SSGCID: Target Page

LeinA.18708.a

isoleucyl-tRNA synthetase, putative

CENTER ID:	LeinA.18708.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18708.a.B3.GE42609	soluble domain #2		814	1100
LeinA.18708.a.B4.GE43058	C-term truncation		1	828

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LinJ.36.5870
UniProt:	A4IDX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGEGQQQQQ QDPPAGHQGV PVTNYPDVLD FSKMEEEVLA MWREKDCFRT SMKLSEGRQP FSFYDGPPFA TGLPHYGHIL AGTIKDMVTR FAYQTNHHVI RRFGWDCHGL PVEYEIDKML GIKTSHDVAK LGIDKYNDEC KKIVTRYVDE WRKTVERVGR WIDFDNDYKT MHLTYMESIW WVFSQLWEKK MVYRGFRVMP YSTACTTPLS NFEANSNYKE VSDLAVTVAF QARADPNTYF LAWTTTPWTL PSNLSLCVHP ELDYVKVLDN KSKRHYWLAE ARLAEVYPKK DSKKASKAKD VDNAAEADAA PYTVVEKVKG AQLVGEKYVP LFPYFESSMS ATAFRVISGT YVTTDAGTGI VHQAPAFGED DYQACLDAGI FEKGGKFVCP VDENGMFTSE VTDFAGKYVK AADPEIIKAL ETKGHLFHKA SIVHSYPFCW RSDTPLIYKA VESWFVNVEA FRDRLIECND KTYWVPDFVK TRRFSNWLAE ARDWNVSRNR YWGTPMPVWH SEDWEEVVCI SSIKQLEELS GVTGITDIHR QFVDDITIPS KRPGMPPLKR VPMVFDCWFE SGSMPYAQEH FPFENQEKFK SLFPADFVSE GLDQTRGWFY TLLVLGVALF DVSPFRNVAV SGLVLAEDGK KMSKRLKNYP EPKVVIDTYG ADALRMYMIS SPVVRAEPLR FREAGVKAVV KDILLPLFNA AKFFISNTNY CTAAGGQVSL QVRSTNEMDR WILASCQSLL RYVKAEMRLY HLYNVVPGIL RFVGDLSNWY VRMNRRRMKN ATDSEDRSQA LSTMLYLLFS VSRIVGHIAP FVAEMLYQQI KPLLPANEQV DSVHYLMIPD EDTSLDDPEL ERAMSRMMNI VDLVRVLRDQ MVIPIKRPVR QVVIVHPDQQ YIDDVRKVAG YIKDEVNAFE IVMSSGENYL ETKLDANFEV LGRKYRKEMP AIRKGIQAMT QNEVAKFLHD KKGVVVGKEL TIEDVKVLRQ VKEGIADFQS NTDNDVVVLV DKRQDQELID SWRAREFVSR VQQLRKKAKL VVTDEVDVYF EAEEADLTAS ILHSKDQINQ TLRGVWTTMD QKPSDAELVA EEDNNISGVA VRLVFTKPKA

NT Sequence

atggcgggcg aaggtcagca gcagcagcag caagaccccc cagcggggca tcagggcgta ccggtgacga actatcccga tgtgctcgac ttctcgaaga tggaggagga ggtgctggcg atgtggcggg agaaggattg cttccgcact tccatgaagc tctccgaggg ccgccagccg ttcagcttct acgacggccc accgttcgcg actggcctgc cgcactacgg ccacatcttg gctggcacca tcaaggacat ggtcactcgc ttcgcctacc agacgaacca ccacgtcatc cgtcgcttcg gctgggactg ccacggtctg ccggtggagt acgagatcga caagatgctc ggcatcaaga cgtctcacga cgtggcgaag ctcggcattg acaagtataa cgacgagtgc aagaagatcg tcacccgcta cgtggacgaa tggaggaaga ccgtcgagcg cgttggccgc tggatcgact ttgacaacga ctacaagacg atgcacctca catacatgga gagtatctgg tgggttttct cgcagctgtg ggagaagaag atggtgtacc gcggctttcg cgtgatgccg tactcgaccg cctgcacgac gccgctgagc aacttcgagg ctaactccaa ctacaaggag gtcagcgacc tcgccgtgac ggtcgccttc caggcccgcg ccgaccccaa cacatacttc ctggcctgga ccaccacgcc gtggacgttg ccgagcaacc tcagtttgtg cgtgcacccc gagctggact acgtgaaggt gctggacaac aagtcgaagc gacactactg gctcgcagag gcccgcctgg cggaggtgta cccgaagaag gacagcaaga aggcctccaa ggcgaaggac gtggacaacg ccgcggaggc cgatgccgcg ccgtacacgg tcgtcgagaa agtcaagggc gcgcagctag tgggcgagaa gtacgtgcca cttttcccgt actttgagtc gtccatgagc gccacggcct tccgcgtcat ctccggtacc tacgtgacga cagacgctgg tacaggtatc gtgcaccagg cccccgcttt cggtgaggat gattaccagg cgtgcctcga cgccggcatc ttcgagaagg gcggcaagtt cgtctgcccg gtcgacgaga acggcatgtt cacgagcgag gtgacggatt tcgccggcaa gtacgtcaag gcggcggatc cggagattat caaagcgctc gagacgaaag ggcacctctt tcacaaggcg tcgattgtgc acagctaccc cttctgctgg cgcagcgaca cccccctcat ctacaaggct gtcgagtcgt ggtttgtcaa cgtcgaggcc ttccgcgacc gcctcatcga gtgcaacgac aagacgtact gggtacctga ttttgtcaag acccgccgct tctccaactg gctcgccgag gcgcgtgact ggaacgtgtc acgcaaccgt tactggggca cgccgatgcc ggtctggcac agcgaggact gggaggaggt tgtgtgcatc agcagcatca agcagctcga ggagctctcc ggcgtcaccg gcatcaccga catccaccgc cagttcgtcg acgatatcac gatcccaagt aagcggcctg gcatgccgcc gctgaagcgt gtgccgatgg tgtttgactg ctggttcgag tccggctcga tgccgtacgc ccaggagcac ttccctttcg agaaccagga gaagttcaag agcctgtttc cggccgactt tgtgtcggag ggcttggacc agacgcgcgg ctggttctac acccttctcg tgctcggcgt tgccctcttc gacgtctccc ctttccgcaa cgtcgccgtc agcgggctcg tgctcgccga ggacggtaag aagatgagca agcgcctcaa gaactaccct gagccgaagg tggtgatcga cacgtacggc gcggatgccc tgcgcatgta catgattagc tccccggttg tgcgcgccga gccgctgcgc ttccgcgaag caggtgtgaa ggccgtggtc aaggacatcc ttctgcctct gttcaacgcc gcgaagttct tcatctcgaa cacaaactac tgcactgcgg ccggtggtca ggtgtcgctg caggttcgca gcacaaacga gatggaccgc tggattctcg cctcgtgcca gagtctgctg cgctacgtga aggcggagat gcggctgtac cacctgtaca acgtcgtccc cggcattctg cgtttcgtcg gtgacctgtc caactggtac gtgcgcatga accgccgccg catgaagaac gccacggaca gcgaggaccg ctcgcaggcg ctgtcgacga tgctgtacct cctcttctcg gtgtcccgca ttgtgggcca catcgccccc tttgtggcag aaatgcttta ccagcagatc aagcccctcc tcccggcgaa cgagcaagtg gactccgtgc actacctcat gatccccgac gaggatacct ccttggacga cccggagctg gagcgcgcca tgtcccgcat gatgaacatt gtggatttgg tgcgtgtgtt gcgcgatcag atggtcattc cgatcaagcg gccggtgcgt caggttgtca ttgttcaccc ggaccagcag tacatcgacg acgtgcgcaa ggtggcgggc tacatcaagg atgaggtgaa cgcgttcgag attgtgatgt cgagtggcga gaattacctg gagacgaagc tggacgcaaa cttcgaggtg cttggcagga agtaccgcaa ggagatgccc gcgatccgca agggtatcca ggccatgacg cagaatgagg tggccaagtt cctgcacgac aagaagggcg tcgtcgtcgg caaggagctg accatcgagg acgtgaaggt gctgcgccag gtaaaggagg gcattgccga cttccagagc aacactgaca acgacgtcgt tgtgctggtt gacaagcgcc aggatcagga actgattgac tcgtggcgcg cgcgtgagtt cgtgagccgc gtacagcagc tgcgtaagaa ggcgaagcta gtggtgacgg acgaggtgga tgtgtacttt gaggcggagg aggcggatct gacggcgtcc atcctccact ctaaggacca gattaatcag acactgaggg gcgtgtggac gacgatggac cagaagccta gcgacgccga gctcgtcgcg gaggaggaca ataacatctc gggggtggcg gtgcgcctcg tcttcaccaa gcccaaggct taa

Details for LeinA.18708.a.B3.GE42609

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME MLYQQIKPLL PANEQVDSVH YLMIPDEDTS LDDPELERAM SRMMNIVDLV RVLRDQMVIP IKRPVRQVVI VHPDQQYIDD VRKVAGYIKD EVNAFEIVMS SGENYLETKL DANFEVLGRK YRKEMPAIRK GIQAMTQNEV AKFLHDKKGV VVGKELTIED VKVLRQVKEG IADFQSNTDN DVVVLVDKRQ DQELIDSWRA REFVSRVQQL RKKAKLVVTD EVDVYFEAEE ADLTASILHS KDQINQTLRG VWTTMDQKPS DAELVAEEDN NISGVAXXXX XTKPKA

Validated NT Sequence

ttactcactt aagccttggg cttggtnnnn nnnnnnnnnn ccgccacccc cgagatgtta ttgtcctcct ccgcgacgag ctcggcgtcg ctaggcttct ggtccatcgt cgtccacacg cccctcagtg tctgattaat ctggtcctta gagtggagga tggacgccgt cagatccgcc tcctccgcct caaagtacac atccacctcg tccgtcacca ctagcttcgc cttcttacgc agctgctgta cgcggctcac gaactcacgc gcgcgccacg agtcaatcag ttcctgatcc tggcgcttgt caaccagcac aacgacgtcg ttgtcagtgt tgctctggaa gtcggcaatg ccctccttta cctggcgcag caccttcacg tcctcgatgg tcagctcctt gccgacgacg acgcccttct tgtcgtgcag gaacttggcc acctcattct gcgtcatggc ctggataccc ttgcggatcg cgggcatctc cttgcggtac ttcctgccaa gcacctcgaa gtttgcgtcc agcttcgtct ccaggtaatt ctcgccactc gacatcacaa tctcgaacgc gttcacctca tccttgatgt agcccgccac cttgcgcacg tcgtcgatgt actgctggtc cgggtgaaca atgacaacct gacgcaccgg ccgcttgatc ggaatgacca tctgatcgcg caacacacgc accaaatcca caatgttcat catgcgggac atggcgcgct ccagctccgg gtcgtccaag gaggtatcct cgtcggggat catgaggtag tgcacggagt ccacttgctc gttcgccggg aggaggggct tgatctgctg gtaaagcatt tccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHEM LYQQIKPLLP ANEQVDSVHY LMIPDEDTSL DDPELERAMS RMMNIVDLVR VLRDQMVIPI KRPVRQVVIV HPDQQYIDDV RKVAGYIKDE VNAFEIVMSS GENYLETKLD ANFEVLGRKY RKEMPAIRKG IQAMTQNEVA KFLHDKKGVV VGKELTIEDV KVLRQVKEGI ADFQSNTDND VVVLVDKRQD QELIDSWRAR EFVSRVQQLR KKAKLVVTDE VDVYFEAEEA DLTASILHSK DQINQTLRGV WTTMDQKPSD AELVAEEDNN ISGVAVRLVF TKPKA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaatgc tttaccagca gatcaagccc ctcctcccgg cgaacgagca agtggactcc gtgcactacc tcatgatccc cgacgaggat acctccttgg acgacccgga gctggagcgc gccatgtccc gcatgatgaa cattgtggat ttggtgcgtg tgttgcgcga tcagatggtc attccgatca agcggccggt gcgtcaggtt gtcattgttc acccggacca gcagtacatc gacgacgtgc gcaaggtggc gggctacatc aaggatgagg tgaacgcgtt cgagattgtg atgtcgagtg gcgagaatta cctggagacg aagctggacg caaacttcga ggtgcttggc aggaagtacc gcaaggagat gcccgcgatc cgcaagggta tccaggccat gacgcagaat gaggtggcca agttcctgca cgacaagaag ggcgtcgtcg tcggcaagga gctgaccatc gaggacgtga aggtgctgcg ccaggtaaag gagggcattg ccgacttcca gagcaacact gacaacgacg tcgttgtgct ggttgacaag cgccaggatc aggaactgat tgactcgtgg cgcgcgcgtg agttcgtgag ccgcgtacag cagctgcgta agaaggcgaa gctagtggtg acggacgagg tggatgtgta ctttgaggcg gaggaggcgg atctgacggc gtccatcctc cactctaagg accagattaa tcagacactg aggggcgtgt ggacgacgat ggaccagaag cctagcgacg ccgagctcgt cgcggaggag gacaataaca tctcgggggt ggcggtgcgc ctcgtcttca ccaagcccaa ggcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LeinA.18708.a.B4.GE43058

HARVESTED ON:	4/19/2019
SEQUENCED ON:	4/23/2019
EXPECTED MW:	96kDa
OBSERVED MW:	96kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA GEGQQQQQQD PPAGHQGVPV TNYPDVLDFS KMEEEVLAMW REKDCFRTSM KLSEGRQPFS FYDGPPFATG LPHYGHILAG TIKDMVTRFA YQTNHHVIRR FGWDCHGLPV EYEIDKMLGI KTSHDVAKLG IDKYNDECKK IVTRYVDEWR KTVERVGRWI DFDNDYKTMH LTYMESIWWV FSQLWEKKMV YRGFRVMPYS TACTTPLSNF EANSNYKEVS DLAVTVAFQA RADPNTYFLA WTTTPWTLPS NLSLCVHPEL DYVKVLDNKS KRHYWLAEAR LAEVYPKKDS KKASKAKDVD NAAEADAAPY TVVEKVKGAQ LVGEKYVPLF PYFESSMSAT AFRVISGTYV TTDAGTGIVH QAPAFGEDDY QACLDAGIFE KGGKFVCPVD ENGMFTSEVT DFAGKYVKAA DPEIIKALET KGHLFHKASI VHSYPFCWRS DTPLIYKAVE SWFVNVEAFR DRLIECNDKT YWVPDFVKTR RFSNWLAEAR DWNVSRNRYW GTPMPVWHSE DWEEVVCISS IKQLEELSGV TGITDIHRQF VDDITIPSKR PGMPPLKRVP MVFDCWFESG SMPYAQEHFP FENQEKFKSL FPADFVSEGL DQTRGWFYTL LVLGVALFDV SPFRNVAVSG LVLAEDGKKM SKRLKNYPEP KVVIDTYGAD ALRMYMISSP VVRAEPLRFR EAGVKAVVKD ILLPLFNAAK FFISNTNYCT AAGGQVSLQV RSTNEMDRWI LASCQSLLRY VKAEMRLYHL YNVVPGILRF VGDLSNWYVR MNRRRMKNAT DSEDRSQALS TMLYLLFSVS RIVGHIAPFV AEMLYQQIKP LLPANE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gcgaaggtca gcagcagcag cagcaagacc ccccagcggg gcatcagggc gtaccggtga cgaactatcc cgatgtgctc gacttctcga agatggagga ggaggtgctg gcgatgtggc gggagaagga ttgcttccgc acttccatga agctctccga gggccgccag ccgttcagct tctacgacgg cccaccgttc gcgactggcc tgccgcacta cggccacatc ttggctggca ccatcaagga catggtcact cgcttcgcct accagacgaa ccaccacgtc atccgtcgct tcggctggga ctgccacggt ctgccggtgg agtacgagat cgacaagatg ctcggcatca agacgtctca cgacgtggcg aagctcggca ttgacaagta taacgacgag tgcaagaaga tcgtcacccg ctacgtggac gaatggagga agaccgtcga gcgcgttggc cgctggatcg actttgacaa cgactacaag acgatgcacc tcacatacat ggagagtatc tggtgggttt tctcgcagct gtgggagaag aagatggtgt accgcggctt tcgcgtgatg ccgtactcga ccgcctgcac gacgccgctg agcaacttcg aggctaactc caactacaag gaggtcagcg acctcgccgt gacggtcgcc ttccaggccc gcgccgaccc caacacatac ttcctggcct ggaccaccac gccgtggacg ttgccgagca acctcagttt gtgcgtgcac cccgagctgg actacgtgaa ggtgctggac aacaagtcga agcgacacta ctggctcgca gaggcccgcc tggcggaggt gtacccgaag aaggacagca agaaggcctc caaggcgaag gacgtggaca acgccgcgga ggccgatgcc gcgccgtaca cggtcgtcga gaaagtcaag ggcgcgcagc tagtgggcga gaagtacgtg ccacttttcc cgtactttga gtcgtccatg agcgccacgg ccttccgcgt catctccggt acctacgtga cgacagacgc tggtacaggt atcgtgcacc aggcccccgc tttcggtgag gatgattacc aggcgtgcct cgacgccggc atcttcgaga agggcggcaa gttcgtctgc ccggtcgacg agaacggcat gttcacgagc gaggtgacgg atttcgccgg caagtacgtc aaggcggcgg atccggagat tatcaaagcg ctcgagacga aagggcacct ctttcacaag gcgtcgattg tgcacagcta ccccttctgc tggcgcagcg acacccccct catctacaag gctgtcgagt cgtggtttgt caacgtcgag gccttccgcg accgcctcat cgagtgcaac gacaagacgt actgggtacc tgattttgtc aagacccgcc gcttctccaa ctggctcgcc gaggcgcgtg actggaacgt gtcacgcaac cgttactggg gcacgccgat gccggtctgg cacagcgagg actgggagga ggttgtgtgc atcagcagca tcaagcagct cgaggagctc tccggcgtca ccggcatcac cgacatccac cgccagttcg tcgacgatat cacgatccca agtaagcggc ctggcatgcc gccgctgaag cgtgtgccga tggtgtttga ctgctggttc gagtccggct cgatgccgta cgcccaggag cacttccctt tcgagaacca ggagaagttc aagagcctgt ttccggccga ctttgtgtcg gagggcttgg accagacgcg cggctggttc tacacccttc tcgtgctcgg cgttgccctc ttcgacgtct cccctttccg caacgtcgcc gtcagcgggc tcgtgctcgc cgaggacggt aagaagatga gcaagcgcct caagaactac cctgagccga aggtggtgat cgacacgtac ggcgcggatg ccctgcgcat gtacatgatt agctccccgg ttgtgcgcgc cgagccgctg cgcttccgcg aagcaggtgt gaaggccgtg gtcaaggaca tccttctgcc tctgttcaac gccgcgaagt tcttcatctc gaacacaaac tactgcactg cggccggtgg tcaggtgtcg ctgcaggttc gcagcacaaa cgagatggac cgctggattc tcgcctcgtg ccagagtctg ctgcgctacg tgaaggcgga gatgcggctg taccacctgt acaacgtcgt ccccggcatt ctgcgtttcg tcggtgacct gtccaactgg tacgtgcgca tgaaccgccg ccgcatgaag aacgccacgg acagcgagga ccgctcgcag gcgctgtcga cgatgctgta cctcctcttc tcggtgtccc gcattgtggg ccacatcgcc ccctttgtgg cagaaatgct ttaccagcag atcaagcccc tcctcccggc gaacgagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at