LeinA.18681.b

prolyl-tRNA synthetase, putative

CENTER ID: LeinA.18681.b
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18681.b.B1.GE42607 full length 1 722
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18681.b.D11.PD38417 full length 216 722

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.18.1230
UniProt: A4HY00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKDCRGEP ELLALLKELN IELPTISHGE MHTVEEANKE LGRFGTPCVG TKNMFLKSRK GELVLLTAVH TTKTDMHAIE KAAGTKNLRF APAEILSDNL AVVQGCVTPF ALINNIEKRN VTVLLDKNLK ESPIPFVLHP CRNDKSSLVV FEQLERFLEK LGYAYKLVDF GAASADAAVT GGAAAPAPKP KKAPADAAAA AAAAPASAQS GETKLGIAAK REENFSAWYI DVITKAEMIE YYDVSGCYII RPWAYYVWKC VQRFLGGKIE KLGVEDCYFP MFVSRNCLER EKDHIEGFAP EVAWVTRAGD TELEQPVAVR PTSETVMYPY YAKWIRSHRD LPVRLNMWNN VIRWEFSHPT PFIRTREFLW QEGHCAWAKA EECAKEVLDI LECYASVYEQ LLAVPVVRGR KTEKFAGGDY TTTVETFIEA VGRGCQGATS HNLGQNFGKM FDIRFQDPEN NEQTLIPWQN SWGLSTRVIG VMIMVHGDNR GMVMPPRVAS TQVIIIPVGI TKDTTEEARQ ELLASCRRLE SELCEGGVRA KCDLRDNYSP GWRFNHWEVK GVPLRVELGP RELAERSLAV AVRHSGARHS VVWDAQTPTA VAALLEDVHA QMYARAKETM ETHRVRVTEW AEFVPTLNRK CLILAPWCGA MECEDQVKKD SAEESKAAQA QETREDARAP SMGAKTLCIP FEQPEDPAEG HGCICKGCTK PATTWVLFGR SY
NT Sequence
atgtccgcga aggactgcag gggagagccg gagctgctgg cgctgttgaa ggagctcaac attgagctgc ccactatctc gcatggggag atgcacacgg tagaagaggc gaataaggag ctgggccgct ttggcactcc ttgtgtgggc accaagaaca tgttcctcaa gagcaggaag ggcgaactgg tactgctcac agcggtacac acgaccaaga cggacatgca tgcaatcgag aaggcggccg gaaccaagaa tctccggttt gccccggcag agatcctcag cgacaatctc gccgtcgtgc agggctgcgt cacaccgttt gccctcatca acaatatcga gaagcgcaac gtcacggtgc tcttggataa gaacctgaag gagagcccca tccccttcgt gctacacccc tgccgcaacg ataagagctc tctcgttgtc tttgagcaac tggagcgttt cctggagaag ctcgggtatg cgtacaagct ggtggacttt ggtgcggctt cggcggacgc tgcggtgacg ggcggcgctg ctgctcctgc tccgaagccg aagaaggcgc ccgcggacgc tgctgctgct gctgctgctg cgcctgcgtc tgcacagtct ggcgagacga agctgggcat cgcggcgaag cgcgaggaga acttctctgc gtggtacatt gatgtgatca cgaaggcgga gatgatcgag tactacgacg tgtccgggtg ctacattatt cgtccgtggg cgtactacgt gtggaagtgc gtgcagcgct tccttggtgg gaagatcgag aagcttggcg tggaggactg ctacttcccg atgttcgtgt cgcgcaactg cctggagcgg gagaaggacc acatcgaggg ctttgcgccg gaggttgcat gggtgacgcg cgccggcgac acggagctgg agcagccggt tgccgtgcgg ccgacgagcg agacggtgat gtacccgtac tacgcgaagt ggatccggtc gcaccgcgac ctgcccgtgc ggctgaacat gtggaacaac gtgatccggt gggagttctc gcacccgacg ccgttcattc ggactcgcga gttcctgtgg caggaggggc actgtgcgtg ggcgaaggcg gaggagtgcg cgaaggaggt gctggacatc ctggagtgct atgcatcggt gtacgagcag ctgctggcgg tgccggtggt gcgcgggcgc aagacggaga agttcgctgg cggggactac acgacgacgg tggagacgtt catcgaggct gttgggcgcg ggtgccaggg cgcgacgagc cacaacctgg ggcagaactt cggcaagatg ttcgacatcc gcttccagga cccggagaac aacgagcaga cgctgatccc gtggcagaac agctgggggc tgtcgacgcg cgtgatcggc gtgatgatca tggtgcacgg cgacaaccgc ggcatggtga tgccgccgcg cgttgcgtcg acgcaggtga tcatcatccc tgtggggatc acgaaggaca cgacggagga ggcgcggcag gagctgcttg caagttgccg gcggctggag agcgagctgt gcgagggtgg cgtgcgcgcg aagtgcgacc tgcgcgacaa ctacagccct gggtggcggt tcaaccactg ggaggtgaag ggcgtgccac tgcgcgtgga gcttgggccg agggagcttg cggagcggtc gcttgcggtt gctgtgcggc acagcggcgc gaggcactcg gttgtgtggg acgcgcagac gccgacggcg gtagccgcac tgctggagga tgtgcatgca cagatgtacg cgcgcgcgaa ggagacgatg gagacgcatc gcgtgcgtgt gacggagtgg gcggagtttg tgccgacgct gaaccggaag tgcttgatcc ttgcgccgtg gtgcggcgcg atggagtgcg aggaccaggt aaagaaggac agcgcggagg agtcgaaggc tgcgcaggcg caggagacgc gcgaggacgc gcgtgcgccg agcatgggtg cgaagacgct gtgcatcccg tttgagcagc cggaggaccc cgcagagggt cacgggtgca tctgcaaggg ctgcacgaag ccggcgacga cgtgggtgct gtttggccgc agctactga
Details for LeinA.18681.b.B1.GE42607
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 82kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS AKDCRGEPEL LALLKELNIE LPTISHGEMH TVEEANKELG RFGTPCVGTK NMFLKSRKGE LVLLTAVHTT KTDMHAIEKA AGTKNLRFAP AEILSDNLAV VQGCVTPFAL INNIEKRNVT VLLDKNLKES PIPFVLHPCR NDKSSLVVFE QLERFLEKLG YAYKLVDFGA ASADAAVTGG AAAPAPKPKK APADAAAAAA AAPASAQSGE TKLGIAAKRE ENFSAWYIDV ITKAEMIEYY DVSGCYIIRP WAYYVWKCVQ RFLGGKIEKL GVEDCYFPMF VSRNCLEREK DHIEGFAPEV AWVTRAGDTE LEQPVAVRPT SETVMYPYYA KWIRSHRDLP VRLNMWNNVI RWEFSHPTPF IRTREFLWQE GHCAWAKAEE CAKEVLDILE CYASVYEQLL AVPVVRGRKT EKFAGGDYTT TVETFIEAVG RGCQGATSHN LGQNFGKMFD IRFQDPENNE QTLIPWQNSW GLSTRVIGVM IMVHGDNRGM VMPPRVASTQ VIIIPVGITK DTTEEARQEL LASCRRLESE LCEGGVRAKC DLRDNYSPGW RFNHWEVKGV PLRVELGPRE LAERSLAVAV RHSGARHSVV WDAQTPTAVA ALLEDVHAQM YARAKETMET HRVRVTEWAE FVPTLNRKCL ILAPWCGAME CEDQVKKDSA EESKAAQAQE TREDARAPSM GAKTLCIPFE QPEDPAEGHG CICKGCTKPA TTWVLFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cgaaggactg caggggagag ccggagctgc tggcgctgtt gaaggagctc aacattgagc tgcccactat ctcgcatggg gagatgcaca cggtagaaga ggcgaataag gagctgggcc gctttggcac tccttgtgtg ggcaccaaga acatgttcct caagagcagg aagggcgaac tggtactgct cacagcggta cacacgacca agacggacat gcatgcaatc gagaaggcgg ccggaaccaa gaatctccgg tttgccccgg cagagatcct cagcgacaat ctcgccgtcg tgcagggctg cgtcacaccg tttgccctca tcaacaatat cgagaagcgc aacgtcacgg tgctcttgga taagaacctg aaggagagcc ccatcccctt cgtgctacac ccctgccgca acgataagag ctctctcgtt gtctttgagc aactggagcg tttcctggag aagctcgggt atgcgtacaa gctggtggac tttggtgcgg cttcggcgga cgctgcggtg acgggcggcg ctgctgctcc tgctccgaag ccgaagaagg cgcccgcgga cgctgctgct gctgctgctg ctgcgcctgc gtctgcacag tctggcgaga cgaagctggg catcgcggcg aagcgcgagg agaacttctc tgcgtggtac attgatgtga tcacgaaggc ggagatgatc gagtactacg acgtgtccgg gtgctacatt attcgtccgt gggcgtacta cgtgtggaag tgcgtgcagc gcttccttgg tgggaagatc gagaagcttg gcgtggagga ctgctacttc ccgatgttcg tgtcgcgcaa ctgcctggag cgggagaagg accacatcga gggctttgcg ccggaggttg catgggtgac gcgcgccggc gacacggagc tggagcagcc ggttgccgtg cggccgacga gcgagacggt gatgtacccg tactacgcga agtggatccg gtcgcaccgc gacctgcccg tgcggctgaa catgtggaac aacgtgatcc ggtgggagtt ctcgcacccg acgccgttca ttcggactcg cgagttcctg tggcaggagg ggcactgtgc gtgggcgaag gcggaggagt gcgcgaagga ggtgctggac atcctggagt gctatgcatc ggtgtacgag cagctgctgg cggtgccggt ggtgcgcggg cgcaagacgg agaagttcgc tggcggggac tacacgacga cggtggagac gttcatcgag gctgttgggc gcgggtgcca gggcgcgacg agccacaacc tggggcagaa cttcggcaag atgttcgaca tccgcttcca ggacccggag aacaacgagc agacgctgat cccgtggcag aacagctggg ggctgtcgac gcgcgtgatc ggcgtgatga tcatggtgca cggcgacaac cgcggcatgg tgatgccgcc gcgcgttgcg tcgacgcagg tgatcatcat ccctgtgggg atcacgaagg acacgacgga ggaggcgcgg caggagctgc ttgcaagttg ccggcggctg gagagcgagc tgtgcgaggg tggcgtgcgc gcgaagtgcg acctgcgcga caactacagc cctgggtggc ggttcaacca ctgggaggtg aagggcgtgc cactgcgcgt ggagcttggg ccgagggagc ttgcggagcg gtcgcttgcg gttgctgtgc ggcacagcgg cgcgaggcac tcggttgtgt gggacgcgca gacgccgacg gcggtagccg cactgctgga ggatgtgcat gcacagatgt acgcgcgcgc gaaggagacg atggagacgc atcgcgtgcg tgtgacggag tgggcggagt ttgtgccgac gctgaaccgg aagtgcttga tccttgcgcc gtggtgcggc gcgatggagt gcgaggacca ggtaaagaag gacagcgcgg aggagtcgaa ggctgcgcag gcgcaggaga cgcgcgagga cgcgcgtgcg ccgagcatgg gtgcgaagac gctgtgcatc ccgtttgagc agccggagga ccccgcagag ggtcacgggt gcatctgcaa gggctgcacg aagccggcga cgacgtgggt gctgtttggc cgcagctact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LeinA.18681.b.D11.PD38417
PURIFICATION DATe: 1/12/2020
CONCENTRATION: 5mg/ml
OBSERVED MW: 69kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM tris pH 8.0, 200mM NaCl, 1mM TCEP, 2mM proline
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 8
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
CID102218, PUR2152, PID8063-1
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSGAM GIAAKREENF SAWYIDVITK AEMIEYYDVS GCYIIRPWAY YVWKCVQRFL GGKIEKLGVE DCYFPMFVSR NCLEREKDHI EGFAPEVAWV TRAGDTELEQ PVAVRPTSET VMYPYYAKWI RSHRDLPVRL NMWNNVIRWE FSHPTPFIRT REFLWQEGHC AWAKAEECAK EVLDILECYA SVYEQLLAVP VVRGRKTEKF AGGDYTTTVE TFIEAVGRGC QGATSHNLGQ NFGKMFDIRF QDPENNEQTL IPWQNSWGLS TRVIGVMIMV HGDNRGMVMP PRVASTQVII IPVGITKDTT EEARQELLAS CRRLESELCE GGVRAKCDLR DNYSPGWRFN HWEVKGVPLR VELGPRELAE RSLAVAVRHS GARHSVVWDA QTPTAVAALL EDVHAQMYAR AKETMETHRV RVTEWAEFVP TLNRKCLILA PWCGAMECED QVKKDSAEES KAAQAQETRE DARAPSMGAK TLCIPFEQPE DPAEGHGCIC KGCTKPATTW VLFGRSY
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CGGTGCGATG GGCATTGCGG CGAAGCGTGA GGAAAACTTC AGCGCGTGGT ACATTGACGT GATCACCAAA GCGGAGATGA TCGAATACTA TGATGTTAGC GGTTGCTATA TCATTCGTCC GTGGGCGTAC TATGTGTGGA AGTGCGTTCA GCGTTTCCTG GGTGGCAAGA TTGAGAAACT GGGCGTGGAA GACTGCTACT TCCCGATGTT TGTTAGCCGT AACTGCCTGG AGCGTGAAAA AGATCACATC GAGGGTTTTG CGCCGGAAGT GGCGTGGGTT ACCCGTGCGG GTGACACCGA GCTGGAACAA CCGGTGGCGG TTCGTCCGAC CAGCGAGACC GTGATGTATC CGTACTATGC GAAGTGGATT CGTAGCCACC GTGATCTGCC GGTGCGTCTG AACATGTGGA ACAACGTTAT TCGTTGGGAA TTTAGCCACC CGACCCCGTT CATCCGTACC CGTGAGTTTC TGTGGCAGGA AGGTCATTGC GCGTGGGCGA AGGCGGAGGA ATGCGCGAAA GAGGTTCTGG ATATTCTGGA ATGCTACGCG AGCGTGTATG AGCAACTGCT GGCGGTTCCG GTGGTTCGTG GTCGTAAGAC CGAAAAATTC GCGGGTGGCG ACTACACCAC CACCGTGGAG ACCTTTATTG AAGCGGTTGG TCGTGGCTGC CAAGGTGCGA CCAGCCACAA CCTGGGTCAA AACTTCGGCA AGATGTTTGA CATTCGTTTC CAGGACCCGG AGAACAACGA ACAGACCCTG ATCCCGTGGC AAAACAGCTG GGGTCTGAGC ACCCGTGTGA TTGGCGTTAT GATCATGGTG CACGGTGATA ACCGTGGCAT GGTGATGCCG CCGCGTGTTG CGAGCACCCA GGTGATCATT ATCCCGGTTG GTATTACCAA AGACACCACC GAGGAAGCGC GTCAAGAGCT GCTGGCGAGC TGCCGTCGTC TGGAGAGCGA ACTGTGCGAA GGTGGCGTGC GTGCGAAGTG CGACCTGCGT GATAACTACA GCCCGGGTTG GCGTTTCAAC CACTGGGAAG TGAAAGGTGT TCCGCTGCGT GTTGAACTGG GTCCGCGTGA GCTGGCGGAA CGTAGCCTGG CGGTTGCGGT TCGTCACAGC GGTGCGCGTC ACAGCGTGGT TTGGGATGCG CAGACCCCGA CCGCGGTGGC GGCGCTGCTG GAGGATGTTC ACGCGCAAAT GTATGCGCGT GCGAAGGAGA CGATGGAGAC CCACCGTGTG CGTGTTACCG AGTGGGCGGA ATTTGTGCCG ACCCTGAACC GTAAATGCCT GATTCTGGCG CCGTGGTGCG GTGCGATGGA GTGCGAAGAC CAGGTTAAGA AAGATAGCGC GGAGGAAAGC AAAGCGGCGC AGGCGCAAGA GACCCGTGAA GATGCGCGTG CGCCGAGCAT GGGCGCGAAA ACCCTGTGCA TTCCGTTCGA ACAGCCGGAA GACCCGGCGG AGGGTCACGG CTGCATCTGC AAGGGTTGCA CCAAACCGGC GACCACCTGG GTTCTGTTTG GCCGTAGCTA CTGATAAGCT TGCGGCCGCA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GAT