARRTAA QFTRYIEQRQ YARALALAEE VDTWTRNEHD AAVEYFNNSY RSFRQATLPV ITAEIFLAYA AASLNASRVA CVEHMLKE
NT Sequence
atgacgtcct ggaaggaccg gatcgccgcg atgctcttct tccgtgaccc tgaggaggcg ctaacagcgg agaagatgcg caacgccgag gcgatggcca agacaacgga ggtgcgcctg catcacaacc aagatgagcg ggaggtcaag gagaagatgc tgcagctgga aaatggcatc aaggcgcagc gggagcgcta cgctcgacag gccgccccaa tgctgaagga gttcgacgac attgccattt cgcagcacta ctaccaggag gtgggaaaca gcgtggcagc gcaggagacg tttgtggatc aaatggcgca gcgggagacg caccaattcg gctacataag caagaagctc atctccgtca gcctcaactt tgaggcttta cgccagcaga tgcggtcagg gaagccgttc gcacgagagc tgaaggccac gctcgatgat gcggagagtg aggacctgaa tgccatgtcg gagccactcc gtgcatttgc cgaccgcggc gtcccggagt cgacgcttgt gcgcgctgcc gcctttgatc ttgcccgctc catcgaggag actggcaagg caccggtgca gcagccggtg ctggggtggc tggacctgtt aaagtttcgt accgccttta gtccctctac ggtagaccag aacgaagttc gcgctcgccg caccgctgcc cagttcacgc gctacatcga gcagagacag tacgcgaggg cactggctct agcggaggag gtagacacgt ggacgcgcaa tgagcacgat gccgccgtgg aatacttcaa caattcctac aggagcttcc ggcaagcgac cctgcccgtc atcacggctg aaatctttct cgcctacgcc gccgcctccc tcaacgcgtc acgggtggcg tgtgtggaac atatgctgaa ggaataa
Details for LeinA.18721.a.B1.GE37541
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMT SWKDRIAAML FFRDPEEALT AEKMRNAEAM AKTTEVRLQH NQDEREVKEK MLQLENGIKA QRERYARQAA PMLKEFDDIA ISQHYYQEVG NSVAAQETFV DQMAQRETHQ FGYISKKLIS VSLNFEALRQ QMRSGKPFAR ELKATLDDAE SEDLNAMSEP LRAFADRGVP ESTLVRAAAF DLARSIEETG KAPVQQPVLG WLDLLKFRTA FSPSTVDQNE VRARRTAAQF TRYIEQRQYA RALALAEEVD TWTRNEHDAA VEYFNNSYRS FRQATLPVIT AEIFLAYAAA SLNASRVACV EHMLKEXVSX
Validated NT Sequence
gnntattttn tttactttaa gaaggagata taccatggct caccaccacc accaccatat gacgtcctgg aaggaccgga tcgccgcgat gctcttcttc cgtgaccctg aggaggcgct aacagcggag aagatgcgca acgccgaggc gatggccaag acaacggagg tgcgcctgca gcacaaccaa gatgagcggg aggtcaagga gaagatgctg cagctggaaa atggcatcaa ggcgcagcgg gagcgctacg ctcgacaggc cgccccaatg ctgaaggagt tcgacgacat tgccatttcg cagcactact accaggaggt gggaaacagc gtggccgcgc aggagacgtt tgtggatcaa atggcgcagc gggagacgca ccaattcggc tacataagca agaagctcat ctccgtcagc ctcaattttg aggctttacg ccagcagatg cggtcaggga agccgttcgc acgagagctg aaggccacgc tcgatgatgc ggagagtgag gacctgaatg ccatgtcgga gccactccgt gcatttgccg accgcggcgt cccggagtcg acgcttgtgc gcgctgccgc ctttgatctt gcccgctcca tcgaggagac tggcaaggca ccggtgcagc agccggtgct ggggtggctg gacctgttaa agtttcgtac cgcctttagt ccctctacgg tagaccagaa cgaagttcgc gctcgccgca ccgctgccca gttcacgcgc tacatcgagc agagacagta cgcgagggca ctggctctag cggaggaggt agacacgtgg acgcgcaatg agcacgatgc cgccgtggaa tacttcaaca attcctacag gagcttccgg caagcgaccc tgcccgtcat cacggctgaa atctttctcg cctacgccgc cgcctccctc aacgcgtcac gggtggcgtg tgtggaacat atgctgaagg aatnagtgag tanataggat cgnnnncccc ccccncnngn annnnanngg nnnannannn naaananana nannnnnggg ggnancgccc tgggcggacn aacgtcnnat gncctgttga ctnnttagat cccngggcgg anncnngncn nnnntatgcg nagantngaa ttannnnggn nnnnnnnnna nnnnnggnnn ccnnantncg ngggtnntta gcctcggnna nnncnnnnaa ncnnnngcnn nnnnnnnaaa ntngtnnnnn ncgnnnnggt ncncnacnga nnnnntnnnn catnacncnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMT SWKDRIAAML FFRDPEEALT AEKMRNAEAM AKTTEVRLHH NQDEREVKEK MLQLENGIKA QRERYARQAA PMLKEFDDIA ISQHYYQEVG NSVAAQETFV DQMAQRETHQ FGYISKKLIS VSLNFEALRQ QMRSGKPFAR ELKATLDDAE SEDLNAMSEP LRAFADRGVP ESTLVRAAAF DLARSIEETG KAPVQQPVLG WLDLLKFRTA FSPSTVDQNE VRARRTAAQF TRYIEQRQYA RALALAEEVD TWTRNEHDAA VEYFNNSYRS FRQATLPVIT AEIFLAYAAA SLNASRVACV EHMLKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt cctggaagga ccggatcgcc gcgatgctct tcttccgtga ccctgaggag gcgctaacag cggagaagat gcgcaacgcc gaggcgatgg ccaagacaac ggaggtgcgc ctgcatcaca accaagatga gcgggaggtc aaggagaaga tgctgcagct ggaaaatggc atcaaggcgc agcgggagcg ctacgctcga caggccgccc caatgctgaa ggagttcgac gacattgcca tttcgcagca ctactaccag gaggtgggaa acagcgtggc agcgcaggag acgtttgtgg atcaaatggc gcagcgggag acgcaccaat tcggctacat aagcaagaag ctcatctccg tcagcctcaa ctttgaggct ttacgccagc agatgcggtc agggaagccg ttcgcacgag agctgaaggc cacgctcgat gatgcggaga gtgaggacct gaatgccatg tcggagccac tccgtgcatt tgccgaccgc ggcgtcccgg agtcgacgct tgtgcgcgct gccgcctttg atcttgcccg ctccatcgag gagactggca aggcaccggt gcagcagccg gtgctggggt ggctggacct gttaaagttt cgtaccgcct ttagtccctc tacggtagac cagaacgaag ttcgcgctcg ccgcaccgct gcccagttca cgcgctacat cgagcagaga cagtacgcga gggcactggc tctagcggag gaggtagaca cgtggacgcg caatgagcac gatgccgccg tggaatactt caacaattcc tacaggagct tccggcaagc gaccctgccc gtcatcacgg ctgaaatctt tctcgcctac gccgccgcct ccctcaacgc gtcacgggtg gcgtgtgtgg aacatatgct gaaggaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at