LeinA.18419.a

Methylene-tetrahydrofolate dehydrogenase-like protein (DHCH2)

CENTER ID: LeinA.18419.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18419.a.B2.GE35989 soluble domain 33 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LinJ.22.0220
RefSeq: XP_001465553.1
UniProt: A4HZR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGSFSVSAS FALTSASSMA LSTLTLRRSR SAELLSGVPI SKELRQRIRT STSVLRRPPC LVVVLVGDRA DAIRYVNHKR RAAAECGIEV HLVHLSATIQ QVELHRTLAS VNDDVEVDAV LLQLPLPPHL RTRPALLHIH PGKDVDGLHP LNAGSLCLQD QRSRLHTLVA ASSAASASLV DQQAQGMIEE MLLPAATSGG DSAMRHRFHE RNIFVPCTAL AIRTVLFSYL GRTENFAHLL PAAHSNDMSA VEGPSTLPSP SLSATAGIVS PSSPEGKQFP RSSASTQAPP RRDLHAVIVN NSRVVGVPTA ALLQCAGSFA VTLCSRSNSL DTIRHVAQQA DVLITAYGAA NVFDRSFVRE GAIVIDAAVN ELPEMTCQKE AAAETGGSAR KKGICGDVDI NSVSAVAAAI TKTPGGVGPL TASHLMFNVL KAYQLRHDNE RHYNNIYTEF LKMYGTHEKV RGHAPPLGMP FMAAP
NT Sequence
atgtgcgggt ctttttctgt gagtgcgtct ttcgctctta ctagcgcgag tagcatggcc ctctccactc tgacgcttcg gcggtcgcgc tcagcagagt tgctgtctgg cgtgccgatc agcaaggagc tacggcagcg aatccgcacc agcacgtcag tcctccgtcg gccaccatgc ctggttgtgg tgctcgtcgg tgaccgtgcc gatgcgatcc gctacgtcaa ccacaagagg cgcgccgccg cggagtgcgg cattgaagtg catctcgttc atctttccgc taccattcag caggttgagc ttcacaggac cctggcttcc gtcaacgacg acgtcgaggt cgacgccgtc cttctccagc tgccgctgcc acctcatctg cgtactcgac cggcactgct ccacatccac ccaggcaagg acgtggatgg cctccacccc ttgaacgccg gaagcctctg cctgcaggat cagaggtcgc gcctgcatac acttgtcgcg gcatcctcgg cagcatcagc gtcgctcgtc gaccagcagg cgcagggaat gattgaggag atgctgttgc cggctgccac cagcggcggc gacagcgcca tgcgtcaccg tttccatgag cgcaacatct ttgtgccgtg caccgccctc gccatccgca cagtgctctt ctcctatctc ggccgcacgg aaaacttcgc gcacttgttg ccggcagccc acagcaacga catgtctgca gtggagggtc catcaacgct tccgtcccca tcgttgtcgg caactgccgg catcgtttcc ccgtcgtcgc cggaaggcaa gcaattccca cggagcagcg cgagtaccca agcacctccc cgccgcgacc tgcacgccgt gatcgtgaac aacagcaggg tggtgggggt gccgacggcg gcgctgctgc agtgcgcagg cagctttgcc gtcacgttgt gcagtcgcag taactcgctc gacaccattc gacatgtcgc gcaacaggcg gacgtgctca tcacggctta tggagcggcg aatgtgttcg accggtcgtt tgttcgagag ggggccatcg tgatcgacgc cgccgtcaac gagttaccgg agatgacgtg ccagaaggag gcagcggcag agacaggcgg cagcgcgcga aagaagggca tctgcggcga cgtcgacata aattcggtgt ccgctgttgc cgcggcaatc acgaagacac caggcggtgt tggccctctt acggcgtcgc acctgatgtt caacgtgttg aaggcgtatc agcttcgaca cgacaacgag cgtcactaca acaacatcta cacagagttc ctgaagatgt acggcacgca cgaaaaggtg cggggccatg cgccgcctct agggatgcca ttcatggctg ccccatga
Details for LeinA.18419.a.B2.GE35989
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 83
Validated AA Sequence
MAHHHHHHME LLSGVPISKE LRQRIRTSTS VLRRPPCLVV VLVGDRADAI RYVNHKRRAA AECGIEVHLV HLSATIQQVE LHRTLASVND DVEVDAVLLQ LPLPPHLRTR PALLHIHPGK DVDGLHPLNA GSLCLQDQRS RLHTLVAASS AASASLVDQQ AQGMIEEMLL PAATSGGDSA MRHRFHERNI FVPCTALAIR TVLFSYLGRT ENFAHLLPAA HSNDMSAVEG PSTLPSXSLS ATAGIVSPSS PEGKQFPRSS ASTQAPPRRD LHAVIVNNSR XVGVPTAALL QCAXALPSRC AVAVTRSTPF DMSRXXXXAH HXXWXXXVXX XXXXXXGXXX IXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatggagttg ctgtctggcg tgccgatcag caaggagcta cggcagcgaa tccgcaccag cacgtcagtc ctccgtcgac caccatgcct ggttgtggtg ctcgtcggtg accgtgccga tgcgatccgc tacgtcaacc acaagaggcg cgccgccgcg gagtgcggca ttgaagtgca tctcgttcat ctttccgcta ccattcagca ggttgagctt cacaggaccc tggcttccgt caacgacgac gtcgaggtcg acgccgtcct tctccagctg ccgctgccac ctcatctgcg tactcgaccg gcactgctcc acatccaccc aggcaaggac gtggatggcc tccacccctt gaacgccgga agcctctgcc tgcaggatca gaggtcgcgc ctgcatacac ttgtcgcggc atcctcggca gcatcagcgt cgctcgtcga ccagcaggcg cagggaatga ttgaggagat gctgttgccg gctgccacca gcggcggcga cagcgccatg cgtcaccgtt tccatgagcg caacatcttt gtgccgtgca ccgccctcgc catccgcaca gtgctcttct cctatctcgg ccgcacggaa aacttcgcgc acttgttgcc ggcagcccac agcaacgaca tgtctgcagt ggagggtcca tcaacgcttc cgtccncatc gttgtcggca actgccggca tcgtttcccc gtcgtcgccg gaaggcaagc aattcccacg gagcagcgcg agtacccaag cacctccccg ccgcgacctg cacgccgtga tcgtgaacaa cagcaggntg gtgggggtgc cgacggcggc gctgctgcag tgcgcnnnag ctttgccgtc acgttgtgca gtcgcagtaa ctcgctcgac accattcgac atgtcgcgca ncnnnngncn tgctcatcac gnnnnatggn ncnnnnnngt gntcnancng nncnttngtc nannngggnn nntcnnnatc gannnnnnnn annnnnnnnn nnnnnannnn nnnnnnnnan ngnnnnnnnn nngnnnncnn nnnnnnnnan nnnnnnnnnn nnnnnncnnn nncnnnnnnn nnnnnnnccn nnnnng
Expected Protein Sequence
MAHHHHHHME LLSGVPISKE LRQRIRTSTS VLRRPPCLVV VLVGDRADAI RYVNHKRRAA AECGIEVHLV HLSATIQQVE LHRTLASVND DVEVDAVLLQ LPLPPHLRTR PALLHIHPGK DVDGLHPLNA GSLCLQDQRS RLHTLVAASS AASASLVDQQ AQGMIEEMLL PAATSGGDSA MRHRFHERNI FVPCTALAIR TVLFSYLGRT ENFAHLLPAA HSNDMSAVEG PSTLPSPSLS ATAGIVSPSS PEGKQFPRSS ASTQAPPRRD LHAVIVNNSR VVGVPTAALL QCAGSFAVTL CSRSNSLDTI RHVAQQADVL ITAYGAANVF DRSFVREGAI VIDAAVNELP EMTCQKEAAA ETGGSARKKG ICGDVDINSV SAVAAAITKT PGGVGPLTAS HLMFNVLKAY QLRHDNERHY NNIYTEFLKM YGTHEKVRGH APPLGMPFMA AP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagt tgctgtctgg cgtgccgatc agcaaggagc tacggcagcg aatccgcacc agcacgtcag tcctccgtcg gccaccatgc ctggttgtgg tgctcgtcgg tgaccgtgcc gatgcgatcc gctacgtcaa ccacaagagg cgcgccgccg cggagtgcgg cattgaagtg catctcgttc atctttccgc taccattcag caggttgagc ttcacaggac cctggcttcc gtcaacgacg acgtcgaggt cgacgccgtc cttctccagc tgccgctgcc acctcatctg cgtactcgac cggcactgct ccacatccac ccaggcaagg acgtggatgg cctccacccc ttgaacgccg gaagcctctg cctgcaggat cagaggtcgc gcctgcatac acttgtcgcg gcatcctcgg cagcatcagc gtcgctcgtc gaccagcagg cgcagggaat gattgaggag atgctgttgc cggctgccac cagcggcggc gacagcgcca tgcgtcaccg tttccatgag cgcaacatct ttgtgccgtg caccgccctc gccatccgca cagtgctctt ctcctatctc ggccgcacgg aaaacttcgc gcacttgttg ccggcagccc acagcaacga catgtctgca gtggagggtc catcaacgct tccgtcccca tcgttgtcgg caactgccgg catcgtttcc ccgtcgtcgc cggaaggcaa gcaattccca cggagcagcg cgagtaccca agcacctccc cgccgcgacc tgcacgccgt gatcgtgaac aacagcaggg tggtgggggt gccgacggcg gcgctgctgc agtgcgcagg cagctttgcc gtcacgttgt gcagtcgcag taactcgctc gacaccattc gacatgtcgc gcaacaggcg gacgtgctca tcacggctta tggagcggcg aatgtgttcg accggtcgtt tgttcgagag ggggccatcg tgatcgacgc cgccgtcaac gagttaccgg agatgacgtg ccagaaggag gcagcggcag agacaggcgg cagcgcgcga aagaagggca tctgcggcga cgtcgacata aattcggtgt ccgctgttgc cgcggcaatc acgaagacac caggcggtgt tggccctctt acggcgtcgc acctgatgtt caacgtgttg aaggcgtatc agcttcgaca cgacaacgag cgtcactaca acaacatcta cacagagttc ctgaagatgt acggcacgca cgaaaaggtg cggggccatg cgccgcctct agggatgcca ttcatggctg ccccatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat