LeinA.10252.a

threonyl-tRNA synthetase, putative

CENTER ID: LeinA.10252.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.10252.a.B1.GE42529 full length 1 788
LeinA.10252.a.B2.GE43054 XtalPred domain #1 22 783
LeinA.10252.a.B3.GE43055 XtalPred domain #2 49 783
LeinA.10252.a.B4.GE43056 XtalPred domain #3 67 783
LeinA.10252.a.B5.GE43800 TEdwards Stma structure domain 285 788
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.10252.a.B2.PW38671 XtalPred domain #1 22 783
LeinA.10252.a.B5.PW38886 TEdwards Stma structure domain 285 788
Molecular models
METHOD RESULTS
comparative modelling Robetta_88905
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.35.1420
UniProt: A4IB34
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKKKAAEA TVDLATLAEP GFWRQRVEIF EQLWQQQQNR YESMKAPIKV SLPDGKVMDA ESWVTCPLDI AKRLSNSLPD KVIVARVNEA LWDLTRPFEA DCQLELLDWD EKDAREVFWH SSSHVLGYAL ERIFDTKLSV GPALEEGGFF YEGLTNRPVS ESDYKAIESA MQELVKQKMP YQRLEVSKED ALRLFGYTEF KSKILASKVP DGGSCTVYRC GMLIDPCRGP HLPDTGRVKA FAVTKNSSSY LEGKAENEVL QRVYGISFPK NPMLTEWKTI QEEAAKRDHR VIGRQQNLFN FHEVSPGSAF WLPHGARIYN TLVEFLRKKY RRCGFQEVIS PNMYSSKLWM VSGHWEKYAD NMFCTKCEKE DFGLKPMNCP GHCIMFASQP HSYKELPIRY ADFGVLHRNE LSGALTGLTR VRRFQQDDAH IFCRMDQITD EMEGQMQFLS DVYGVLGFKF YFYHSTRPAN KLGSEALWDQ SESYLRTALN RFCGIPEELP DPLHPDQRFH YDGKPDSVKK MKALMKKAER CEDPNAWKGP TNGGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPSRFGL KYTLPAAEKD ETTVSPTEKQ KHADPALTAS SPAVEDCVSK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQQVRDT MHDAGFHADV DNGAATLDKK IRNAEKARYN FILVVGQKEQ DATAVNIRAR GEKRLGNKSL VEAVQWLKEL ADTHNREY
NT Sequence
atgtctggca agaagaaggc ggcggaggcg accgtggacc tggccaccct ggcggagcca ggcttctgga ggcaacgcgt cgaaatcttc gagcagctat ggcagcagca gcagaaccgc tatgagtcga tgaaggcccc gatcaaggtg agcctcccgg atggaaaggt gatggacgcc gagagctggg ttacgtgccc gctggacatc gcaaagcgcc tgtccaactc gctgccagac aaggtcattg tggcccgcgt gaacgaggcg ctgtgggacc tgacgcggcc gttcgaggct gactgtcaac tggagctgct ggactgggac gaaaaggatg cgcgtgaggt gttctggcac agctcctcgc acgtgctcgg ctacgcgctc gagcgcatct tcgacacgaa gctctccgtc ggcccggcgc tggaggaggg cggtttcttt tacgagggtc tcaccaatcg cccggtgtcg gagtccgact acaaggcgat cgagtcggcc atgcaggagc tggtgaagca gaagatgcca taccagcgtc tcgaagtgtc gaaggaggac gccctgagac tcttcggcta cacggagttc aagagcaaga tcctggcaag caaggtgccg gacggaggct cgtgcaccgt gtaccggtgc ggtatgctga ttgacccgtg ccgtggtccc cacctcccgg acacgggccg cgtcaaggcc tttgctgtga ccaagaactc ctcctcttac cttgagggca aggccgagaa cgaggtgctg cagcgcgtgt acggcatttc gtttccgaag aaccccatgc tgacggagtg gaagacgatc caggaggagg ccgcgaagcg cgaccaccgc gtaattggcc gccagcagaa cctcttcaac ttccacgagg tctcacctgg cagcgccttc tggctgccgc acggcgcccg gatctacaac actctcgtag aattcctgcg caagaagtat cgccgctgcg gctttcagga ggtcatctcg ccgaacatgt actcctccaa actttggatg gtgtcgggcc actgggagaa gtacgccgat aacatgttct gcaccaagtg cgagaaggag gactttggtc tgaagccgat gaactgcccg ggccactgca tcatgttcgc gtcgcagccg cactcgtaca aggagctgcc gattcgctac gccgactttg gcgtgctgca ccggaacgag ctgagcggtg ccttgacggg gctgacccgc gtgcgccgct tccagcagga cgatgcacac atcttctgcc gcatggacca gatcacagat gagatggagg gccagatgca gttcctcagc gacgtttacg gcgtgctggg cttcaagttc tacttttacc actccacccg ccccgcgaac aagctcggct ctgaagcgct ctgggaccag tccgaatcgt atctgcgcac cgcgctcaac cggttctgcg gcattccgga ggagctgccc gatccgttgc acccggacca gcgctttcac tacgacggca agccggactc ggtcaagaag atgaaggcgc tcatgaagaa ggcggagcgc tgcgaggacc cgaacgcgtg gaagggcccg accaacggcg gcgatggctg gatcgagaac gctggggacg gcgccttcta cggccccaag atcgacatcg tcgtcgagga cgcgctgcgc cgccgccacc agtgcgccac gatccagttg gacttcaacc tgccgagccg cttcggcctc aagtacaccc ttcccgccgc agagaaggac gagacgacgg tgagcccaac ggagaagcag aagcatgccg accctgccct gaccgcgtcg tcgccggccg tggaggattg cgtgagcaaa ccgaagcccg gcacgtacga ggctgctgtg cgtgaccttg gcatcgacct cgagctggac gccaaccagg cccgccccgt catgatccac cgcgccattc tcggttcctt ggagcgctcg atcgccattc tgtgcgagca ctttggcggc gactggccct tctggctgag cccccgccaa gtgatggtgg tgcctgtttc ggcctccaac tacgagtatt cacagcaggt gcgcgacacg atgcacgatg ccggcttcca cgcggacgtc gacaacggtg ccgccacgct ggacaagaag atccgcaatg cggaaaaggc gcgctacaac tttatccttg tcgtgggcca gaaggagcag gacgcgaccg ccgtcaacat ccgcgcgcgc ggggagaaga gactgggcaa caagtcactc gtggaggcgg tgcagtggct caaggagctg gcggacacac ataaccggga gtactag
Details for LeinA.10252.a.B1.GE42529
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 91kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS GKKKAAEATV DLATLAEPGF WRQRVEIFEQ LWQQQQNRYE SMKAPIKVSL PDGKVMDAES WVTCPLDIAK RLSNSLPDKV IVARVNEALW DLTRPFEADC QLELLDWDEK DAREVFWHSS SHVLGYALER IFDTKLSVGP ALEEGGFFYE GLTNRPVSES DYKAIESAMQ ELVKQKMPYQ RLEVSKEDAL RLFGYTEFKS KILASKVPDG GSCTVYRCGM LIDPCRGPHL PDTGRVKAFA VTKNSSSYLE GKAENEVLQR VYGISFPKNP MLTEWKTIQE EAAKRDHRVI GRQQNLFNFH EVSPGSAFWL PHGARIYNTL VEFLRKKYRR CGFQEVISPN MYSSKLWMVS GHWEKYADNM FCTKCEKEDF GLKPMNCPGH CIMFASQPHS YKELPIRYAD FGVLHRNELS GALTGLTRVR RFQQDDAHIF CRMDQITDEM EGQMQFLSDV YGVLGFKFYF YHSTRPANKL GSEALWDQSE SYLRTALNRF CGIPEELPDP LHPDQRFHYD GKPDSVKKMK ALMKKAERCE DPNAWKGPTN GGDGWIENAG DGAFYGPKID IVVEDALRRR HQCATIQLDF NLPSRFGLKY TLPAAEKDET TVSPTEKQKH ADPALTASSP AVEDCVSKPK PGTYEAAVRD LGIDLELDAN QARPVMIHRA ILGSLERSIA ILCEHFGGDW PFWLSPRQVM VVPVSASNYE YSQQVRDTMH DAGFHADVDN GAATLDKKIR NAEKARYNFI LVVGQKEQDA TAVNIRARGE KRLGNKSLVE AVQWLKELAD THNREY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg gcaagaagaa ggcggcggag gcgaccgtgg acctggccac cctggcggag ccaggcttct ggaggcaacg cgtcgaaatc ttcgagcagc tatggcagca gcagcagaac cgctatgagt cgatgaaggc cccgatcaag gtgagcctcc cggatggaaa ggtgatggac gccgagagct gggttacgtg cccgctggac atcgcaaagc gcctgtccaa ctcgctgcca gacaaggtca ttgtggcccg cgtgaacgag gcgctgtggg acctgacgcg gccgttcgag gctgactgtc aactggagct gctggactgg gacgaaaagg atgcgcgtga ggtgttctgg cacagctcct cgcacgtgct cggctacgcg ctcgagcgca tcttcgacac gaagctctcc gtcggcccgg cgctggagga gggcggtttc ttttacgagg gtctcaccaa tcgcccggtg tcggagtccg actacaaggc gatcgagtcg gccatgcagg agctggtgaa gcagaagatg ccataccagc gtctcgaagt gtcgaaggag gacgccctga gactcttcgg ctacacggag ttcaagagca agatcctggc aagcaaggtg ccggacggag gctcgtgcac cgtgtaccgg tgcggtatgc tgattgaccc gtgccgtggt ccccacctcc cggacacggg ccgcgtcaag gcctttgctg tgaccaagaa ctcctcctct taccttgagg gcaaggccga gaacgaggtg ctgcagcgcg tgtacggcat ttcgtttccg aagaacccca tgctgacgga gtggaagacg atccaggagg aggccgcgaa gcgcgaccac cgcgtaattg gccgccagca gaacctcttc aacttccacg aggtctcacc tggcagcgcc ttctggctgc cgcacggcgc ccggatctac aacactctcg tagaattcct gcgcaagaag tatcgccgct gcggctttca ggaggtcatc tcgccgaaca tgtactcctc caaactttgg atggtgtcgg gccactggga gaagtacgcc gataacatgt tctgcaccaa gtgcgagaag gaggactttg gtctgaagcc gatgaactgc ccgggccact gcatcatgtt cgcgtcgcag ccgcactcgt acaaggagct gccgattcgc tacgccgact ttggcgtgct gcaccggaac gagctgagcg gtgccttgac ggggctgacc cgcgtgcgcc gcttccagca ggacgatgca cacatcttct gccgcatgga ccagatcaca gatgagatgg agggccagat gcagttcctc agcgacgttt acggcgtgct gggcttcaag ttctactttt accactccac ccgccccgcg aacaagctcg gctctgaagc gctctgggac cagtccgaat cgtatctgcg caccgcgctc aaccggttct gcggcattcc ggaggagctg cccgatccgt tgcacccgga ccagcgcttt cactacgacg gcaagccgga ctcggtcaag aagatgaagg cgctcatgaa gaaggcggag cgctgcgagg acccgaacgc gtggaagggc ccgaccaacg gcggcgatgg ctggatcgag aacgctgggg acggcgcctt ctacggcccc aagatcgaca tcgtcgtcga ggacgcgctg cgccgccgcc accagtgcgc cacgatccag ttggacttca acctgccgag ccgcttcggc ctcaagtaca cccttcccgc cgcagagaag gacgagacga cggtgagccc aacggagaag cagaagcatg ccgaccctgc cctgaccgcg tcgtcgccgg ccgtggagga ttgcgtgagc aaaccgaagc ccggcacgta cgaggctgct gtgcgtgacc ttggcatcga cctcgagctg gacgccaacc aggcccgccc cgtcatgatc caccgcgcca ttctcggttc cttggagcgc tcgatcgcca ttctgtgcga gcactttggc ggcgactggc ccttctggct gagcccccgc caagtgatgg tggtgcctgt ttcggcctcc aactacgagt attcacagca ggtgcgcgac acgatgcacg atgccggctt ccacgcggac gtcgacaacg gtgccgccac gctggacaag aagatccgca atgcggaaaa ggcgcgctac aactttatcc ttgtcgtggg ccagaaggag caggacgcga ccgccgtcaa catccgcgcg cgcggggaga agagactggg caacaagtca ctcgtggagg cggtgcagtg gctcaaggag ctggcggaca cacataaccg ggagtactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LeinA.10252.a.B2.GE43054
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 88kDa
OBSERVED MW: 88kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHFW RQRVEIFEQL WQQQQNRYES MKAPIKVSLP DGKVMDAESW VTCPLDIAKR LSNSLPDKVI VARVNEALWD LTRPFEADCQ LELLDWDEKD AREVFWHSSS HVLGYALERI FDTKLSVGPA LEEGGFFYEG LTNRPVSESD YKAIESAMQE LVKQKMPYQR LEVSKEDALR LFGYTEFKSK ILASKVPDGG SCTVYRCGML IDPCRGPHLP DTGRVKAFAV TKNSSSYLEG KAENEVLQRV YGISFPKNPM LTEWKTIQEE AAKRDHRVIG RQQNLFNFHE VSPGSAFWLP HGARIYNTLV EFLRKKYRRC GFQEVISPNM YSSKLWMVSG HWEKYADNMF CTKCEKEDFG LKPMNCPGHC IMFASQPHSY KELPIRYADF GVLHRNELSG ALTGLTRVRR FQQDDAHIFC RMDQITDEME GQMQFLSDVY GVLGFKFYFY HSTRPANKLG SEALWDQSES YLRTALNRFC GIPEELPDPL HPDQRFHYDG KPDSVKKMKA LMKKAERCED PNAWKGPTNG GDGWIENAGD GAFYGPKIDI VVEDALRRRH QCATIQLDFN LPSRFGLKYT LPAAEKDETT VSPTEKQKHA DPALTASSPA VEDCVSKPKP GTYEAAVRDL GIDLELDANQ ARPVMIHRAI LGSLERSIAI LCEHFGGDWP FWLSPRQVMV VPVSASNYEY SQQVRDTMHD AGFHADVDNG AATLDKKIRN AEKARYNFIL VVGQKEQDAT AVNIRARGEK RLGNKSLVEA VQWLKELADT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttctgga ggcaacgcgt cgaaatcttc gagcagctat ggcagcagca gcagaaccgc tatgagtcga tgaaggcccc gatcaaggtg agcctcccgg atggaaaggt gatggacgcc gagagctggg ttacgtgccc gctggacatc gcaaagcgcc tgtccaactc gctgccagac aaggtcattg tggcccgcgt gaacgaggcg ctgtgggacc tgacgcggcc gttcgaggct gactgtcaac tggagctgct ggactgggac gaaaaggatg cgcgtgaggt gttctggcac agctcctcgc acgtgctcgg ctacgcgctc gagcgcatct tcgacacgaa gctctccgtc ggcccggcgc tggaggaggg cggtttcttt tacgagggtc tcaccaatcg cccggtgtcg gagtccgact acaaggcgat cgagtcggcc atgcaggagc tggtgaagca gaagatgcca taccagcgtc tcgaagtgtc gaaggaggac gccctgagac tcttcggcta cacggagttc aagagcaaga tcctggcaag caaggtgccg gacggaggct cgtgcaccgt gtaccggtgc ggtatgctga ttgacccgtg ccgtggtccc cacctcccgg acacgggccg cgtcaaggcc tttgctgtga ccaagaactc ctcctcttac cttgagggca aggccgagaa cgaggtgctg cagcgcgtgt acggcatttc gtttccgaag aaccccatgc tgacggagtg gaagacgatc caggaggagg ccgcgaagcg cgaccaccgc gtaattggcc gccagcagaa cctcttcaac ttccacgagg tctcacctgg cagcgccttc tggctgccgc acggcgcccg gatctacaac actctcgtag aattcctgcg caagaagtat cgccgctgcg gctttcagga ggtcatctcg ccgaacatgt actcctccaa actttggatg gtgtcgggcc actgggagaa gtacgccgat aacatgttct gcaccaagtg cgagaaggag gactttggtc tgaagccgat gaactgcccg ggccactgca tcatgttcgc gtcgcagccg cactcgtaca aggagctgcc gattcgctac gccgactttg gcgtgctgca ccggaacgag ctgagcggtg ccttgacggg gctgacccgc gtgcgccgct tccagcagga cgatgcacac atcttctgcc gcatggacca gatcacagat gagatggagg gccagatgca gttcctcagc gacgtttacg gcgtgctggg cttcaagttc tacttttacc actccacccg ccccgcgaac aagctcggct ctgaagcgct ctgggaccag tccgaatcgt atctgcgcac cgcgctcaac cggttctgcg gcattccgga ggagctgccc gatccgttgc acccggacca gcgctttcac tacgacggca agccggactc ggtcaagaag atgaaggcgc tcatgaagaa ggcggagcgc tgcgaggacc cgaacgcgtg gaagggcccg accaacggcg gcgatggctg gatcgagaac gctggggacg gcgccttcta cggccccaag atcgacatcg tcgtcgagga cgcgctgcgc cgccgccacc agtgcgccac gatccagttg gacttcaacc tgccgagccg cttcggcctc aagtacaccc ttcccgccgc agagaaggac gagacgacgg tgagcccaac ggagaagcag aagcatgccg accctgccct gaccgcgtcg tcgccggccg tggaggattg cgtgagcaaa ccgaagcccg gcacgtacga ggctgctgtg cgtgaccttg gcatcgacct cgagctggac gccaaccagg cccgccccgt catgatccac cgcgccattc tcggttcctt ggagcgctcg atcgccattc tgtgcgagca ctttggcggc gactggccct tctggctgag cccccgccaa gtgatggtgg tgcctgtttc ggcctccaac tacgagtatt cacagcaggt gcgcgacacg atgcacgatg ccggcttcca cgcggacgtc gacaacggtg ccgccacgct ggacaagaag atccgcaatg cggaaaaggc gcgctacaac tttatccttg tcgtgggcca gaaggagcag gacgcgaccg ccgtcaacat ccgcgcgcgc ggggagaaga gactgggcaa caagtcactc gtggaggcgg tgcagtggct caaggagctg gcggacacat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LeinA.10252.a.B3.GE43055
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 84kDa
OBSERVED MW: 84kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHKV SLPDGKVMDA ESWVTCPLDI AKRLSNSLPD KVIVARVNEA LWDLTRPFEA DCQLELLDWD EKDAREVFWH SSSHVLGYAL ERIFDTKLSV GPALEEGGFF YEGLTNRPVS ESDYKAIESA MQELVKQKMP YQRLEVSKED ALRLFGYTEF KSKILASKVP DGGSCTVYRC GMLIDPCRGP HLPDTGRVKA FAVTKNSSSY LEGKAENEVL QRVYGISFPK NPMLTEWKTI QEEAAKRDHR VIGRQQNLFN FHEVSPGSAF WLPHGARIYN TLVEFLRKKY RRCGFQEVIS PNMYSSKLWM VSGHWEKYAD NMFCTKCEKE DFGLKPMNCP GHCIMFASQP HSYKELPIRY ADFGVLHRNE LSGALTGLTR VRRFQQDDAH IFCRMDQITD EMEGQMQFLS DVYGVLGFKF YFYHSTRPAN KLGSEALWDQ SESYLRTALN RFCGIPEELP DPLHPDQRFH YDGKPDSVKK MKALMKKAER CEDPNAWKGP TNGGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPSRFGL KYTLPAAEKD ETTVSPTEKQ KHADPALTAS SPAVEDCVSK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQQVRDT MHDAGFHADV DNGAATLDKK IRNAEKARYN FILVVGQKEQ DATAVNIRAR GEKRLGNKSL VEAVQWLKEL ADT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaggtga gcctcccgga tggaaaggtg atggacgccg agagctgggt tacgtgcccg ctggacatcg caaagcgcct gtccaactcg ctgccagaca aggtcattgt ggcccgcgtg aacgaggcgc tgtgggacct gacgcggccg ttcgaggctg actgtcaact ggagctgctg gactgggacg aaaaggatgc gcgtgaggtg ttctggcaca gctcctcgca cgtgctcggc tacgcgctcg agcgcatctt cgacacgaag ctctccgtcg gcccggcgct ggaggagggc ggtttctttt acgagggtct caccaatcgc ccggtgtcgg agtccgacta caaggcgatc gagtcggcca tgcaggagct ggtgaagcag aagatgccat accagcgtct cgaagtgtcg aaggaggacg ccctgagact cttcggctac acggagttca agagcaagat cctggcaagc aaggtgccgg acggaggctc gtgcaccgtg taccggtgcg gtatgctgat tgacccgtgc cgtggtcccc acctcccgga cacgggccgc gtcaaggcct ttgctgtgac caagaactcc tcctcttacc ttgagggcaa ggccgagaac gaggtgctgc agcgcgtgta cggcatttcg tttccgaaga accccatgct gacggagtgg aagacgatcc aggaggaggc cgcgaagcgc gaccaccgcg taattggccg ccagcagaac ctcttcaact tccacgaggt ctcacctggc agcgccttct ggctgccgca cggcgcccgg atctacaaca ctctcgtaga attcctgcgc aagaagtatc gccgctgcgg ctttcaggag gtcatctcgc cgaacatgta ctcctccaaa ctttggatgg tgtcgggcca ctgggagaag tacgccgata acatgttctg caccaagtgc gagaaggagg actttggtct gaagccgatg aactgcccgg gccactgcat catgttcgcg tcgcagccgc actcgtacaa ggagctgccg attcgctacg ccgactttgg cgtgctgcac cggaacgagc tgagcggtgc cttgacgggg ctgacccgcg tgcgccgctt ccagcaggac gatgcacaca tcttctgccg catggaccag atcacagatg agatggaggg ccagatgcag ttcctcagcg acgtttacgg cgtgctgggc ttcaagttct acttttacca ctccacccgc cccgcgaaca agctcggctc tgaagcgctc tgggaccagt ccgaatcgta tctgcgcacc gcgctcaacc ggttctgcgg cattccggag gagctgcccg atccgttgca cccggaccag cgctttcact acgacggcaa gccggactcg gtcaagaaga tgaaggcgct catgaagaag gcggagcgct gcgaggaccc gaacgcgtgg aagggcccga ccaacggcgg cgatggctgg atcgagaacg ctggggacgg cgccttctac ggccccaaga tcgacatcgt cgtcgaggac gcgctgcgcc gccgccacca gtgcgccacg atccagttgg acttcaacct gccgagccgc ttcggcctca agtacaccct tcccgccgca gagaaggacg agacgacggt gagcccaacg gagaagcaga agcatgccga ccctgccctg accgcgtcgt cgccggccgt ggaggattgc gtgagcaaac cgaagcccgg cacgtacgag gctgctgtgc gtgaccttgg catcgacctc gagctggacg ccaaccaggc ccgccccgtc atgatccacc gcgccattct cggttccttg gagcgctcga tcgccattct gtgcgagcac tttggcggcg actggccctt ctggctgagc ccccgccaag tgatggtggt gcctgtttcg gcctccaact acgagtattc acagcaggtg cgcgacacga tgcacgatgc cggcttccac gcggacgtcg acaacggtgc cgccacgctg gacaagaaga tccgcaatgc ggaaaaggcg cgctacaact ttatccttgt cgtgggccag aaggagcagg acgcgaccgc cgtcaacatc cgcgcgcgcg gggagaagag actgggcaac aagtcactcg tggaggcggt gcagtggctc aaggagctgg cggacacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LeinA.10252.a.B4.GE43056
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 82kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHPL DIAKRLSNSL PDKVIVARVN EALWDLTRPF EADCQLELLD WDEKDAREVF WHSSSHVLGY ALERIFDTKL SVGPALEEGG FFYEGLTNRP VSESDYKAIE SAMQELVKQK MPYQRLEVSK EDALRLFGYT EFKSKILASK VPDGGSCTVY RCGMLIDPCR GPHLPDTGRV KAFAVTKNSS SYLEGKAENE VLQRVYGISF PKNPMLTEWK TIQEEAAKRD HRVIGRQQNL FNFHEVSPGS AFWLPHGARI YNTLVEFLRK KYRRCGFQEV ISPNMYSSKL WMVSGHWEKY ADNMFCTKCE KEDFGLKPMN CPGHCIMFAS QPHSYKELPI RYADFGVLHR NELSGALTGL TRVRRFQQDD AHIFCRMDQI TDEMEGQMQF LSDVYGVLGF KFYFYHSTRP ANKLGSEALW DQSESYLRTA LNRFCGIPEE LPDPLHPDQR FHYDGKPDSV KKMKALMKKA ERCEDPNAWK GPTNGGDGWI ENAGDGAFYG PKIDIVVEDA LRRRHQCATI QLDFNLPSRF GLKYTLPAAE KDETTVSPTE KQKHADPALT ASSPAVEDCV SKPKPGTYEA AVRDLGIDLE LDANQARPVM IHRAILGSLE RSIAILCEHF GGDWPFWLSP RQVMVVPVSA SNYEYSQQVR DTMHDAGFHA DVDNGAATLD KKIRNAEKAR YNFILVVGQK EQDATAVNIR ARGEKRLGNK SLVEAVQWLK ELADT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccgctgg acatcgcaaa gcgcctgtcc aactcgctgc cagacaaggt cattgtggcc cgcgtgaacg aggcgctgtg ggacctgacg cggccgttcg aggctgactg tcaactggag ctgctggact gggacgaaaa ggatgcgcgt gaggtgttct ggcacagctc ctcgcacgtg ctcggctacg cgctcgagcg catcttcgac acgaagctct ccgtcggccc ggcgctggag gagggcggtt tcttttacga gggtctcacc aatcgcccgg tgtcggagtc cgactacaag gcgatcgagt cggccatgca ggagctggtg aagcagaaga tgccatacca gcgtctcgaa gtgtcgaagg aggacgccct gagactcttc ggctacacgg agttcaagag caagatcctg gcaagcaagg tgccggacgg aggctcgtgc accgtgtacc ggtgcggtat gctgattgac ccgtgccgtg gtccccacct cccggacacg ggccgcgtca aggcctttgc tgtgaccaag aactcctcct cttaccttga gggcaaggcc gagaacgagg tgctgcagcg cgtgtacggc atttcgtttc cgaagaaccc catgctgacg gagtggaaga cgatccagga ggaggccgcg aagcgcgacc accgcgtaat tggccgccag cagaacctct tcaacttcca cgaggtctca cctggcagcg ccttctggct gccgcacggc gcccggatct acaacactct cgtagaattc ctgcgcaaga agtatcgccg ctgcggcttt caggaggtca tctcgccgaa catgtactcc tccaaacttt ggatggtgtc gggccactgg gagaagtacg ccgataacat gttctgcacc aagtgcgaga aggaggactt tggtctgaag ccgatgaact gcccgggcca ctgcatcatg ttcgcgtcgc agccgcactc gtacaaggag ctgccgattc gctacgccga ctttggcgtg ctgcaccgga acgagctgag cggtgccttg acggggctga cccgcgtgcg ccgcttccag caggacgatg cacacatctt ctgccgcatg gaccagatca cagatgagat ggagggccag atgcagttcc tcagcgacgt ttacggcgtg ctgggcttca agttctactt ttaccactcc acccgccccg cgaacaagct cggctctgaa gcgctctggg accagtccga atcgtatctg cgcaccgcgc tcaaccggtt ctgcggcatt ccggaggagc tgcccgatcc gttgcacccg gaccagcgct ttcactacga cggcaagccg gactcggtca agaagatgaa ggcgctcatg aagaaggcgg agcgctgcga ggacccgaac gcgtggaagg gcccgaccaa cggcggcgat ggctggatcg agaacgctgg ggacggcgcc ttctacggcc ccaagatcga catcgtcgtc gaggacgcgc tgcgccgccg ccaccagtgc gccacgatcc agttggactt caacctgccg agccgcttcg gcctcaagta cacccttccc gccgcagaga aggacgagac gacggtgagc ccaacggaga agcagaagca tgccgaccct gccctgaccg cgtcgtcgcc ggccgtggag gattgcgtga gcaaaccgaa gcccggcacg tacgaggctg ctgtgcgtga ccttggcatc gacctcgagc tggacgccaa ccaggcccgc cccgtcatga tccaccgcgc cattctcggt tccttggagc gctcgatcgc cattctgtgc gagcactttg gcggcgactg gcccttctgg ctgagccccc gccaagtgat ggtggtgcct gtttcggcct ccaactacga gtattcacag caggtgcgcg acacgatgca cgatgccggc ttccacgcgg acgtcgacaa cggtgccgcc acgctggaca agaagatccg caatgcggaa aaggcgcgct acaactttat ccttgtcgtg ggccagaagg agcaggacgc gaccgccgtc aacatccgcg cgcgcgggga gaagagactg ggcaacaagt cactcgtgga ggcggtgcag tggctcaagg agctggcgga cacatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LeinA.10252.a.B5.GE43800
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 76
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMA KRDHRVIGRQ QNLFNFHEVS PGSAFWLPHG ARIYNTLVEF LRKKYRRCGF QEVISPNMYS SKLWMVSGHW EKYADNMFCT KCEKEDFGLK PMNCPGHCIM FASQPHSYKE LPIRYADFGV LHRNELSGAL TGLTRVRRFQ QDDAHIFCRM DQITDEMEGQ MQFLSDVYGV LGFKFYFYHS TRPANKLGSD ALWDQSESYL RTALNRFCGI PXELPDPLHP DQRFHYDGKP DSVKKMKALM KXXERCEDPN AWXGPTNGGD GWIENXWGXR LLRPXDRHRR XGRAAXXAHX XXXDPXWXSX XXAXFGXXYT LXPXXXTXRX XXPXXXXXCR XXXXXXXXXP XXXXXXXXPX XXXXX
Validated NT Sequence
cnnnnnnann nactcactat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg gcgaagcgcg accaccgcgt aattggccgc cagcagaacc tcttcaactt ccacgaggtc tcacctggca gcgccttctg gctgccgcac ggcgcccgga tctacaacac tctcgtagaa ttcctgcgca agaagtatcg ccgctgcggc tttcaggagg tcatctcgcc gaacatgtac tcctccaaac tttggatggt gtcgggccac tgggagaagt acgccgataa catgttctgc accaagtgcg agaaggagga ctttggtctg aagccgatga actgcccggg ccactgcatc atgttcgcgt cgcagccgca ctcgtacaag gagctgccga ttcgctacgc cgactttggc gtgctgcacc ggaacgagct gagcggtgcc ttgacggggc tgacccgcgt gcgccgcttc cagcaggacg atgcacacat cttctgccgc atggaccaga tcacagatga gatggagggc cagatgcagt tcctcagcga cgtttacggc gtgctgggct tcaagttcta cttttaccac tccacccgcc ccgcgaacaa gctcggctct gacgcgctct gggaccagtc cgaatcgtat ctgcgcaccg cgctcaaccg gttctgcggc attccggang agctgcccga tccgttgcac ccggaccagc gctttcacta cgacggcaag ccggactcgg tcaagaagat gaaggcgctc atgaagaang nggagcgctg cgaggacccg aacgcgtgga anggcccgac caacggcggc gatggctgga tcgagaacnn ctggggangg cgccttctac ggccccanga tcgacatcgt cgtcnaggac gngctgcnnc cnccgcccac canngnnccn ncgatccagn ttggnnttcn anctnncnag ccnncttcgg ncncangtac accctnnngc cgcngannan gacnanacga ngnnnnancc cnngganann canancatgc cganccnngc nnnncnnnnn nnnnnccgnn ccgngnnnnn gnnnnnnnnn annnannccn nnncnnnnnn nangnntgnc
Expected Protein Sequence
MAHHHHHHAK RDHRVIGRQQ NLFNFHEVSP GSAFWLPHGA RIYNTLVEFL RKKYRRCGFQ EVISPNMYSS KLWMVSGHWE KYADNMFCTK CEKEDFGLKP MNCPGHCIMF ASQPHSYKEL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QITDEMEGQM QFLSDVYGVL GFKFYFYHST RPANKLGSEA LWDQSESYLR TALNRFCGIP EELPDPLHPD QRFHYDGKPD SVKKMKALMK KAERCEDPNA WKGPTNGGDG WIENAGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPS RFGLKYTLPA AEKDETTVSP TEKQKHADPA LTASSPAVED CVSKPKPGTY EAAVRDLGID LELDANQARP VMIHRAILGS LERSIAILCE HFGGDWPFWL SPRQVMVVPV SASNYEYSQQ VRDTMHDAGF HADVDNGAAT LDKKIRNAEK ARYNFILVVG QKEQDATAVN IRARGEKRLG NKSLVEAVQW LKELADTHNR EY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgaagc gcgaccaccg cgtaattggc cgccagcaga acctcttcaa cttccacgag gtctcacctg gcagcgcctt ctggctgccg cacggcgccc ggatctacaa cactctcgta gaattcctgc gcaagaagta tcgccgctgc ggctttcagg aggtcatctc gccgaacatg tactcctcca aactttggat ggtgtcgggc cactgggaga agtacgccga taacatgttc tgcaccaagt gcgagaagga ggactttggt ctgaagccga tgaactgccc gggccactgc atcatgttcg cgtcgcagcc gcactcgtac aaggagctgc cgattcgcta cgccgacttt ggcgtgctgc accggaacga gctgagcggt gccttgacgg ggctgacccg cgtgcgccgc ttccagcagg acgatgcaca catcttctgc cgcatggacc agatcacaga tgagatggag ggccagatgc agttcctcag cgacgtttac ggcgtgctgg gcttcaagtt ctacttttac cactccaccc gccccgcgaa caagctcggc tctgaagcgc tctgggacca gtccgaatcg tatctgcgca ccgcgctcaa ccggttctgc ggcattccgg aggagctgcc cgatccgttg cacccggacc agcgctttca ctacgacggc aagccggact cggtcaagaa gatgaaggcg ctcatgaaga aggcggagcg ctgcgaggac ccgaacgcgt ggaagggccc gaccaacggc ggcgatggct ggatcgagaa cgctggggac ggcgccttct acggccccaa gatcgacatc gtcgtcgagg acgcgctgcg ccgccgccac cagtgcgcca cgatccagtt ggacttcaac ctgccgagcc gcttcggcct caagtacacc cttcccgccg cagagaagga cgagacgacg gtgagcccaa cggagaagca gaagcatgcc gaccctgccc tgaccgcgtc gtcgccggcc gtggaggatt gcgtgagcaa accgaagccc ggcacgtacg aggctgctgt gcgtgacctt ggcatcgacc tcgagctgga cgccaaccag gcccgccccg tcatgatcca ccgcgccatt ctcggttcct tggagcgctc gatcgccatt ctgtgcgagc actttggcgg cgactggccc ttctggctga gcccccgcca agtgatggtg gtgcctgttt cggcctccaa ctacgagtat tcacagcagg tgcgcgacac gatgcacgat gccggcttcc acgcggacgt cgacaacggt gccgccacgc tggacaagaa gatccgcaat gcggaaaagg cgcgctacaa ctttatcctt gtcgtgggcc agaaggagca ggacgcgacc gccgtcaaca tccgcgcgcg cggggagaag agactgggca acaagtcact cgtggaggcg gtgcagtggc tcaaggagct ggcggacaca cataaccggg agtactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LeinA.10252.a.B2.PW38671
PURIFICATION DATe: 7/24/2019
CONCENTRATION: 12.9mg/ml
OBSERVED MW: 88kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHFW RQRVEIFEQL WQQQQNRYES MKAPIKVSLP DGKVMDAESW VTCPLDIAKR LSNSLPDKVI VARVNEALWD LTRPFEADCQ LELLDWDEKD AREVFWHSSS HVLGYALERI FDTKLSVGPA LEEGGFFYEG LTNRPVSESD YKAIESAMQE LVKQKMPYQR LEVSKEDALR LFGYTEFKSK ILASKVPDGG SCTVYRCGML IDPCRGPHLP DTGRVKAFAV TKNSSSYLEG KAENEVLQRV YGISFPKNPM LTEWKTIQEE AAKRDHRVIG RQQNLFNFHE VSPGSAFWLP HGARIYNTLV EFLRKKYRRC GFQEVISPNM YSSKLWMVSG HWEKYADNMF CTKCEKEDFG LKPMNCPGHC IMFASQPHSY KELPIRYADF GVLHRNELSG ALTGLTRVRR FQQDDAHIFC RMDQITDEME GQMQFLSDVY GVLGFKFYFY HSTRPANKLG SEALWDQSES YLRTALNRFC GIPEELPDPL HPDQRFHYDG KPDSVKKMKA LMKKAERCED PNAWKGPTNG GDGWIENAGD GAFYGPKIDI VVEDALRRRH QCATIQLDFN LPSRFGLKYT LPAAEKDETT VSPTEKQKHA DPALTASSPA VEDCVSKPKP GTYEAAVRDL GIDLELDANQ ARPVMIHRAI LGSLERSIAI LCEHFGGDWP FWLSPRQVMV VPVSASNYEY SQQVRDTMHD AGFHADVDNG AATLDKKIRN AEKARYNFIL VVGQKEQDAT AVNIRARGEK RLGNKSLVEA VQWLKELADT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTCTGGA GGCAACGCGT CGAAATCTTC GAGCAGCTAT GGCAGCAGCA GCAGAACCGC TATGAGTCGA TGAAGGCCCC GATCAAGGTG AGCCTCCCGG ATGGAAAGGT GATGGACGCC GAGAGCTGGG TTACGTGCCC GCTGGACATC GCAAAGCGCC TGTCCAACTC GCTGCCAGAC AAGGTCATTG TGGCCCGCGT GAACGAGGCG CTGTGGGACC TGACGCGGCC GTTCGAGGCT GACTGTCAAC TGGAGCTGCT GGACTGGGAC GAAAAGGATG CGCGTGAGGT GTTCTGGCAC AGCTCCTCGC ACGTGCTCGG CTACGCGCTC GAGCGCATCT TCGACACGAA GCTCTCCGTC GGCCCGGCGC TGGAGGAGGG CGGTTTCTTT TACGAGGGTC TCACCAATCG CCCGGTGTCG GAGTCCGACT ACAAGGCGAT CGAGTCGGCC ATGCAGGAGC TGGTGAAGCA GAAGATGCCA TACCAGCGTC TCGAAGTGTC GAAGGAGGAC GCCCTGAGAC TCTTCGGCTA CACGGAGTTC AAGAGCAAGA TCCTGGCAAG CAAGGTGCCG GACGGAGGCT CGTGCACCGT GTACCGGTGC GGTATGCTGA TTGACCCGTG CCGTGGTCCC CACCTCCCGG ACACGGGCCG CGTCAAGGCC TTTGCTGTGA CCAAGAACTC CTCCTCTTAC CTTGAGGGCA AGGCCGAGAA CGAGGTGCTG CAGCGCGTGT ACGGCATTTC GTTTCCGAAG AACCCCATGC TGACGGAGTG GAAGACGATC CAGGAGGAGG CCGCGAAGCG CGACCACCGC GTAATTGGCC GCCAGCAGAA CCTCTTCAAC TTCCACGAGG TCTCACCTGG CAGCGCCTTC TGGCTGCCGC ACGGCGCCCG GATCTACAAC ACTCTCGTAG AATTCCTGCG CAAGAAGTAT CGCCGCTGCG GCTTTCAGGA GGTCATCTCG CCGAACATGT ACTCCTCCAA ACTTTGGATG GTGTCGGGCC ACTGGGAGAA GTACGCCGAT AACATGTTCT GCACCAAGTG CGAGAAGGAG GACTTTGGTC TGAAGCCGAT GAACTGCCCG GGCCACTGCA TCATGTTCGC GTCGCAGCCG CACTCGTACA AGGAGCTGCC GATTCGCTAC GCCGACTTTG GCGTGCTGCA CCGGAACGAG CTGAGCGGTG CCTTGACGGG GCTGACCCGC GTGCGCCGCT TCCAGCAGGA CGATGCACAC ATCTTCTGCC GCATGGACCA GATCACAGAT GAGATGGAGG GCCAGATGCA GTTCCTCAGC GACGTTTACG GCGTGCTGGG CTTCAAGTTC TACTTTTACC ACTCCACCCG CCCCGCGAAC AAGCTCGGCT CTGAAGCGCT CTGGGACCAG TCCGAATCGT ATCTGCGCAC CGCGCTCAAC CGGTTCTGCG GCATTCCGGA GGAGCTGCCC GATCCGTTGC ACCCGGACCA GCGCTTTCAC TACGACGGCA AGCCGGACTC GGTCAAGAAG ATGAAGGCGC TCATGAAGAA GGCGGAGCGC TGCGAGGACC CGAACGCGTG GAAGGGCCCG ACCAACGGCG GCGATGGCTG GATCGAGAAC GCTGGGGACG GCGCCTTCTA CGGCCCCAAG ATCGACATCG TCGTCGAGGA CGCGCTGCGC CGCCGCCACC AGTGCGCCAC GATCCAGTTG GACTTCAACC TGCCGAGCCG CTTCGGCCTC AAGTACACCC TTCCCGCCGC AGAGAAGGAC GAGACGACGG TGAGCCCAAC GGAGAAGCAG AAGCATGCCG ACCCTGCCCT GACCGCGTCG TCGCCGGCCG TGGAGGATTG CGTGAGCAAA CCGAAGCCCG GCACGTACGA GGCTGCTGTG CGTGACCTTG GCATCGACCT CGAGCTGGAC GCCAACCAGG CCCGCCCCGT CATGATCCAC CGCGCCATTC TCGGTTCCTT GGAGCGCTCG ATCGCCATTC TGTGCGAGCA CTTTGGCGGC GACTGGCCCT TCTGGCTGAG CCCCCGCCAA GTGATGGTGG TGCCTGTTTC GGCCTCCAAC TACGAGTATT CACAGCAGGT GCGCGACACG ATGCACGATG CCGGCTTCCA CGCGGACGTC GACAACGGTG CCGCCACGCT GGACAAGAAG ATCCGCAATG CGGAAAAGGC GCGCTACAAC TTTATCCTTG TCGTGGGCCA GAAGGAGCAG GACGCGACCG CCGTCAACAT CCGCGCGCGC GGGGAGAAGA GACTGGGCAA CAAGTCACTC GTGGAGGCGG TGCAGTGGCT CAAGGAGCTG GCGGACACAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for LeinA.10252.a.B5.PW38886
PURIFICATION DATe: 9/2/2020
CONCENTRATION: 24mg/ml
OBSERVED MW: 58kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 76
PERCENT COVERAGE: 71
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KRDHRVIGRQ QNLFNFHEVS PGSAFWLPHG ARIYNTLVEF LRKKYRRCGF QEVISPNMYS SKLWMVSGHW EKYADNMFCT KCEKEDFGLK PMNCPGHCIM FASQPHSYKE LPIRYADFGV LHRNELSGAL TGLTRVRRFQ QDDAHIFCRM DQITDEMEGQ MQFLSDVYGV LGFKFYFYHS TRPANKLGSD ALWDQSESYL RTALNRFCGI PXELPDPLHP DQRFHYDGKP DSVKKMKALM KXXERCEDPN AWXGPTNGGD GWIENXWGXR LLRPXDRHRR XGRAAXXAHX XXXDPXWXSX XXAXFGXXYT LXPXXXTXRX XXPXXXXXCR XXXXXXXXXP XXXXXXXXPX XXXXX
Validated NT Sequence
cnnnnnnann nactcactat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg gcgaagcgcg accaccgcgt aattggccgc cagcagaacc tcttcaactt ccacgaggtc tcacctggca gcgccttctg gctgccgcac ggcgcccgga tctacaacac tctcgtagaa ttcctgcgca agaagtatcg ccgctgcggc tttcaggagg tcatctcgcc gaacatgtac tcctccaaac tttggatggt gtcgggccac tgggagaagt acgccgataa catgttctgc accaagtgcg agaaggagga ctttggtctg aagccgatga actgcccggg ccactgcatc atgttcgcgt cgcagccgca ctcgtacaag gagctgccga ttcgctacgc cgactttggc gtgctgcacc ggaacgagct gagcggtgcc ttgacggggc tgacccgcgt gcgccgcttc cagcaggacg atgcacacat cttctgccgc atggaccaga tcacagatga gatggagggc cagatgcagt tcctcagcga cgtttacggc gtgctgggct tcaagttcta cttttaccac tccacccgcc ccgcgaacaa gctcggctct gacgcgctct gggaccagtc cgaatcgtat ctgcgcaccg cgctcaaccg gttctgcggc attccggang agctgcccga tccgttgcac ccggaccagc gctttcacta cgacggcaag ccggactcgg tcaagaagat gaaggcgctc atgaagaang nggagcgctg cgaggacccg aacgcgtgga anggcccgac caacggcggc gatggctgga tcgagaacnn ctggggangg cgccttctac ggccccanga tcgacatcgt cgtcnaggac gngctgcnnc cnccgcccac canngnnccn ncgatccagn ttggnnttcn anctnncnag ccnncttcgg ncncangtac accctnnngc cgcngannan gacnanacga ngnnnnancc cnngganann canancatgc cganccnngc nnnncnnnnn nnnnnccgnn ccgngnnnnn gnnnnnnnnn annnannccn nnncnnnnnn nangnntgnc
Expressed Protein Sequence
MAHHHHHHAK RDHRVIGRQQ NLFNFHEVSP GSAFWLPHGA RIYNTLVEFL RKKYRRCGFQ EVISPNMYSS KLWMVSGHWE KYADNMFCTK CEKEDFGLKP MNCPGHCIMF ASQPHSYKEL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QITDEMEGQM QFLSDVYGVL GFKFYFYHST RPANKLGSEA LWDQSESYLR TALNRFCGIP EELPDPLHPD QRFHYDGKPD SVKKMKALMK KAERCEDPNA WKGPTNGGDG WIENAGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPS RFGLKYTLPA AEKDETTVSP TEKQKHADPA LTASSPAVED CVSKPKPGTY EAAVRDLGID LELDANQARP VMIHRAILGS LERSIAILCE HFGGDWPFWL SPRQVMVVPV SASNYEYSQQ VRDTMHDAGF HADVDNGAAT LDKKIRNAEK ARYNFILVVG QKEQDATAVN IRARGEKRLG NKSLVEAVQW LKELADTHNR EY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGAAGC GCGACCACCG CGTAATTGGC CGCCAGCAGA ACCTCTTCAA CTTCCACGAG GTCTCACCTG GCAGCGCCTT CTGGCTGCCG CACGGCGCCC GGATCTACAA CACTCTCGTA GAATTCCTGC GCAAGAAGTA TCGCCGCTGC GGCTTTCAGG AGGTCATCTC GCCGAACATG TACTCCTCCA AACTTTGGAT GGTGTCGGGC CACTGGGAGA AGTACGCCGA TAACATGTTC TGCACCAAGT GCGAGAAGGA GGACTTTGGT CTGAAGCCGA TGAACTGCCC GGGCCACTGC ATCATGTTCG CGTCGCAGCC GCACTCGTAC AAGGAGCTGC CGATTCGCTA CGCCGACTTT GGCGTGCTGC ACCGGAACGA GCTGAGCGGT GCCTTGACGG GGCTGACCCG CGTGCGCCGC TTCCAGCAGG ACGATGCACA CATCTTCTGC CGCATGGACC AGATCACAGA TGAGATGGAG GGCCAGATGC AGTTCCTCAG CGACGTTTAC GGCGTGCTGG GCTTCAAGTT CTACTTTTAC CACTCCACCC GCCCCGCGAA CAAGCTCGGC TCTGAAGCGC TCTGGGACCA GTCCGAATCG TATCTGCGCA CCGCGCTCAA CCGGTTCTGC GGCATTCCGG AGGAGCTGCC CGATCCGTTG CACCCGGACC AGCGCTTTCA CTACGACGGC AAGCCGGACT CGGTCAAGAA GATGAAGGCG CTCATGAAGA AGGCGGAGCG CTGCGAGGAC CCGAACGCGT GGAAGGGCCC GACCAACGGC GGCGATGGCT GGATCGAGAA CGCTGGGGAC GGCGCCTTCT ACGGCCCCAA GATCGACATC GTCGTCGAGG ACGCGCTGCG CCGCCGCCAC CAGTGCGCCA CGATCCAGTT GGACTTCAAC CTGCCGAGCC GCTTCGGCCT CAAGTACACC CTTCCCGCCG CAGAGAAGGA CGAGACGACG GTGAGCCCAA CGGAGAAGCA GAAGCATGCC GACCCTGCCC TGACCGCGTC GTCGCCGGCC GTGGAGGATT GCGTGAGCAA ACCGAAGCCC GGCACGTACG AGGCTGCTGT GCGTGACCTT GGCATCGACC TCGAGCTGGA CGCCAACCAG GCCCGCCCCG TCATGATCCA CCGCGCCATT CTCGGTTCCT TGGAGCGCTC GATCGCCATT CTGTGCGAGC ACTTTGGCGG CGACTGGCCC TTCTGGCTGA GCCCCCGCCA AGTGATGGTG GTGCCTGTTT CGGCCTCCAA CTACGAGTAT TCACAGCAGG TGCGCGACAC GATGCACGAT GCCGGCTTCC ACGCGGACGT CGACAACGGT GCCGCCACGC TGGACAAGAA GATCCGCAAT GCGGAAAAGG CGCGCTACAA CTTTATCCTT GTCGTGGGCC AGAAGGAGCA GGACGCGACC GCCGTCAACA TCCGCGCGCG CGGGGAGAAG AGACTGGGCA ACAAGTCACT CGTGGAGGCG GTGCAGTGGC TCAAGGAGCT GGCGGACACA CATAACCGGG AGTACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT