SSGCID: Target Page

LeinA.01238.a

seryl-tRNA synthetase

CENTER ID:	LeinA.01238.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.01238.a.B1.GE42527	full length		1	474
LeinA.01238.a.AE1.GE42991	full length		1	474

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.01238.a.B1.PW38515	full length		1	474

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_88902

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LinJ.11.0100
UniProt:	A4HUS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLDIQLFRD PEMADIVRES ERRRYARPEI VDDIIEIDKR WRRTQFLTEA SKKMINTCSK AVGAKKKAKE ADGDVSEVPA DVMSAAREGT LDTEKLESLC ILQLKQLSKA LATQVESLAK LAAEQEAERD RMVISVGNVL HESVPVSNDE ETGNVVVRTF GDVTRRLKMT HVDCMERLGL MDTSKAVTAM AGGRAFVLRG GLVQLQFALI SYALNFLIAR GYEPFYPPFF LNKEYMGAVA QLSDFDESLY KVSGDGDEKY LIATSEAPIA AYHTNKWFTE MKEPIKYAGV SSCFRKEAGA HGRDTLGIFR VHQFDKLEQF VVCSPREDQS WKMLEEMIQT SQDFNESLGL PYRVINICSG ALNNAAAKKY DLEAWFPGSG AFRELVSCSN CTDYQARSVN CRFGPNTKGS TANSTKEYCH MLNGTLCAVT RTMCCICENY QTEEGILIPE VLRPFMMGTE MLKFPAEAPT ADKE

NT Sequence

atggggctcg acattcagct gttccgcgat cccgagatgg cggatatagt gcgcgagtcg gagcgccgcc gctacgccag gccggagatc gtcgatgata tcatagagat tgacaagcgc tggcgccgca cgcagtttct aacggaagcc agcaagaaaa tgatcaacac ttgcagcaaa gccgtcggcg ccaagaagaa ggcaaaggag gcggacggcg acgtctctga agtgccggca gatgtcatga gcgccgcccg ggaaggcaca ttagacacgg agaagctgga gtcgttgtgc attctccagc taaagcagct gtccaaggca ctggcgacgc aggtggagag tcttgcaaag ctggccgcgg agcaggaagc ggagcgggat cgtatggtga ttagcgttgg caacgtcttg cacgagtctg tcccggtctc gaacgacgag gaaaccggca acgtggtcgt gcgcaccttt ggtgacgtga cgcgccgctt gaagatgacg catgtagact gcatggagcg tctgggactt atggacacgt ccaaggcggt aaccgcaatg gcgggtggcc gcgcatttgt tctgcgtggt ggcctcgtac agctgcaatt cgccctcatc tcgtacgcgc tcaacttcct catcgcccgc ggctacgagc ccttctaccc gcccttcttc ctcaacaagg agtatatggg tgcagtggcg cagctttctg actttgacga gtcgctctac aaggtttccg gcgacgggga cgagaagtac ctgattgcga cgagcgaggc acccattgcc gcctaccaca ccaacaagtg gttcaccgaa atgaaggagc cgatcaagta cgccggcgtc tcgtcgtgct tccgaaagga ggccggtgcg cacggccgcg acacgctcgg catcttccgc gtccaccagt tcgacaaact ggagcagttt gttgtgtgct ctcctcgcga agatcagtcg tggaagatgc tggaagagat gatccagacc tcccaggact tcaacgagag cctcggtctg ccgtaccgcg tcatcaacat ctgctctggg gccctcaaca atgctgctgc caagaagtac gacctggagg cgtggttccc cggctctggt gcattccgcg agcttgtttc gtgctcgaac tgcacggact accaggcccg cagtgttaac tgccgcttcg gcccgaacac aaagggcagc acagcgaaca gcacgaagga atactgccac atgctgaacg gtactctctg cgctgtcact cgcacaatgt gctgcatctg cgaaaactac cagaccgagg aaggcatctt aatccccgag gtactgcgcc cctttatgat ggggacggag atgctcaagt tcccggcaga agcccctacg gccgataagg agtaa

Details for LeinA.01238.a.B1.GE42527

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	54kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG LDIQLFRDPE MADIVRESER RRYARPEIVD DIIEIDKRWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDVSEVPADV MSAAREGTLD TEKLESLCIL QLKQLSKALA TQVESLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDEET GNVVVRTFGD VTRRLKMTHV DCMERLGLMD TSKAVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SDFDESLYKX SGDGDEKYLI ATSEAPIAAY RTNKWFTEMK EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMIQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NSTKEYCHML NGTLCAVTRT MCCICENYQT EEGIVIPEVL RPFMMGTEML KFPXXXXTAD KE

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG LDIQLFRDPE MADIVRESER RRYARPEIVD DIIEIDKRWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDVSEVPADV MSAAREGTLD TEKLESLCIL QLKQLSKALA TQVESLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDEET GNVVVRTFGD VTRRLKMTHV DCMERLGLMD TSKAVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SDFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMK EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMIQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NSTKEYCHML NGTLCAVTRT MCCICENYQT EEGILIPEVL RPFMMGTEML KFPAEAPTAD KE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc tcgacattca gctgttccgc gatcccgaga tggcggatat agtgcgcgag tcggagcgcc gccgctacgc caggccggag atcgtcgatg atatcataga gattgacaag cgctggcgcc gcacgcagtt tctaacggaa gccagcaaga aaatgatcaa cacttgcagc aaagccgtcg gcgccaagaa gaaggcaaag gaggcggacg gcgacgtctc tgaagtgccg gcagatgtca tgagcgccgc ccgggaaggc acattagaca cggagaagct ggagtcgttg tgcattctcc agctaaagca gctgtccaag gcactggcga cgcaggtgga gagtcttgca aagctggccg cggagcagga agcggagcgg gatcgtatgg tgattagcgt tggcaacgtc ttgcacgagt ctgtcccggt ctcgaacgac gaggaaaccg gcaacgtggt cgtgcgcacc tttggtgacg tgacgcgccg cttgaagatg acgcatgtag actgcatgga gcgtctggga cttatggaca cgtccaaggc ggtaaccgca atggcgggtg gccgcgcatt tgttctgcgt ggtggcctcg tacagctgca attcgccctc atctcgtacg cgctcaactt cctcatcgcc cgcggctacg agcccttcta cccgcccttc ttcctcaaca aggagtatat gggtgcagtg gcgcagcttt ctgactttga cgagtcgctc tacaaggttt ccggcgacgg ggacgagaag tacctgattg cgacgagcga ggcacccatt gccgcctacc acaccaacaa gtggttcacc gaaatgaagg agccgatcaa gtacgccggc gtctcgtcgt gcttccgaaa ggaggccggt gcgcacggcc gcgacacgct cggcatcttc cgcgtccacc agttcgacaa actggagcag tttgttgtgt gctctcctcg cgaagatcag tcgtggaaga tgctggaaga gatgatccag acctcccagg acttcaacga gagcctcggt ctgccgtacc gcgtcatcaa catctgctct ggggccctca acaatgctgc tgccaagaag tacgacctgg aggcgtggtt ccccggctct ggtgcattcc gcgagcttgt ttcgtgctcg aactgcacgg actaccaggc ccgcagtgtt aactgccgct tcggcccgaa cacaaagggc agcacagcga acagcacgaa ggaatactgc cacatgctga acggtactct ctgcgctgtc actcgcacaa tgtgctgcat ctgcgaaaac taccagaccg aggaaggcat cttaatcccc gaggtactgc gcccctttat gatggggacg gagatgctca agttcccggc agaagcccct acggccgata aggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for LeinA.01238.a.AE1.GE42991

HARVESTED ON:	3/4/2019
SEQUENCED ON:	3/12/2019
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggggctcgac attcagctgt tccgcgatcc cgagatggcg gatatagtgc gcgagtcgga gcgccgccgc tacgccaggc cggagatcgt cgatgatatc atagagattg acaagcgctg gcgccgcacg cagtttctaa cggaagccag caagaaaatg atcaacactt gcagcaaagc cgtcggcgcc aagaagaagg caaaggaggc ggacggcgac gtctctgaag tgccggcaga tgtcatgagc gccgcccggg aaggcacatt agacacggag aagctggagt cgttgtgcat tctccagcta aagcagctgt ccaaggcact ggcgacgcag gtggagagtc ttgcaaagct ggccgcggag caggaagcgg agcgggatcg tatggtgatt agcgttggca acgtcttgca cgagtctgtc ccggtctcga acgacgagga aaccggcaac gtggtcgtgc gcacctttgg tgacgtgacg cgccgcttga agatgacgca tgtagactgc atggagcgtc tgggacttat ggacacgtcc aaggcggtaa ccgcaatggc gggtggccgc gcatttgttc tgcgtggtgg cctcgtacag ctgcaattcg ccctcatctc gtacgcgctc aacttcctca tcgcccgcgg ctacgagccc ttctacccgc ccttcttcct caacaaggag tatatgggtg cagtggcgca gctttctgac tttgacgagt cgctctacaa ggtttccggc gacggggacg agaagtacct gattgcgacg agcgaggcac ccattgccgc ctaccacacc aacaagtggt tcaccgaaat gaaggagccg atcaagtacg ccggcgtctc gtcgtgcttc cgaaaggagg ccggtgcgca cggccgcgac acgctcggca tcttccgcgt ccaccagttc gacaaactgg agcagtttgt tgtgtgctct cctcgcgaag atcagtcgtg gaagatgctg gaagagatga tccagacctc ccaggacttc aacgagagcc tcggtctgcc gtaccgcgtc atcaacatct gctctggggc cctcaacaat gctgctgcca agaagtacga cctggaggcg tggttccccg gctctggtgc attccgcgag cttgtttcgt gctcgaactg cacggactac caggcccgca gtgttaactg ccgcttcggc ccgaacacaa agggcagcac agcgaacagc acgaaggaat actgccacat gctgaacggt actctctgcg ctgtcactcg cacaatgtgc tgcatctgcg aaaactacca gaccgaggaa ggcatcttaa tccccgaggt actgcgcccc tttatgatgg ggacggagat gctcaagttc ccggcagaag cccctacggc cgataaggag gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat

Details for LeinA.01238.a.B1.PW38515

PURIFICATION DATe:	7/23/2018
CONCENTRATION:	11.15mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG LDIQLFRDPE MADIVRESER RRYARPEIVD DIIEIDKRWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDVSEVPADV MSAAREGTLD TEKLESLCIL QLKQLSKALA TQVESLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDEET GNVVVRTFGD VTRRLKMTHV DCMERLGLMD TSKAVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SDFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMK EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMIQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NSTKEYCHML NGTLCAVTRT MCCICENYQT EEGILIPEVL RPFMMGTEML KFPAEAPTAD KE

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGGC TCGACATTCA GCTGTTCCGC GATCCCGAGA TGGCGGATAT AGTGCGCGAG TCGGAGCGCC GCCGCTACGC CAGGCCGGAG ATCGTCGATG ATATCATAGA GATTGACAAG CGCTGGCGCC GCACGCAGTT TCTAACGGAA GCCAGCAAGA AAATGATCAA CACTTGCAGC AAAGCCGTCG GCGCCAAGAA GAAGGCAAAG GAGGCGGACG GCGACGTCTC TGAAGTGCCG GCAGATGTCA TGAGCGCCGC CCGGGAAGGC ACATTAGACA CGGAGAAGCT GGAGTCGTTG TGCATTCTCC AGCTAAAGCA GCTGTCCAAG GCACTGGCGA CGCAGGTGGA GAGTCTTGCA AAGCTGGCCG CGGAGCAGGA AGCGGAGCGG GATCGTATGG TGATTAGCGT TGGCAACGTC TTGCACGAGT CTGTCCCGGT CTCGAACGAC GAGGAAACCG GCAACGTGGT CGTGCGCACC TTTGGTGACG TGACGCGCCG CTTGAAGATG ACGCATGTAG ACTGCATGGA GCGTCTGGGA CTTATGGACA CGTCCAAGGC GGTAACCGCA ATGGCGGGTG GCCGCGCATT TGTTCTGCGT GGTGGCCTCG TACAGCTGCA ATTCGCCCTC ATCTCGTACG CGCTCAACTT CCTCATCGCC CGCGGCTACG AGCCCTTCTA CCCGCCCTTC TTCCTCAACA AGGAGTATAT GGGTGCAGTG GCGCAGCTTT CTGACTTTGA CGAGTCGCTC TACAAGGTTT CCGGCGACGG GGACGAGAAG TACCTGATTG CGACGAGCGA GGCACCCATT GCCGCCTACC ACACCAACAA GTGGTTCACC GAAATGAAGG AGCCGATCAA GTACGCCGGC GTCTCGTCGT GCTTCCGAAA GGAGGCCGGT GCGCACGGCC GCGACACGCT CGGCATCTTC CGCGTCCACC AGTTCGACAA ACTGGAGCAG TTTGTTGTGT GCTCTCCTCG CGAAGATCAG TCGTGGAAGA TGCTGGAAGA GATGATCCAG ACCTCCCAGG ACTTCAACGA GAGCCTCGGT CTGCCGTACC GCGTCATCAA CATCTGCTCT GGGGCCCTCA ACAATGCTGC TGCCAAGAAG TACGACCTGG AGGCGTGGTT CCCCGGCTCT GGTGCATTCC GCGAGCTTGT TTCGTGCTCG AACTGCACGG ACTACCAGGC CCGCAGTGTT AACTGCCGCT TCGGCCCGAA CACAAAGGGC AGCACAGCGA ACAGCACGAA GGAATACTGC CACATGCTGA ACGGTACTCT CTGCGCTGTC ACTCGCACAA TGTGCTGCAT CTGCGAAAAC TACCAGACCG AGGAAGGCAT CTTAATCCCC GAGGTACTGC GCCCCTTTAT GATGGGGACG GAGATGCTCA AGTTCCCGGC AGAAGCCCCT ACGGCCGATA AGGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT