SSGCID: Target Page

LeinA.18681.a

prolyl-tRNA synthetase, putative

CENTER ID:	LeinA.18681.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18681.a.B2.GE42606	soluble domain		24	810

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18681.a.B2.PW38683	soluble domain		24	810

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LinJ.18.1220
UniProt:	A4HXZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRGGRLLGPP HACFSLLSNS VSCLSSSRAS HRSITRHLGV RHHLPSSSPR TPSYSTSPVT PVTPTRGYSG TKIHTQNKSP PASSLPHMSI EDCRGFHEVK ALLQELGLDI PIVHHDEKAT VEEVLDLLHK MGIHAAGTKT LFLKSKKGEL VMATAFKSTP TDLKFIQKVT NAKDLRFASS EVLQECLAVV QGCVTPFALI NNIEKRPITL LIDRSLEAST LPLAFCLCRN DYTAVITFEE LKKYLEKLGH AYKLVDFGAA SADAAVTGGA AAPAPKPKKA PADAAAAAAA PASAQSGETK LGIAAKREEN FSAWYIDVIT KAEMIEYYDV SGCYIIRPWA YYVWKCVQRF LGGKIEKLGV EDCYFPMFVS RNCLEREKDH IEGFAPEVAW VTRAGDTELE QPVAVRPTSE TVMYPYYAKW IRSHRDLPVR LNMWNNVIRW EFSHPTPFIR TREFLWQEGH CAWAKAEECA KEVLDILECY ASVYEQLLAV PVVRGRKTEK EKFAGGDYTT TVETFIEAVG RGCQGATSHN LGQNFGKMFD IRFQDPENNE QTLIPWQNSW GLSTRVIGVM IMVHGDNRGM VMPPRVASTQ VIIIPVGITK DTTEEARQEL LASCRRLESE LCEGGVRAKC DLRDNYSPGW RFNHWEVKGV PLRVELGPRE LAERSLAVAV RHSGARHSVV WDAQTPTAVA ALLEDVHAQM YARAKETMET HRVRVTEWAE FVPTLNRKCL ILAPWCGAME CEDQVKKDSA EESKAAQAQE TREDARAPSM GAKTLCIPFE QPEDPAEGHG CICKGCTKPA TTWVLFGRSY

NT Sequence

atgcgtggtg gacggctctt gggcccacct cacgcgtgct tttctctgtt aagtaactct gtgtcttgcc tatcctccag tcgggcttcg catcgatcga tcacgcgcca cttgggcgta cgacatcacc tgccgtcgtc ttcgcctcga acgccgagtt actcgacaag tcccgtgacc cctgtgacgc cgacgcgcgg ttacagtggt actaagatac acacgcaaaa taaatcgcca ccagcttctt ctctgcctca catgtccatc gaggactgcc gcggctttca tgaggtaaag gcccttctcc aagagctggg actcgacatt ccgatcgtgc accatgacga gaaggccacc gtcgaggagg tgctggacct cctgcacaaa atgggcatcc acgctgcggg caccaagacg ctcttcctca agagcaagaa gggcgaactg gtgatggcga cggcgttcaa gtccaccccg acggacctca agttcattca gaaggtcacc aacgccaagg acctccgctt tgcttccagc gaggtgctgc aggagtgcct cgccgtcgtg cagggctgcg tcacaccgtt tgccctcatc aacaatatcg agaagcgtcc cattacgctc ctcatagacc gctccctgga ggccagcacg ctgccgctcg ccttctgcct gtgccgtaac gattacactg ccgtcataac cttcgaggag ctcaagaagt acctcgagaa gctcgggcat gcgtacaagc tggtggactt tggtgcggct tcggcggacg ctgcggtgac gggcggcgct gctgctcctg ctccgaagcc gaagaaggcg cccgcggacg ctgctgctgc tgctgctgcg cctgcgtctg cacagtctgg cgagacgaag ctgggcatcg cggcgaagcg cgaggagaac ttctctgcgt ggtacattga tgtgatcacg aaggcggaga tgatcgagta ctacgacgtg tccgggtgct acattattcg tccgtgggcg tactacgtgt ggaagtgcgt gcagcgcttc cttggtggga agatcgagaa gcttggcgtg gaggactgct acttcccgat gttcgtgtcg cgcaactgcc tggagcggga gaaggaccac atcgagggct ttgcgccgga ggttgcatgg gtgacgcgcg ccggcgacac ggagctggag cagccggttg ccgtgcggcc gacgagcgag acggtgatgt acccgtacta cgcgaagtgg atccggtcgc accgcgacct gcccgtgcgg ctgaacatgt ggaacaacgt gatccggtgg gagttctcgc acccgacgcc gttcattcgg actcgcgagt tcctgtggca ggaggggcac tgtgcgtggg cgaaggcgga ggagtgcgcg aaggaggtgc tggacatcct ggagtgctat gcatcggtgt acgagcagct gctggcggtg ccggtggtgc gcgggcgcaa gacggagaag gagaagttcg ctggcgggga ctacacgacg acggtggaga cgttcatcga ggctgttggg cgcgggtgcc agggcgcgac gagccacaac ctggggcaga acttcggcaa gatgttcgac atccgcttcc aggacccgga gaacaacgag cagacgctga tcccgtggca gaacagctgg gggctgtcga cgcgcgtgat cggcgtgatg atcatggtgc acggcgacaa ccgcggcatg gtgatgccgc cgcgcgttgc gtcgacgcag gtgatcatca tccctgtggg gatcacgaag gacacgacgg aggaggcgcg gcaggagctg cttgcaagtt gccggcggct ggagagcgag ctgtgcgagg gtggcgtgcg cgcgaagtgc gacctgcgcg acaactacag ccctgggtgg cggttcaacc actgggaggt gaagggcgtg ccactgcgcg tggagcttgg gccgagggag cttgcggagc ggtcgcttgc ggttgctgtg cggcacagcg gcgcgaggca ctcggttgtg tgggacgcgc agacgccgac ggcggtagcc gcactgctgg aggatgtgca tgcacagatg tacgcgcgcg cgaaggagac gatggagacg catcgcgtgc gtgtgacgga gtgggcggag tttgtgccga cgctgaaccg gaagtgcttg atccttgcgc cgtggtgcgg cgcgatggag tgcgaggacc aggtaaagaa ggacagcgcg gaggagtcga aggctgcgca ggcgcaggag acgcgcgagg acgcgcgtgc gccgagcatg ggtgcgaaga cgctgtgcat cccgtttgag cagccggagg accccgcaga gggtcacggg tgcatctgca agggctgcac gaagccggcg acgacgtggg tgctgtttgg ccgcagctac tga

Details for LeinA.18681.a.B2.GE42606

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	89kDa
OBSERVED MW:	89kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLS SSRASHRSIT RHLGVRHHLP SSSPRTPSYS TSPVTPVTPT RGYSGTKIHT QNKSPPASSL PHMSIEDCRG FHEVKALLQE LGLDIPIVHH DEKATVEEVL DLLHKMGIHA AGTKTLFLKS KKGELVMATA FKSTPTDLKF IQKVTNAKDL RFASSEVLQE CLAVVQGCVT PFALINNIEK RPITLLIDRS LEASTLPLAF CLCRNDYTAV ITFEELKKYL EKLGHAYKLV DFGAASADAA VTGGAAAPAP KPKKAPADAA AAAAAPASAQ SGETKLGIAA KREENFSAWY IDVITKAEMI EYYDVSGCYI IRPWAYYVWK CVQRFLGGKI EKLGVEDCYF PMFVSRNCLE REKDHIEGFA PEVAWVTRAG DTELEQPVAV RPTSETVMYP YYAKWIRSHR DLPVRLNMWN NVIRWEFSHP TPFIRTREFL WQEGHCAWAK AEECAKEVLD ILECYASVYE QLLAVPVVRG RKTEKEKFAG GDYTTTVETF IEAVGRGCQG ATSHNLGQNF GKMFDIRFQD PENNEQTLIP WQNSWGLSTR VIGVMIMVHG DNRGMVMPPR VASTQVIIIP VGITKDTTEE ARQELLASCR RLESELCEGG VRAKCDLRDN YSPGWRFNHW EVKGVPLRVE LGPRELAERS LAVAVRHSGA RHSVVWDAQT PTAVAALLED VHAQMYARAK ETMETHRVRV TEWAEFVPTL NRKCLILAPW CGAMECEDQV KKDSAEESKA AQAQETREDA RAPSMGAKTL CIPFEQPEDP AEGHGCICKG CTKPATTWVL FGRSY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctatcct ccagtcgggc ttcgcatcga tcgatcacgc gccacttggg cgtacgacat cacctgccgt cgtcttcgcc tcgaacgccg agttactcga caagtcccgt gacccctgtg acgccgacgc gcggttacag tggtactaag atacacacgc aaaataaatc gccaccagct tcttctctgc ctcacatgtc catcgaggac tgccgcggct ttcatgaggt aaaggccctt ctccaagagc tgggactcga cattccgatc gtgcaccatg acgagaaggc caccgtcgag gaggtgctgg acctcctgca caaaatgggc atccacgctg cgggcaccaa gacgctcttc ctcaagagca agaagggcga actggtgatg gcgacggcgt tcaagtccac cccgacggac ctcaagttca ttcagaaggt caccaacgcc aaggacctcc gctttgcttc cagcgaggtg ctgcaggagt gcctcgccgt cgtgcagggc tgcgtcacac cgtttgccct catcaacaat atcgagaagc gtcccattac gctcctcata gaccgctccc tggaggccag cacgctgccg ctcgccttct gcctgtgccg taacgattac actgccgtca taaccttcga ggagctcaag aagtacctcg agaagctcgg gcatgcgtac aagctggtgg actttggtgc ggcttcggcg gacgctgcgg tgacgggcgg cgctgctgct cctgctccga agccgaagaa ggcgcccgcg gacgctgctg ctgctgctgc tgcgcctgcg tctgcacagt ctggcgagac gaagctgggc atcgcggcga agcgcgagga gaacttctct gcgtggtaca ttgatgtgat cacgaaggcg gagatgatcg agtactacga cgtgtccggg tgctacatta ttcgtccgtg ggcgtactac gtgtggaagt gcgtgcagcg cttccttggt gggaagatcg agaagcttgg cgtggaggac tgctacttcc cgatgttcgt gtcgcgcaac tgcctggagc gggagaagga ccacatcgag ggctttgcgc cggaggttgc atgggtgacg cgcgccggcg acacggagct ggagcagccg gttgccgtgc ggccgacgag cgagacggtg atgtacccgt actacgcgaa gtggatccgg tcgcaccgcg acctgcccgt gcggctgaac atgtggaaca acgtgatccg gtgggagttc tcgcacccga cgccgttcat tcggactcgc gagttcctgt ggcaggaggg gcactgtgcg tgggcgaagg cggaggagtg cgcgaaggag gtgctggaca tcctggagtg ctatgcatcg gtgtacgagc agctgctggc ggtgccggtg gtgcgcgggc gcaagacgga gaaggagaag ttcgctggcg gggactacac gacgacggtg gagacgttca tcgaggctgt tgggcgcggg tgccagggcg cgacgagcca caacctgggg cagaacttcg gcaagatgtt cgacatccgc ttccaggacc cggagaacaa cgagcagacg ctgatcccgt ggcagaacag ctgggggctg tcgacgcgcg tgatcggcgt gatgatcatg gtgcacggcg acaaccgcgg catggtgatg ccgccgcgcg ttgcgtcgac gcaggtgatc atcatccctg tggggatcac gaaggacacg acggaggagg cgcggcagga gctgcttgca agttgccggc ggctggagag cgagctgtgc gagggtggcg tgcgcgcgaa gtgcgacctg cgcgacaact acagccctgg gtggcggttc aaccactggg aggtgaaggg cgtgccactg cgcgtggagc ttgggccgag ggagcttgcg gagcggtcgc ttgcggttgc tgtgcggcac agcggcgcga ggcactcggt tgtgtgggac gcgcagacgc cgacggcggt agccgcactg ctggaggatg tgcatgcaca gatgtacgcg cgcgcgaagg agacgatgga gacgcatcgc gtgcgtgtga cggagtgggc ggagtttgtg ccgacgctga accggaagtg cttgatcctt gcgccgtggt gcggcgcgat ggagtgcgag gaccaggtaa agaaggacag cgcggaggag tcgaaggctg cgcaggcgca ggagacgcgc gaggacgcgc gtgcgccgag catgggtgcg aagacgctgt gcatcccgtt tgagcagccg gaggaccccg cagagggtca cgggtgcatc tgcaagggct gcacgaagcc ggcgacgacg tgggtgctgt ttggccgcag ctactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LeinA.18681.a.B2.PW38683

PURIFICATION DATe:	8/12/2019
CONCENTRATION:	25.5mg/ml
OBSERVED MW:	89kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTATCCT CCAGTCGGGC TTCGCATCGA TCGATCACGC GCCACTTGGG CGTACGACAT CACCTGCCGT CGTCTTCGCC TCGAACGCCG AGTTACTCGA CAAGTCCCGT GACCCCTGTG ACGCCGACGC GCGGTTACAG TGGTACTAAG ATACACACGC AAAATAAATC GCCACCAGCT TCTTCTCTGC CTCACATGTC CATCGAGGAC TGCCGCGGCT TTCATGAGGT AAAGGCCCTT CTCCAAGAGC TGGGACTCGA CATTCCGATC GTGCACCATG ACGAGAAGGC CACCGTCGAG GAGGTGCTGG ACCTCCTGCA CAAAATGGGC ATCCACGCTG CGGGCACCAA GACGCTCTTC CTCAAGAGCA AGAAGGGCGA ACTGGTGATG GCGACGGCGT TCAAGTCCAC CCCGACGGAC CTCAAGTTCA TTCAGAAGGT CACCAACGCC AAGGACCTCC GCTTTGCTTC CAGCGAGGTG CTGCAGGAGT GCCTCGCCGT CGTGCAGGGC TGCGTCACAC CGTTTGCCCT CATCAACAAT ATCGAGAAGC GTCCCATTAC GCTCCTCATA GACCGCTCCC TGGAGGCCAG CACGCTGCCG CTCGCCTTCT GCCTGTGCCG TAACGATTAC ACTGCCGTCA TAACCTTCGA GGAGCTCAAG AAGTACCTCG AGAAGCTCGG GCATGCGTAC AAGCTGGTGG ACTTTGGTGC GGCTTCGGCG GACGCTGCGG TGACGGGCGG CGCTGCTGCT CCTGCTCCGA AGCCGAAGAA GGCGCCCGCG GACGCTGCTG CTGCTGCTGC TGCGCCTGCG TCTGCACAGT CTGGCGAGAC GAAGCTGGGC ATCGCGGCGA AGCGCGAGGA GAACTTCTCT GCGTGGTACA TTGATGTGAT CACGAAGGCG GAGATGATCG AGTACTACGA CGTGTCCGGG TGCTACATTA TTCGTCCGTG GGCGTACTAC GTGTGGAAGT GCGTGCAGCG CTTCCTTGGT GGGAAGATCG AGAAGCTTGG CGTGGAGGAC TGCTACTTCC CGATGTTCGT GTCGCGCAAC TGCCTGGAGC GGGAGAAGGA CCACATCGAG GGCTTTGCGC CGGAGGTTGC ATGGGTGACG CGCGCCGGCG ACACGGAGCT GGAGCAGCCG GTTGCCGTGC GGCCGACGAG CGAGACGGTG ATGTACCCGT ACTACGCGAA GTGGATCCGG TCGCACCGCG ACCTGCCCGT GCGGCTGAAC ATGTGGAACA ACGTGATCCG GTGGGAGTTC TCGCACCCGA CGCCGTTCAT TCGGACTCGC GAGTTCCTGT GGCAGGAGGG GCACTGTGCG TGGGCGAAGG CGGAGGAGTG CGCGAAGGAG GTGCTGGACA TCCTGGAGTG CTATGCATCG GTGTACGAGC AGCTGCTGGC GGTGCCGGTG GTGCGCGGGC GCAAGACGGA GAAGGAGAAG TTCGCTGGCG GGGACTACAC GACGACGGTG GAGACGTTCA TCGAGGCTGT TGGGCGCGGG TGCCAGGGCG CGACGAGCCA CAACCTGGGG CAGAACTTCG GCAAGATGTT CGACATCCGC TTCCAGGACC CGGAGAACAA CGAGCAGACG CTGATCCCGT GGCAGAACAG CTGGGGGCTG TCGACGCGCG TGATCGGCGT GATGATCATG GTGCACGGCG ACAACCGCGG CATGGTGATG CCGCCGCGCG TTGCGTCGAC GCAGGTGATC ATCATCCCTG TGGGGATCAC GAAGGACACG ACGGAGGAGG CGCGGCAGGA GCTGCTTGCA AGTTGCCGGC GGCTGGAGAG CGAGCTGTGC GAGGGTGGCG TGCGCGCGAA GTGCGACCTG CGCGACAACT ACAGCCCTGG GTGGCGGTTC AACCACTGGG AGGTGAAGGG CGTGCCACTG CGCGTGGAGC TTGGGCCGAG GGAGCTTGCG GAGCGGTCGC TTGCGGTTGC TGTGCGGCAC AGCGGCGCGA GGCACTCGGT TGTGTGGGAC GCGCAGACGC CGACGGCGGT AGCCGCACTG CTGGAGGATG TGCATGCACA GATGTACGCG CGCGCGAAGG AGACGATGGA GACGCATCGC GTGCGTGTGA CGGAGTGGGC GGAGTTTGTG CCGACGCTGA ACCGGAAGTG CTTGATCCTT GCGCCGTGGT GCGGCGCGAT GGAGTGCGAG GACCAGGTAA AGAAGGACAG CGCGGAGGAG TCGAAGGCTG CGCAGGCGCA GGAGACGCGC GAGGACGCGC GTGCGCCGAG CATGGGTGCG AAGACGCTGT GCATCCCGTT TGAGCAGCCG GAGGACCCCG CAGAGGGTCA CGGGTGCATC TGCAAGGGCT GCACGAAGCC GGCGACGACG TGGGTGCTGT TTGGCCGCAG CTACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT