LeinA.18222.a

glutaminyl-tRNA synthetase, putative

CENTER ID: LeinA.18222.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.18222.a.B1.GE42530 full length 1 570
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.15.1490
UniProt: A4HWN1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGPAAAEVK EMETKRDLSI LQNGRPVPGC RNTRELLEAH EKVTGGKPYF RFPPEPNGFL HIGHAKSMNL NFGSARAHGG KCYLRYDDTN PESEDQVYID AIMEMVDWMG WKPDWVTFSS DYFDQLHTFA VQLIKDGKAY VDHSTPDELK QQREQREDSP WRNRSVEENL LLFKHMRQGR YAEGEATLRV KADMKSDNPN MRDFIAYRVK YVEHPHIKDK WCIYPSYDFT HCLIDSLEDI DYSLCTLEFE TRRESYFWLL NELNLWRPYV WEFSRLNVTG SLLSKRKINV LVRKGIVRGF DDPRLLTLAG MRRRGYTPAA INRFCELVGI TRSMNVIQIS MLENTLREDL DERCERRLMV IDPIKVVVDN WKGERIFECP NHPRKPELGS RALTFTDTFY VDRSDFHTDD DNSKFYGLAP GPRVVGLKYS GNVVCKSFEV DAKGQPMVIH VDIDFERKDK PKTNISWVSA TACTPVEVRL YNALLKDDRA AIDPEFLKFI DEKSEVVSHG YAEKGVENFK HFESVQAERF GYFVVDPDTT ADHLVMNRVL GLREDKSKAT VAEPVPAKRK
NT Sequence
atggcgggac cggcagctgc agaagtgaaa gaaatggaga cgaagcgcga cctgtcgatc ctgcagaacg gccgtcccgt cccggggtgc cggaacacgc gcgagctgct cgaggcgcac gagaaggtca ccggcgggaa gccgtacttc cgcttccccc cggagccgaa tggcttcctg cacattggcc atgccaagag catgaacctc aacttcggca gcgcacgtgc gcacggtggc aagtgctacc tgcgctacga cgatacgaac cctgagtcgg aggatcaggt ctacattgac gctatcatgg agatggtgga ctggatgggg tggaaaccgg actgggtcac cttctcctcc gactacttcg accagctgca caccttcgcc gtgcagctca tcaaggatgg gaaggcctac gtcgatcaca gcacgccaga cgagctgaag cagcagcggg agcagcgcga ggacagccca tggcgcaacc gcagcgtcga ggagaatctg ctcctcttca agcacatgcg ccagggccgc tacgccgagg gcgaggcgac gcttcgcgtg aaggccgata tgaagagcga caacccgaac atgcgcgact tcattgcgta ccgcgtcaag tacgtcgagc acccgcacat caaggacaag tggtgcatct acccaagcta cgacttcacc cactgcctca ttgactcgct cgaggacatc gactacagcc tgtgcacgct ggagttcgag acgcgccgcg agagctactt ctggctgctg aacgagctca acctgtggcg tccgtacgtg tgggagttca gccgcctcaa cgtgacgggc tcgctgctct cgaaacgcaa gatcaacgtg ctggtgcgca agggcattgt ccgcgggttt gacgaccccc gcctgctcac ccttgctggc atgcgtcgcc gcgggtacac tccggccgcc attaaccgct tctgcgagct ggttggtatc acccgctcca tgaatgtaat tcagatcagc atgctggaga acaccctgcg cgaggacctc gacgagcgct gcgagcgccg gctgatggtg attgacccaa tcaaggtggt ggtggacaac tggaaggggg agcggatctt cgagtgcccg aaccacccgc gcaagcctga gctcggtagc cgcgccttga catttacgga caccttctac gtcgaccgca gtgacttcca cacagacgac gacaacagca agttctacgg actcgctcct ggcccacgcg tggtgggcct caagtactca ggcaacgttg tgtgcaagag cttcgaggtg gacgccaagg gacagccgat ggtgattcat gtagacatcg acttcgagcg taaagacaag ccgaagacaa atatctcctg ggtgagcgcg acggcatgta cgccggtgga ggtgcgcttg tacaacgcgc ttctcaagga cgaccgtgcc gccatcgatc ccgagtttct caagttcatc gacgagaaga gcgaggtggt gtcgcacggg tacgcggaga agggcgtcga gaacttcaag cacttcgagt cggtccaggc ggaacgcttc gggtactttg tcgtcgaccc tgatacgacg gccgaccacc tcgtcatgaa ccgagtgctg ggcctgcgcg aggacaaaag caaggccacc gttgccgagc cggtgcctgc gaagaggaag tag
Details for LeinA.18222.a.B1.GE42530
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA GPAAAEVKEM ETKRDLSILQ NGRPVPGCRN TCELLEAHEK VTGGKPYFRF PPEPNGFLHI GHAKSMNLNF GSARAHGGKC YLRYDDTNPE SEDQVYIDAI MEMVDWMGWK PDWVTFSSDY FDQLHTFAVQ LIKDGKAYVD HSTPDELKQQ REQREDSPWR NRSVEENLLL FKHMRQGRYA EGEATLRVKA DMKSDNPNMR DFIAYRVXYV EHPHIKDKWC IYPSYDFTHC LIDSLEDIDY SLCTLXXXTR RESYFXLLNE LNLWRXXXXX XSRLNVTGSL LSXXKINVLV RKGIVRGFDD PRLLTLAGMR RRGYTPAAIN RFCELVGITR SMNVIQISML ENTLREDLDE RCERRLMVID PIKVVVDNWK GERIFECPNH PRKPELGSRA LTFTDTFYVD RSDFHTDDDN NKFYGLAPGP RVVGLKYSGN VVCKSFEVDA KGQPMVIHVD IDFERKDKPK TNISWVSATA CTPVEVRLYN ALLKDDRAAI DPEFLKFIDE KSEVVSHGYA EKGVENFKHF ESVQAERFGY FVVDPDTTAD HLVMNRVLGL REDKSKATVA
Validated NT Sequence
atggctcacc accaccacca ccatatggcg ggaccggcag ctgcagaagt gaaagaaatg gagacgaagc gcgacctgtc gatcctgcag aacggccgtc ccgtcccggg gtgccggaac acgtgcgagc tgctcgaggc gcacgagaag gtcaccggcg ggaagccgta cttccgcttc cccccggagc cgaatggctt cctgcacatt ggccatgcca agagcatgaa cctcaacttc ggcagcgcac gtgcgcacgg tggcaagtgc tacctgcgct acgacgatac gaaccctgag tcggaggatc aggtctacat tgacgctatc atggagatgg tggactggat ggggtggaaa ccggactggg tcaccttctc ctccgactac ttcgaccagc tgcacacctt cgccgtgcag ctcatcaagg atgggaaggc ctacgtcgat cacagcacgc cagacgagct gaagcagcag cgggagcagc gcgaggacag cccatggcgc aaccgcagcg tcgaggagaa tctgctcctc ttcaagcaca tgcgccaggg ccgctacgcc gagggcgagg cgacgcttcg cgtgaaggcc gatatgaaga gcgacaaccc gaacatgcgc gacttcattg cgtaccgcgt cnngtacgtc gagcacccgc acatcaagga caagtggtgc atctacccaa gctacgactt cacccactgc ctcattgact cgctcgagga catcgactac agcctgtgca cgctggnnnn nnagacgcgc cgcgagagct acttcnngct gctgaacgag ctcaacctgt ggcgnnnntn cntnnnnnnn nncagccgcc tcaacgtgac gggctcgctg ctctcgaann ntaagatcaa cgtgctggtg cgcaagggca ttgtccgcgg gtttgacgac ccccgcctgc tcacccttgc tggcatgcgt cgccgcgggt acactccggc cgccattaac cgcttctgcg agctggttgg tatcacccgc tccatgaatg taattcagat cagcatgctg gagaacaccc tgcgcgagga cctcgacgag cgctgcgagc gccggctgat ggtgattgac ccaatcaagg tggtggtgga caactggaag ggggagcgga tcttcgagtg cccgaaccac ccgcgcaagc ctgagctcgg tagccgcgcc ttgacattta cggacacctt ctacgtcgac cgcagtgact tccacacaga cgacgacaac aacaagttct acggactcgc ccctggccca cgcgtggtgg gcctcaagta ctcaggcaac gttgtgtgca agagcttcga ggtggacgcc aagggacagc cgatggtgat tcatgtagac atcgacttcg agcgtaaaga caagccgaag acaaatatct cctgggtgag cgcgacggca tgtacgccgg tggaggtgcg cttgtacaac gcgcttctca aggacgaccg tgccgccatc gatcccgagt ttctcaagtt catcgacgag aagagcgagg tggtgtcgca cgggtacgcg gagaagggcg tcgagaactt caagcacttc gagtcggtcc aggcggaacg cttcgggtac tttgtcgtcg accctgatac gacggccgac cacctcgtca tgaaccgagt gctgggcctg cgcgaggaca aaagcaaggc caccgttgcc g
Expected Protein Sequence
MAHHHHHHMA GPAAAEVKEM ETKRDLSILQ NGRPVPGCRN TRELLEAHEK VTGGKPYFRF PPEPNGFLHI GHAKSMNLNF GSARAHGGKC YLRYDDTNPE SEDQVYIDAI MEMVDWMGWK PDWVTFSSDY FDQLHTFAVQ LIKDGKAYVD HSTPDELKQQ REQREDSPWR NRSVEENLLL FKHMRQGRYA EGEATLRVKA DMKSDNPNMR DFIAYRVKYV EHPHIKDKWC IYPSYDFTHC LIDSLEDIDY SLCTLEFETR RESYFWLLNE LNLWRPYVWE FSRLNVTGSL LSKRKINVLV RKGIVRGFDD PRLLTLAGMR RRGYTPAAIN RFCELVGITR SMNVIQISML ENTLREDLDE RCERRLMVID PIKVVVDNWK GERIFECPNH PRKPELGSRA LTFTDTFYVD RSDFHTDDDN SKFYGLAPGP RVVGLKYSGN VVCKSFEVDA KGQPMVIHVD IDFERKDKPK TNISWVSATA CTPVEVRLYN ALLKDDRAAI DPEFLKFIDE KSEVVSHGYA EKGVENFKHF ESVQAERFGY FVVDPDTTAD HLVMNRVLGL REDKSKATVA EPVPAKRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gaccggcagc tgcagaagtg aaagaaatgg agacgaagcg cgacctgtcg atcctgcaga acggccgtcc cgtcccgggg tgccggaaca cgcgcgagct gctcgaggcg cacgagaagg tcaccggcgg gaagccgtac ttccgcttcc ccccggagcc gaatggcttc ctgcacattg gccatgccaa gagcatgaac ctcaacttcg gcagcgcacg tgcgcacggt ggcaagtgct acctgcgcta cgacgatacg aaccctgagt cggaggatca ggtctacatt gacgctatca tggagatggt ggactggatg gggtggaaac cggactgggt caccttctcc tccgactact tcgaccagct gcacaccttc gccgtgcagc tcatcaagga tgggaaggcc tacgtcgatc acagcacgcc agacgagctg aagcagcagc gggagcagcg cgaggacagc ccatggcgca accgcagcgt cgaggagaat ctgctcctct tcaagcacat gcgccagggc cgctacgccg agggcgaggc gacgcttcgc gtgaaggccg atatgaagag cgacaacccg aacatgcgcg acttcattgc gtaccgcgtc aagtacgtcg agcacccgca catcaaggac aagtggtgca tctacccaag ctacgacttc acccactgcc tcattgactc gctcgaggac atcgactaca gcctgtgcac gctggagttc gagacgcgcc gcgagagcta cttctggctg ctgaacgagc tcaacctgtg gcgtccgtac gtgtgggagt tcagccgcct caacgtgacg ggctcgctgc tctcgaaacg caagatcaac gtgctggtgc gcaagggcat tgtccgcggg tttgacgacc cccgcctgct cacccttgct ggcatgcgtc gccgcgggta cactccggcc gccattaacc gcttctgcga gctggttggt atcacccgct ccatgaatgt aattcagatc agcatgctgg agaacaccct gcgcgaggac ctcgacgagc gctgcgagcg ccggctgatg gtgattgacc caatcaaggt ggtggtggac aactggaagg gggagcggat cttcgagtgc ccgaaccacc cgcgcaagcc tgagctcggt agccgcgcct tgacatttac ggacaccttc tacgtcgacc gcagtgactt ccacacagac gacgacaaca gcaagttcta cggactcgct cctggcccac gcgtggtggg cctcaagtac tcaggcaacg ttgtgtgcaa gagcttcgag gtggacgcca agggacagcc gatggtgatt catgtagaca tcgacttcga gcgtaaagac aagccgaaga caaatatctc ctgggtgagc gcgacggcat gtacgccggt ggaggtgcgc ttgtacaacg cgcttctcaa ggacgaccgt gccgccatcg atcccgagtt tctcaagttc atcgacgaga agagcgaggt ggtgtcgcac gggtacgcgg agaagggcgt cgagaacttc aagcacttcg agtcggtcca ggcggaacgc ttcgggtact ttgtcgtcga ccctgatacg acggccgacc acctcgtcat gaaccgagtg ctgggcctgc gcgaggacaa aagcaaggcc accgttgccg agccggtgcc tgcgaagagg aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat