SSGCID: Target Page

LeinA.18297.a

Uncharacterized protein

CENTER ID:	LeinA.18297.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18297.a.B1.GE37539	full length		1	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.18297.a.B1.PS01993	full length		1	339	unavailable

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LinJ.27.1630
RefSeq:	XP_001466397.1
UniProt:	A4I2W4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLCASIPAT VDTDTNTRAL YLDDLDKCMK NFNSALASTM NAYQNLLTAF DQVAQVYGNV AQECGDEVRK PVGEFRDGMR DLKDKGGFET FNHEIHVGTI AVMEPVKLDL KKALKSLKFL RLKQKEYDTV RYELEQTEKS YAKKNKPLVA SNSYKKTLAK RDKVKASYDA SREAFGDEMN ALQATTKSVL LQSLNNYLHC TAAFCGQLEA TMDGYRTDVD HHGSTTFAND QMDKLKEKAE AESMARRAQR KNEASNPFQT GNDVTSNNSH RNLPSVDDGL YQKKDSLVDT ESANRYPNAE LAPPMQNGGD DFTLREQYEN EAPGDADAVN PLVDANDDE

NT Sequence

atgccgctct gcgccagcat ccccgcgacg gtcgacaccg acacgaacac ccgcgctctc tacctggatg atttggacaa gtgcatgaag aacttcaaca gtgccctcgc gtccaccatg aacgcgtacc aaaacctcct caccgcgttt gatcaggtgg cgcaggtgta cggcaacgtc gcccaggagt gcggggatga ggtgcgcaag ccggttggcg aattccgcga cgggatgcgc gatctcaagg acaaaggtgg cttcgagacg ttcaaccacg agatccacgt gggcacgatt gctgtaatgg agccggtgaa attggacctg aagaaggcgc tcaagtcgct caagttcctg aggctgaagc agaaggagta cgacaccgtt cgctacgagc tggagcagac cgagaagtcg tacgcgaaga agaacaagcc cttggtagcg agcaacagct acaagaagac gctcgcaaag cgagataagg tgaaggcgtc gtacgatgcg agtcgcgagg cctttggcga cgagatgaac gcactgcagg ctacgaccaa gtccgttctg ctccagtccc tcaacaacta cctccactgc acggccgctt tttgtggaca actggaggca accatggacg gctaccgcac cgacgtggac caccatggca gcaccacgtt tgcaaacgac caaatggaca agctgaagga gaaagccgaa gccgagtcga tggcgcggcg cgcgcagcgc aagaacgagg cgtcgaaccc gttccagact ggaaacgatg tcacatcgaa caacagccac cgcaacttgc cttcggttga tgatggcctc taccaaaaga aggattcgct ggtggacact gagagcgcca accgctaccc caacgctgaa cttgcgccgc cgatgcagaa cggcggtgac gacttcacgc tgagggagca gtatgagaac gaggcgcccg gcgatgccga tgccgtgaac ccgctcgttg acgcaaacga cgacgagtga

Details for LeinA.18297.a.B1.GE37539

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatgccgct ctgcgccagc atccccgcga cggtcgacac cgacacgaac acccgcgctc tctacctgga tgatttggac aagtgcatga agaacttcaa cagtgccctc gcgtccacca tgaacgcgta ccaaaacctc ctcaccgcgt ttgatcaggt ggcgcaggtg tacggcaacg tcgcccagga gtgcggggat gaggtgcgca agccggttgg cgaattccgc gacgggatgc gcgatctcaa ggacaaaggt ggcttcgaga cgttcaacca cgagatccac gtgggcacga ttgctgtaat ggagccggtg aaattggacc tgaagaaggc gctcaagtcg ctcaagttcc tgaggctgaa gcagaaggag tacgacaccg ttcgctacga gctggagcag accgagaagt cgtacgcgaa gaagaacaag cccttggtag cgagcaacag ctacaagaag acgctcgcaa agcgagataa ggtgaaggcg tcgtacgatg cgagtcgcga ggcctttggc gacgagatga acgcactgca ggctacgacc aagtccgttc tgctccagtc cctcaacaac tacctccact gcacggccgc tttttgtgga caactggagg caaccatgga cggctaccgc accgacgtgg accaccatgg cagcaccacg tttgcaaacg accaaatgga caagctgaag gagaaagccg aagccgagtc gatggcgcgg cgcgcgcagc gcaagaacga ggcgtcgaac ccgttccaga ctggaaacga tgtcacatcg aacaacagcc accgcaactt gccttcggtt gatgatggcc tctaccaaaa gaaggattcg ctggtggaca ctgagagcgc caaccgctac cccaacgctg aacttgcgcc gccgatgcag aacggcggtg acgacttcac gctgagggag cagtatgaga acgaggcgcc cggcgatgcc gatgccgtga acccgctcgt tgacgcaaac gacgacgagt aagtgagta

Expected Protein Sequence

MAHHHHHHMP LCASIPATVD TDTNTRALYL DDLDKCMKNF NSALASTMNA YQNLLTAFDQ VAQVYGNVAQ ECGDEVRKPV GEFRDGMRDL KDKGGFETFN HEIHVGTIAV MEPVKLDLKK ALKSLKFLRL KQKEYDTVRY ELEQTEKSYA KKNKPLVASN SYKKTLAKRD KVKASYDASR EAFGDEMNAL QATTKSVLLQ SLNNYLHCTA AFCGQLEATM DGYRTDVDHH GSTTFANDQM DKLKEKAEAE SMARRAQRKN EASNPFQTGN DVTSNNSHRN LPSVDDGLYQ KKDSLVDTES ANRYPNAELA PPMQNGGDDF TLREQYENEA PGDADAVNPL VDANDDE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc tctgcgccag catccccgcg acggtcgaca ccgacacgaa cacccgcgct ctctacctgg atgatttgga caagtgcatg aagaacttca acagtgccct cgcgtccacc atgaacgcgt accaaaacct cctcaccgcg tttgatcagg tggcgcaggt gtacggcaac gtcgcccagg agtgcgggga tgaggtgcgc aagccggttg gcgaattccg cgacgggatg cgcgatctca aggacaaagg tggcttcgag acgttcaacc acgagatcca cgtgggcacg attgctgtaa tggagccggt gaaattggac ctgaagaagg cgctcaagtc gctcaagttc ctgaggctga agcagaagga gtacgacacc gttcgctacg agctggagca gaccgagaag tcgtacgcga agaagaacaa gcccttggta gcgagcaaca gctacaagaa gacgctcgca aagcgagata aggtgaaggc gtcgtacgat gcgagtcgcg aggcctttgg cgacgagatg aacgcactgc aggctacgac caagtccgtt ctgctccagt ccctcaacaa ctacctccac tgcacggccg ctttttgtgg acaactggag gcaaccatgg acggctaccg caccgacgtg gaccaccatg gcagcaccac gtttgcaaac gaccaaatgg acaagctgaa ggagaaagcc gaagccgagt cgatggcgcg gcgcgcgcag cgcaagaacg aggcgtcgaa cccgttccag actggaaacg atgtcacatc gaacaacagc caccgcaact tgccttcggt tgatgatggc ctctaccaaa agaaggattc gctggtggac actgagagcg ccaaccgcta ccccaacgct gaacttgcgc cgccgatgca gaacggcggt gacgacttca cgctgaggga gcagtatgag aacgaggcgc ccggcgatgc cgatgccgtg aacccgctcg ttgacgcaaa cgacgacgag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for LeinA.18297.a.B1.PS01993

PURIFICATION DATe:	2/12/2014
CONCENTRATION:	21.14mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGC TCTGCGCCAG CATCCCCGCG ACGGTCGACA CCGACACGAA CACCCGCGCT CTCTACCTGG ATGATTTGGA CAAGTGCATG AAGAACTTCA ACAGTGCCCT CGCGTCCACC ATGAACGCGT ACCAAAACCT CCTCACCGCG TTTGATCAGG TGGCGCAGGT GTACGGCAAC GTCGCCCAGG AGTGCGGGGA TGAGGTGCGC AAGCCGGTTG GCGAATTCCG CGACGGGATG CGCGATCTCA AGGACAAAGG TGGCTTCGAG ACGTTCAACC ACGAGATCCA CGTGGGCACG ATTGCTGTAA TGGAGCCGGT GAAATTGGAC CTGAAGAAGG CGCTCAAGTC GCTCAAGTTC CTGAGGCTGA AGCAGAAGGA GTACGACACC GTTCGCTACG AGCTGGAGCA GACCGAGAAG TCGTACGCGA AGAAGAACAA GCCCTTGGTA GCGAGCAACA GCTACAAGAA GACGCTCGCA AAGCGAGATA AGGTGAAGGC GTCGTACGAT GCGAGTCGCG AGGCCTTTGG CGACGAGATG AACGCACTGC AGGCTACGAC CAAGTCCGTT CTGCTCCAGT CCCTCAACAA CTACCTCCAC TGCACGGCCG CTTTTTGTGG ACAACTGGAG GCAACCATGG ACGGCTACCG CACCGACGTG GACCACCATG GCAGCACCAC GTTTGCAAAC GACCAAATGG ACAAGCTGAA GGAGAAAGCC GAAGCCGAGT CGATGGCGCG GCGCGCGCAG CGCAAGAACG AGGCGTCGAA CCCGTTCCAG ACTGGAAACG ATGTCACATC GAACAACAGC CACCGCAACT TGCCTTCGGT TGATGATGGC CTCTACCAAA AGAAGGATTC GCTGGTGGAC ACTGAGAGCG CCAACCGCTA CCCCAACGCT GAACTTGCGC CGCCGATGCA GAACGGCGGT GACGACTTCA CGCTGAGGGA GCAGTATGAG AACGAGGCGC CCGGCGATGC CGATGCCGTG AACCCGCTCG TTGACGCAAA CGACGACGAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT