LeinA.01171.a

Putative nucleolar RNA helicase II (LinJ.05.0140)

CENTER ID: LeinA.01171.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.01171.a.B2.GE37536 DEAD-box RNA helicase 78 466
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LinJ.05.0140
RefSeq: XP_001462984.2
UniProt: A4HSE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRRAREESE EEKPHRITKL AGSSDDDVPV KASKISAKAS DHGNGVAVEK RKRTEGSCKV AVTSATAPAE EKDEEVPNNG CAATARPFSE FEMNPIVVKA LQSRGIESMF PVQALTFNAI MRNTDVLVQA RTGSGKTLAF GIPIVERLLK LPSHFTRGRG PAAVIFCPTR ELAIQVQDVL CGISCGLVVT ALYGGVAYAN QERVLRSGVD IVVATPGRAK DFLEKGTLHF DRVVMACLDE ADHMLDIGFK DDIELLLSQV AEQNGSVGAE RPVHQTLLFS ATVPEWVHTC SFIAKDKEFI DMVGKEAVRT ASTIKFYRRK CNFSEISSML ADLIKVYSGA HGRTLVFTNT KKDCHDLSIN NTKLDSQCLH GDMQQEQRES TMKSFRDNKF SVLIATDVAA RGLDLPMVDL VIQCAPPSDI DAFIHRAGRT GRAGRKGVCV LLYQPREEYV VERIERHAKM KFDVLPAPTR EEILKAVARD AAEDLARVER RATDLFMDQA AELLKDADPV EILASALAVM SGYTSNITTR GLITGTPGYV TVQMTSDRPL PVPVYCSILR NNLGDETFMR CRDITLLQDD PGCVFDVLEQ FAERVMSTPM RGITFQRIET LPPIIARELN SSRGGRSGFN YGGGRGGFNN GGGYGSGRGG FGGYGGGRGG GRGGGRGGGR GGGFQRRY
NT Sequence
atgccgcgtc gcgcgcgcga ggagagtgag gaggagaaac cgcaccgcat cacgaagctc gccggcagca gcgacgacga tgtgccggtg aaggcgtcga agatctccgc caaggcgtcg gaccacggca acggcgttgc tgtagagaaa cggaagcgca cggaggggag ctgcaaggtt gctgtcactt cggcgacggc accggcggag gagaaggacg aggaggtgcc gaacaacggc tgcgccgcca cagcgcgccc gttctcggag ttcgagatga accccatcgt cgtcaaggcg ctgcagtcac gcggcatcga gtctatgttt cctgtgcagg ctctcacctt taacgccatc atgaggaaca cggacgtcct cgtgcaggcc cgcaccggca gcggcaagac actcgcgttc ggtatcccga tcgtggagcg gctgctgaag ctgccgagcc acttcacccg cggccgcggc ccggctgccg tgatcttctg ccctactcgt gagctggcca tccaggtgca ggatgtgctc tgcgggatca gctgcgggct cgtcgtgacg gccctgtacg gcggtgttgc ctacgcgaat caggagcgcg tgctgcgcag cggcgtcgac attgtggtgg ccacccccgg ccgcgcgaag gactttctag agaagggcac gctgcatttt gaccgcgttg tgatggcgtg cctggacgag gcggaccaca tgctggacat cggcttcaag gacgacattg agttgctgct ctcgcaggtg gccgagcaga acggctctgt cggcgctgaa aggccagttc atcagacgct gctgttctcc gcgacggtgc cggagtgggt gcacacgtgc tccttcatcg ccaaggacaa ggagttcatc gacatggtcg gcaaggaggc tgtgcggaca gcgagcacga tcaagttcta tcgcagaaaa tgcaacttca gcgagatctc ctctatgctc gctgatctca tcaaggtgta cagcggtgcc cacggccgca ctctcgtctt caccaacaca aagaaggact gccacgactt gtccatcaac aacaccaaac tggactcgca gtgcctgcac ggtgatatgc agcaggagca gcgcgagagt accatgaaga gcttccgcga caacaagttc agcgtcctca tcgccaccga cgtggcggcc cgcggtctcg acctgcccat ggtggacctg gttattcagt gcgctccacc gagcgacatc gacgctttca ttcaccgcgc cggccgtacg ggacgcgctg gccgcaaggg tgtgtgcgtg ctgctctacc aaccgcgcga ggagtacgtt gtcgagcgca tcgagcgcca cgcgaaaatg aagtttgacg tcctgccggc acccacgcgt gaagagattc tcaaggccgt cgcccgtgat gccgctgagg acctggcgcg cgtcgagcgc cgtgcaaccg acctcttcat ggatcaggca gccgagctgc tgaaggacgc agacccagtc gaaatcctgg cctcggcgct cgccgtcatg agcggctaca cgtcgaacat caccacgcgc ggtctcatta ctggcacgcc agggtatgtg acggtgcaga tgacgtcgga tcgaccgctg cccgttccgg tgtactgcag catcctccga aacaacctcg gcgatgaaac gttcatgcgg tgccgcgaca ttacactgtt gcaggacgac ccgggctgcg tcttcgatgt gctggagcag tttgccgagc gtgtcatgag cacaccgatg cgcggtatca ccttccagcg gatcgagacc cttcctccga tcatcgcgcg cgagctcaac agcagccgcg gcggtcgcag tggctttaac tacggaggcg gtcgcggtgg cttcaataac ggcggcggct acggcagtgg gcgtggtggc ttcggcggct acgggggggg tcgcggcggc ggtcgcggcg gcggccgcgg cggtgggcgt ggtggcggtt tccagcgccg ctactga
Details for LeinA.01171.a.B2.GE37536
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MNPIVVKALQ SRGIESMFPV QALTFNAIMR NTDVLVQART GSGKTLAFGI PIVERLLKLP SHFTRGRGPA AVIFCPTREL AIQVQDVLCG ISCGLVVTAL YGGVAYANQE RVLRSGVDIV VATPGRAKDF LEKGTLHFDR VVMACLDEAD HMLDIGFKDD IELLLSQVAE QNGSVGAERP VHQTLLFSAT VPEWVHTCSF IAKDKEFIDM VGKEAVRTAS TIKFYRRKCN FSEISSMLAD LIKVYSGAHG RTLVFTNTKK DCHDLSINNT KLDSQCLHGD MQQEQRESTM KSFRDNKFSV LIATDVAARG LDLPMVDLVI QCAPPSDIDA FIHRAGRTGR AGRKGVCVLL YQPREEYVVE RIERHAKMKF DVLP
Validated NT Sequence
atcttactca cttacggcag gacgtcaaac ttcattttcg cgtggcgctc gatgcgctcg acaacgtact cctcgcgcgg ttggtagagc agcacgcaca cacccttgcg gccagcgcgt cccgtacggc cggcgcggtg aatgaaagcg tcgatgtcgc tcggtggagc gcactgaata accaggtcca ccatgggcag gtcgagaccg cgggccgcca cgtcggtggc gatgaggacg ctgaacttgt tgtcgcggaa gctcttcatg gtactctcgc gctgctcctg ctgcatatca ccgtgcaggc actgcgagtc cagtttggtg ttgttgatgg acaagtcgtg gcagtccttc tttgtgttgg tgaagacgag agtgcggccg tgggcaccgc tgtacacctt gatgagatca gcgagcatag aggagatctc gctgaagttg cattttctgc gatagaactt gatcgtgctc gctgtccgca cagcctcctt gccgaccatg tcgatgaact ccttgtcctt ggcgatgaag gagcacgtgt gcacccactc cggcaccgtc gcggagaaca gcagcgtctg atgaactggc ctttcagcgc cgacagagcc gttctgctcg gccacctgcg agagcagcaa ctcaatgtcg tccttgaagc cgatgtccag catgtggtcc gcctcgtcca ggcacgccat cacaacgcgg tcaaaatgca gcgtgccctt ctctagaaag tccttcgcgc ggccgggggt ggccaccaca atgtcgacgc cgctgcgcag cacgcgctcc tgattcgcgt aggcaacacc gccgtacagg gccgtcacga cgagcccgca gctgatcccg cagagcacat cctgcacctg gatggccagc tcacgagtag ggcagaagat cacggcagcc gggccgcggc cgcgggtgaa gtggctcggc agcttcagca gccgctccac gatcgggata ccgaacgcga gtgtcttgcc gctgccggtg cgggcctgca cgaggacgtc cgtgttcctc atgatggcgt taaaggtgag agcctgcaca ggaaacatag actcgatgcc gcgtgactgc agcgccttga cgacgatggg gttcatctcg aactccgaga acgggcgcgc tgtggcggcg cagccgttg
Expected Protein Sequence
MAHHHHHHNN GCAATARPFS EFEMNPIVVK ALQSRGIESM FPVQALTFNA IMRNTDVLVQ ARTGSGKTLA FGIPIVERLL KLPSHFTRGR GPAAVIFCPT RELAIQVQDV LCGISCGLVV TALYGGVAYA NQERVLRSGV DIVVATPGRA KDFLEKGTLH FDRVVMACLD EADHMLDIGF KDDIELLLSQ VAEQNGSVGA ERPVHQTLLF SATVPEWVHT CSFIAKDKEF IDMVGKEAVR TASTIKFYRR KCNFSEISSM LADLIKVYSG AHGRTLVFTN TKKDCHDLSI NNTKLDSQCL HGDMQQEQRE STMKSFRDNK FSVLIATDVA ARGLDLPMVD LVIQCAPPSD IDAFIHRAGR TGRAGRKGVC VLLYQPREEY VVERIERHAK MKFDVLP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacaacg gctgcgccgc cacagcgcgc ccgttctcgg agttcgagat gaaccccatc gtcgtcaagg cgctgcagtc acgcggcatc gagtctatgt ttcctgtgca ggctctcacc tttaacgcca tcatgaggaa cacggacgtc ctcgtgcagg cccgcaccgg cagcggcaag acactcgcgt tcggtatccc gatcgtggag cggctgctga agctgccgag ccacttcacc cgcggccgcg gcccggctgc cgtgatcttc tgccctactc gtgagctggc catccaggtg caggatgtgc tctgcgggat cagctgcggg ctcgtcgtga cggccctgta cggcggtgtt gcctacgcga atcaggagcg cgtgctgcgc agcggcgtcg acattgtggt ggccaccccc ggccgcgcga aggactttct agagaagggc acgctgcatt ttgaccgcgt tgtgatggcg tgcctggacg aggcggacca catgctggac atcggcttca aggacgacat tgagttgctg ctctcgcagg tggccgagca gaacggctct gtcggcgctg aaaggccagt tcatcagacg ctgctgttct ccgcgacggt gccggagtgg gtgcacacgt gctccttcat cgccaaggac aaggagttca tcgacatggt cggcaaggag gctgtgcgga cagcgagcac gatcaagttc tatcgcagaa aatgcaactt cagcgagatc tcctctatgc tcgctgatct catcaaggtg tacagcggtg cccacggccg cactctcgtc ttcaccaaca caaagaagga ctgccacgac ttgtccatca acaacaccaa actggactcg cagtgcctgc acggtgatat gcagcaggag cagcgcgaga gtaccatgaa gagcttccgc gacaacaagt tcagcgtcct catcgccacc gacgtggcgg cccgcggtct cgacctgccc atggtggacc tggttattca gtgcgctcca ccgagcgaca tcgacgcttt cattcaccgc gccggccgta cgggacgcgc tggccgcaag ggtgtgtgcg tgctgctcta ccaaccgcgc gaggagtacg ttgtcgagcg catcgagcgc cacgcgaaaa tgaagtttga cgtcctgccg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat