SSGCID: Target Page

LeinA.01169.a

protein phosphatase 1 catalytic subunit, putative (PP1C)

CENTER ID:	LeinA.01169.a
ORGANISM:	Leishmania infantum
ASSOCIATED DISEASE:	Visceral leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LeinA.01169.a.B1.GE42326	full length		1	374

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LinJ.15.0240
UniProt:	A4HWC1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAHTKRGRFT VDLSMCTLSS LPTDTLSVEK ENDVLYRLLE LLPERYTSKV LEEQRIPEGL IVQILIKARA IIASEPMVVE VDSPVYACGD LHGQYYDLVN IFKRCPPLGG TVFGTDAKKA RKETSSSIDD KTYSFLFLGD YVDRGARSVE VVVTLLALKI ISPRHMTMLR GNHEDEQIML LYGFYDECKR RYGIKLFKMF TDFFRVLPVA ALVNGSIFCV HGGLSAELRF VRDIPDTRPC NVPHSGIICD LLWADPETDL PAGVDWAPSS RRISSVFSER ALERFLVEND IDLVCRAHQV VEEGFQFFPD HKKRHLLTIF SATNYCNEFG NRGGILRVDE KGVCSIITLE PPDFVQQRDI MLFRDPLPNP LSHD

NT Sequence

atggcacaca cgaagcgagg gagattcacc gtggatctgt ccatgtgtac gctcagtagc cttcctacag atactctctc tgtggaaaaa gagaatgatg tgctttaccg ccttttagaa cttcttcccg agcgctacac gagcaaagtt ttggaagagc agcgcattcc tgagggcctc atcgtgcaga ttctcatcaa ggcacgcgct ataatagcca gtgagccgat ggttgtcgaa gtagattccc ctgtttacgc ttgcggtgat ctacacgggc agtactacga cttggttaac attttcaagc ggtgcccacc gcttggaggc accgttttcg gaactgatgc gaagaaggca cgtaaagaga ccagctcatc gatcgatgat aaaacataca gctttctgtt ccttggcgat tatgtcgacc gcggcgctcg ctccgttgag gtcgttgtga cactgctcgc tctcaagatt atttctccgc ggcacatgac tatgcttcgc ggaaaccacg aggacgaaca aatcatgctc ttgtatggat tttacgacga atgcaagaga cgctatggca ttaaactttt caaaatgttc accgattttt ttcgtgttct gcctgtggcg gccctagtga acggctctat tttctgcgtt cacggcggtc tctccgcgga gctccgcttc gtgcgagaca tcccggacac gcgcccgtgc aacgtcccgc actccggcat catttgtgac cttctgtggg cagatcccga aactgatctg ccagctggtg tggactgggc tccgagctcg cgcagaattt catctgtgtt ttccgaacgg gcactggagc ggtttttagt tgagaacgac attgatttgg tgtgccgcgc acatcaagtg gtcgaagaag gattccagtt tttcccggat cacaaaaagc ggcatttgct caccatcttt tcggcaacta actactgcaa cgagtttggg aatagaggcg gcattttacg cgtggatgag aagggtgtat gcagcatcat cacgttggaa ccaccagact ttgtccagca gcgggatatc atgctttttc gggatccgct tccaaaccct ctgagccacg actga

Details for LeinA.01169.a.B1.GE42326

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA HTKRGRFTVD LSMCTLSSLP TDTLSVEKEN DVLYRLLELL PERYTSKVLE EQRIPEGLIV QILIKARAII ASEPMVVEVD SPVYACGDLH GQYYDLVNIF KRCPPLGGTV FGTDAKKARK ETSSSIDDKT YSFLFLGDYV DRGARSVEVV VTLLALKIIS PRHMTMLRGN HEDEQIMLLY GFYDECKRRY GIKLFKMFTD FFRVLPVAAL VNGSIFCVHG GLSAELRFVR DIPDTRPCNV PHSGIICDLL WADPETDLPA GVDWAPSSRR ISSVFSERAL ERFLVENDID LVCRAHQVVE EGFQFFPDHK KRHLLTIFSA TNYCNEFGNR GGILRVDEKG VCSIITLEPP DFVQQRDIML FRD

Validated NT Sequence

ggatcccgaa aaagcatgat atcccgctgc tggacaaagt ctggtggttc caacgtgatg atgctgcata cacccttctc atccacgcgt aaaatgccgc ctctattccc aaactcgttg cagtagttag ttgccgaaaa gatggtgagc aaatgccgct ttttgtgatc cgggaaaaac tggaatcctt cttcgaccac ttgatgtgcg cggcacacca aatcaatgtc gttctcaact aaaaaccgct ccagtgcccg ttcggaaaac acagatgaaa ttctgcgcga gctcggagcc cagtccacac cagctggcag atcagtttcg ggatctgccc acagaaggtc acaaatgatg ccggagtgcg ggacgttgca cgggcgcgtg tccgggatgt ctcgcacgaa gcggagctcc gcggagagac cgccgtgaac gcagaaaatg gagccgttca ctagggccgc cacaggcaga acacgaaaaa aatcggtgaa cattttgaaa agtttaatgc catagcgtct cttgcattcg tcgtaaaatc catacaagag catgatttgt tcgtcctcgt ggtttccgcg aagcatagtc atgtgccgcg gagaaataat cttgagagcg agcagtgtca caacgacctc aacggagcga gcgccgcggt cgacataatc gccaaggaac agaaagctgt atgttttatc atcgatcgat gagctggtct ctttacgtgc cttcttcgca tcagttccga aaacggtgcc tccaagcggt gggcaccgct tgaaaatgtt aaccaagtcg tagtactgcc cgtgtagatc accgcaagcg taaacagggg aatctacttc gacaaccatc ggctcactgg ctattatagc gcgtgccttg atgagaatct gcacgatgag gccctcagga atgcgctgct cttccaaaac tttgctcgtg tagcgctcgg gaagaagttc taaaaggcgg taaagcacat cattctcttt ttccacagag agagtatctg taggaaggct actgagcgta cacatggaca gatccacggt gaatctccct cgcttcgtgt gtgccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac acacgaagcg agggagattc accgtggatc tgtccatgtg tacgctcagt agccttccta cagatactct ctctgtggaa aaagagaatg atgtgcttta ccgcctttta gaacttcttc ccgagcgcta cacgagcaaa gttttggaag agcagcgcat tcctgagggc ctcatcgtgc agattctcat caaggcacgc gctataatag ccagtgagcc gatggttgtc gaagtagatt cccctgttta cgcttgcggt gatctacacg ggcagtacta cgacttggtt aacattttca agcggtgccc accgcttgga ggcaccgttt tcggaactga tgcgaagaag gcacgtaaag agaccagctc atcgatcgat gataaaacat acagctttct gttccttggc gattatgtcg accgcggcgc tcgctccgtt gaggtcgttg tgacactgct cgctctcaag attatttctc cgcggcacat gactatgctt cgcggaaacc acgaggacga acaaatcatg ctcttgtatg gattttacga cgaatgcaag agacgctatg gcattaaact tttcaaaatg ttcaccgatt tttttcgtgt tctgcctgtg gcggccctag tgaacggctc tattttctgc gttcacggcg gtctctccgc ggagctccgc ttcgtgcgag acatcccgga cacgcgcccg tgcaacgtcc cgcactccgg catcatttgt gaccttctgt gggcagatcc cgaaactgat ctgccagctg gtgtggactg ggctccgagc tcgcgcagaa tttcatctgt gttttccgaa cgggcactgg agcggttttt agttgagaac gacattgatt tggtgtgccg cgcacatcaa gtggtcgaag aaggattcca gtttttcccg gatcacaaaa agcggcattt gctcaccatc ttttcggcaa ctaactactg caacgagttt gggaatagag gcggcatttt acgcgtggat gagaagggtg tatgcagcat catcacgttg gaaccaccag actttgtcca gcagcgggat atcatgcttt ttcgggatcc gcttccaaac cctctgagcc acgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat