LeinA.18709.a

serine/threonine-protein kinase, putative (AUK2)

CENTER ID: LeinA.18709.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18709.a.B2.GE42795 PHoranyi kinase domain truncation 260 587
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.29.1420
UniProt: E9AHI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSPCPSSSS PLLGSITSGI RSPSRGPVQQ QQQQHQLHAP MRLRLHEDGN IRACPEKSGG GVSQACAPPL PAASAFARGK EEPSAKRHHA EPSSCPAPDG DSSSVGRSAA ATAVTIAAPL LSISNPSSRA APSPFRFLCT SLGGCPVVGA KGARVHLPGS SASCDVSGSV APRDLSTTSA HSKAHSTAAV VRASPSSGVV SPPLSATYPS LVSASRGARV VCSHPQPASA ASVPGAIIDT SAPSPVPSRP HRIHQHELRP ADFRREEVLG EGSYSIVTLA THRASGLCFA LKEIDRSRLR WRPLEAQLRW EINLQRTLRH PHIVRLYSYF ITPDCISLVL EYCSGGTLLS RLRAAPQHRL SERQASRYTR HVAKALTHLH GLGVAHRDLK LENVLIDADG IAKLADFGWS RPVVHPLPPS KSAATTQTAG DRNDVPCSSA EHDTSNEDPT EGVAEGRRTV CGTLDYLSPE MVSGQAHSAK TDVWSLGVML AEMLTGTPPF YTESTQQTLY AIQRLPPNLT GLRPKGRGPG LTGSGASATA NVCLTLDEPV ALSAGALSLI HAMLQKDPSA RPTMQEVLSH AWLQKTR
NT Sequence
atgccatcgc catgcccctc ctcatcctcg cctctgctag gcagcatcac cagcggtatt cgctcgccgt caagaggtcc agtgcagcag cagcagcagc agcaccagct acatgccccg atgcgactgc gtctacacga ggacggcaac attcgcgcat gcccagagaa gtctggcgga ggtgtcagcc aagcgtgcgc tccgccgctg ccggctgctt cggcctttgc tcgagggaag gaggaaccga gcgcaaagcg tcatcatgca gagccgtcat cttgcccggc accagacggc gacagcagtt ctgtcggcag aagtgcggcg gcgacagccg tcaccattgc cgctcctctg ctttctatat caaacccctc ttcgcgggct gcgccttcgc cttttcgttt tctgtgcaca tctctcggtg ggtgcccggt ggtcggggcg aagggtgcac gcgttcacct gccaggctcg tcagcaagtt gcgacgtgag cggcagtgtc gctccgaggg acctttcgac cacatctgcg cactcaaagg cgcattccac ggcggccgtt gtgcgggcct cgccctcgtc gggcgtcgtt tcaccaccgc tctctgcgac ttacccctcc ctggtatctg cttctcgcgg cgcgcgcgtc gtttgctctc atcctcagcc cgcatcggcg gcttcagtgc ctggcgctat cattgacaca tcagcaccgt cgccagtgcc atcgcggccg caccgcatcc atcaacacga gcttcgcccc gcagatttcc gtcgtgagga ggtgctcggt gaggggagct attcgatcgt cacccttgcc acgcaccgtg cgtcgggcct gtgcttcgcg ctgaaggaga tcgaccgaag ccgcctgcga tggcgaccgc tggaggcgca gctgcgctgg gagatcaatt tgcagcggac gctgcgccac ccgcacatcg tgcgactcta cagctacttc attacgccag actgcatcag tctcgtgctg gagtactgca gcggcgggac gctgctgagt cggttgagag ctgcaccaca gcaccgcttg tcggagcggc aggcatcgcg ctacactcgc cacgtggcga aggcgctgac acatctgcac gggctcggag tggcgcaccg agacctgaag ctggaaaatg tgctcatcga tgcagacggc attgccaagc tggcagactt tggctggtca cgccctgttg tgcatccatt gccacccagt aagtcagcgg ctactactca gactgcaggc gacaggaacg acgtaccgtg tagcagcgcg gagcacgaca cgagcaacga ggacccgacg gaaggggtgg cagagggtcg ccgcaccgtg tgtggcactc tggactacct ctcgcctgag atggtctccg gccaagcaca ctcggccaag acagacgtct ggtcgcttgg tgtcatgctg gccgagatgc tgaccggcac gccgcccttc tacactgagt cgactcagca gacgctttac gccatccagc ggctgccgcc caacctcacc ggcttacgcc ctaaaggccg cgggccaggg ctcactggta gcggtgcctc cgccacggct aatgtgtgtc tgaccctcga cgaacccgtg gccctatctg caggtgcgct ctctctcatt cacgctatgc tccagaagga cccgtcggcg cgaccgacga tgcaggaggt gttgagccat gcctggctgc aaaaaactcg ttag
Details for LeinA.18709.a.B2.GE42795
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP ADFRREEVLG EGSYSIVTLA THRASGLCFA LKEIDRSRLR WRPLEAQLRW EINLQRTLRH PHIVRLYSYF ITPDCISLVL EYCSGGTLLS RLRAAPQHRL SERQASRYTR HVAKALTHLH GLGVAHRDLK LENVLIDADG IAKLADFGWS RPVVHPLPPS KSAATTQTAG DRNDVPCSSA EHDTSNEDPT EGVAEGRRTV CGTLDYLSPE MVSGQAHSAK TDVWSLGVML AEMLTGTPPF YTESTQQTLY AIQRLPPNLT GLRPKGRGPG LTGSGASATA NVCLTLDEPV ALSAGALSLI HAMLQKDPSA RPTMQEVLS
Validated NT Sequence
ggctcaacac ctcctgcatc gtcggtcgcg ccgacgggtc cttctggagc atagcgtgaa tgagagagag cgcacctgca gatagggcca cgggttcgtc gagggtcaga cacacattag ccgtggcgga ggcaccgcta ccagtgagcc ctggcccgcg gcctttaggg cgtaagccgg tgaggttggg cggcagccgc tggatggcgt aaagcgtctg ctgagtcgac tcagtgtaga agggcggcgt gccggtcagc atctcggcca gcatgacacc aagcgaccag acgtctgtct tggccgagtg tgcttggccg gagaccatct caggcgagag gtagtccaga gtgccacaca cggtgcggcg accctctgcc accccttccg tcgggtcctc gttgctcgtg tcgtgctccg cgctgctaca cggtacgtcg ttcctgtcgc ctgcagtctg agtagtagcc gctgacttac tgggtggcaa tggatgcaca acagggcgtg accagccaaa gtctgccagc ttggcaatgc cgtctgcatc gatgagcaca ttttccagct tcaggtctcg gtgcgccact ccgagcccgt gcagatgtgt cagcgccttc gccacgtggc gagtgtagcg cgatgcctgc cgctccgaca agcggtgctg tggtgcagct ctcaaccgac tcagcagcgt cccgccgctg cagtactcca gcacgagact gatgcagtct ggcgtaatga agtagctgta gagtcgcacg atgtgcgggt ggcgcagcgt ccgctgcaaa ttgatctccc agcgcagctg cgcctccagc ggtcgccatc gcaggcggct tcggtcgatc tccttcagcg cgaagcacag gcccgacgca cggtgcgtgg caagggtgac gatcgaatag ctcccctcac cgagcacctc ctcacgacgg aaatctgcgg gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHPA DFRREEVLGE GSYSIVTLAT HRASGLCFAL KEIDRSRLRW RPLEAQLRWE INLQRTLRHP HIVRLYSYFI TPDCISLVLE YCSGGTLLSR LRAAPQHRLS ERQASRYTRH VAKALTHLHG LGVAHRDLKL ENVLIDADGI AKLADFGWSR PVVHPLPPSK SAATTQTAGD RNDVPCSSAE HDTSNEDPTE GVAEGRRTVC GTLDYLSPEM VSGQAHSAKT DVWSLGVMLA EMLTGTPPFY TESTQQTLYA IQRLPPNLTG LRPKGRGPGL TGSGASATAN VCLTLDEPVA LSAGALSLIH AMLQKDPSAR PTMQEVLSHA WLQKTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcccgcag atttccgtcg tgaggaggtg ctcggtgagg ggagctattc gatcgtcacc cttgccacgc accgtgcgtc gggcctgtgc ttcgcgctga aggagatcga ccgaagccgc ctgcgatggc gaccgctgga ggcgcagctg cgctgggaga tcaatttgca gcggacgctg cgccacccgc acatcgtgcg actctacagc tacttcatta cgccagactg catcagtctc gtgctggagt actgcagcgg cgggacgctg ctgagtcggt tgagagctgc accacagcac cgcttgtcgg agcggcaggc atcgcgctac actcgccacg tggcgaaggc gctgacacat ctgcacgggc tcggagtggc gcaccgagac ctgaagctgg aaaatgtgct catcgatgca gacggcattg ccaagctggc agactttggc tggtcacgcc ctgttgtgca tccattgcca cccagtaagt cagcggctac tactcagact gcaggcgaca ggaacgacgt accgtgtagc agcgcggagc acgacacgag caacgaggac ccgacggaag gggtggcaga gggtcgccgc accgtgtgtg gcactctgga ctacctctcg cctgagatgg tctccggcca agcacactcg gccaagacag acgtctggtc gcttggtgtc atgctggccg agatgctgac cggcacgccg cccttctaca ctgagtcgac tcagcagacg ctttacgcca tccagcggct gccgcccaac ctcaccggct tacgccctaa aggccgcggg ccagggctca ctggtagcgg tgcctccgcc acggctaatg tgtgtctgac cctcgacgaa cccgtggccc tatctgcagg tgcgctctct ctcattcacg ctatgctcca gaaggacccg tcggcgcgac cgacgatgca ggaggtgttg agccatgcct ggctgcaaaa aactcgttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at