LeinA.01510.b

Repressor of differentiation kinase 2, putative (RDK2)

CENTER ID: LeinA.01510.b
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.01510.b.B2.GE42793 PHoranyi kinase domain truncation 33 441
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.31.3070
UniProt: A4I7A1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEQDQRKPN GGNNGLPVPP STTETGQGPL QDILGRTPNC QYVKKRLLGQ GSFGSAWRVE ESATGNVFAA KVMDLNSMSE KDRGFVTNEV KCLSRCCNPH VVRCENAIER EGQLLIVMEY ADGGDLYKQI KARVREMKFF REHEVVFIFL QLCLALDHIH SNKMMHRDLK TANILLTTTG LVKLGDFGFS RQYEDSLSNA VGSTFCGTPY YLSPELWHRQ PYSKKSEMWA LGVVLYEVMA LRRPFTGKNM DELITNICHG RRSDLPSSYS ASLRDVCNRL LSVDSNTRPT IHELFHEPFI RSNLETLKRS VEKHNRIPAD VREAIARCIT QSLNTEEPPA HLEEPEPLIG TVKRHTALRG WQKCLLSFDK KQISIRDPSG VDPEEVVPVA TLTSVCPIDV MMAGEEFVFA MKNQAGKAFW FMAPTQRAYE QWLTTLQEAA P
NT Sequence
atggaggagc aagaccagcg aaagccgaac ggcggcaaca acggcctgcc agtgccaccc agcacgacag agaccggtca gggccctctg caggacatcc tcggccggac gccgaactgc cagtacgtca aaaagcgctt gctgggacag ggcagctttg gtagcgcgtg gcgcgtggag gaaagcgcga ccggcaacgt cttcgccgcg aaagtgatgg acctgaatag catgagcgag aaggaccgcg gcttcgtgac aaacgaggtg aagtgtctct cgcgctgctg caacccgcac gtcgtgcgtt gcgagaacgc catcgagaga gaggggcagc tgctgattgt gatggagtac gccgatggcg gggatctgta caagcagata aaggcccgcg tgcgcgagat gaagtttttc cgcgagcacg aggtggtgtt catctttctg cagctctgcc ttgccctgga ccacatccac tcgaacaaga tgatgcaccg cgacctgaag accgcaaaca tactcctcac taccaccggc ctcgtcaagc tcggcgactt cggcttttcg cgccagtacg aggactccct ctccaatgcc gtcggctcga ccttctgtgg cacgccatac tacctcagcc cggagttgtg gcatcgccag ccgtacagca agaagagtga aatgtgggcc ctcggagtcg tgctttacga ggtcatggcg ctccgccgcc ccttcaccgg caagaacatg gatgaactca tcacaaacat atgccacggg cggcgctctg acctgccgtc atcgtactcg gcgagcttgc gcgatgtgtg caaccgactt ctctccgtcg actccaacac gcggccgacg attcacgagc tgtttcacga gccttttatt cgctccaacc tcgagaccct caagcgcagt gtggaaaagc acaaccgcat ccctgccgac gtgcgcgagg ccatcgcgcg ctgcattacg cagtcgctga acacggagga accgcctgcg cacttggagg agccggagcc gctcattggc acggtgaagc gccacacggc gctgcgcgga tggcagaagt gcctcttgag tttcgacaag aagcagattt cgatccggga cccaagcggg gtggacccgg aagaggtggt gccggtcgcc actctgacct ccgtgtgccc catcgatgtc atgatggctg gcgaggagtt cgtctttgcc atgaagaacc aggcgggcaa ggccttttgg ttcatggcgc cgacccagag ggcatacgag cagtggctga ctacgttgca ggaagccgcc ccgtaa
Details for LeinA.01510.b.B2.GE42793
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 86
PERCENT COVERAGE: 48
Validated AA Sequence
MAHHHHHHMI LGRTPNCQYV KKRLLGQGXF GSAWRVEEXX XGNVFAAKVM DLNXMSXXDR GFVTNXVKCL SRCCNPHVVR XXNAXEREGQ LLIVMEYADG GDLYKQIKAR VREMXFFREH EVVFIFLXXC LALXHIHSNK MMHRDLKTAN ILLTTTGLVK LGDFGFXXQY EDSLSNAVGS TFCGTXILPQ PGVVASPAVQ QEE
Validated NT Sequence
agccactgct cgtacgccct ctgggtcggc gccatgaacc aaaaggcctt gcccgcctgg ttcttcatgg caaagacgaa ctcctcgcca gccatcatga catcgatggg gcacacggag gtcagagtgg cgaccggcac cacctcttcc gggtccaccc cgcttggatc ccggatcgaa atctgcttct tgtcgaaact caagaggcac ttctgccatc cgcgcagcgc cgtgtggcgc ttcaccgtgc caatgagcgg ctccggctcc tccaagtgcg caggcggttc ctccgtgttc agcgactgcg taatgcagcg cgcgatggcc tcgcgcacgt cggcagggat gcggttgtgc ttttccacgc tgcgcttgag ggtctcgagg ttggagcgaa taaaaggctc gtgaaacagc tcgtgaatcg tcggccgcgt gttggagtcg acggagagaa gtcggttgca cacatcgcgc aagctcgccg agtacgatga cggcaggtca gagcgccgcc cgtggcatat gtttgtgatg agttcatcca tgttcttgcc ggtgaagggg cggcggagcg ccatgacctc gtaaagcacg actccgaggg cccacatttc actcttcttg ctgtacggct ggcgatgcca caactccggg ctgaggtagt atgnnngtgc cacagaaggt cgagccgacg gcattggaga gggagtcctc gtactggnnn naaaagccga agtcgccgag cttgacgagg ccggtggtag tgaggagtat gtttgcggtc ttcaggtcgc ggtgcatcat cttgttcgag tggatgtggn ccagggcaag gcaganntgc agaaagatga acaccacctc gtgctcgcgg aaaaanntca tctcgcgcac gcgggccttt atctgcttgt acagatcccc gccatcggcg tactccatca cgatcagcag ctgcccctct ctctcnatgg cgttnnnnca acgcacgacg tgcgggttgc agcagcgcga gagacacttc acnnngtttg tcacgaaacc acggtccnnn tcgctcatnc tattcaggtc catcactttc gcggcgaaga cgttgccggn nnnnctttcc tccacgcgcc acgcgctacc aaannngccc tgtcccagca agcgcttttt gacgtactgg cagttcggcg tccggccgag gatcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHIL GRTPNCQYVK KRLLGQGSFG SAWRVEESAT GNVFAAKVMD LNSMSEKDRG FVTNEVKCLS RCCNPHVVRC ENAIEREGQL LIVMEYADGG DLYKQIKARV REMKFFREHE VVFIFLQLCL ALDHIHSNKM MHRDLKTANI LLTTTGLVKL GDFGFSRQYE DSLSNAVGST FCGTPYYLSP ELWHRQPYSK KSEMWALGVV LYEVMALRRP FTGKNMDELI TNICHGRRSD LPSSYSASLR DVCNRLLSVD SNTRPTIHEL FHEPFIRSNL ETLKRSVEKH NRIPADVREA IARCITQSLN TEEPPAHLEE PEPLIGTVKR HTALRGWQKC LLSFDKKQIS IRDPSGVDPE EVVPVATLTS VCPIDVMMAG EEFVFAMKNQ AGKAFWFMAP TQRAYEQWLT TLQEAAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcctcg gccggacgcc gaactgccag tacgtcaaaa agcgcttgct gggacagggc agctttggta gcgcgtggcg cgtggaggaa agcgcgaccg gcaacgtctt cgccgcgaaa gtgatggacc tgaatagcat gagcgagaag gaccgcggct tcgtgacaaa cgaggtgaag tgtctctcgc gctgctgcaa cccgcacgtc gtgcgttgcg agaacgccat cgagagagag gggcagctgc tgattgtgat ggagtacgcc gatggcgggg atctgtacaa gcagataaag gcccgcgtgc gcgagatgaa gtttttccgc gagcacgagg tggtgttcat ctttctgcag ctctgccttg ccctggacca catccactcg aacaagatga tgcaccgcga cctgaagacc gcaaacatac tcctcactac caccggcctc gtcaagctcg gcgacttcgg cttttcgcgc cagtacgagg actccctctc caatgccgtc ggctcgacct tctgtggcac gccatactac ctcagcccgg agttgtggca tcgccagccg tacagcaaga agagtgaaat gtgggccctc ggagtcgtgc tttacgaggt catggcgctc cgccgcccct tcaccggcaa gaacatggat gaactcatca caaacatatg ccacgggcgg cgctctgacc tgccgtcatc gtactcggcg agcttgcgcg atgtgtgcaa ccgacttctc tccgtcgact ccaacacgcg gccgacgatt cacgagctgt ttcacgagcc ttttattcgc tccaacctcg agaccctcaa gcgcagtgtg gaaaagcaca accgcatccc tgccgacgtg cgcgaggcca tcgcgcgctg cattacgcag tcgctgaaca cggaggaacc gcctgcgcac ttggaggagc cggagccgct cattggcacg gtgaagcgcc acacggcgct gcgcggatgg cagaagtgcc tcttgagttt cgacaagaag cagatttcga tccgggaccc aagcggggtg gacccggaag aggtggtgcc ggtcgccact ctgacctccg tgtgccccat cgatgtcatg atggctggcg aggagttcgt ctttgccatg aagaaccagg cgggcaaggc cttttggttc atggcgccga cccagagggc atacgagcag tggctgacta cgttgcagga agccgccccg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat