LeinA.18295.a

arginyl-tRNA synthetase, putative

CENTER ID: LeinA.18295.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18295.a.B1.GE42531 full length 1 692
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.27.1230
UniProt: A4I2T4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCAAAATVNV ELALKEIVKA TIAKAFPLVP AQEPLITLGK VSEYQCNNAM GLVKVLSQQQ QPIKMSPQMV GEELKKNLVG NDIIDEFEPT PKGFINVSIR KEWVARMVKE VLKQGIRPPV VTKQRVLVDF SAPNIAKEMH VGHLRSTIIG ESISRLLEFC QHDVARINHI GDWGTAFGML ILYIKRNFPD YTTNPPDISD LTDFYRAAKK CFDEDQEFKE KARLEVVKLQ ALEEDSIQAW KYICDVSREE FDKVYTRLGA TIEERGESFY NPLIPKVLDL LEEAGQIEVS DGAKLIVSKE ARKLDSLDAR DMTKLASQHL ALVSRDGPSY HPNLLAAMKK AGILTGTEGE ESVALSKKEV KPWNKFDIRV DLDKLMAQLA PLYKKQLDPL FLEVFEAAGI VKGDSILVPR FSFPLMVVKS DGGYTYDTTD VTATYHRFVI EKMNRVIYCT DLGQYEHFRM CMQTAKDMGW MEHATWDHAG FGLVTGADGK KIKTRSGETV KLKDLMDEAV ERSLAILKER EAGERSQGHS EEEMQKLSNI IGIGAIKYFD LKQTRTGDYA FSYDKMLDMS GNTAVFLLYQ YARICSIKRK AGIADEELLE ITDISLETPQ EKSLALCALR FQTVILKTVE DLFPHHLADF AYELMTNFSN FFQNCRVLDD PLQNSRLCLV ELTRITLKKT LELLNIETAE RI
NT Sequence
atgtgcgccg ctgccgctac cgtgaatgtg gagctggcgc tgaaggagat tgtcaaggcg accatcgcca aggccttccc gctcgtcccg gcccaggagc ctctcatcac ccttggcaag gtgagcgagt atcagtgcaa caatgccatg gggctggtga aggtgctctc tcagcagcag cagccaataa agatgagccc gcagatggtc ggcgaggagc tcaaaaagaa cttggtcggc aatgatatca tcgacgagtt cgaacccacg cccaagggtt tcattaatgt cagcatccgc aaggaatggg tggctaggat ggtgaaggag gtgctgaaac agggcatccg cccacccgtc gtgacgaagc agcgcgtgct cgtcgacttt tcagcgccga acattgccaa ggagatgcat gtcggacatc tgcgctccac catcatcggc gagtcgatca gccgcctgct ggagttctgc cagcatgacg ttgcacgcat caaccacatc ggcgactggg gtacggcgtt tggcatgctc attctgtaca tcaagcgcaa cttccccgac tacaccacga acccgccgga catctctgac ctcaccgact tctaccgcgc ggcgaagaag tgcttcgacg aggaccagga gttcaaggag aaggcccgcc tcgaggtggt gaagctgcag gcgctggagg aggattctat ccaggcgtgg aagtacatct gcgacgtgtc gcgcgaggag ttcgacaagg tttacacccg cctcggcgcc accatcgagg agcgcggtga gagcttctac aacccgctta ttccaaaggt gctcgatctg ctggaggagg cgggacagat cgaggtgagt gacggcgcga agctcatcgt cagcaaggag gcgcgcaaac tggatagcct cgatgcgcgt gacatgacaa agctggcgag tcagcacctc gcgctcgtct cgcgcgatgg tccgtcgtac cacccgaacc tgcttgccgc catgaagaag gctggcatct tgaccgggac tgagggtgag gagtcggtgg cgctgtcgaa gaaggaggtg aagccgtgga acaagtttga tatccgcgtg gacctggaca agctgatggc ccagctcgca cccctgtaca agaagcagct cgatcctctc ttcctggagg tgttcgaggc tgcggggatt gtcaagggcg acagcatcct tgtcccccgt ttctccttcc cgctgatggt ggtgaaaagt gatggcggct acacctacga cacgacagat gtcacggcca cgtaccaccg cttcgtcatc gagaagatga accgcgtcat ctactgcacc gatttgggcc agtacgagca cttccgcatg tgcatgcaga cggcaaagga catgggctgg atggaacatg ccacatggga ccacgccggc tttggccttg tgacgggtgc agatggcaag aagataaaga cgcgctcggg tgagacggtg aagctgaagg acctcatgga cgaggcggtg gagcggtccc ttgcgatcct gaaggagcgt gaggcgggcg agcgcagcca aggccactcg gaggaagaga tgcagaagct ttccaacatt atcggcattg gcgccatcaa gtacttcgat ctcaagcaga cgcgtactgg tgactacgcc ttcagctacg acaagatgct ggacatgtct ggaaacacag ccgtgttcct tctctatcag tacgcccgca tctgctctat caagcgcaag gccggcatag cggatgagga gctgctcgag atcaccgaca tctctctcga gaccccgcag gagaagagcc tcgcgctgtg cgcgctgcgc ttccagacgg tgattctcaa gaccgttgag gatctcttcc cgcaccacct ggccgacttc gcgtacgagc tcatgacaaa cttctccaac tttttccaaa actgccgcgt cttggatgac ccgctgcaga acagccgcct gtgcctagtc gagcttaccc gcatcaccct caagaagaca ttggagctgc tgaacatcga gacggccgag cgcatttag
Details for LeinA.18295.a.B1.GE42531
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMC AAAATVNVEL ALKEIVKATI AKAFPLVPAQ EPLITLGKVS EYQCNNAMGL VKVLSQQQQP IKMSPQMVGE ELKKNLVGND IIDEFEPTPK GFINVSIRKE WVARMVKEVL KQGIRPPVVT KQRVLVDFSA PNIAKEMHVG HLRSTIIGES ISRLLEFCQH DVARINHIGD WGTAFGMLIL YIKRNFPDYT TNPPDISDLT DFYRAAKKCF DEDQEFKEKA RLEVVKLQAL EEDSIQAWKY ICDVSREEFD KVYTRLGATI EERGESFYNP LIPKVLDLLE EAGQIEVSDG AKLIVSKEAR KLDSLDARDM TKLASQHLAL VSRDGPSYHP NLLAAMKKAG ILTGTEGEES VALSKKEVKP WNKFDIRVDL DKLMAQLAPL YKKQLDPLFL EVFEAAGIVK GDSILVPRFS FPLMVVKSDG GYTYDTTDVT ATYHRFVIEK MNRVIYCTDL GQYEHFRMCM QTAKDMGWME HATWDHAGFG LVTGADGKKI KTRSGETVKL KDLMDEAVER SLAILKEREA GERSQGHSEE EMQKLSNIIG IGAIKYFDLK QTRTGDYAFS YDKMLDMSGN TAVFLLYQYA RICSIKRKAG IADEELLEIT DISLETPQEK SLALCALRFQ TVILKTVEDL FPHHLADFAY ELMTNFSNFF QNCRVLDDPL QNSRLCLVEL TRITLKKTLE LLNIETAERI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgcg ccgctgccgc taccgtgaat gtggagctgg cgctgaagga gattgtcaag gcgaccatcg ccaaggcctt cccgctcgtc ccggcccagg agcctctcat cacccttggc aaggtgagcg agtatcagtg caacaatgcc atggggctgg tgaaggtgct ctctcagcag cagcagccaa taaagatgag cccgcagatg gtcggcgagg agctcaaaaa gaacttggtc ggcaatgata tcatcgacga gttcgaaccc acgcccaagg gtttcattaa tgtcagcatc cgcaaggaat gggtggctag gatggtgaag gaggtgctga aacagggcat ccgcccaccc gtcgtgacga agcagcgcgt gctcgtcgac ttttcagcgc cgaacattgc caaggagatg catgtcggac atctgcgctc caccatcatc ggcgagtcga tcagccgcct gctggagttc tgccagcatg acgttgcacg catcaaccac atcggcgact ggggtacggc gtttggcatg ctcattctgt acatcaagcg caacttcccc gactacacca cgaacccgcc ggacatctct gacctcaccg acttctaccg cgcggcgaag aagtgcttcg acgaggacca ggagttcaag gagaaggccc gcctcgaggt ggtgaagctg caggcgctgg aggaggattc tatccaggcg tggaagtaca tctgcgacgt gtcgcgcgag gagttcgaca aggtttacac ccgcctcggc gccaccatcg aggagcgcgg tgagagcttc tacaacccgc ttattccaaa ggtgctcgat ctgctggagg aggcgggaca gatcgaggtg agtgacggcg cgaagctcat cgtcagcaag gaggcgcgca aactggatag cctcgatgcg cgtgacatga caaagctggc gagtcagcac ctcgcgctcg tctcgcgcga tggtccgtcg taccacccga acctgcttgc cgccatgaag aaggctggca tcttgaccgg gactgagggt gaggagtcgg tggcgctgtc gaagaaggag gtgaagccgt ggaacaagtt tgatatccgc gtggacctgg acaagctgat ggcccagctc gcacccctgt acaagaagca gctcgatcct ctcttcctgg aggtgttcga ggctgcgggg attgtcaagg gcgacagcat ccttgtcccc cgtttctcct tcccgctgat ggtggtgaaa agtgatggcg gctacaccta cgacacgaca gatgtcacgg ccacgtacca ccgcttcgtc atcgagaaga tgaaccgcgt catctactgc accgatttgg gccagtacga gcacttccgc atgtgcatgc agacggcaaa ggacatgggc tggatggaac atgccacatg ggaccacgcc ggctttggcc ttgtgacggg tgcagatggc aagaagataa agacgcgctc gggtgagacg gtgaagctga aggacctcat ggacgaggcg gtggagcggt cccttgcgat cctgaaggag cgtgaggcgg gcgagcgcag ccaaggccac tcggaggaag agatgcagaa gctttccaac attatcggca ttggcgccat caagtacttc gatctcaagc agacgcgtac tggtgactac gccttcagct acgacaagat gctggacatg tctggaaaca cagccgtgtt ccttctctat cagtacgccc gcatctgctc tatcaagcgc aaggccggca tagcggatga ggagctgctc gagatcaccg acatctctct cgagaccccg caggagaaga gcctcgcgct gtgcgcgctg cgcttccaga cggtgattct caagaccgtt gaggatctct tcccgcacca cctggccgac ttcgcgtacg agctcatgac aaacttctcc aactttttcc aaaactgccg cgtcttggat gacccgctgc agaacagccg cctgtgccta gtcgagctta cccgcatcac cctcaagaag acattggagc tgctgaacat cgagacggcc gagcgcattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat