LeinA.18719.a

Uncharacterized protein

CENTER ID: LeinA.18719.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18719.a.B1.GE37540 full length 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LinJ.07.1290
RefSeq: XP_001463328.1
UniProt: A4HTD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLKHVASFG AVGVLSVVGV LGGTRWRRVE LRRAELNEEY TKLMTEMRTF NEERLTRDGR LAAKEVEAKA TAETVDILWS DRLARYAEVN KDLHAYLAAL PEAIGALKGL SNHYRYMSEE MTKFMGFDIA CSKMHNLALM LEHGKAVGIE RVAAAVQEMF AAEPLVQTVC TSILAAPAPP HPSSIAAASA TFTFCMEELD RAVGKVAMRY AAALEELPNA TPGILSDSVR KLVGMTRADT LSKGQRQLAE RRRDLERMLR RAQRQLHTEE DIRAALDYTR ELDQHLQAAA PKRTDLLLSL PSRKDNFLAA VRSDSEVKKA IQQINLWRDS ATTFLVHRQA EDALQSYYLL LAETLTAVNE LK
NT Sequence
atgtcactca agcatgtggc gtcttttggc gctgttggtg tcttatcggt agtcggcgta cttggaggaa cgcgatggcg ccgcgtcgag ctgcgccgcg cggagctaaa cgaggagtac acgaagctca tgactgaaat gcgtaccttc aacgaggagc gtctcacccg cgatgggcgc ctggccgcca aggaggtgga ggcgaaggcg acggcggaga cggtggacat attatggagc gatcggctgg cgcgttatgc ggaggtgaac aaggacctgc atgcctacct ggccgccttg cctgaggcga ttggagcact gaagggtctg tcgaaccact atcgctacat gtcggaggag atgaccaagt tcatggggtt cgacatcgcg tgctccaaga tgcacaacct ggcactgatg ctggagcacg gcaaggcggt aggcattgag cgagtggcgg cggcggtgca ggagatgttt gccgcggagc cgcttgtgca gacggtgtgc acgagcatct tggcagcccc ggctccgccg catcccagtt cgatcgctgc ggccagcgcc accttcacgt tctgcatgga agagctcgac cgcgccgtgg ggaaggttgc tatgcggtat gcggcagcac tggaagagct cccaaatgcc acgcctggca tcttgtcaga cagtgtccgg aagcttgtcg gcatgacgcg agcagacacg ctcagcaagg ggcagcgcca gcttgcggag cgccgaaggg acttggaacg gatgcttcgc cgtgcccagc gccagctgca caccgaggag gacattcggg cagcgctgga ttacacgagg gagctggatc agcatcttca ggcggcggca ccgaaaagaa ccgacttgtt gctttcattg ccttccagga aggataactt tctggccgcc gtgcggtcgg acagtgaggt gaaaaaggcc atccagcaga tcaacctgtg gcgtgactcg gcaaccactt tcctggtgca ccgccaggcc gaggacgcgc tgcagagcta ctacttactc ctggccgaga cgcttacagc agtgaatgag ttgaagtag
Details for LeinA.18719.a.B1.GE37540
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMS LKHVASFGAV GVLSVVGVLG GTRWRRVELR RAELNEEYTK LMTEMRTFNE ERLTRDGRLA AKEVEAKATA ETVDILWSDR LARYAEVNKD LHAYLAALPE AIGALKGLSN HYRYMSEEMP KFMGFDIACS KMHNLALMLE HGKAVGIERV AAAVQEMFAA EPLVQTVCTS ILAAPAPPHP SSIAAASATF TFCMEELDRA VGKVAMRYAA ALEEPPNATP GILSDSVRKL VGMTRADTLS KGQRQLAERR RDLERMLRRA QRQLHTEEDI RAALDYTREL DQHLQAAAPK RTDLLLSLPS RKDNFLAAVR SDSEVKXHPA DQPVA
Validated NT Sequence
cnnnnataat tttntttact ttaagaagga gatataccat ggctcaccac caccaccacc atatgtcact caagcatgtg gcgtcttttg gcgctgttgg tgtcttatcg gtagtcggcg tacttggagg aacgcgatgg cgccgcgtcg agctgcgccg cgcggagcta aacgaggagt acacgaagct catgactgaa atgcgtacct tcaacgagga gcgtctcacc cgcgatgggc gcctggccgc caaggaggtg gaggcgaagg cgacggcgga gacggtggac atattatgga gcgatcggct ggcgcgttat gcggaggtga acaaggacct gcatgcctac ctggccgcct tgcctgaggc gattggagca ctgaagggtc tgtcgaacca ctatcgctac atgtcggagg agatgcccaa gttcatgggg ttcgacatcg cgtgctccaa gatgcacaac ctggcactga tgctggagca cggcaaggcg gtaggcattg agcgagtggc ggcggcggtg caggagatgt ttgccgcgga gccgcttgtg cagacggtgt gcacgagcat cttggcagcc ccggctccgc cgcatcccag ttcgatcgct gcggccagcg ccaccttcac gttctgcatg gaagagctcg accgcgccgt ggggaaggtt gctatgcggt atgcggcagc actggaagag cccccaaatg ccacgcctgg catcttgtca gacagtgtcc ggaagcttgt cggcatgacg cgagcagaca cgctcagcaa ggggcagcgc cagcttgcgg agcgccgaag ggacttggaa cggatgcttc gccgtgccca gcgccagctg cacaccgagg aggacattcg ggcagcgctg gattacacga gggagctgga tcagcatctt caggcggcgg caccgaaaag aaccgacttg ttgctttcat tgccttctag gaaggataac tttctggccg ccgtgcggtc ggacagtgag gtgaaannnc atccagcaga tcaacctgtg gcgtgactcg gcaccacttt cctggtgcac cnccaggcga gangcgctgc agtgctacta ctactcctgg cnanacgctt nnngcnnnat gnnnagtann gnntananna nnnnnnnnnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMS LKHVASFGAV GVLSVVGVLG GTRWRRVELR RAELNEEYTK LMTEMRTFNE ERLTRDGRLA AKEVEAKATA ETVDILWSDR LARYAEVNKD LHAYLAALPE AIGALKGLSN HYRYMSEEMT KFMGFDIACS KMHNLALMLE HGKAVGIERV AAAVQEMFAA EPLVQTVCTS ILAAPAPPHP SSIAAASATF TFCMEELDRA VGKVAMRYAA ALEELPNATP GILSDSVRKL VGMTRADTLS KGQRQLAERR RDLERMLRRA QRQLHTEEDI RAALDYTREL DQHLQAAAPK RTDLLLSLPS RKDNFLAAVR SDSEVKKAIQ QINLWRDSAT TFLVHRQAED ALQSYYLLLA ETLTAVNELK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac tcaagcatgt ggcgtctttt ggcgctgttg gtgtcttatc ggtagtcggc gtacttggag gaacgcgatg gcgccgcgtc gagctgcgcc gcgcggagct aaacgaggag tacacgaagc tcatgactga aatgcgtacc ttcaacgagg agcgtctcac ccgcgatggg cgcctggccg ccaaggaggt ggaggcgaag gcgacggcgg agacggtgga catattatgg agcgatcggc tggcgcgtta tgcggaggtg aacaaggacc tgcatgccta cctggccgcc ttgcctgagg cgattggagc actgaagggt ctgtcgaacc actatcgcta catgtcggag gagatgacca agttcatggg gttcgacatc gcgtgctcca agatgcacaa cctggcactg atgctggagc acggcaaggc ggtaggcatt gagcgagtgg cggcggcggt gcaggagatg tttgccgcgg agccgcttgt gcagacggtg tgcacgagca tcttggcagc cccggctccg ccgcatccca gttcgatcgc tgcggccagc gccaccttca cgttctgcat ggaagagctc gaccgcgccg tggggaaggt tgctatgcgg tatgcggcag cactggaaga gctcccaaat gccacgcctg gcatcttgtc agacagtgtc cggaagcttg tcggcatgac gcgagcagac acgctcagca aggggcagcg ccagcttgcg gagcgccgaa gggacttgga acggatgctt cgccgtgccc agcgccagct gcacaccgag gaggacattc gggcagcgct ggattacacg agggagctgg atcagcatct tcaggcggcg gcaccgaaaa gaaccgactt gttgctttca ttgccttcca ggaaggataa ctttctggcc gccgtgcggt cggacagtga ggtgaaaaag gccatccagc agatcaacct gtggcgtgac tcggcaacca ctttcctggt gcaccgccag gccgaggacg cgctgcagag ctactactta ctcctggccg agacgcttac agcagtgaat gagttgaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat